Laurea in Biotecnologie

Academic program

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Year Course ID Course Teachers SSD Curriculum Site CFU
1 MS0729 Fundamentals of mathematics and physics TRIVERO Paolo, PANZIERI Daniele, RINALDI Maurizio, GASTALDI Fabio, MARTIGNONE Francesca FIS/01, MAT/04 All NOVARA 12
1 MS0734 Genetics SOLURI Angela Maria Felicia, PERSICHETTI Francesca, GIORDANO Mara, CORRADO Lucia BIO/18, MED/03 All NOVARA 9
1 MS0731 Histology and anatomy PRAT Maria Giovanna, COTELLA Diego, BOZZO Chiarella, FOLLENZI Antonia, BOCCAFOSCHI Francesca, SABBATINI Maurizio BIO/13, BIO/17, BIO/16 All NOVARA 15
1 MS0725 Inorganic chemistry BOCCALERI Enrico, BOTTA Mauro, GABANO Elisabetta, MINASSI Alberto, CAPRIOGLIO Diego CHIM/03, CHIM/06 All 9
1 MS0733 Organic Chemistry TEI Lorenzo, NEGRI Roberto, APPENDINO Giovanni Battista CHIM/06 All NOVARA 6
2 BT068 Functional biochemistry with elements of laboratory BALDANZI Gianluca, CAPELLO Daniela, BERTONI Alessandra BIO/10 All NOVARA 11
2 BT027 Fundamentals of immunology and medical microbiology AZZIMONTI Barbara, DIANZANI Umberto, CHIOCCHETTI Annalisa MED/04, MED/07 All 10
2 MS0443 Genetics GIORDANO Mara, PERSICHETTI Francesca, CORRADO Lucia, BARIZZONE Nadia MED/03 All 6
2 BT035 Human physiology GROSSINI Elena, LIM Dmitry BIO/09 All NOVARA 5
2 BT031 Laboratory cell cultures MERLIN Simone, BOLDORINI Renzo Luciano, BORSOTTI Chiara MED/08, BIO/17 All NOVARA 6
2 BT022 Molecular Biology SANTORO Claudio Ventura, PELICCI Giuliana, CORA' Davide, CORAZZARI Marco BIO/11, BIO/13 All NOVARA 11
3 MS0371 Biotechnological applications in the clinical laboratory ROLLA Roberta, CRISA' Elena MED/15, MED/05 All NOVARA 10
3 MS0450 Clinical Biochemistry and Clinical Molecular Biology CAPELLO Daniela BIO/12 All NOVARA 5
3 BT063 Dissertation Thesis PROFIN_S All NOVARA 10
3 MC117 Elective Didactic Activities NN All NOVARA 12
3 MS0448 Epidemiology BARONE ADESI Francesco, FAGGIANO Fabrizio MED/42 All NOVARA 5
3 MS0120 Further Learning Activities NN All NOVARA 2
3 MS0447 General Pathology CARINI Rita, DIANZANI Irma MED/04 All NOVARA 6
3 MS0449 Pharmaceutical Chemistry PIRALI Tracey, SORBA Giovanni, MASSAROTTI Alberto CHIM/08 All NOVARA 5
3 MS0432 Pharmacology and Innovation management FRESU Luigia Grazia, CONICELLA Fabrizio, SEDDIO Pasquale, JOMMI Claudio SECS-P/08, BIO/14, SECS-P/07 All NOVARA 12
3 MS0446 Statistical methods for experimental studies MAGNANI Corrado MED/01 All NOVARA 5
3 MS0146 Training NN All NOVARA 8
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CourseFundamentals of mathematics and physics
Course IDMS0729
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersTRIVERO Paolo, PANZIERI Daniele, RINALDI Maurizio, GASTALDI Fabio, MARTIGNONE Francesca
CFU12
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year1
PeriodPrimo Semestre
SiteNOVARA
Grading typeFinal grade
Modules
Course ID Course SSD Teachers
MS0730 Physics foundations FIS/01 - Experimental physics PANZIERI Daniele, TRIVERO Paolo
MS0728 Mathematics foundations MAT/04 - Mathematics education and history of mathematics RINALDI Maurizio, GASTALDI Fabio, MARTIGNONE Francesca
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CoursePhysics foundations
Course IDMS0730
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPANZIERI Daniele
TeachersTRIVERO Paolo, PANZIERI Daniele
CFU6
Teaching duration (hours)48
Individual study time 102
SSDFIS/01 - Experimental physics
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year1
Sites and/or partitions
Gruppo A
Gruppo B
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CoursePhysics foundations
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersTRIVERO Paolo, PANZIERI Daniele
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractThe purpose of the course is to provide students the basic knowledge of the fundamental laws of classical physics and some elements of modern physics (applied to optics)
Reference textsSlides explained and shown during the classes and published on the course web page.
Teaching targetsThe course aims to deepen the elements of physics (optics) that may be useful for the degree and to start the students to the methodologies of analysis of experimental data.
PrerequisitesBasic knowledge of mathematics (e.g.: algebra, geometry, elementary calculus).
Didattics MethodsLectures in the classroom with slide presentations.
Other informationsLearning control with examples of closed-answer quizzes on theoretical parts.
Grading rulesThe exam consists of an oral, individual test. The oral test evaluates the student's ability to apply the knowledge acquired during the course. During the written test it is not allowed to consult notes and books. The evaluation is expressed in thirtieths and the test is passed if the score is equal to or greater than 18.
Full argumentsMeasures - scalars and vectors. Kinematics in one and two dimensions. Dynamics, Work and Energy. Rotational Kinematic and Dynamic. Fluids and elasticity. Temperature, Kinetic laws of gases, Thermodynamics laws. Electric field, current in solids, liquids and gases. Electric circuits, Magnetic field,. Electromagnetic waves. Optics and optic instruments. Diffraction and interference.
Expected learning objectivesKnowledge and understanding": Acquisition of a deep knowledge of physical phenomena, knowledge of the concepts and applications of the fundamental laws of classical physics (optics and electromagnetism), acquisition of appropriate scientific language. "Knowledge and understanding of applied skills": knowing how to identify the data necessary to characterize a physical phenomenon; ability to interpret data and understand orders of magnitude, ability to apply the fundamental principles and laws of physics to solve problems and describe natural phenomena. Ability to use the teaching material for a critical and reasoned study.
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CoursePhysics foundations
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersTRIVERO Paolo, PANZIERI Daniele
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractThe purpose of the course is to provide students the basic knowledge of the fundamental laws of classical physics and some elements of modern physics (applied to optics)
Reference textsSlides explained and shown during the classes and published on the course web page.
Teaching targetsThe course aims to deepen the elements of physics (optics) that may be useful for the degree and to start the students to the methodologies of analysis of experimental data.
PrerequisitesBasic knowledge of mathematics (e.g.: algebra, geometry, elementary calculus).
Didattics MethodsLectures in the classroom with slide presentations.
Other informationsLearning control with examples of closed-answer quizzes on theoretical parts.
Grading rulesThe exam consists of a written, individual test. The written test evaluates the student's ability to apply the knowledge acquired during the course (all modules) and consists of 30 closed-answer questions. The level of difficulty of the questions corresponds to the program carried out and to the reference texts indicated on DIR. For each correct answer 1 point is assigned. During the written test it is not allowed to consult notes and books. The evaluation is expressed in thirtieths and the test is passed if the score is equal to or greater than 18.
Full argumentsMeasures - scalars and vectors. Kinematics in one and two dimensions. Dynamics, Work and Energy. Rotational Kinematic and Dynamic. Fluids and elasticity. Temperature, Kinetic laws of gases, Thermodynamics laws. Electric field, current in solids, liquids and gases. Electric circuits, Magnetic field,. Electromagnetic waves. Optics and optic instruments. Diffraction and interference.
Expected learning objectivesKnowledge and understanding": Acquisition of a deep knowledge of physical phenomena, knowledge of the concepts and applications of the fundamental laws of classical physics (optics and electromagnetism), acquisition of appropriate scientific language. "Knowledge and understanding of applied skills": knowing how to identify the data necessary to characterize a physical phenomenon; ability to interpret data and understand orders of magnitude, ability to apply the fundamental principles and laws of physics to solve problems and describe natural phenomena. Ability to use the teaching material for a critical and reasoned study.
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CourseMathematics foundations
Course IDMS0728
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderRINALDI Maurizio
TeachersRINALDI Maurizio, GASTALDI Fabio, MARTIGNONE Francesca
CFU6
Teaching duration (hours)48
Individual study time 102
SSDMAT/04 - Mathematics education and history of mathematics
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year1
Sites and/or partitions
Gruppo A
Gruppo B
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CourseMathematics foundations
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersRINALDI Maurizio
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractBasic mathematics, functions, derivatives, integrals. Some elements of descriptive statistics.
Reference textsSergio Invernizzi, Maurizio Rinaldi, Andrea Sgarro, Moduli di Matematica e Statistica, Zanichelli Editore, Bologna 2000
Teaching targets*Knowledge and understanding. The course aims at providing student with the knowledge and the ability to manage the mathematical tools required to understand and follow the other courses of the Degree and at introducing her/him to the numerical and graphical presentation and synthesis of simple series of experimental data and to the modeling of natural phenomena. More specifically the course aims at providing students with basic methods of differential and integral calculus needed to understand mathematical models. *Applying knowledge and understanding Students are expected to be able to use the acquired skills even in a multidisciplinary context and they should be able to resort to mathematics and statistics even in situations external to the course. *Making judgements. At the end of the course students are expected to apply learned methods even in different situations and that they have acquired the tools needed to extend their knowledge by themselves. *Communication skills. At the end of the course students are expected to be able to express the learned concepts in a clear way. *Learning skills. During the course students should learn how to study by choosing their personal path and should become able to choose the appropriate resources, possibly online.
PrerequisitesThe student should have the basic knowledge of the subject that can be acquired in a normal high school. In particular: arithmetic operations, powers, Cartesian plane, equation of the line, parabola and circumference, angles, measured in degrees and radians, circular functions, fundamental formulas of plane and solid geometry.
Didattics MethodsLectures are given in the classroom.
Other informationsReference material and further details are provided on the DIR website [https://www.dir.uniupo.it/ ] . Enrolment key is provided during the lectures.
Grading rulesOngoing Quizzes and online Final Examination. The ongoing quizzes are assigned periodically at the end of a given subject. The Final focuses on all the course matters. Final evaluation gives a weight of 20% to the ongoing quizzes and 80% to the Final. Ongoing quizzes could be replaced by an oral examination
Full arguments0.1 Numbers 0.2. Equation and inequalities 0.3. The Cartesian plane. Straight lines and simple curves. Linear systems. 0.4 Angles and their measures: radian. 1.1. Concept of mathematical functions. Domain and codomain. Image. Function composition. Invertible function and inverse function. 1.2. Cosine, Sine and tangent and their inverse functions. Logarithms and exponential functions. 2.1 Geometric meaning of the Derivative. Graphic computation of the derivative through repeated zooms. Approximation of the derivative at a point via Newton quotient. Three point rule. Derivative for tabulated functions. Derivative function. 2.2 Differentiation rules Derivative of the sum, of the product, of the composite function, of the reciprocal and of the quotient. Derivative of the inverse function. Applications of the derivatives 2.3 Increasing and decreasing functions, Minima and maxima of functions. Computation of maxima and minima with the help of the derivative. Second derivative and study of concavity-convexity of a graph. Netwon’s method. 3.1 Numerical Integration Definite integral for positive functions over finite intervals, Computation. 3.2. Numerical integration with the rectangle methods and trapezoid method. Stochastic integration with the Montecarlo methods. Exact integration The Fundamental Theorem of Calculus. Indefinite integral and antiderivatives. Computation of antiderivatives. 3.3. Area between two curves. Extensions of the Integral Integrals for non positive functions. Inversions of integration endpoints. 4.1. Basic elements of Statistics. Statistical data and their representations. Population and samples. Variables and observations. Graphical representations: bar charts, histograms and box-plot. Numerical representations and statistical indices. Scatter plot. Linear regression. Variable transformations to reduce to a linear relationship.
Expected learning objectivesKnowledge of some basic concepts and methods of mathematics, in particular: real numbers and their representations, main elementary functions, derivative and its geometric interpretation, simple integrals, calculation of areas and basic elements of Statistics. Ability at applying these concepts and methods in the modelisation of simple problems in which there are numerical and graphical presentations and synthesis of simple series of experimental data. Ability at communicating problem solutions in a clear and complete fashion. Ability in choosing the mathematical resources useful to deal with simple problems where it is required to interpret and use modeling of natural phenomena.
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CourseMathematics foundations
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersGASTALDI Fabio, MARTIGNONE Francesca
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractBasic mathematics, functions, derivatives, integrals. Some elements of descriptive statistics.
Reference textsSergio Invernizzi, Maurizio Rinaldi, Andrea Sgarro, Moduli di Matematica e Statistica, Zanichelli Editore, Bologna 2000
Teaching targets*Knowledge and understanding. The course aims at providing student with the knowledge and the ability to manage the mathematical tools required to understand and follow the other courses of the Degree and at introducing her/him to the numerical and graphical presentation and synthesis of simple series of experimental data and to the modeling of natural phenomena. More specifically the course aims at providing students with basic methods of differential and integral calculus needed to understand mathematical models. *Applying knowledge and understanding Students are expected to be able to use the acquired skills even in a multidisciplinary context and they should be able to resort to mathematics and statistics even in situations external to the course. *Making judgements. At the end of the course students are expected to apply learned methods even in different situations and that they have acquired the tools needed to extend their knowledge by themselves. *Communication skills. At the end of the course students are expected to be able to express the learned concepts in a clear way. *Learning skills. During the course students should learn how to study by choosing their personal path and should become able to choose the appropriate resources, possibly online.
PrerequisitesThe student should have the basic knowledge of the subject that can be acquired in a normal high school. In particular: arithmetic operations, powers, Cartesian plane, equation of the line, parabola and circumference, angles, measured in degrees and radians, circular functions, fundamental formulas of plane and solid geometry.
Didattics MethodsLectures are given in the classroom.
Other informationsReference material and further details are provided on the DIR website [https://www.dir.uniupo.it/ ] . Enrolment key is provided during the lectures.
Grading rulesOngoing Quizzes and online Final Examination. The ongoing quizzes are assigned periodically at the end of a given subject. The Final focuses on all the course matters. Final evaluation gives a weight of 20% to the ongoing quizzes and 80% to the Final. Ongoing quizzes could be replaced by an oral examination
Full arguments0.1 Numbers 0.2. Equation and inequalities 0.3. The Cartesian plane. Straight lines and simple curves. Linear systems. 0.4 Angles and their measures: radian. 1.1. Concept of mathematical functions. Domain and codomain. Image. Function composition. Invertible function and inverse function. 1.2. Cosine, Sine and tangent and their inverse functions. Logarithms and exponential functions. 2.1 Geometric meaning of the Derivative. Graphic computation of the derivative through repeated zooms. Approximation of the derivative at a point via Newton quotient. Three point rule. Derivative for tabulated functions. Derivative function. 2.2 Differentiation rules Derivative of the sum, of the product, of the composite function, of the reciprocal and of the quotient. Derivative of the inverse function. Applications of the derivatives 2.3 Increasing and decreasing functions, Minima and maxima of functions. Computation of maxima and minima with the help of the derivative. Second derivative and study of concavity-convexity of a graph. Netwon’s method. 3.1 Numerical Integration Definite integral for positive functions over finite intervals, Computation. 3.2. Numerical integration with the rectangle methods and trapezoid method. Stochastic integration with the Montecarlo methods. Exact integration The Fundamental Theorem of Calculus. Indefinite integral and antiderivatives. Computation of antiderivatives. 3.3. Area between two curves. Extensions of the Integral Integrals for non positive functions. Inversions of integration endpoints. 4.1. Basic elements of Statistics. Statistical data and their representations. Population and samples. Variables and observations. Graphical representations: bar charts, histograms and box-plot. Numerical representations and statistical indices. Scatter plot. Linear regression. Variable transformations to reduce to a linear relationship.
Expected learning objectivesKnowledge of some basic concepts and methods of mathematics, in particular: real numbers and their representations, main elementary functions, derivative and its geometric interpretation, simple integrals, calculation of areas and basic elements of Statistics. Ability at applying these concepts and methods in the modelisation of simple problems in which there are numerical and graphical presentations and synthesis of simple series of experimental data. Ability at communicating problem solutions in a clear and complete fashion. Ability in choosing the mathematical resources useful to deal with simple problems where it is required to interpret and use modeling of natural phenomena.
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CourseGenetics
Course IDMS0734
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersSOLURI Angela Maria Felicia, PERSICHETTI Francesca, GIORDANO Mara, CORRADO Lucia
CFU9
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year1
PeriodSecondo Semestre
SiteNOVARA
Grading typeFinal grade
Modules
Course ID Course SSD Teachers
MS0735 Genetics of microorganisms and eukaryotic BIO/18 - Genetics PERSICHETTI Francesca, SOLURI Angela Maria Felicia
MS0736 Human Genetics MED/03 - Medical genetics GIORDANO Mara, CORRADO Lucia
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CourseGenetics of microorganisms and eukaryotic
Course IDMS0735
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPERSICHETTI Francesca
TeachersSOLURI Angela Maria Felicia, PERSICHETTI Francesca
CFU4
Teaching duration (hours)32
Individual study time 68
SSDBIO/18 - Genetics
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year1
Sites and/or partitions
Gruppo A
Gruppo B
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CourseGenetics of microorganisms and eukaryotic
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderSOLURI Angela Maria Felicia
TeachersSOLURI Angela Maria Felicia
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageITALIAN
AbstractThe course provides knowledge on the structure, organization, replication and transmission of the hereditary material in prokaryotes and eukaryotes.
Reference textsRUSSEL P.J. Genetica “un approccio molecolare”, Ed. Pearson SNUSTAD P.D., SIMMONS M.J.- Principi di genetica, Ed. EdiSES. GRIFFITHS A.J.F., GELBART W., LEWONTIN R.C., SUZUKI D.T., MILLER J.H., WESSLER S.R. Genetica - Principi di analisi formale, Ed. Zanichelli.
Teaching targetsThe course aims to teach the student the organization, expression and transmission of genetic material, both in prokaryotes and eukaryotes. Student will be provided of the tools to understand and recognize the different mode of transmission of genetic traits, with particular emphasis to the Mendelian inheritance and its extensions.
PrerequisitesBasic knowledge of biology.
Didattics MethodsClass lectures with PowerPoint projections and use of the overhead projector for exercises explanation and solution.
Other informationsLearning control: In-class writing exercises
Grading rulesWritten test. The test consists of 31 multiple choice questions (5 options, only one correct) both theoretical on the different topics of the program, and simple problems of formal genetics. The questions will receive a score of 1 if the answer is correct and zero if the answer is incorrect or void. The minimum score to pass the exam is 18 points. The honors will be awarded to students who receive a total score of more than 30.
Full argumentsThe genetic material: chemical composition and structure. Replication, transcription and translation of genetic material. DNA organization in prokaryotic and eukaryotic chromosomes. Mutation and DNA repair. Cell division: mitosis and meiosis. Mendel's laws and their applications. Extension of mendelian analysis: multiple alleles and genetic interaction (epistasis, penetrance and expressivity). Genetic recombination. Genetics of microorganisms: bacteria, bacteriophages and yeast. Exchange of genetic material: conjugation, transformation and transduction. Regulation of gene expression in bacteria and bacteriophages.
Expected learning objectivesKnowledge and understanding To know the structure, replication and transmission of the genetic material. To understand the bases of the Mendelian inheritance and of the interaction among genes. To know the most important mechanisms of transferring of genetic material and regulation of gene expression in prokaryotes. Applying knowledge and understanding: At the end of the course the student should be able: To apply the acquired knowledge to solve questions regarding the Mendelian inheritance, both qualitatively (expected genotypes and phenotypes) and quantitatively (expected frequencies). To distinguish among the different types of inheritance and to predict the phenotypic effects of the interactions between alleles at a single locus and among genes. To describe and compare the different mode of transferring genetic information between bacteria. Making judgements Ability to formulate hypotheses about genetic transmission and to critically discuss the complex relationships between genotype and phenotype. Communication skills Ability to expose the subjects of the course in a clear and comprehensible way and with the use of the appropriate scientific terminology. Learning skills Ability to deepen their knowledge of the genetics on several books and on scientific articles in order to follow with a critical attitude the advancement of discipline.
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CourseGenetics of microorganisms and eukaryotic
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPERSICHETTI Francesca
TeachersPERSICHETTI Francesca
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractThe course provides knowledge on the structure, organization, replication and transmission of the hereditary material in prokaryotes and eukaryotes.
Reference textsRUSSEL P.J. Genetica “un approccio molecolare”, Ed. Pearson SNUSTAD P.D., SIMMONS M.J.- Principi di genetica, Ed. EdiSES. GRIFFITHS A.J.F., GELBART W., LEWONTIN R.C., SUZUKI D.T., MILLER J.H., WESSLER S.R. Genetica - Principi di analisi formale, Ed. Zanichelli.
Teaching targetsThe course aims to teach the student the organization, expression and transmission of genetic material, both in prokaryotes and eukaryotes. Student will be provided of the tools to understand and recognize the different mode of transmission of genetic traits, with particular emphasis to the Mendelian inheritance and its extensions.
PrerequisitesBasic knowledge of biology.
Didattics MethodsClass lectures with PowerPoint projections and use of the overhead projector for exercises explanation and solution.
Other informationsLearning control: In-class writing exercises
Grading rulesWritten test. The test consists of 31 multiple choice questions (5 options, only one correct) both theoretical on the different topics of the program, and simple problems of formal genetics. The questions will receive a score of 1 if the answer is correct and zero if the answer is incorrect or void. The minimum score to pass the exam is 18 points. The honors will be awarded to students who receive a total score of more than 30.
Full argumentsThe genetic material: chemical composition and structure. Replication, transcription and translation of genetic material. DNA organization in prokaryotic and eukaryotic chromosomes. Mutation and DNA repair. Cell division: mitosis and meiosis. Mendel's laws and their applications. Extension of mendelian analysis: multiple alleles and genetic interaction (epistasis, penetrance and expressivity). Genetic recombination. Genetics of microorganisms: bacteria, bacteriophages and yeast. Exchange of genetic material: conjugation, transformation and transduction. Regulation of gene expression in bacteria and bacteriophages.
Expected learning objectivesKnowledge and understanding To know the structure, replication and transmission of the genetic material. To understand the bases of the Mendelian inheritance and of the interaction among genes. To know the most important mechanisms of transferring of genetic material and regulation of gene expression in prokaryotes. Applying knowledge and understanding: At the end of the course the student should be able: To apply the acquired knowledge to solve questions regarding the Mendelian inheritance, both qualitatively (expected genotypes and phenotypes) and quantitatively (expected frequencies). To distinguish among the different types of inheritance and to predict the phenotypic effects of the interactions between alleles at a single locus and among genes. To describe and compare the different mode of transferring genetic information between bacteria. Making judgements Ability to formulate hypotheses about genetic transmission and to critically discuss the complex relationships between genotype and phenotype. Communication skills Ability to expose the subjects of the course in a clear and comprehensible way and with the use of the appropriate scientific terminology. Learning skills Ability to deepen their knowledge of the genetics on several books and on scientific articles in order to follow with a critical attitude the advancement of discipline.
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CourseHuman Genetics
Course IDMS0736
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGIORDANO Mara
TeachersGIORDANO Mara, CORRADO Lucia
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/03 - Medical genetics
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year1
Sites and/or partitions
Gruppo A
Gruppo B
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CourseHuman Genetics
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersGIORDANO Mara
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractStructure and evolution of the human genome. - Cell division: mitosis and meiosis. -Correlation genotype-phenotype (example: ABO blood group, sickle-cell anemia). -Principles of Mendelian genetics. - Exceptions to the laws of Mendel. - Chromosomal classification criteria and chromosomal aberrations - Population genetics. -Transmission of independent and concatenated characters. Linkage analysis. - Quantitative and semi-quantitative multifactorial traits. - Methods of molecular genetics - Analysis of genomic databases
Reference textsEredità, Principi e problematiche della genetica umana, M.R. Cummings, Edizioni EdiSES, II edizione (2009) Genetica, Peter J. Russel, Edizioni EdiSES, II edizione Genetica Umana e Medica Edizioni EDRA Masson 2014 (III edizione) Giovanni Neri e Maurizio Genuardi
Teaching targetsTo understand the relationships between genetic information and the phenotype. To Know how to evaluate the risk of recurrence of genetic diseases or with genetic components in families. To know the molecular basis of genetically based diseases. Apply knowledge to the analysis and resolution of problems of formal and molecular genetics. To apply the basic methods of molecular genetics such as PCR and sequencing through practical laboratory activities
PrerequisitesFundamentals of biochemistry, cell biology and statistics.
Didattics MethodsLectures with presentations in MS-Power Point format.
Grading rulesThe knowledge will be verified by written exam with open and / or multiple choice questions. For most questions, the answer will require the solving of problems
Full argumentsDNA structure. Organization of DNA in chromosomes. Structure and function of the chromosome. -The Human Genome structure. Organization of genes. Transposable elements. Ripetitive DNA sequences. Gene families. -Cell division: mitosis and meiosis. -Genotype-phenotype correlation (example: ABO blood group, sickle cell anemia). Protein translation and genetic code. Correlation between type of mutations and dominant or recessive phenotype at different levels of phenotype investigation. Genetic consequences of meiosis. -Principles of Mendelian genetics. Transmission in families of autosomal recessive, dominant and X-linked monofactorial traits; recurrence risks in families. -"Exceptions" to Mendel’s rules. Incomplete penetrance, variable expressivity, genetic heterogeneity. X-chromosome inactivation, mitochondrial inheritance, genomic imprinting -Criteria for classification of chromosomes and methods for their identification. Chromosomal aberrations and their incidence at birth; Reproductive risks associated with chromosomal aberrations. -Population genetics. Gene and genotypic frequencies in the population and Hardy-Weinberg equilibrium. -Evolution of human populations. Evolutionary forces (mutation, selection, genetic drift, migration) that influence allelic and genotype frequencies. -Transmission of independent and concatenated characters. Localization of genes on chromosomes based on their transmission in families (linkage analysis). Different methods for gene mapping. -Multi-factorial characters. Population distribution of multifactorial characters; evaluation of the weight of the genetic component in multifactorial disorders; identification of susceptibility genes in multifactorial diseases. -DNA investigation methods. Polymerase Chain Reaction (PCR); Analysis of VNTR, STR and fingerprint polymorphisms; DNA sequencing; Methods for the identification of nucleotide variations. Genomic analysis through next generation sequencing methods and chromosomal microarray (MCA). -Analysis of Genomic Databases
Expected learning objectivesAt the end of the course, students should be able to: -Understand the relationships between genetic information and the phenotype. - Know how to evaluate the risk of recurrence of genetic diseases or with genetic components in families. -Know the molecular basis of genetically based diseases. -Apply knowledge to the analysis and resolution of problems of formal and molecular genetics.
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CourseHuman Genetics
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersCORRADO Lucia
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractStructure and evolution of the human genome. - Cell division: mitosis and meiosis. -Correlation genotype-phenotype (example: ABO blood group, sickle-cell anemia). -Principles of Mendelian genetics. - Exceptions to the laws of Mendel. - Chromosomal classification criteria and chromosomal aberrations - Population genetics. -Transmission of independent and concatenated characters. Linkage analysis. - Quantitative and semi-quantitative multifactorial traits. - Methods of molecular genetics - Analysis of genomic databases
Reference textsEredità, Principi e problematiche della genetica umana, M.R. Cummings, Edizioni EdiSES, II edizione (2009) Genetica, Peter J. Russel, Edizioni EdiSES, II edizione Genetica Umana e Medica Edizioni EDRA Masson 2014 (III edizione) Giovanni Neri e Maurizio Genuardi
Teaching targetsTo understand the relationships between genetic information and the phenotype. To Know how to evaluate the risk of recurrence of genetic diseases or with genetic components in families. To know the molecular basis of genetically based diseases. Apply knowledge to the analysis and resolution of problems of formal and molecular genetics. To apply the basic methods of molecular genetics such as PCR and sequencing through practical laboratory activities
PrerequisitesFundamentals of biochemistry, cell biology and statistics.
Didattics MethodsLectures with presentations in MS-Power Point format.
Grading rulesThe knowledge will be verified by written exam with open and / or multiple choice questions. For most questions, the answer will require the solving of problems
Full argumentsDNA structure. Organization of DNA in chromosomes. Structure and function of the chromosome. -The Human Genome structure. Organization of genes. Transposable elements. Ripetitive DNA sequences. Gene families. -Cell division: mitosis and meiosis. -Genotype-phenotype correlation (example: ABO blood group, sickle cell anemia). Protein translation and genetic code. Correlation between type of mutations and dominant or recessive phenotype at different levels of phenotype investigation. Genetic consequences of meiosis. -Principles of Mendelian genetics. Transmission in families of autosomal recessive, dominant and X-linked monofactorial traits; recurrence risks in families. -"Exceptions" to Mendel’s rules. Incomplete penetrance, variable expressivity, genetic heterogeneity. X-chromosome inactivation, mitochondrial inheritance, genomic imprinting -Criteria for classification of chromosomes and methods for their identification. Chromosomal aberrations and their incidence at birth; Reproductive risks associated with chromosomal aberrations. -Population genetics. Gene and genotypic frequencies in the population and Hardy-Weinberg equilibrium. -Evolution of human populations. Evolutionary forces (mutation, selection, genetic drift, migration) that influence allelic and genotype frequencies. -Transmission of independent and concatenated characters. Localization of genes on chromosomes based on their transmission in families (linkage analysis). Different methods for gene mapping. -Multi-factorial characters. Population distribution of multifactorial characters; evaluation of the weight of the genetic component in multifactorial disorders; identification of susceptibility genes in multifactorial diseases. -DNA investigation methods. Polymerase Chain Reaction (PCR); Analysis of VNTR, STR and fingerprint polymorphisms; DNA sequencing; Methods for the identification of nucleotide variations. Genomic analysis through next generation sequencing methods and chromosomal microarray (MCA). -Analysis of Genomic Databases
Expected learning objectivesAt the end of the course, students should be able to: -Understand the relationships between genetic information and the phenotype. - Know how to evaluate the risk of recurrence of genetic diseases or with genetic components in families. -Know the molecular basis of genetically based diseases. -Apply knowledge to the analysis and resolution of problems of formal and molecular genetics.
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CourseHistology and anatomy
Course IDMS0731
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPRAT Maria Giovanna
TeachersCOTELLA Diego, BOZZO Chiarella, PRAT Maria Giovanna, FOLLENZI Antonia, BOCCAFOSCHI Francesca, SABBATINI Maurizio
CFU15
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year1
PeriodAnnuale
SiteNOVARA
Grading typeFinal grade
Teaching languageItalian
AbstractStudy of the characteristics of eukaryotic cells (Biology of the Eukariotic Cells), of the tissues (Histology), and of the organs and systems of the human body (Anatomy). Study of the first periods of the development of the human being, of the mechanisms of their origin in the embryo and the interaction of the tissues to form organs. Stem cells from the beginning in the embryo to the adult. Animal models used to study developmental biology.
Reference textsAlberts, Bray, Hopkin, Johnson, Lewis et al BIOLOGIA MOLECOLARE DELLA CELLULA (l'essenziale) ed. Zanichelli Ginelli, Malcovati Molecole, Cellule e Organismi. ed. EdiSES Wheater: Istologia e Anatomia microscopica, CEA Stevens-Lowe: Istologia Umana, CEA Barbieri-Carinci: Embriologia, III ed. CEA Saladin: Anatomia Umana Casa editrice PICCIN
Teaching targetsthe end of the course the student must: - have acquired the basic knowledge on the structure and organization of the eukariotc cell, the molecular and cellular components of the tissues, their organization in organs and systems. Moreover, of the embryological origin of the different tissues of the human body - have acquired the knowledge about tissue and organ homeostasis - have acquired basic knowledge on the first steps of the embryonic development (the first two months in humans) and of some animal models used for the study of embryonic development
PrerequisitesThe student must be in possession of biology and genetics concepts, such as those offered at the high school.
Didattics MethodsDirect lessons in classroom with the use of files in Presentation format and videos.
Other informationsVideo projector and computers with Internet connection supplied to the classroom
Grading rulesThe exam consists of a multichoise written proof . Registration is mandatory. Only correct answers are considered for the final vote.
Full argumentsThe program is referred in the single three courses
Expected learning objectivesIn order to reach the knowledge and skills corresponding to the minimum level of sufficiency, the student is asked to demonstrate: (KNOWLEDGE) -to know the structure, organization and function of the eukaryotic cell; -to understand the molecular mechanisms that govern the flow of gene information from DNA to proteins;to know the structure of the cells and extracellular matrix organization and their role in the formation of the different tissues; the organization of the latter in the different organs and systems/apparatus; to know the macroscopic anatomy of the human body. (SKILLS) Ability to understand the basics of cellular and tissue testing and methods for their study. (Additional SKILLS) - to be able to read, understand and comment scientific concepts from textbooks or specialized literature - to know, or to be able to trace, the essential terminology. - to be able to evaluate the didactics delivered
Modules
Course ID Course SSD Teachers
MS0002 Eukaryotic cell biology BIO/13 - Experimental biology COTELLA Diego, BOZZO Chiarella
MS0732 Histology and developmental biology BIO/17 - Histology PRAT Maria Giovanna, FOLLENZI Antonia
BT015 Human Anatomy BIO/16 - Human anatomy BOCCAFOSCHI Francesca, SABBATINI Maurizio
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CourseEukaryotic cell biology
Course IDMS0002
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersCOTELLA Diego, BOZZO Chiarella
CFU5
Teaching duration (hours)40
Individual study time 85
SSDBIO/13 - Experimental biology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year1
PeriodPrimo Semestre
Sites and/or partitions
Gruppo A
Gruppo B
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CourseEukaryotic cell biology
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCOTELLA Diego
TeachersCOTELLA Diego
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractThis course deals with the biology of the eukaryotic cell: structure, function and biosynthesis of cell membranes and organelles; the cytoskeleton and the extracellular matrix. Chromatin structure, the synthesis of RNA and protein and gene flow of information.
Reference textsAlberts, Bray, Hopkin, Johnson, Lewis et al BIOLOGIA MOLECOLARE DELLA CELLULA (l'essenziale) ed. Zanichelli Ginelli, Malcovati Molecole, Cellule e Organismi. ed. EdiSES
Teaching targetsThe module will provide students with the basic knowledge on the structure, organization and function of the eukaryotic cell. Attention will be given to the understanding of the molecular mechanisms that regulate cell behavior and their biotechnological application. The module will also address the cellular and post-genomic methods that have enabled it to acquire detailed information on cell function.
PrerequisitesThe student must be in possession of biology and genetics concepts, such as those offered at the high school.
Didattics MethodsPresentations in MS-Powerpoint format.
Other informationsVideo projector and computers with Internet connection supplied to the classroom
Grading rulesThe exam consists of a written test with multiple choice questions.
Full argumentsStructure and organization of the cell • Definition life • The eukaryotic cell and prokaryotic cell • The cellular compartments. • The lipids as constituents of chemical barriers Biological membranes • The lipids of biological membranes: phospholipids, glycolipids and cholesterol • The organization dual layer or in micelle • The fluidity of the phospholipid bilayer: the role of unsaturated fats • The intrinsic and extrinsic proteins • The theory of fluid Mosaic The transport mechanisms through membranes • the lipid bilayer permeability • simple and facilitated diffusion • Passive transport and active transport • channel proteins and carrier proteins: kinetic properties of the two systems and operating methods • Glucose Transporter • Pump Na + / K + ATPase: operating mode • Antiport and Simport • ligand-activated channels and channels activated by voltage • The membrane potential and the action potential Mitochondria and ATP synthesis • General knowledge on the process of glycolysis, oxidation of pyruvate, Krebs cycle and the electron transport chain • The structure of the mitochondrion • The mitochondrial genome • The evolutionary origin of mitochondria • The transport of proteins in mitochondria The endoplasmic reticulum and Golgi apparatus • Summary of the membrane proteins and secretion • Glycosylation and maturation of proteins in the Golgi • vesicular traffic: vesicle formation, vesicle movement, recognition of the target organelle • Exocytosis addressing mechanisms of proteins in cellular compartments • signal sequences and protein localization (entry into the nucleus, the endoplasmic reticulum, the mitochondria) endocytosis • pinocytosis, phagocytosis and receptor-mediated endocytosis Degradation of biological macromolecules in the cell • The lysosome • The proteasome The peroxisomes the cytoskeleton • Microfilaments contractile actin and myosin and cell movement • intermediate filaments • Microtubules and organelle movement • Muscle contraction The extracellular matrix • The collagen, laminin the fibronectin, proteoglycans • The extracellular matrix proteins establish multiple interactions • The matrix-cell interaction is mediated by specific receptors: the integrins • Structure and function of integrins and cadherins The cell cycle and mitosis • The cell cycle G1, S, G2, and M. • The role of cyclins and cdk in cell cycle progression • The check point of cell cycle checkpoint in G1 (Rb / E2F), G2 (p53) and mitotic checkpoint (Cdc20 / APC). Cell death and apoptosis • necrosis • Apoptosis: the intrinsic pathway (p53 and mitochondria) and extrinsic (the death receptor) Gene flow of information: the definition of gene era ENCODE • DNA replication and maintenance of network integrity • DNA Transcription: synthesis of RNA and their maturation • Influence of environment on gene flow: Epigenetics • The RNA interference
Expected learning objectivesIn order to reach the knowledge and skills corresponding to the minimum level of sufficiency, the student is asked to demonstrate: (KNOWLEDGE) - to know the structure, organization and function of the eukaryotic cell; - to understand the molecular mechanisms that govern the flow of gene information from DNA to proteins. (SKILLS) Ability to understand the basics of cellular and tissue testing and methods for their study. (Additional SKILLS) - to be able to read, understand and comment scientific concepts from textbooks or specialized literature - to know, or to be able to trace, the essential terminology. - to be able to evaluate the didactics delivered
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CourseEukaryotic cell biology
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBOZZO Chiarella
TeachersBOZZO Chiarella
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractProgramma dettagliato del corso di Biologia della Cellula Eucariotica (MS002) A partire da A.A. 2017-18 Docente del corso: Dr.ssa Chiarella Bozzo Corso di laurea in Biotecnologie Struttura ed organizzazione della cellula • Definizione di essere vivente • La cellula eucariotica e la cellula procariotica • I compartimenti cellulari. • I lipidi come costituenti di barriere chimiche Le membrane biologiche • I lipidi delle membrane biologiche: fosfolipidi, glicolipidi e colesterolo • L'organizzazione a doppio strato o a micella • La fluidità' del doppio strato fosfolipidico: ruolo dei grassi insaturi • Le proteine intrinseche e proteine estrinseche • La teoria del Mosaico fluido I meccanismi di trasporto attraverso le membrane • Caratteristiche di permeabilità del doppio strato lipidico • Diffusione semplice e diffusione facilitata • Trasporto passivo e trasporto attivo • Proteine canale e proteine trasportatrici: caratteristiche cinetiche dei due sistemi e modalità di funzionamento • Trasportatore del glucosio • Pompa Na+/K+ ATPasi: modalità di funzionamento • Antiporto e simporto • Canali attivati da ligando e canali attivati da voltaggio • ll potenziale di membrana e il potenziale d’azione Mitocondri e la sintesi di ATP • Nozioni generali sul processo di glicolisi, ossidazione del piruvato, ciclo di Krebs e catena di trasporto degli elettroni • La struttura del mitocondrio • Il genoma mitocondriale • L’origine evolutiva del mitocondrio • Il trasporto delle proteine nel mitocondrio Reticolo endoplasmatico rugoso e apparato di Golgi • Sintesi delle proteine di membrana e di secrezione • Glicosilazione e maturazione delle proteine nel Golgi • Traffico vescicolare: formazione di vescicole, movimento delle vescicole, riconoscimento del organello bersaglio • Esocitosi Meccanismi di indirizzamento delle proteine nei compartimenti cellulari • Sequenze segnale e localizzazione delle proteine (entrata nel nucleo, nel reticolo endoplasmatico, nel mitocondrio) Endocitosi • Pinocitosi, fagocitosi e endocitosi mediata da recettore Degradazione delle macromolecole biologiche nella cellula • Il lisosoma • Il proteosoma I perossisomi Il citoscheletro • Microfilamenti contrattili di actina e miosina e il movimento cellulare • Filamenti intermedi • Microtubuli ed il movimento degli organelli • La contrazione muscolare La matrice extracellulare • I collageni, laminine le fibronectine, i proteoglicani • Le proteine della matrice extracellulare stabiliscono interazioni multiple • L'interazione matrice-cellula è mediata da recettori specifici: le integrine • Struttura e funzione delle integrine e delle caderine Il ciclo cellulare e la mitosi • Il ciclo cellulare G1, S, G2 ed M. • Il ruolo delle cicline e delle cdk nella progressione del ciclo cellulare • I check point del ciclo cellulare: check point di G1 (Rb/E2F), di G2 (p53) e check point mitotico (cdc20/APC). Morte cellulare e apoptosi • Necrosi • Apoptosi: la via intrinseca (p53 e il mitocondrio) e la via estrinseca (i recettori di morte) Flusso dell’informazione genica: la definizione di gene nell’era ENCODE • Replicazione del DNA e mantenimento della sua integrità • Trascrizione del DNA: sintesi degli RNA e loro maturazione • Influenza dell’ambiente sul flusso genico: Epigenetica • Stabilità degli RNA: l’interferenza
Reference textsAlberts, Bray, Hopkin, Johnson, Lewis et al BIOLOGIA MOLECOLARE DELLA CELLULA (l'essenziale) ed. Zanichelli Ginelli, Malcovati MOLECOLE, CELLULE E ORGANISMI ed. EdiSES Karp BIOLOGIA CELLULARE E MOLECOLARE ed. EDISES Cooper, Hausman LA CELLULA - UN APPROCCIO MOLECOLARE ed. Piccin
Teaching targetsIl modulo ha l’obiettivo di fornire le nozioni essenziali di biologia cellulare e molecolare che forniranno allo studente gli strumenti necessari per arrivare a conoscere e descrivere in modo organico la struttura di cellule eucariotiche e le loro funzioni.
PrerequisitesLo studente deve essere in possesso delle nozioni basilari di biologia e chimica.
Didattics MethodsLezioni frontali. Presentazioni in formato MS-Power Point, siti internet didattici.
Grading rulesProva scritta con domande a scelta multipla.
Full argumentsProgramma dettagliato del corso di Biologia della Cellula Eucariotica (MS002) A partire da A.A. 2017-18 Docente del corso: Dr.ssa Chiarella Bozzo Corso di laurea in Biotecnologie Struttura ed organizzazione della cellula • Definizione di essere vivente • La cellula eucariotica e la cellula procariotica • I compartimenti cellulari. • I lipidi come costituenti di barriere chimiche Le membrane biologiche • I lipidi delle membrane biologiche: fosfolipidi, glicolipidi e colesterolo • L'organizzazione a doppio strato o a micella • La fluidità' del doppio strato fosfolipidico: ruolo dei grassi insaturi • Le proteine intrinseche e proteine estrinseche • La teoria del Mosaico fluido I meccanismi di trasporto attraverso le membrane • Caratteristiche di permeabilità del doppio strato lipidico • Diffusione semplice e diffusione facilitata • Trasporto passivo e trasporto attivo • Proteine canale e proteine trasportatrici: caratteristiche cinetiche dei due sistemi e modalità di funzionamento • Trasportatore del glucosio • Pompa Na+/K+ ATPasi: modalità di funzionamento • Antiporto e simporto • Canali attivati da ligando e canali attivati da voltaggio • ll potenziale di membrana e il potenziale d’azione Mitocondri e la sintesi di ATP • Nozioni generali sul processo di glicolisi, ossidazione del piruvato, ciclo di Krebs e catena di trasporto degli elettroni • La struttura del mitocondrio • Il genoma mitocondriale • L’origine evolutiva del mitocondrio • Il trasporto delle proteine nel mitocondrio Reticolo endoplasmatico rugoso e apparato di Golgi • Sintesi delle proteine di membrana e di secrezione • Glicosilazione e maturazione delle proteine nel Golgi • Traffico vescicolare: formazione di vescicole, movimento delle vescicole, riconoscimento del organello bersaglio • Esocitosi Meccanismi di indirizzamento delle proteine nei compartimenti cellulari • Sequenze segnale e localizzazione delle proteine (entrata nel nucleo, nel reticolo endoplasmatico, nel mitocondrio) Endocitosi • Pinocitosi, fagocitosi e endocitosi mediata da recettore Degradazione delle macromolecole biologiche nella cellula • Il lisosoma • Il proteosoma I perossisomi Il citoscheletro • Microfilamenti contrattili di actina e miosina e il movimento cellulare • Filamenti intermedi • Microtubuli ed il movimento degli organelli • La contrazione muscolare La matrice extracellulare • I collageni, laminine le fibronectine, i proteoglicani • Le proteine della matrice extracellulare stabiliscono interazioni multiple • L'interazione matrice-cellula è mediata da recettori specifici: le integrine • Struttura e funzione delle integrine e delle caderine Il ciclo cellulare e la mitosi • Il ciclo cellulare G1, S, G2 ed M. • Il ruolo delle cicline e delle cdk nella progressione del ciclo cellulare • I check point del ciclo cellulare: check point di G1 (Rb/E2F), di G2 (p53) e check point mitotico (cdc20/APC). Morte cellulare e apoptosi • Necrosi • Apoptosi: la via intrinseca (p53 e il mitocondrio) e la via estrinseca (i recettori di morte) Flusso dell’informazione genica: la definizione di gene nell’era ENCODE • Replicazione del DNA e mantenimento della sua integrità • Trascrizione del DNA: sintesi degli RNA e loro maturazione • Influenza dell’ambiente sul flusso genico: Epigenetica • Stabilità degli RNA: l’interferenza
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CourseHistology and developmental biology
Course IDMS0732
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersPRAT Maria Giovanna, FOLLENZI Antonia
CFU6
Teaching duration (hours)48
Individual study time 102
SSDBIO/17 - Histology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year1
PeriodSecondo Semestre
Sites and/or partitions
Gruppo A
Gruppo B
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CourseHistology and developmental biology
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPRAT Maria Giovanna
TeachersPRAT Maria Giovanna
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractStudy of the tissues of the body, in their morphological aspects, which are strictly connected with their localizations and functions. Study of the first periods of the development of the human being, of the mechanisms of their origin in the embryo and the interaction of the tissues to form organs. Stem cells from the beginning in the embryo to the adult. Animal models used to study developmental biology. At the end of the course the student must: - have acquired the basic knowledge on the molecular and cellular components, the structure, the function and the embryological origin of the different tissues of the human body - have acquired the knowledge about tissue homeostasis - have acquired basic knowledge on the first steps of the embryonic development (the first two months)
Reference textsWheater: Istologia e Anatomia microscopica, CEA Stevens-Lowe: Istologia Umana, CEA Alberts et al.,: Biologia molecolare della cellula. V ed. Zanichelli Barbieri-Carinci: Embriologia, III ed. CEA De Felici et al.: Embriologia Umana. Piccin
Teaching targetsAt the end of the course the student must have acquired the basic knowledge -on the different tissues of the body, in relation to their localizations and functions. -on the development and morphogenesis of tissues, organs and systems in normal conditions. Moreover the student must know some animal models used to study developmental biology from which general principles for development have been deduced
PrerequisitesThe student must have the basic knowledge of Cell Biology
Didattics MethodsDirect lessons in classroom with the use of files in Presentation format and videos. Students will be able to make copies of some of the files used for the lessons
Grading rulesThe exam consists of a multichoise written proof (about 30-35 questions with only one correct answer). Registration is mandatory. Only correct answers are considered for the final vote.
Full argumentsEpithelial tissue: overview of its structure and functions. Classification. Cell polarity. Cytoplasmic and surface specialization in the different cell membrane domains (apical, lateral, basal): cell junctions and basal membrane. Epithelium histogenesis. Exocrine glands: structure and classification. Specializations of the secretory function. Regulatory mechanisms of secretion. Endocrine glands. Structure, hormones, receptors, their signalling and tissue homeostasis. Pituitary gland and hypothalamo-hypophysial feedback loops. Thyroid gland. Parathyroid glands, Adrenal glands, endocrine pancreas. The blood: plasma, serum. Structure, life and functions of the cells. Leukocyte count, hematocrit. Hemopoiesis: stem cells, progenitor cells and growth factors. Hemocatheresis. Lymphoid tissues: structure and organization of the lymphoid system. Lymphocyte classification; membrane markers. Lymphoid tissues. Lymphopoiesis and lymphocyte maturation. Linfopoiesi e maturazione dei linfociti. Specializzazioni funzionali dei linfociti B, T e plasmacellule. Connective tissue: general structure and functions. The extracellular matrix; the fibers and their synthesis and degradation; the cells; the mesenchymal cells. Classification of connective tissues. Cartilage: structure, classification, chondrogenesis and homeostasis. Bone tissue. General structure and composition of bones. Bone formation, growth, remodeling and repair, Adipose tissue. Muscle tissues: skeletal muscle, cardiac tissue, smooth muscle. Structure and membrane specialization. Mechanism and control of the muscle contraction. Blood vessels: structure. Capillaries and sinusoids. Vasculogenesis and angiogenesis. Nerve tissue. Composition. The neuron: ultrastructural specialization, axonal transport, electric properties of the membrane; synapsis and neurotransmitters; origin of the nerve tissue. Glia and myelin. Gray and white matter. Tissue homeostasis: tissue maintenance and renewal; stem cells and differentiation. Embryology Gametogenesis. Principles of anatomy and histology of the female and male reproductive systems. Germ cells and their maturation. Gametogenesis and their hormonal control. Ovarian and menstrual cycles. Embryo development. Fertilization, zygote, segmentation, blastocyst, twins, blastocyst implantation. Bilaminar and trilaminar embryo. Folding of the embryo. Prospectic development of the three embryonal layers. Fetal annexes. The concept of cell multipotentiality. Determination and differentiation. Position value. - Concetto di potenzialità. Determinazione e differenziazione. Valore di posizione. Development of the body plan: segmentation genes, homeotic genes and identity of the body segments. - Animal modelsl used in the study of developmental biology: Drosophila melanogaster (body plan, homeotic genes); Caernorhabditis elegans (heterochronic genes and genes for apoptosis); chicken and mouse (as reference models to study human development). Principles of organogenesis, examples of the development of an organ (e.g. gonads, kidney). Which can be different depending on the year.
Expected learning objectivesAt the end of the course the student must have acquired the basic knowledge -on the different tissues of the body, in relation to their localizations and functions. -on the development and morphogenesis of tissues, organs and systems in normal conditions. Moreover the student must know some animal models used to study developmental biology from which general principles for development have been deduced.
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CourseHistology and developmental biology
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderFOLLENZI Antonia
TeachersFOLLENZI Antonia
Course typeGruppi
Year1
PartitionGruppo B
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CourseHuman Anatomy
Course IDBT015
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBOCCAFOSCHI Francesca, SABBATINI Maurizio
CFU4
Teaching duration (hours)32
Individual study time 68
SSDBIO/16 - Human anatomy
Course typeModulo di sola Frequenza
Course categoryC - Affine o integrativo
Year1
PeriodSecondo Semestre
Sites and/or partitions
Gruppo A
Gruppo B
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CourseHuman Anatomy
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBOCCAFOSCHI Francesca
TeachersBOCCAFOSCHI Francesca
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractAnatomical terminology, anatomical position, position and movement definition, organs, apparatus and systems. Locomotory apparatus. Generality, structure of bone elements; Type, structure and functional meanings of joints; Structure of striated muscle and smooth muscle, descriptive and functional anatomy of muscle complexes. Cardiovascular system. The heart: position and relationships. Veins and arteries, topographical anatomy of the arterial and venous network and vascularization areas. Respiratory system. Descriptive and functional anatomy of the upper and lower airways (nasal cavities, larynx, trachea and bronchi, pulmonary parenchyma, pleura). Digestive system. Descriptive and functional anatomy of: Buccal Cavity, Faringe, Esophagus, Stomach, Intestine and Glands attached to the Digestive System (Liver, Pancreas, Milza). Urinary tract. Descriptive and functional anatomy of: Kidneys, Renal Pelvis, Uretheres, Bladder, Male and Female Uretra. Genital apparatus. MALE - Descriptive and Functional Anatomy of Testicles, Prostate and Penis. FEMALE - Descriptive and Functional Anatomy of Ovary, Uterus, Vagina. Endocrine system. Descriptive and functional anatomy of: Hypophysis, Thyroid, Parathyroid, Endocrine Pancreas, Surrenal Glands and Gonads. Nervous system. CENTRAL NERVOUS SYSTEM - Descriptive and Functional Anatomy of Spinal Cord, Encephalic Corpus, Cervelletto, Diencefalo, Telencephalo and Core Nuclei. PERIPHERAL NERVOUS SYSTEM - Functional correlations of the sympathetic and parasympathetic system, nomenclature and meaning of the Cranial Nerves. Eye and Ear functional anatomy.
Reference textsAnatomia Umana - Saladin - Casa Editrice Piccin
Teaching targetsThe module provides the students with a theoretical knowledge of the microscopic and macroscopic Human Anatomy with particular reference to the most important anatomical districts for clinical practice and human pathology.
Didattics MethodsPresentations in MS-Power Point format, educational websites. There are interactive exercises for self-assessment of learning during the course.
Grading rulesWritten exam - multiple choice answers
Expected learning objectivesAt the end of the module the student will possess knowledge of the micro and macroscopic organization of the organs, apparatus and systems of the human body, understanding the relationship between structure and function. The students must to communicate their knowledge using correct scientific and anatomical terminology.
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CourseHuman Anatomy
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderSABBATINI Maurizio
TeachersSABBATINI Maurizio
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian language
AbstractThe teaching aims to provide students with a knowledge aboout morphology, topography and function of several organs of human body.
Reference textsANATOMIA UMANA McKinley on-line edition ANATOMIA DELL’UOMO (seconda edizione) Ambrosi et al.; Edi-ermes ANATOMIA DEL GREY (i fondamenti) R.L. Drake et al.; edra ANATOMIA UMANA McKinley; piccin GRANDE ATLANTE DI ANATOMIA UMANA (descrittiva e funzionale, nozioni di istologia e patologia) Viguè-Martin; piccin
Teaching targetsThe aim of the course is to offer to provide students with a knowledge useful to understand the individual, aggregate, and relationship aspects of the human body, which are the basis of human biology and of the pathological manifestations
PrerequisitesStudents must to have the knowledge provided by general biology course
Didattics MethodsTeaching delivered as oral presentation, with Power Point projections auxilium, and discussion in the classroom. The power point projections will be made available to students on the relative DIR pages
Other informations//
Grading rulesThe exam provides a written test that will be submitted to the students to evaluate the acquisition of knowledge in the various subjects of the course. The exam aims to verify the student's ability to focusing on the topics developed by the course, identifying the right response, the student will evidence the acquired knowledgments The written exam consists of 30 multi-choice questions -5 options, of which only one exact- The questions will highlight in 4 topics such as: 6 questions : Bone and Muscles 6 questions : cardiocirculatory apparaus ( 2 heart, 2 arteries , 2 veins or lymphatic system) 12 questions : visceral organs 6 questions : Nervous System. The outcome of the written test identify the vote of the course
Full arguments- Musculoskeletal system. General overview. Appendicular and axil skeleton. structure and functional significance of the joints; the structure of the bone elements; functional and clinical anatomy of the temporomandibular joint. Descriptive and functional anatomy of muscle systems. - Cardiovascular system. The pulmonary and systemic circulation. The heart: anatomy and topography; heart valves veins and arteries, topographical anatomy of arterial and venous vascular territories. Organization of the lymphatic system, lymph and lymph nodes. - Respiratory system. Descriptive and functional anatomy of the upper and lower airways (Nasal Cavity, Larynx, Trachea and Bronchi, Lungs, Pleura). - Digestive system. Descriptive and functional anatomy of the Oral Cavity and Salivary glands, Pharynx, Oesophagus, Stomach, Intestine, Liver, Pancreas, Spleen. - Urinary system. Descriptive and functional anatomy of the Kidneys, Renal pelvis, Ureter, Urinary bladder, male and female urethra. - Genital system. MALE - Descriptive and functional anatomy of the Testis, Prostate and Penis. FEMALE - Descriptive and functional anatomy of the Ovary, Uterus, Vagina. - Endocrine system. Descriptive and functional anatomy of the Hypophysis, Thyroid, Parathyroid, Pancreatic islet, Adrenal glands, Gonads. - Integumentary system descriptive and functional anatomy of the skin, the dermis and skin appendages - Nervous system CENTRAL NERVOUS SYSTEM – Descriptive and functional anatomy of the Spinal cord, Brainstem, Cerebellum, Diencephalon, Telencephalon and Basal Ganglia. PERIPHERAL NERVOUS SYSTEM Cranial and spinal nerves. Functional relationships of the autonomic nervous system (sympathetic and parasympathetic fibers) - Descriptive and functional anatomy of the EYE and EAR
Expected learning objectivesIt is expected that the student will acquire a brief knowledge of topography and complete knowledge of the macro and microarchitectural morphology and function of the various organs of human anatomy. It is also expected that the student has matured the ability to correlate the different reciprocal morpho-functional relationships of the organs.
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CourseInorganic chemistry
Course IDMS0725
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBOCCALERI Enrico, BOTTA Mauro, GABANO Elisabetta, MINASSI Alberto, CAPRIOGLIO Diego
CFU9
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year1
PeriodPrimo Semestre
Grading typeFinal grade
Modules
Course ID Course SSD Teachers
MS0726 General chemistry CHIM/03 - General and inorganic chemistry BOCCALERI Enrico, BOTTA Mauro, GABANO Elisabetta
MS0727 Introductory chemistry laboratory CHIM/06 - Organic chemistry MINASSI Alberto, CAPRIOGLIO Diego
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CourseGeneral chemistry
Course IDMS0726
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBOCCALERI Enrico
TeachersBOCCALERI Enrico, BOTTA Mauro, GABANO Elisabetta
CFU6
Teaching duration (hours)48
Individual study time 102
SSDCHIM/03 - General and inorganic chemistry
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year1
Sites and/or partitions
Gruppo A
Gruppo B
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CourseGeneral chemistry
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBOCCALERI Enrico
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractThe purpose of the course is to provide students the basic knowledge in general and inorganic chemistry. The connection with the various biological disciplines will be discussed, in particular with the use of examples and numerical exercises
Reference textsThe presentations will be available on the D.I.R. site. Suggested Reference Books: -Kotz, Treichel, Townsend, “Chimica” Edises; -Schiavello, Palmisano “Elementi di Chimica” Edises; -Masterton, Hurley: "Chimica: Principi e Reazioni", Piccin; -Tro: "Chimica: un approccio molecolare", Edises; -Whitten, Davis, Peck, Stanley, Chimica (9 Ed.), Piccin. Stoichiometry Reference Books: -Breschi, Massagli: "Stechiometria", Edizioni ETS; -Michelin-Lausarot, Vaglio: "Fondamenti di stechiometria", Piccin; -Uguzzoli: "Come risolvere i problemi di chimica", Casa Editrice Ambrosiana
Teaching targetsThe course aims at introducing the students of the main understanding of chemical phenomena, with details of applicative aspects. A supplementary noteworthy effort will be set on the chemical behavior of aqueous solutions and related chemical reactions, to establish the necessary basis for supplementary chemistry courses and related biological courses.
PrerequisitesNone
Didattics MethodsClassroom lessons in which the main topics reported in the abovementioned program will be disseminated. Course is hereafter completed by practical classroom exercises to tackle a few of the exposed topics.
Other informationsLearning Control: Interactive discussion of the explained topics will be stimulated. Students will be involved in the shared resolution of applicative exercises, to strengthen their knowledge and test actual progresses.
Grading rulesThe final exam is a 2-hour written test, containing questions of both general chemistry (6 questions, each with a maximum score of 2 points) and applied stoichiometry exercises (6 numerical exercises, each one with a maximum score of 3 points). The sum of the points must be at least 18 to pass the examination.
Full argumentsThe matter: physical states, definition of matter (elements, compounds and mixtures). The atomic structure. Atoms and isotopes. Compounds and molecular representations (the laws of definite proportions and of multiple proportions). Atomic and molecular weights. Definition of a.m.u. Avogadro's Number and the concept of mole. Chemical reactions (oxidation numbers, balance of redox and non-redox reactions). The quanto-mechanical description of the atom (orbital concept, quantum numbers, the Aufbau principle). The Periodic Table and its correspondence with the electronic structure of elements. Periodic properties. The chemical bond: the octet rule, ionic bonds, covalent bonds in the theory of valence bond. Hybrid orbitals. The concept of resonance. Bond order and bond lengths, multiple bonds. Electronegativity, polarity of bonds and molecules. Intermolecular forces (ion-dipole, dipole-dipole, hydrogen bonds, induced dipoles, dispersion forces). The aggregation states of matter. Gas: definition and the law of perfect gases. Liquid and solid states. State changes (state diagrams). Solutions: concentrations and colligative properties. Thermodynamics: definitions, the principles of thermodynamics, enthalpy, entropy, free energy. Chemical kinetics: definitions and integrated kinetic laws (zero order, 1st and 2nd order), activation energy and the control of a reaction rate (Arrhenius' law), catalysts. Chemical reactions: the law of the action of masses and Le Chatelier principle, influence of experimental parameters on the chemical constants. Reactions in aqueous solutions. Autoprotonation of water and the pH, acid-base concepts, strong and weak acids and bases (classification and their strength, the structure of most important acids containing N, P, S and halogen elements), hydrolysis, buffer solutions. Products of solubility: definition and applications. Electrochemistry: standard potentials and Nernst's equation, galvanic and electrolitic cells, corrosion. Descriptive Chemistry: metals, semimetals and non-metals. Principal properties of these groups. Stoichiometry exercises related to these issues.
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CourseGeneral chemistry
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBOTTA Mauro, GABANO Elisabetta
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractThe purpose of the course is to provide students the basic knowledge in general and inorganic chemistry. The connection with the various biological disciplines will be discussed, in particular with the use of examples and numerical exercises.
Reference textsThe presentations will be available on the D.I.R. site. Suggested Reference Books: -Kotz, Treichel, Townsend, “Chimica” Edises; -Schiavello, Palmisano “Elementi di Chimica” Edises; -Masterton, Hurley: "Chimica: Principi e Reazioni", Piccin; -Tro: "Chimica: un approccio molecolare", Edises; -Whitten, Davis, Peck, Stanley, Chimica (9 Ed.), Piccin. Stoichiometry Reference Books: -Breschi, Massagli: "Stechiometria", Edizioni ETS; -Michelin-Lausarot, Vaglio: "Fondamenti di stechiometria", Piccin; -Uguzzoli: "Come risolvere i problemi di chimica", Casa Editrice Ambrosiana
Teaching targetsThe course aims at introducing the students of the main understanding of chemical phenomena, with details of applicative aspects. A supplementary noteworthy effort will be set on the chemical behavior of aqueous solutions and related chemical reactions, to establish the necessary basis for supplementary chemistry courses and related biological courses.
PrerequisitesBasic (high school) knowledge of mathematics and physics
Didattics MethodsClassroom lessons in which the main topics reported in the abovementioned program will be disseminated. Course is hereafter completed by practical classroom exercises to tackle a few of the exposed topics.
Other informationsLearning Control: Interactive discussion of the explained topics will be stimulated. Students will be involved in the shared resolution of applicative exercises, to strengthen their knowledge and test actual progresses.
Grading rulesThe exam of the whole course consists of a 2-hour written exam that evaluates the student's ability to apply the knowledge acquired during the course. This test will consist of 6 numerical (stoichiometric) exercises and 6 questions (theory). For the stoichiometry part, the exercises may include: the balance of chemical reactions, gas laws, property of the solutions, acid-base properties, solubility, electrochemistry. For the theoretical part, the questions may include: the electronic structure of the atoms, the periodic system, the chemical bond, the intermolecular forces and the physical states of matter, thermodynamics and kinetics, acids and bases. The exercises will be chosen so that the entire program is covered, and the student can demonstrate that they know and understand at least the basic concepts through their applications. The difficulty level of the exercises corresponds to the program and the reference texts indicated. Each of the six stechiometric exercises will be awarded max 3 points, while each of the six theoretical exercises will be awarded max 2 points (if error-free). The written test will be passed if the sum of the scores is not less than 18 points (18/30).
Full argumentsThe matter: physical states, definition of matter (elements, compounds and mixtures). The atomic structure. Atoms and isotopes. Compounds and molecular representations (the laws of definite proportions and of multiple proportions). Atomic and molecular weights. Definition of a.m.u. Avogadro's Number and the concept of mole. Chemical reactions (oxidation numbers, balance of redox and non-redox reactions). The quanto-mechanical description of the atom (orbital concept, quantum numbers, the Aufbau principle). The Periodic Table and its correspondence with the electronic structure of elements. Periodic properties. The chemical bond: the octet rule, ionic bonds, covalent bonds in the theory of valence bond. Hybrid orbitals. The concept of resonance. Bond order and bond lengths, multiple bonds. Electronegativity, polarity of bonds and molecules. Intermolecular forces (ion-dipole, dipole-dipole, hydrogen bonds, induced dipoles, dispersion forces). The aggregation states of matter. Gas: definition and the law of perfect gases. Liquid and solid states. State changes (state diagrams). Solutions: concentrations and colligative properties. Thermodynamics: definitions, the principles of thermodynamics, enthalpy, entropy, free energy. Chemical kinetics: definitions and integrated kinetic laws (zero order, 1st and 2nd order), activation energy and the control of a reaction rate (Arrhenius' law), catalysts. Chemical reactions: the law of the action of masses and Le Chatelier principle, influence of experimental parameters on the chemical constants. Reactions in aqueous solutions, autoprotonation of water and the pH, acid-base concepts, strong and weak acids and bases (classification and their strength, the structure of most important acids containing N, P, S and halogen elements), hydrolysis, buffer solutions [this part will be explained in a specific CFU]. Products of solubility: definition and applications. Electrochemistry: standard potentials and Nernst's equation, galvanic and electrolitic cells, corrosion. Descriptive Chemistry: metals, semimetals and non-metals. Principal properties of these groups. Stoichiometry exercises related to these issues.
Expected learning objectivesKnowledge and understanding: theoretical and operational knowledge about the fundamental laws of chemistry (mol, reaction, bonds and molecular structure, states of matter, solutions, equilibrium, solution pH, thermodynamics, kinetics, electrochemistry); acquisition of a vocabulary of chemical terms to be able to expose chemical-scientific topics in a precise, concise and clear manner. Applying knowledge and understanding: ability to apply the theory to assign of the names of the most common inorganic chemical compounds, to balance a chemical reaction, to perform stoichiometric calculations, to solve numerical problems with gases, solutions, and acid or basic substances, and electrochemistry; ability to correlate the chemical structure with the physical properties and reactivity of the compounds. Making judgements: ability to interpret and rationalize chemical reactions from a critical and non-mnemonic point of view, using a scientific methodological approach to be applied to subsequent studies in other fields of chemistry and biology. Communication skills: ability to use a suitable scientific language during the learning control in the classroom.
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CourseIntroductory chemistry laboratory
Course IDMS0727
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMINASSI Alberto
TeachersMINASSI Alberto, CAPRIOGLIO Diego
CFU3
Teaching duration (hours)16
Individual study time 47
SSDCHIM/06 - Organic chemistry
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year1
Sites and/or partitions
Gruppo A
Gruppo B
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CourseIntroductory chemistry laboratory
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersMINASSI Alberto
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageitalian
AbstractMolarity, Normality, Molality,% p / p, p / v, v / v, pH and factors affecting pH, acidity and constant acid dissocation, buffer solution, quantitative analysis, volumetric analysis (titrations)
Reference textsno specific text.
Teaching targetsThe objective of the theoretical lessons is the knowledge and the method of using the basic glassware used in chemical laboratories, stoichiometric calculations and well-known preparation preparations, volumetric analysis (titration) principles and laboratory safety. Laboratory practice: putting into practice the knowledge gained during the theoretical lessons.
Prerequisitesnone
Didattics MethodsClassroom lessons. Copy of the transparencies used during the course lessons available on the site. Exercises in the classroom. laboratory exercises
Grading ruleswritten exam where the ability to calculate the properties of solutions.
Full argumentsCFU 1 • Molarity • Normality • Molality •% p / p, p / v, v / v • pH and factors affecting pH • Buffer solution • Quantitative analysis • Volumetric analysis (titrations) CFU 2 Basic equipment in a chemical laboratory, glassware and its use, plastics and its use, scales, stoichiometric calculations, prediction of ionization, concentration, pH and related calculations. Safety and behavioral rules in the laboratory. CFU 3 Practical exercises in the lab
Expected learning objectivesthe student at the end of the course must have learned the main analytical laboratory techniques. In addition to this he must have learned how to calculate the main properties of the solutions.
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CourseIntroductory chemistry laboratory
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersCAPRIOGLIO Diego
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageitalian
AbstractMolarity, Normality, Molality,% p / p, p / v, v / v, pH and factors affecting pH, acidity and constant acid dissocation, buffer solution, quantitative analysis, volumetric analysis (titrations)
Reference textsno specific text.
Teaching targetsThe objective of the theoretical lessons is the knowledge and the method of using the basic glassware used in chemical laboratories, stoichiometric calculations and well-known preparation preparations, volumetric analysis (titration) principles and laboratory safety. Laboratory practice: putting into practice the knowledge gained during the theoretical lessons.
Prerequisitesnone
Didattics MethodsClassroom lessons. Copy of the transparencies used during the course lessons available on the site. Exercises in the classroom. laboratory exercises
Grading ruleswritten exam where the ability to calculate the properties of solutions.
Full argumentsCFU 1 • Molarity • Normality • Molality •% p / p, p / v, v / v • pH and factors affecting pH • Buffer solution • Quantitative analysis • Volumetric analysis (titrations) CFU 2 Basic equipment in a chemical laboratory, glassware and its use, plastics and its use, scales, stoichiometric calculations, prediction of ionization, concentration, pH and related calculations. Safety and behavioral rules in the laboratory. CFU 3 Practical exercises in the lab
Expected learning objectivesthe student at the end of the course must have learned the main analytical laboratory techniques. In addition to this he must have learned how to calculate the main properties of the solutions.
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CourseOrganic Chemistry
Course IDMS0733
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersTEI Lorenzo, NEGRI Roberto, APPENDINO Giovanni Battista
CFU6
Teaching duration (hours)48
Individual study time 102
SSDCHIM/06 - Organic chemistry
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryA - Base
Year1
PeriodSecondo Semestre
SiteNOVARA
Grading typeFinal grade
Sites and/or partitions
Gruppo B
Gruppo A
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CourseOrganic Chemistry
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderTEI Lorenzo
TeachersTEI Lorenzo, NEGRI Roberto
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractThe course aims at providing students the basic knowledge of organic chemistry necessary to the study of the biological mechanisms involved in cell metabolism and its regulation. It involves a structural section (nomenclature, functional groups, stereochemistry, acid-base and redox properties) and a mechanistic section where the main mechanisms involved in reactivity are systematically addressed, exemplifying them with the description of the main classes of organic compounds.
Reference textsBotta Chimica Organica (second edition) Edi-Ermes W.H. Brown – T Poon, Introduzione alla Chimica Organica; EdiSES McMurry – Fondamenti di chimica organica – Zanichelli. For the exercises: D’Auria, Taglialatela-Scafati, Zampella: Guida ragionata allo svolgimento di esercizi di chimica organica (seconda edizione), Loghia. The slides used in the course are available on the DIR platform.
Teaching targetsa) To recognize functional groups and predict topological and reactivity properties of organic compounds. b) To master polar reactivity mechanisms: (aliphatic and aromatic nucleophilic substitution, acyl nucleophilic substitution, aromatic electrophilic substitution, electrophilic addition, nucleophilic addition, elimination) and the basic concepts of radical reactivity.
PrerequisitesBasic knowledge of general chemistry
Didattics MethodsThe lessons will use both slide projection (available in Powerpoint format on line via DIR) and writing on the blackboard.
Other informationsExercises sections preparatory to the exam will be held on regular basis to complement the mainstream lessons. Particular emphasis will be given to multiple-choice questions.
Grading rulesThe exam is based on a 20-multiple-choice question test carried out with a computer.
Full argumentsCredit # 1: Structure and properties 1. Chemical bond 2. Ibridization and polarity of bonds 3. Covalent, ionic and covalent polar bonds 4. Lewis structural formulas, formal charges and resonance. 5. Aromaticity 6. Exercises -1 7. Exercises-2 8. Exercises-3 Credit # 2: Functional groups, properties and nomenclature 9. Functional groups and their physical and chemical (acidity and basicity) properties. 10. Nomenclature 11. Inter- and intramolecular interactions 12. Exercises-1 13. Exercises-2 14. Exercises -3 15. Exercises-4 16. Exercises-5- Credit #3: Isomerism, conformational analysis and stereochemitry 17. Isomerism. Definition and classification 18. Conformational analysis of alkanes. Configurational analysis of alkenes. 19. Chirality and configurational analysis:-1 20. Chirality and configurational analysis:-2 21. Exercises-1 22. Exercises-2 23. Exercises-3 24. Exercises-4 Credit # 4: Functional groups and their reactivity-1 25. Reactivity of alkanes, alkenes and alkyes: electrophilic addition and catalytic hydrogenation and radical substitution. 26. Alkyl halides and nucleophilic substitution reaction (SN1 and SN2). 27. Elimination reaction and competion with SN, 28. Alcohols and their reactivity 29. Exercises-1 30. Exercises-2 31. Exercises-3 32. Exercises-4 Credit #5: Functional groups and their reactivity-2 33. Ethers, epoxides, thiols and thioethers. 34. Amines 35. Carbonyl derivatives and nucleophilic addition 36. Tautomerism and condensation reactions. 37. Carboxylic acids and acyl nucleophilic substitution 38. Caarboxylic acid derivatives 39. Exercises-1 40. Exercises-2 Credito #6: Functional groups and their reactivity-3 & Biomolecoler 41. Benzene: and aromtic electrophic substitution 42. Phenols and aromatic amines. Nucleophilic aromatic substitutio 43. Exercises-1. 44. Exercises-2 45. Amino acids and peptides-1 46. Amino acids and peptidesi-2 47. Sugas 48. Lipids
Expected learning objectivesAbility to “read” behind the formula of an organic compounds, making previsions on its properties and reactivity.
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CourseOrganic Chemistry
Academic Year2017/2018
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderAPPENDINO Giovanni Battista
TeachersAPPENDINO Giovanni Battista
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractThe course aims at providing students the basic knowledge of organic chemistry necessary to the study of the biological mechanisms involved in cell metabolism and its regulation. It involves a structural section (nomenclature, functional groups, stereochemistry, acid-base and redox properties) and a mechanistic section where the main mechanisms involved in reactivity are systematically addressed, exemplifying them with the description of the main classes of organic compounds.
Reference textsBotta Chimica Organica (second edition) Edi-Ermes W.H. Brown – T Poon, Introduzione alla Chimica Organica; EdiSES McMurry – Fondamenti di chimica organica – Zanichelli. For the exercises: D’Auria, Taglialatela-Scafati, Zampella: Guida ragionata allo svolgimento di esercizi di chimica organica (seconda edizione), Loghia The slides used in the course are available on the DIR platform.
Teaching targetsa) To recognize functional groups and predict topological and reactivity properties of organic compounds. b) To master polar reactivity mechanisms: (aliphatic and aromatic nucleophilic substitution, acyl nucleophilic substitution, aromatic electrophilic substitution, electrophilic addition, nucleophilic addition, elimination) and the basic concepts of radical reactivity.
PrerequisitesBasic knowledge of general chemistry
Didattics MethodsThe lessons will use both slide projection (available in Powerpoint format on line via DIR) and writing on the blackboard.
Other informationsExercises sections preparatory to the exam will be held on regular basis to complement the mainstream lessons. Particular emphasis will be given to multiple-choice questions.
Grading rulesThe exam is based on a 20-multiple-choice question test carried out with a computer.
Full argumentsCredit # 1: Structure and properties 1. Chemical bond 2. Ibridization and polarity of bonds 3. Covalent, ionic and covalent polar bonds 4. Lewis structural formulas, formal charges and resonance. 5. Aromaticity 6. Exercises -1 7. Exercises-2 8. Exercises-3 Credit # 2: Functional groups, properties and nomenclature 9. Functional groups and their physical and chemical (acidity and basicity) properties. 10. Nomenclature 11. Inter- and intramolecular interactions 12. Exercises-1 13. Exercises-2 14. Exercises -3 15. Exercises-4 16. Exercises-5- Credit #3: Isomerism, conformational analysis and stereochemitry 17. Isomerism. Definition and classification 18. Conformational analysis of alkanes. Configurational analysis of alkenes. 19. Chirality and configurational analysis:-1 20. Chirality and configurational analysis:-2 21. Exercises-1 22. Exercises-2 23. Exercises-3 24. Exercises-4 Credit # 4: Functional groups and their reactivity-1 25. Reactivity of alkanes, alkenes and alkyes: electrophilic addition and catalytic hydrogenation and radical substitution. 26. Alkyl halides and nucleophilic substitution reaction (SN1 and SN2). 27. Elimination reaction and competion with SN, 28. Alcohols and their reactivity 29. Exercises-1 30. Exercises-2 31. Exercises-3 32. Exercises-4 Credit #5: Functional groups and their reactivity-2 33. Ethers, epoxides, thiols and thioethers. 34. Amines 35. Carbonyl derivatives and nucleophilic addition 36. Tautomerism and condensation reactions. 37. Carboxylic acids and acyl nucleophilic substitution 38. Caarboxylic acid derivatives 39. Exercises-1 40. Exercises-2 Credito #6: Functional groups and their reactivity-3 & Biomolecoler 41. Benzene: and aromtic electrophic substitution 42. Phenols and aromatic amines. Nucleophilic aromatic substitutio 43. Exercises. 44. Radical oxidation and active oxygen species 45. Amino acids and peptides-1 46. Amino acids and peptidesi-2 47. Sugas 48. Lipids
Expected learning objectivesCapacity to “read” behind the formula of an organic compounds, making previsions on its properties and reactivity
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CourseFunctional biochemistry with elements of laboratory
Course IDBT068
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBALDANZI Gianluca
TeachersCAPELLO Daniela, BALDANZI Gianluca, BERTONI Alessandra
CFU11
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year2
PeriodPrimo Semestre
SiteNOVARA
Grading typeFinal grade
Teaching languageItalian
AbstractFunctional Biochemistry Introduction (Capello) • Metabolism and bioenergy • Vitamins and coenzymes • Insulin and glucagon Functional Biochemistry 1. Carbohydrate digestion with aerobic and anaerobic glycolysis 2. Piruvate dehydrogenase and tricharboxylic acid cycle 3. Oxidative phosphorylation and ROS 4. Pentosophosphates + gluconeogenesis 5. Glycogen metabolism 6. Adjust glucose and glucose catabolism 7. Glycogenicity and glycemia 8. Lipids: digestion, transposed and lipoproteins, fatty acid catabolism 9. Catabolism odd C fatty acids, peroxisome ketone bodies and unsaturated fatty acids 10. Fatty acid and phospholipid biosynthesis with sphingolipid hints 11. Cholesterol: synthesis and catabolism to biliary acids 12. Steroid hormones, production and physiological roles 13. Photosynthesis and Calvin cycle 14. Organizing nitrogen and amino acid biosynthesis, essential amino acids 15. Protein digestion, blood transport (alanine, glutamine), amino acid degradation by example (tyrosine), urea cycle 16. Amino acid derivatives: bioactive amines, NO, eme 17. Anabolic Nucleotides and Dosoxinucleotides with Adjustment 18. Catabolism nucleotides and uric acid 19. Integration nutrition / fasting cycle metabolism Laboratory Measuring biological things. Techniques for sample preparation in biochemistry Spectrophotometry. Chromatography Protein characterization. Using antibodies in biotechnology. Enzymes in diagnostic and research. Binding assays.
Reference textsPrincipi di biochimica di Lehninger di Lehninger - Nelson - Cox • 2014; VI edizione, Zanichelli Biochimica medica strutturale metabolica e funzionale di Noris Siliprandi, Guido Tettamanti. IV edizione, PICCIN PRINCIPI DI METODOLOGIA BIOCHIMICA di C. De Marco, C. Cini – Ed. Piccin METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI di REED Rob , HOLMES David , WEYERS Jonathan , JONES Allan - 2002 Zanichelli Editore METODOLOGIE DI BASE PER LA BIOCHIMICA E LA BIOTECNOLOGIA di NINFA Alexander J , BALLOU David P - 2000 Zanichelli Editore
Teaching targetsUnderstand and explain at the molecular, subcellular, cellular and tissue levels the biochemical mechanisms involved in digestion, absorption, transport, storage, catabolism, interconversion, excretion, biosynthesis of carbohydrates, amino acids and proteins, lipids, nucleotides, even in relation to different functional states of the body - Transfer the student to a solid knowledge of the hormonal regulation mechanisms of the major biochemical processes associated with the various biological functions. - Learning basic methodologies in the biochemistry laboratory.
PrerequisitesGeneral and organic chemistry. Basis of physics, chemistry laboratory and biochemistry.
Didattics MethodsIn class theory lessons and laboratory exercitations. Slide show during lessons. For the preparation of the exam, students will be able to use the material provided by the teacher (pdf copy of the slides delivered to the lesson and any deliveries that deepen the topics discussed during the course) and the recommended textbooks
Other informationsSlides and information are available on DIR (https://www.dir.uniupo.it/ )
Grading rulesThe exam is divided into a written part with multiple choice answers (crosswords) and / or true / false answers) and an optional oral part. The frequency of laboratory exercitations is mandatory. By the end of the exercitations, the student will present a laboratory book that will be evaluated together with student’s productivity in the laboratory.
Full argumentsBioenergetics and types of biochemical reactions: Bioenergetics and thermodynamics. Chemical logic and most common biochemical reactions. Transfers of phosphorous and ATP groups. The biological reactions of oxidation reduction Glycolysis, gluconeogenesis and pathway of phosphate pentose: Glycolysis. Glycolysis supply routes. The fate of pyruvate under anaerobic conditions: fermentation. The gluconeogenesis. The oxidation of glucose through the pathway of pentose phosphate. Coordinated regulation of glycolysis and gluconeogenesis. Glycogen metabolism in animals, Coordinated regulation of glycogen synthesis and demolition The citric acid cycle: Production of acetyl-CoA (activated acetate). Citric acid cycle reactions. Adjustment of the citric acid cycle. The glyoxylate cycle Oxidative phosphorylation: Electron flow in mitochondria, ATP synthesis, Oxidative phosphorylation regulation, Mitochondria in thermogenesis, synthesis of steroids and apoptosis. Catabolism of fatty acids: Digestion, mobilization and transport of fatty acids. Oxygenation of fatty acids. The ketone bodies. Lipid Biosynthesis: Biosynthesis of fatty acids. Biosynthesis of triacylglycerols. 3 Membrane phospholipid biosynthesis. Cholesterol, steroids and isoprenoids: biosynthesis, regulation and transport Oxidation of amino acids and production of urea: Metabolic Destiny of Amine Groups. Nitrogen excretion and urea cycle. Degradation routes of amino acids. Biosynthesis of amino acids, nucleotides and related molecules PHOTOSYSTEMS: LIGHTING ENERGY CATTURE: Light absorption and photophosphorylation, The central photochemical event: light-induced electron flow. Synthesis of ATP coupled to photophosphorylation. Carbohydrate biosynthesis in plants and bacteria Metabolic control principles: Metabolic pathway regulation. Analysis of metabolic control. Hormone regulation and metabolism integration into mammals Techniques for sample preparation in biochemistry Spectrophotometry. Chromatography Protein characterization. Using antibodies in biotechnology. Enzymes in diagnostic and research. Binding assays.
Expected learning objectivesAn in-depth knowledge of metabolism, biotechnological implications, and metabolic regulation. The student will acquire the knowledge of the main biochemical methods with particular emphasis on the study of proteins and critical reading of literature. Exercises allow you to move efficiently in the lab even in view of the final thesis preparation.
Modules
Course ID Course SSD Teachers
BT038 Functional biochemistry BIO/10 - Biochemistry BALDANZI Gianluca, CAPELLO Daniela, BERTONI Alessandra
MS0444 Laboratory of Biochemical Methods and proteomic BIO/10 - Biochemistry BALDANZI Gianluca, BERTONI Alessandra
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CourseFunctional biochemistry
Course IDBT038
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBALDANZI Gianluca
TeachersCAPELLO Daniela, BALDANZI Gianluca, BERTONI Alessandra
CFU6
Teaching duration (hours)48
Individual study time 102
SSDBIO/10 - Biochemistry
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CourseFunctional biochemistry
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBALDANZI Gianluca
TeachersCAPELLO Daniela, BALDANZI Gianluca
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
Abstractntroduction (Capello) • Metabolism and bioenergy • Vitamins and coenzymes • Insulin and glucagon Functional Biochemistry 1. Carbohydrate digestion with aerobic and anaerobic glycolysis 2. Piruvate dehydrogenase and tricharboxylic acid cycle 3. Oxidative phosphorylation and ROS 4. Pentosophosphates + gluconeogenesis 5. Glycogen metabolism 6. Adjust glucose and glucose catabolism 7. Glycogenicity and glycemia 8. Lipids: digestion, transposed and lipoproteins, fatty acid catabolism 9. Catabolism odd C fatty acids, peroxisome ketone bodies and unsaturated fatty acids 10. Fatty acid and phospholipid biosynthesis with sphingolipid hints 11. Cholesterol: synthesis and catabolism to biliary acids 12. Steroid hormones, production and physiological roles 13. Photosynthesis and Calvin cycle 14. Organizing nitrogen and amino acid biosynthesis, essential amino acids 15. Protein digestion, blood transport (alanine, glutamine), amino acid degradation by example (tyrosine), urea cycle 16. Amino acid derivatives: bioactive amines, NO, eme 17. Anabolic Nucleotides and Dosoxinucleotides with Adjustment 18. Catabolism nucleotides and uric acid 19. Integration nutrition / fasting cycle metabolism Google Traduttore per le aziende:Translator ToolkitTraduttore di siti web
Reference textsPrincipi di biochimica di Lehninger di Lehninger - Nelson - Cox • 2014; VI edizione, Zanichelli Biochimica medica strutturale metabolica e funzionale di Noris Siliprandi, Guido Tettamanti. IV edizione, PICCIN
Teaching targetsUnderstand and explain at the molecular, subcellular, cellular and tissue levels the biochemical mechanisms involved in digestion, absorption, transport, storage, catabolism, interconversion, excretion, biosynthesis of carbohydrates, amino acids and proteins, lipids, nucleotides, even in relation to different functional states of the body - Transfer the student to a solid knowledge of the hormonal regulation mechanisms of the major biochemical processes associated with the various biological functions.
PrerequisitesGeneral and organic chemistry
Didattics MethodsSlide show during lessons. For the preparation of the exam, students will be able to use the material provided by the teacher (pdf copy of the slides delivered to the lesson and any deliveries that deepen the topics discussed during the course) and the recommended textbooks
Other informationsCourse program and slides are available on DIR (https://www.dir.uniupo.it/ )
Grading rulesThe exam is divided into a written part with multiple choice answers (crosswords) and / or true / false answers) and an optional oral part.
Full argumentsBioenergetics and types of biochemical reactions: Bioenergetics and thermodynamics. Chemical logic and most common biochemical reactions. Transfers of phosphorous and ATP groups. The biological reactions of oxidation reduction Glycolysis, gluconeogenesis and pathway of phosphate pentose: Glycolysis. Glycolysis supply routes. The fate of pyruvate under anaerobic conditions: fermentation. The gluconeogenesis. The oxidation of glucose through the pathway of pentose phosphate. Coordinated regulation of glycolysis and gluconeogenesis. Glycogen metabolism in animals, Coordinated regulation of glycogen synthesis and demolition The citric acid cycle: Production of acetyl-CoA (activated acetate). Citric acid cycle reactions. Adjustment of the citric acid cycle. The glyoxylate cycle Oxidative phosphorylation: Electron flow in mitochondria, ATP synthesis, Oxidative phosphorylation regulation, Mitochondria in thermogenesis, synthesis of steroids and apoptosis. Catabolism of fatty acids: Digestion, mobilization and transport of fatty acids. Oxygenation of fatty acids. The ketone bodies. Lipid Biosynthesis: Biosynthesis of fatty acids. Biosynthesis of triacylglycerols. 3 Membrane phospholipid biosynthesis. Cholesterol, steroids and isoprenoids: biosynthesis, regulation and transport Oxidation of amino acids and production of urea: Metabolic Destiny of Amine Groups. Nitrogen excretion and urea cycle. Degradation routes of amino acids. Biosynthesis of amino acids, nucleotides and related molecules PHOTOSYSTEMS: LIGHTING ENERGY CATTURE: Light absorption and photophosphorylation, The central photochemical event: light-induced electron flow. Synthesis of ATP coupled to photophosphorylation. Carbohydrate biosynthesis in plants and bacteria Metabolic control principles: Metabolic pathway regulation. Analysis of metabolic control. Hormone regulation and metabolism integration into mammals
Expected learning objectivesAn in-depth knowledge of metabolism, biotechnological implications, and metabolic regulation.
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CourseFunctional biochemistry
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBERTONI Alessandra
TeachersCAPELLO Daniela, BERTONI Alessandra
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
AbstractIntroduction (Capello) • Metabolism and bioenergy • Vitamins and coenzymes • Insulin and glucagon Functional Biochemistry 1. Carbohydrate digestion with aerobic and anaerobic glycolysis 2. Piruvate dehydrogenase and tricharboxylic acid cycle 3. Oxidative phosphorylation and ROS 4. Pentosophosphates + gluconeogenesis 5. Glycogen metabolism 6. Adjust glucose and glucose catabolism 7. Glycogenicity and glycemia 8. Lipids: digestion, transposed and lipoproteins, fatty acid catabolism 9. Catabolism odd C fatty acids, peroxisome ketone bodies and unsaturated fatty acids 10. Fatty acid and phospholipid biosynthesis with sphingolipid hints 11. Cholesterol: synthesis and catabolism to biliary acids 12. Steroid hormones, production and physiological roles 13. Photosynthesis and Calvin cycle 14. Organizing nitrogen and amino acid biosynthesis, essential amino acids 15. Protein digestion, blood transport (alanine, glutamine), amino acid degradation by example (tyrosine), urea cycle 16. Amino acid derivatives: bioactive amines, NO, eme 17. Anabolic Nucleotides and Dosoxinucleotides with Adjustment 18. Catabolism nucleotides and uric acid 19. Integration nutrition / fasting cycle metabolism
Reference textsPrincipi di biochimica di Lehninger di Lehninger - Nelson - Cox • 2014; VI edizione, Zanichelli Biochimica medica strutturale metabolica e funzionale di Noris Siliprandi, Guido Tettamanti. IV edizione, PICCIN Understand and explain at the molecular, subcellular, cellular and tissue levels the biochemical mechanisms involved in digestion, absorption, transport, storage, catabolism, interconversion, excretion, biosynthesis of carbohydrates, amino acids and proteins, lipids, nucleotides, even in relation to different functional states of the body - Transfer the student to a solid knowledge of the hormonal regulation mechanisms of the major biochemical processes associated with the various biological functions.
Teaching targets- Conoscere e spiegare a livello molecolare, subcellulare, cellulare e tissutale i meccanismi biochimici coinvolti nei processi di: digestione, assorbimento, trasporto, deposito, catabolismo, interconversioni, escrezione, biosintesi di: carboidrati, amminoacidi e proteine, lipidi, nucleotidi, gruppo eme, anche in relazione a diversi stati funzionali dell’organismo - Trasferire allo studente solide conoscenze dei meccanismi di regolazione ormonale dei principali processi biochimici associati alle diverse funzioni biologiche.
PrerequisitesGeneral and organic chemistry
Didattics MethodsSlide show during lessons. For the preparation of the exam, students will be able to use the material provided by the teacher (pdf copy of the slides delivered to the lesson and any deliveries that deepen the topics discussed during the course) and the recommended textbooks
Other informationsSlides and information are available on DIR (https://www.dir.uniupo.it/ )
Grading rulesThe exam is divided into a written part with multiple choice answers (crosswords) and / or true / false answers) and an optional oral part.
Full argumentsBioenergetics and types of biochemical reactions: Bioenergetics and thermodynamics. Chemical logic and most common biochemical reactions. Transfers of phosphorous and ATP groups. The biological reactions of oxidation reduction Glycolysis, gluconeogenesis and pathway of phosphate pentose: Glycolysis. Glycolysis supply routes. The fate of pyruvate under anaerobic conditions: fermentation. The gluconeogenesis. The oxidation of glucose through the pathway of pentose phosphate. Coordinated regulation of glycolysis and gluconeogenesis. Glycogen metabolism in animals, Coordinated regulation of glycogen synthesis and demolition The citric acid cycle: Production of acetyl-CoA (activated acetate). Citric acid cycle reactions. Adjustment of the citric acid cycle. The glyoxylate cycle Oxidative phosphorylation: Electron flow in mitochondria, ATP synthesis, Oxidative phosphorylation regulation, Mitochondria in thermogenesis, synthesis of steroids and apoptosis. Catabolism of fatty acids: Digestion, mobilization and transport of fatty acids. Oxygenation of fatty acids. The ketone bodies. Lipid Biosynthesis: Biosynthesis of fatty acids. Biosynthesis of triacylglycerols. 3 Membrane phospholipid biosynthesis. Cholesterol, steroids and isoprenoids: biosynthesis, regulation and transport Oxidation of amino acids and production of urea: Metabolic Destiny of Amine Groups. Nitrogen excretion and urea cycle. Degradation routes of amino acids. Biosynthesis of amino acids, nucleotides and related molecules PHOTOSYSTEMS: LIGHTING ENERGY CATTURE: Light absorption and photophosphorylation, The central photochemical event: light-induced electron flow. Synthesis of ATP coupled to photophosphorylation. Carbohydrate biosynthesis in plants and bacteria Metabolic control principles: Metabolic pathway regulation. Analysis of metabolic control. Hormone regulation and metabolism integration into mammals
Expected learning objectivesAn in-depth knowledge of metabolism, biotechnological implications, and metabolic regulation.
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CourseLaboratory of Biochemical Methods and proteomic
Course IDMS0444
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBALDANZI Gianluca, BERTONI Alessandra
CFU5
Teaching duration (hours)25
Individual study time 80
SSDBIO/10 - Biochemistry
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year2
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CourseLaboratory of Biochemical Methods and proteomic
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBALDANZI Gianluca
TeachersBALDANZI Gianluca
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
AbstractMeasuring biological things. Techniques for sample preparation in biochemistry Spectrophotometry. Chromatography Protein characterization. Using antibodies in biotechnology. Enzymes in diagnostic and research. Binding assays.
Reference textsPRINCIPI DI METODOLOGIA BIOCHIMICA di C. De Marco, C. Cini – Ed. Piccin METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI di REED Rob , HOLMES David , WEYERS Jonathan , JONES Allan - 2002 Zanichelli Editore METODOLOGIE DI BASE PER LA BIOCHIMICA E LA BIOTECNOLOGIA di NINFA Alexander J , BALLOU David P - 2000 Zanichelli Editore
Teaching targetsLearning basic methodologies in the biochemistry laboratory.
PrerequisitesBasis of physics, chemistry, chemistry laboratory and biochemistry.
Didattics MethodsIn class theory lessons and laboratory exercitations.
Grading rulesThe frequency of laboratory exercitations is mandatory. By the end of the exercitations, the student will present a laboratory book that will be evaluated together with student’s productivity in the laboratory. The integrated course has a single written + oral exam.
Full argumentsMeasuring biological things. Techniques for sample preparation in biochemistry Spectrophotometry. Chromatography Protein characterization. Using antibodies in biotechnology. Enzymes in diagnostic and research. Binding assays.
Expected learning objectivesThe student will acquire the knowledge of the main biochemical methods with particular emphasis on the study of proteins and critical reading of literature. Exercises allow you to move efficiently in the lab even in view of the final thesis preparation.
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CourseLaboratory of Biochemical Methods and proteomic
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBERTONI Alessandra
TeachersBERTONI Alessandra
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
AbstractMeasuring biological things. Techniques for sample preparation in biochemistry Spectrophotometry. Chromatography Protein characterization. Using antibodies in biotechnology. Enzymes in diagnostic and research. Binding assays.
Reference textsPRINCIPI DI METODOLOGIA BIOCHIMICA di C. De Marco, C. Cini – Ed. Piccin METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI di REED Rob , HOLMES David , WEYERS Jonathan , JONES Allan - 2002 Zanichelli Editore METODOLOGIE DI BASE PER LA BIOCHIMICA E LA BIOTECNOLOGIA di NINFA Alexander J , BALLOU David P - 2000 Zanichelli Editore
Teaching targetsLearning basic methodologies in the biochemistry laboratory.
PrerequisitesBasis of physics, chemistry, chemistry laboratory and biochemistry.
Didattics MethodsIn class theory lessons and laboratory exercitations.
Grading rulesThe frequency of laboratory exercitations is mandatory. By the end of the exercitations, the student will present a laboratory book that will be evaluated together with student’s productivity in the laboratory. The integrated course has a single written + oral exam.
Full argumentsMeasuring biological things. Techniques for sample preparation in biochemistry Spectrophotometry. Chromatography Protein characterization. Using antibodies in biotechnology. Enzymes in diagnostic and research. Binding assays.
Expected learning objectivesThe student will acquire the knowledge of the main biochemical methods with particular emphasis on the study of proteins and critical reading of literature. Exercises allow you to move efficiently in the lab even in view of the final thesis preparation.
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CourseFundamentals of immunology and medical microbiology
Course IDBT027
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderAZZIMONTI Barbara
TeachersDIANZANI Umberto, CHIOCCHETTI Annalisa, AZZIMONTI Barbara
CFU10
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year2
PeriodSecondo Semestre
Grading typeFinal grade
Teaching languageThe course is held in Italian. The power point slides can be in both Italian and English. The projected visual experiments are in English
AbstractConsistent with the specific training objectives of the CdS, reported in the SUA-CdS, framework A4.a, the contents of Medical Microbiology are described below: The bacterial cell: organization and structure. Division and growth. Bacterial metabolism. Resident microbial flora: the microbiota. Principles of bacterial virulence: Bacterial toxins: exotoxins and endotoxins; the spore; the capsule; the pili and the scourges; enzymes; the siderophores; the bacterial biofilm. Mechanisms of gene transfer: conjugation, transformation, transduction, transposition. Basics of the main pathogenic bacteria: Staphylococci, Streptococci, Enterobacteriaceae, Clostridia, Mycobacteria, Neisseria, Pseudomonas, A. Baumannii, Pneumococci, Chlamydia and Helicobacteria. Viruses: organization and structure, cultivation and multiplication, pathogenicity. Basics of the main pathogenic viruses (these topics will be explored during the Master's Degree in the Course of Molecular Virology): Hepadnavirus and other viruses causing hepatitis, Herpesvirus, Papillomavirus, Orthomyxovirus, Retrovirus. Basics of subviral agents: prions. Basics on eukaryotic microorganisms: the protists. Control of microbial growth: Disinfection and sterilization; antibiotics; natural antimicrobials; antiviral drugs; resistance mechanisms (superbugs). Vaccines: anti-polio, -meningitis, -difteritis, -tetanica, -pertosse, -rosolia, -HPV, -hepatitis, -influenza, -morbillo, -varicella. The immune response against infections. Aspecifica: inflammation, phagocytes, phagocytosis and killing, complement; Specification: humoral and cell-mediated. Evasion of immune response by microorganisms. Exercises Theoretical (visual experiments) Laboratory diagnosis of infectious diseases (microbiological techniques): plating of bacteria on solid media (selective, differential, selective / differential); Gram and Ziehl-Neelsen staining; Calculation of plaque forming units (CFU); tests of bacterial viability (XTT / MTT, Live / Dead assay, infrared assay); Biofilm analysis using Scanning Electron Microscopy (SEM); immunofluorescence and immunohistochemistry; fluorescent in situ hybridization (FISH); plaque assay; replica plate; the antibiogram, ELISA, PCR in microbiology. Practices: plating, identification of bacterial species, Gram and Ziehl-Neelsen stains, antibiogram, calculation of CFU and evaluation of bacterial metabolic activity by XTT.e assay. For the Medical Immunology contents see the respective Professor page
Reference textsMedical Microbiology recommended texts: - Principi di Microbiologia medica. Casa Editrice Ambrosiana. Antonelli, Clementi, Pozzi, Rossolini, III edizione - Microbiologia medica. Piccin, Carrol, Morse, Mietzner, Miller, ventisettesima edizione. For the medical Immunology texts see the respective Professor page
Teaching targetsAt the end of the course the students must be able to reach: Specific training objectives that can be measured and reachable within the foreseen time frame; microbiology and immunology are disciplines in continuous development, sciences in which other disciplines such as molecular biology, chemistry and biochemistry, genetics, etc. converge. The course aims to provide students with the opportunity to make connections between disciplines and elements sufficient to understand the biology of the main microorganismsms, microbial habitats and immune response. The course aims to train students on the different aspects of general and medical microbiology, with particular reference to the structural and molecular organization and to the functions of prokaryotic, eukaryotic and virus organisms and their pathogenetic mechanisms. The student will acquire theoretical knowledge about the general and special characters of prokaryotic microorganisms (bacteria), eukaryotes (fungi and protozoa) and viruses. The course provides students with in-depth knowledge of the world of microorganisms so as to understand the positive and harmful interactions between microorganism and man. The course also provides theoretical and practical knowledge of the basic methodologies of the microbiological laboratory to allow the student the elements to choose to use and apply the appropriate methods of analysis for the diagnosis of infectious diseases. The course presents an in-depth study on the issues related to the relationship between the human body and microorganisms, using a medical perspective. Infections caused by some important human pathogens will be analyzed, with particular reference to the molecular mechanisms involved. Finally, the main diagnostic techniques used to identify the most important pathogens will be presented For the specific objectives of the Immunology course see the respective professor page
Prerequisitesfor the understanding of the course, students must be possess a minimum set of basic principles of cellular biology, chemistry, genetic, immunology and microbiology (minimal knowledge). They must also possess an English knowledge at level B1. Specific prerequisites are not foreseen.
Didattics MethodsThe educational material is uploaded on the site https://www.dir.uniupo.it/ The teaching methods used by the course are represented by: - Presentations in MS-Power Point format - Screening of Peer Reviewed Scientific Video Journals (JoVE, Microbiology) - Use of the fast and fun "Kahoot" learning platform useful for ongoing assessment of learning (Microbiology) - Laboratory theoretical-practical exercises to encourage students in their role as active participants (Microbiology) The Microbiology course of 5 total CFU (44 h) is organized as follows: - 32 hours of theoretical lectures, conducted in the classroom using power-point slides, classic blackboard and Kahoot platform, on all course topics. These lessons include a continuous direct examination in the classroom of the level of understanding of the various topics dealt with through dialogue, questions and kahoot in order to stimulate motivation, curiosity, critical spirit, autonomy and involvement in the learning processes of students. - 12 hours of theoretical-practical exercises (of which 6 in the form of visual experiments in the classroom and 6 in the didactic laboratory;these ones replicated in groups of about 25 people). These exercises allow students to put into practice and therefore apply the main microbiological issues and methodologies addressed in class. For practical exercises students are divided into groups of about 25 people. The Immunology course of 5 total CFU (40 h of theoretical lectures)
Other informationsEmail Microbiology: A+B gropus: barbara.azzimonti@med.uniupo.it Email Immunology: A group: umberto.dianzani@med.uniupo.it; B group: annalisa.chiocchetti@med.uniupo.it The Profs are available to discuss any doubts concerning the topics of the course. The Profs receive the students, by appointment, in their study c / o Palazzo Bellini, Department of Health Sciences, Via Solaroli 17, 28100 Novara. At the end of the course the exam will be simulated
Grading rulesSince the number of students is very high (groups A + B), the final evaluation test consists of a Microbiology written exam followed by an oral part for Immunology, on the pre-established dates of the exams, as follows: - Twenty multiple choice questions (more correct answer options; you must correctly answer to all the options to get 0.5 points / question (total 10 points) - Four statements to be indicated as V / F (1.5 points) and motivated in 4-5 lines (4 points), to obtain 5.5 points / question (total 22 points). The sum of the two scores must not be less than 18; the maximum achievable score is 30 cum laude. The questions focus on all the topics developed during the classroom lessons and practical laboratory exercises. A moment will be devoted to the correction of the written exams in which each student will be able to recognize its mistakes and identify the correct answers The Immunology evaluation test is oral. The objective of the exam consists in verifying the level of achievement of the previously indicated educational objectives and in the ability to communicate and critically elaborate in writing and speaking the topics learned in italian with appropriateness of language (correct use of technical-scientific terms). The examination also serves to evaluate the acquisition of knowledge, the ability to organize a structured response with clear clarity, the synthesis and appropriateness of language and the analytical / critical approach of the student towards the concepts learned during the course.
Full argumentsSince the number of students is very high (groups A + B), the final evaluation test consists of a written exam, on the pre-established dates of the exams, as follows: - Twenty multiple choice questions (more correct answer options; you must correctly answer to all the options to get 0.5 points / question (total 10 points) - Four statements to be indicated as V / F (1.5 points) and motivated in 4-5 lines (4 points), to obtain 5.5 points / question (total 22 points). The sum of the two scores must not be less than 18; the maximum achievable score is 30 cum laude. The questions focus on all the topics developed during the classroom lessons and practical laboratory exercises. The objective of the exam consists in verifying the level of achievement of the previously indicated educational objectives and in the ability to communicate and critically elaborate in writing the topics learned in italian language with appropriateness of language (correct use of technical-scientific terms). The examination also serves to evaluate the acquisition of knowledge, the ability to organize a structured response with clear clarity, the synthesis and appropriateness of language and the analytical / critical approach of the student towards the concepts learned during the course. A moment will be devoted to the correction of the written exams in which each student will be able to recognize its mistakes and identify the correct answers The bacterial cell: organization and structure. Division and growth. Bacterial metabolism. Resident microbial flora: the microbiota. Principles of bacterial virulence: Bacterial toxins: exotoxins and endotoxins; the spore; the capsule; the pili and the scourges; enzymes; the siderophores; the bacterial biofilm. Mechanisms of gene transfer: conjugation, transformation, transduction, transposition. Basics of the main pathogenic bacteria: Staphylococci, Streptococci, Enterobacteriaceae, Clostridia, Mycobacteria, Neisseria, Pseudomonas, A. Baumannii, Pneumococci, Chlamydia and Helicobacteria. Viruses: organization and structure, cultivation and multiplication, pathogenicity. Basics of the main pathogenic viruses (these topics will be explored during the Master's Degree in the Course of Molecular Virology): Hepadnavirus and other viruses causing hepatitis, Herpesvirus, Papillomavirus, Orthomyxovirus, Retrovirus. Basics of subviral agents: prions. Basics on eukaryotic microorganisms: the protists. Control of microbial growth: Disinfection and sterilization; antibiotics; natural antimicrobials; antiviral drugs; resistance mechanisms (superbugs). Vaccines: anti-polio, -meningitis, -difteritis, -tetanica, -pertosse, -rosolia, -HPV, -hepatitis, -influenza, -morbillo, -varicella. The immune response against infections. Aspecifica: inflammation, phagocytes, phagocytosis and killing, complement; Specification: humoral and cell-mediated. Evasion of immune response by microorganisms. Exercises Theoretical (visual experiments) Laboratory diagnosis of infectious diseases (microbiological techniques): plating of bacteria on solid media (selective, differential, selective / differential); Gram and Ziehl-Neelsen staining; Calculation of plaque forming units (CFU); tests of bacterial viability (XTT / MTT, Live / Dead assay, infrared assay); Biofilm analysis using Scanning Electron Microscopy (SEM); immunofluorescence and immunohistochemistry; fluorescent in situ hybridization (FISH); plaque assay; replica plate; the antibiogram, ELISA, PCR in microbiology. Practices: plating, identification of bacterial species, Gram and Ziehl-Neelsen stains, antibiogram, calculation of CFU and evaluation of bacterial metabolic activity by XTT.e assay. For the Immunology program see the respective Professor page
Expected learning objectivesLEARNING, KNOWLEDGE AND UNDERSTANDING: At the end of the course, the student should demonstrate knowledge of the structure, substructure and biological properties of bacteria, viruses and fungi and have understood, within the time frame, the main mechanisms underlying the infectious processes cause of human pathology and immunologic response. It should also demonstrate knowledge of the main techniques used in bacteriological and virological diagnostics. ABILITY TO APPLY KNOWLEDGE AND UNDERSTANDING: The student should have acquired skills in the field of medical microbiology and immunology that can be applied in laboratory practice. TRANSVERSAL SKILLS: The student must also be able to have JUDGMENT AUTONOMY and SKILLS to COMMUNICATE and motivate in theory and in practice his / her procedural / experimental choices.
Modules
Course ID Course SSD Teachers
BT029 Immunology MED/04 - Experimental medicine and pathophysiology DIANZANI Umberto, CHIOCCHETTI Annalisa
BT030 Medical microbiology MED/07 - Microbiology and clinical microbiology AZZIMONTI Barbara
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CourseImmunology
Course IDBT029
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersDIANZANI Umberto, CHIOCCHETTI Annalisa
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/04 - Experimental medicine and pathophysiology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CourseImmunology
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderDIANZANI Umberto
TeachersDIANZANI Umberto
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
Abstract-Cells and organs of the immune system. -Antigens-Antibodies. -Complement. -Monoclonal antibodies and laboratory technique. -TCR and MHC. -Antigen presenting cells. -B and T cell maturation. -Cytokines. -Functions of T and NK cells. -Immunological memory. -Immunoelusion. -Passive and active immunization. -Hypersensibility. -Autoimmune diseases.
Reference textsKubi, Immunologia, Utet Murphy, Immunobiologia di Janeway
Teaching targetsTo acquire knowledges on the immune system function at the level of innate and adaptive immunity in order to understand also the mechanisms of immune evasion, vaccination, and the etiology and pathogenesis of allergic and autoimmune diseases.
PrerequisitesKnowledge of basic cell biology and genetic
Didattics MethodsFrontal teaching with slides and written synopsis of the course
Other informationsnone
Grading rulesoral examination
Full arguments-Immune system. Innate and adaptive immunity. -Cells of the immune system. Receptors and mediators of innate immunity. -Organs of the immune system. -Antigens-Antibodies. Structure and function of the antibodies and antibody isotypes. Features of B cell antigens. Aptens. BCR structure and signaling. -Complement. Classic, alternative, lectinic pathway. Receptors and inhibitors. -Monoclonal antibodies and laboratory techniques. Monoclonal and polyclonal antibodies. Immunofluorescence, Western blot, RIA, ELISA, immunodiffusion. -TCR and MHC. Structure and function. The HLA system. Antigen presentation (endocytic, cytosolic, lipid pathways, cross-presentation). CD3, CD4, CD8, function and signaling. -Antigen presenting cells. Cell types, function second signal. CD28, CTLA-4 and PD-1. -B cell repertoire. Ig gene rearrangement. Antigen-independent and antigen-dependent maturation of B cells. -T cell repertoire. Thymic maturation of conventional and regulatory T cells. -Cytokines. Structure, function, receptors and signaling. -Helper and cytotoxic activities aof T cells. Th1, Th2, Th17, natural and induced Treg, CTL, NK, NKT, Tγδ. -Immune evasion. Mechanisms of immune evasion of infectious agents and tumors. -Passive and active immunization. Vaccines. -Type I, II, III, IV hypersensivity. Etiology, pathogenesis, examples. -Autoimmune diseases. Etiology, pathogenesis, examples.
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CourseImmunology
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCHIOCCHETTI Annalisa
TeachersCHIOCCHETTI Annalisa
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
Abstract-Cells and organs of the immune system. -Antigens-Antibodies. -Complement. -Monoclonal antibodies and laboratory technique. -TCR and MHC. -Antigen presenting cells. -B and T cell maturation. -Cytokines. -Functions of T and NK cells. -Immunological memory. -Immunoelusion. -Passive and active immunization. -Hypersensibility. -Autoimmune diseases.
Reference textsKubi, Immunologia, Utet Murphy, Immunobiologia di Janeway
Teaching targetsTo acquire knowledges on the immune system function at the level of innate and adaptive immunity in order to understand also the mechanisms of immune evasion, vaccination, and the etiology and pathogenesis of allergic and autoimmune diseases.
PrerequisitesKnowledge of basic cell biology and genetic
Didattics MethodsFrontal teaching with slides and written synopsis of the course
Other informationsnone
Grading rulesoral examination
Full arguments-Immune system. Innate and adaptive immunity. -Cells of the immune system. Receptors and mediators of innate immunity. -Organs of the immune system. -Antigens-Antibodies. Structure and function of the antibodies and antibody isotypes. Features of B cell antigens. Aptens. BCR structure and signaling. -Complement. Classic, alternative, lectinic pathway. Receptors and inhibitors. -Monoclonal antibodies and laboratory techniques. Monoclonal and polyclonal antibodies. Immunofluorescence, Western blot, RIA, ELISA, immunodiffusion. -TCR and MHC. Structure and function. The HLA system. Antigen presentation (endocytic, cytosolic, lipid pathways, cross-presentation). CD3, CD4, CD8, function and signaling. -Antigen presenting cells. Cell types, function second signal. CD28, CTLA-4 and PD-1. -B cell repertoire. Ig gene rearrangement. Antigen-independent and antigen-dependent maturation of B cells. -T cell repertoire. Thymic maturation of conventional and regulatory T cells. -Cytokines. Structure, function, receptors and signaling. -Helper and cytotoxic activities aof T cells. Th1, Th2, Th17, natural and induced Treg, CTL, NK, NKT, Tγδ. -Immune evasion. Mechanisms of immune evasion of infectious agents and tumors. -Passive and active immunization. Vaccines. -Type I, II, III, IV hypersensivity. Etiology, pathogenesis, examples. -Autoimmune diseases. Etiology, pathogenesis, examples.
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CourseMedical microbiology
Course IDBT030
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderAZZIMONTI Barbara
TeachersAZZIMONTI Barbara
CFU5
Teaching duration (hours)32
Individual study time 81
SSDMED/07 - Microbiology and clinical microbiology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Teaching languageThe course is held in Italian. The power point slides can be in both Italian and English. The projected visual experiments are in English
AbstractConsistent with the specific training objectives of the CdS, reported in the SUA-CdS, framework A4.a, the contents are described below: The bacterial cell: organization and structure. Division and growth. Bacterial metabolism. Resident microbial flora: the microbiota. Principles of bacterial virulence: Bacterial toxins: exotoxins and endotoxins; the spore; the capsule; the pili and the scourges; enzymes; the siderophores; the bacterial biofilm. Mechanisms of gene transfer: conjugation, transformation, transduction, transposition. Basics of the main pathogenic bacteria: Staphylococci, Streptococci, Enterobacteriaceae, Clostridia, Mycobacteria, Neisseria, Pseudomonas, A. Baumannii, Pneumococci, Chlamydia and Helicobacteria. Viruses: organization and structure, cultivation and multiplication, pathogenicity. Basics of the main pathogenic viruses (these topics will be explored during the Master's Degree in the Course of Molecular Virology): Hepadnavirus and other viruses causing hepatitis, Herpesvirus, Papillomavirus, Orthomyxovirus, Retrovirus. Basics of subviral agents: prions. Basics on eukaryotic microorganisms: the protists. Control of microbial growth: Disinfection and sterilization; antibiotics; natural antimicrobials; antiviral drugs; resistance mechanisms (superbugs). Vaccines: anti-polio, -meningitis, -difteritis, -tetanica, -pertosse, -rosolia, -HPV, -hepatitis, -influenza, -morbillo, -varicella. The immune response against infections. Aspecifica: inflammation, phagocytes, phagocytosis and killing, complement; Specification: humoral and cell-mediated. Evasion of immune response by microorganisms. Exercises Theoretical (visual experiments) Laboratory diagnosis of infectious diseases (microbiological techniques): plating of bacteria on solid media (selective, differential, selective / differential); Gram and Ziehl-Neelsen staining; Calculation of plaque forming units (CFU); tests of bacterial viability (XTT / MTT, Live / Dead assay, infrared assay); Biofilm analysis using Scanning Electron Microscopy (SEM); immunofluorescence and immunohistochemistry; fluorescent in situ hybridization (FISH); plaque assay; replica plate; the antibiogram, ELISA, PCR in microbiology. Practices: plating, identification of bacterial species, Gram and Ziehl-Neelsen stains, antibiogram, calculation of CFU and evaluation of bacterial metabolic activity by XTT.e assay
Reference textsRecommended texts: - Principi di Microbiologia medica. Casa Editrice Ambrosiana. Antonelli, Clementi, Pozzi, Rossolini, III edizione - Microbiologia medica. Piccin, Carrol, Morse, Mietzner, Miller, ventisettesima edizione
Teaching targetsAt the end of the course the students must be able to reach: Specific training objectives that can be measured and reachable within the foreseen time frame; microbiology is a discipline in continuous development, a science in which other disciplines such as molecular biology, chemistry and biochemistry, genetics, etc. converge. The course aims to provide students with the opportunity to make connections between disciplines and elements sufficient to understand the biology of the main microorganisms and microbial habitats. The course aims to train students on the different aspects of general and medical microbiology, with particular reference to the structural and molecular organization and to the functions of prokaryotic, eukaryotic and virus organisms and their pathogenetic mechanisms. The student will acquire theoretical knowledge about the general and special characters of prokaryotic microorganisms (bacteria), eukaryotes (fungi and protozoa) and viruses. The course provides students with in-depth knowledge of the world of microorganisms so as to understand the positive and harmful interactions between microorganism and man. The course also provides theoretical and practical knowledge of the basic methodologies of the microbiological laboratory to allow the student the elements to choose to use and apply the appropriate methods of analysis for the diagnosis of infectious diseases. The course presents an in-depth study on the issues related to the relationship between the human body and microorganisms, using a medical perspective. Infections caused by some important human pathogens will be analyzed, with particular reference to the molecular mechanisms involved. Finally, the main diagnostic techniques used to identify the most important pathogens will be presented.
Prerequisitesfor the understanding of the course, students must be possess a minimum set of basic principles of cellular biology, chemistry, genetic, immunology and microbiology (minimal knowledge). They must also possess an English knowledge at level B1. Specific prerequisites are not foreseen.
Didattics MethodsAll the educational material is uploaded on the site https://www.dir.uniupo.it/ The teaching methods used by the course are represented by: - Presentations in MS-Power Point format - Screening of Peer Reviewed Scientific Video Journals (JoVE) - Use of the fast and fun "Kahoot" learning platform useful for ongoing assessment of learning - Laboratory theoretical-practical exercises to encourage students in their role as active participants The course of 5 total CFU (44 h) is organized as follows: - 32 hours of theoretical lectures, conducted in the classroom using power-point slides, classic blackboard and Kahoot platform, on all course topics. These lessons include a continuous direct examination in the classroom of the level of understanding of the various topics dealt with through dialogue, questions and kahoot in order to stimulate motivation, curiosity, critical spirit, autonomy and involvement in the learning processes of students. - 12 hours of theoretical-practical exercises (of which 6 in the form of visual experiments in the classroom and 6 in the didactic laboratory;these ones replicated in groups of about 25 people). These exercises allow students to put into practice and therefore apply the main microbiological issues and methodologies addressed in class. For practical exercises students are divided into groups of about 25 people.
Other informationsEmail: barbara.azzimonti@med.uniupo.it The teacher, at the end of the lessons or after contacting email, is available to discuss any doubts concerning the topics of the course. The teacher receives the students, by appointment, in his study c / o Palazzo Bellini, Department of Health Sciences, Via Solaroli 17, 28100 Novara. At the end of the course the exam will be simulated
Grading rulesSince the number of students is very high (groups A + B), the final evaluation test consists of a written exam, on the pre-established dates of the exams, as follows: - Twenty multiple choice questions (more correct answer options; you must correctly answer to all the options to get 0.5 points / question (total 10 points) - Four statements to be indicated as V / F (1.5 points) and motivated in 4-5 lines (4 points), to obtain 5.5 points / question (total 22 points). The sum of the two scores must not be less than 18; the maximum achievable score is 30 cum laude. The questions focus on all the topics developed during the classroom lessons and practical laboratory exercises. The objective of the exam consists in verifying the level of achievement of the previously indicated educational objectives and in the ability to communicate and critically elaborate in writing the topics learned in italian language with appropriateness of language (correct use of technical-scientific terms). The examination also serves to evaluate the acquisition of knowledge, the ability to organize a structured response with clear clarity, the synthesis and appropriateness of language and the analytical / critical approach of the student towards the concepts learned during the course. A moment will be devoted to the correction of the written exams in which each student will be able to recognize its mistakes and identify the correct answers
Full argumentsThe bacterial cell: organization and structure. Division and growth. Bacterial metabolism. Resident microbial flora: the microbiota. Principles of bacterial virulence: Bacterial toxins: exotoxins and endotoxins; the spore; the capsule; the pili and the scourges; enzymes; the siderophores; the bacterial biofilm. Mechanisms of gene transfer: conjugation, transformation, transduction, transposition. Basics of the main pathogenic bacteria: Staphylococci, Streptococci, Enterobacteriaceae, Clostridia, Mycobacteria, Neisseria, Pseudomonas, A. Baumannii, Pneumococci, Chlamydia and Helicobacteria. Viruses: organization and structure, cultivation and multiplication, pathogenicity. Basics of the main pathogenic viruses (these topics will be explored during the Master's Degree in the Course of Molecular Virology): Hepadnavirus and other viruses causing hepatitis, Herpesvirus, Papillomavirus, Orthomyxovirus, Retrovirus. Basics of subviral agents: prions. Basics on eukaryotic microorganisms: the protists. Control of microbial growth: Disinfection and sterilization; antibiotics; natural antimicrobials; antiviral drugs; resistance mechanisms (superbugs). Vaccines: anti-polio, -meningitis, -difteritis, -tetanica, -pertosse, -rosolia, -HPV, -hepatitis, -influenza, -morbillo, -varicella. The immune response against infections. Aspecifica: inflammation, phagocytes, phagocytosis and killing, complement; Specification: humoral and cell-mediated. Evasion of immune response by microorganisms. Exercises Theoretical (visual experiments) Laboratory diagnosis of infectious diseases (microbiological techniques): plating of bacteria on solid media (selective, differential, selective / differential); Gram and Ziehl-Neelsen staining; Calculation of plaque forming units (CFU); tests of bacterial viability (XTT / MTT, Live / Dead assay, infrared assay); Biofilm analysis using Scanning Electron Microscopy (SEM); immunofluorescence and immunohistochemistry; fluorescent in situ hybridization (FISH); plaque assay; replica plate; the antibiogram, ELISA, PCR in microbiology. Practices: plating, identification of bacterial species, Gram and Ziehl-Neelsen stains, antibiogram, calculation of CFU and evaluation of bacterial metabolic activity by XTT.e assay
Expected learning objectivesLEARNING, KNOWLEDGE AND UNDERSTANDING: At the end of the course, the student should demonstrate knowledge of the structure, substructure and biological properties of bacteria, viruses and fungi and have understood, within the time frame, the main mechanisms underlying the infectious processes cause of human pathology. It should also demonstrate knowledge of the main techniques used in bacteriological and virological diagnostics. ABILITY TO APPLY KNOWLEDGE AND UNDERSTANDING: The student should have acquired skills in the field of medical microbiology that can be applied in laboratory practice. TRANSVERSAL SKILLS: The student must also be able to have JUDGMENT AUTONOMY and SKILLS to COMMUNICATE and motivate in writing and in practice his / her procedural / experimental choices.
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CourseGenetics
Course IDMS0443
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersGIORDANO Mara, PERSICHETTI Francesca, CORRADO Lucia, BARIZZONE Nadia
CFU6
Teaching duration (hours)50
Individual study time 98
SSDMED/03 - Medical genetics
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseGenetics
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGIORDANO Mara
TeachersGIORDANO Mara, PERSICHETTI Francesca
Course typeGruppi
Year2
PartitionGruppo A
Abstract-Structure and evolution of the human genome. - Cell division: mitosis and meiosis. -Correlation genotype-phenotype (example: ABO blood group, sickle-cell anemia). -Principles of Mendelian genetics. - Exceptions to the laws of Mendel. - Chromosomal classification criteria and chromosomal aberrations - Population genetics. -Transmission of independent and concatenated characters. Linkage analysis. - Quantitative and semi-quantitative multifactorial traits. - Methods of molecular genetics - Analysis of genomic databases
Reference textsEredità, Principi e problematiche della genetica umana, M.R. Cummings, Edizioni EdiSES, II edizione (2009) Genetica, Peter J. Russel, Edizioni EdiSES, II edizione
Teaching targetsTo understand the relationships between genetic information and the phenotype. To Know how to evaluate the risk of recurrence of genetic diseases or with genetic components in families. To know the molecular basis of genetically based diseases. Apply knowledge to the analysis and resolution of problems of formal and molecular genetics. To apply the basic methods of molecular genetics such as PCR and sequencing through practical laboratory activities
PrerequisitesFundamentals of biochemistry, cell biology and statistics.
Didattics MethodsLectures with presentations in MS-Power Point format. Assignment of problems od genetics to be solved in class with relative discussion Practical laboratory on PCR and DNA sequencing
Grading rulesThe knowledge will be verified by written exam with open and / or multiple choice questions. For most questions, the answer will require the solving of problems
Full arguments-DNA structure. Organization of DNA in chromosomes. Structure and function of the chromosome. -The Human Genome structure. Organization of genes. Transposable elements. Ripetitive DNA sequences. Gene families. -Cell division: mitosis and meiosis. -Genotype-phenotype correlation (example: ABO blood group, sickle cell anemia). Protein translation and genetic code. Correlation between type of mutations and dominant or recessive phenotype at different levels of phenotype investigation. Genetic consequences of meiosis. -Principles of Mendelian genetics. Transmission in families of autosomal recessive, dominant and X-linked monofactorial traits; recurrence risks in families. -"Exceptions" to Mendel’s rules. Incomplete penetrance, variable expressivity, genetic heterogeneity. X-chromosome inactivation, mitochondrial inheritance, genomic imprinting -Criteria for classification of chromosomes and methods for their identification. Chromosomal aberrations and their incidence at birth; Reproductive risks associated with chromosomal aberrations. -Population genetics. Gene and genotypic frequencies in the population and Hardy-Weinberg equilibrium. -Evolution of human populations. Evolutionary forces (mutation, selection, genetic drift, migration) that influence allelic and genotype frequencies. -Transmission of independent and concatenated characters. Localization of genes on chromosomes based on their transmission in families (linkage analysis). Different methods for gene mapping. -Multi-factorial characters. Population distribution of multifactorial characters; evaluation of the weight of the genetic component in multifactorial disorders; identification of susceptibility genes in multifactorial diseases. -DNA investigation methods. Polymerase Chain Reaction (PCR); Analysis of VNTR, STR and fingerprint polymorphisms; DNA sequencing; Methods for the identification of nucleotide variations. Genomic analysis through next generation sequencing methods and chromosomal microarray (MCA). -Analysis of Genomic Databases
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CourseGenetics
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCORRADO Lucia
TeachersCORRADO Lucia, BARIZZONE Nadia, PERSICHETTI Francesca
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
AbstractThe module aims to teach the student the knowledge and understanding of the transmission of linked loci, of the concept of Linkage Disequilibrium and of the methods for the study of complex diseases.
Reference textsEredità, Principi e problematiche della genetica umana, M.R. Cummings, Edizioni EdiSES, II edizione (2009) Genetica, Peter J. Russel, Edizioni EdiSES, II edizione Genetica Umana e Medica Giovanni Neri e Maurizio Genuardi Edizioni EDRA Masson 2014 (III edizione)
Teaching targetsThe module aims to teach the student the knowledge and understanding of the transmission of linked loci, of the concept of Linkage Disequilibrium and of the methods for the study of complex diseases, and to make the student able to understand the utility, limitations and critical aspects of the methods for the study of complex diseases, particularly of the GWASs.
PrerequisitesFundamentals of biochemistry, cell biology and statistics.
Didattics MethodsLectures with presentations in MS-Power Point format.
Grading rulesMultiple choice quiz
Full argumentsGenetic recombination. Transmission of linked loci. Frequency of recombination and genetic maps. Linkage analysis. Linkage Disequilibrium. Complex traits. Genetic methods for the study of complex diseases. Association studies. Genome Wide Association Studies (GWAS).
Expected learning objectivesKnowledge and understanding of the transmission of linked loci, of the concept of Linkage Disequilibrium and of the methods for the study of complex diseases. Applying knowledge and understanding: At the end of the course the student should be able to apply the acquired knowledge to solve questions regarding the topics of the module. Making judgements. Ability to understand the utility, limitations and critical aspects of the methods for the study of complex diseases, particularly of the GWASs. Communication skills Ability to expose the subjects of the course in a clear and comprehensible way and with the use of the appropriate scientific terminology. Learning skills Ability to deepen his knowledge of the genetics on several books and on scientific articles in order to follow with a critical attitude the advancement of discipline.
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CourseHuman physiology
Course IDBT035
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersGROSSINI Elena, LIM Dmitry
CFU5
Teaching duration (hours)40
Individual study time 85
SSDBIO/09 - Physiology
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodSecondo Semestre
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseHuman physiology
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGROSSINI Elena
TeachersGROSSINI Elena, LIM Dmitry
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
AbstractThe main focus of the course of Physiology is to achieve the knowledge of principles at basis of functional activity of various organs.The cardiovascular, respiratory, renal, digestive and endocrine systems will be treated. As regarding the nervous system, its role in the regularion of above systems will be treated.
Reference textsFisiologia Umana by F. Grassi, D. Negrini and CA Porro, Poletto Editore
Teaching targetsThe educational objectives are addressed to the knowledge of principles governing the functional acvitity of various organs and apparatus and to match them with main cellular mechanisms.
PrerequisitesStudents should be in possession of basic knowledge of Anatomy, Istology, Chemistry and Biochemistry.
Didattics MethodsMS-Power point slides
Grading rulesWritten test (true/false) on the entire programme
Full argumentsCardiovascular system: properties of the myocardium, cardiac cycle, cardiac output, arterial blood pressure. Respiratory system:respiratory mechanic; alveolar ventilation, alveolar gas exchange, chemical and nervous respiratory control, blood gas transport. Renal system:glomerular filtration, re-absorbption and secretion mechanisms, renal clearances. Digestive system: composition, production and role of digestive secretes, intestinal absorbption, liver function. Endocrine system: physiologic functions of hormones released by hypothalamus, pituitary gland, thyroid, parathyroid glands, surrenal glands, endocrine pancreas and gonades. Nervous system: the neurons
Expected learning objectivesThe student should: (KNOWLEDGE) -be aware of basic physiologic mechanisms that regulate the function of organs (COMPETENCE) -use the knowledge for solving main biotechnologic issues (TRANSVERSAL ABILITY) -to be able to read, understand and discuss technical material taken from books, other tests etc; -to know or find out the international terminology
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CourseHuman physiology
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGROSSINI Elena
TeachersGROSSINI Elena, LIM Dmitry
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
AbstractThe main focus of the course of Physiology is to achieve the knowledge of principles at basis of functional activity of various organs.The cardiovascular, respiratory, renal, digestive and endocrine systems will be treated. As regarding the nervous system, its role in the regularion of above systems will be treated.
Reference textsFisiologia Umana by F. Grassi, D. Negrini and CA Porro, Poletto Editore
Teaching targetsThe educational objectives are addressed to the knowledge of principles governing the functional acvitity of various organs and apparatus and to match them with main cellular mechanisms.
PrerequisitesStudents should be in possession of basic knowledge of Anatomy, Istology, Chemistry and Biochemistry.
Didattics MethodsMS-Power point slides
Grading rulesWritten test (true/false) on the entire programme
Full argumentsCardiovascular system: properties of the myocardium, cardiac cycle, cardiac output, arterial blood pressure. Respiratory system:respiratory mechanic; alveolar ventilation, alveolar gas exchange, chemical and nervous respiratory control, blood gas transport. Renal system:glomerular filtration, re-absorbption and secretion mechanisms, renal clearances. Digestive system: composition, production and role of digestive secretes, intestinal absorbption, liver function. Endocrine system: physiologic functions of hormones released by hypothalamus, pituitary gland, thyroid, parathyroid glands, surrenal glands, endocrine pancreas and gonades. Nervous system. The neurons
Expected learning objectivesThe student should: (KNOWLEDGE) -be aware of basic physiologic mechanisms that regulate the function of organs (COMPETENCE) -use the knowledge for solving main biotechnologic issues (TRANSVERSAL ABILITY) -to be able to read, understand and discuss technical material taken from books, other tests etc; -to know or find out the internationl terminology
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CourseLaboratory cell cultures
Course IDBT031
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMERLIN Simone
TeachersBOLDORINI Renzo Luciano, MERLIN Simone, BORSOTTI Chiara
CFU6
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year2
PeriodPrimo Semestre
SiteNOVARA
Grading typeFinal grade
Modules
Course ID Course SSD Teachers
MS0230 Histopathological preparations MED/08 - Pathology BOLDORINI Renzo Luciano
MS0445 Laboratory cell cultures BIO/17 - Histology MERLIN Simone, BORSOTTI Chiara
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CourseHistopathological preparations
Course IDMS0230
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBOLDORINI Renzo Luciano
TeachersBOLDORINI Renzo Luciano
CFU1
Teaching duration (hours)8
Individual study time 28
SSDMED/08 - Pathology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year2
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Teaching languageItalian
AbstractSpecifiche modalità di trattamento dei materiali biologici, cito ed istologici, significato dell’esame intraoperatorio. Principi e tecniche di estrazione degli acidi nucleici, metodiche di amplificazione genica, tecniche e principi di analisi di sequenza genica. Principi di colorazioni istochimiche ed immuno-istochimiche e loro utilizzo in ambito diagnostico.
Reference textsGallo P. - Anatomia Patologica per lauree triennali UTET
Teaching targetsConoscere i principi e le principali metodologie tecniche nella diagnostica autoptica, istologica e citologica in Anatomia Patologica. Comprendere le specifiche tecniche del trattameto degli acidi nucleici nella peculiarità di un laboratorio di Anatomia Patologica.
PrerequisitesConoscenze di base di chimica, anatomia ed istologia
Didattics MethodsPresentazioni in formato power-point, CD-ROM interattivi e siti internet didattici.
Grading rulesTest scritto a domande aperte o quiz a risposta multipla.
Full argumentsPrincipi di diagnostica macro e microscopica, modalità di preparazione tecnica dei materiali citologici ed istologici; procedure tecniche e finalità delle colorazioni istologiche, istochimiche, immunoistochimiche e della diagnostica ultrastrutturale. Metodiche di conservazione degli acidi nucleici a fini diagnostici.
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CourseLaboratory cell cultures
Course IDMS0445
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersMERLIN Simone, BORSOTTI Chiara
CFU5
Teaching duration (hours)10
Individual study time 69
SSDBIO/17 - Histology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CourseLaboratory cell cultures
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMERLIN Simone
TeachersMERLIN Simone
Course typeGruppi
Year2
PartitionGruppo A
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CourseLaboratory cell cultures
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBORSOTTI Chiara
TeachersBORSOTTI Chiara
Course typeGruppi
Year2
PartitionGruppo B
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CourseMolecular Biology
Course IDBT022
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderSANTORO Claudio Ventura
TeachersPELICCI Giuliana, CORA' Davide, SANTORO Claudio Ventura, CORAZZARI Marco
CFU11
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year2
PeriodPrimo Semestre
SiteNOVARA
Grading typeFinal grade
Teaching languageITALIAN
AbstractTHOSE PROVIDED BY EACH MODULE
Reference textsSEE THOSE SUGGESTED BY EACH MODULE
Teaching targetsTHOSE PROVIDED BY EACH MODULE
PrerequisitesAS ABOVE
Didattics MethodsAS ABOVE
Other informationsNONE
Grading rulesA SINGLE EXAM WITH MODALITIES INDICATED IN EACH MODULE
Full argumentsAS REPORTED BY EACH MODULE
Expected learning objectivesAs reported by each module
Modules
Course ID Course SSD Teachers
BT071 Biochemistry and molecular biology BIO/11 - Molecular biology PELICCI Giuliana, CORA' Davide
BT023 Recombinant technology lab BIO/13 - Experimental biology SANTORO Claudio Ventura, CORAZZARI Marco
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CourseBiochemistry and molecular biology
Course IDBT071
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersPELICCI Giuliana, CORA' Davide
CFU6
Teaching duration (hours)48
Individual study time 102
SSDBIO/11 - Molecular biology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CourseBiochemistry and molecular biology
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPELICCI Giuliana
TeachersPELICCI Giuliana
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
Abstract- Introduction to molecular biology - Epigenetics and molecular biology: chromatin structure and regulation - Regulation of gene expression: transcription - mRNA processing and post-transcriptional regulation - microRNAs and Short-interferingRNAs - DNA repair - DNA replication - Cell cycle regulation - Cell response to stress - Apoptosis - Genetic manipulations in mice
Reference textsAlberts et al. “Molecular biology of the Cell” VI E Watson et al. "Molecular biology of the gene" VII E Lewin “Genes X”
Teaching targetsProvide basic knowledge of molecular biology with a focus for eukaryotic organisms and with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.
PrerequisitesLo studente deve conoscere i meccanismi molecolari e cellulari che regolano la replicazione, la crescita cellulare e il programma di espressione genica in organismi eucarioti con alcuni parallelismi negli organismi procarioti.
Didattics MethodsPowerPoint presentations of lessons and articles provided by the lecturer
Other informationsNone
Grading rulesMultiple-choice written exam
Full argumentsOutline - Introduction to molecular biology. DNA structure, role of DNA as genetic material. - Genomics, complexity and gene regulation. - Comparative genomics. Homologous, paralogous and orthologous genes. - Gene Families (e.g. globin, tubulin); evolution and gene duplication. Molecular basis of epigenetics: chromatin structure and regulation. - structure of nucleosomes and chromatin organization. - Histones and their modifications (acetylation, methylation). - Mechanisms of histone remodeling and chromatin modifications and their role in the regulation of gene expression. Bromodomains and chromodomains. Role and examples of histone modifier enzymes: histone acetyl transferase (HAT), histone deacetylase. Histone remodeling complexes. - Insulators - DNA metilation: biological significance, DNA metil-transferase, the role of DNA methylation in regulating gene expression. Gene expression regulation: transcription - differences between transcription in prokaryotes and eukaryotes Transcription and gene expression regulation in eukaryotes: RNA polymerase II, promoter structure, basal transcription factors, Pol II and formation of the initiation complex. The role of the transcription initiation complex. - Recognition of transcription start sites: TATA box the transcription initiation complexes. - Transcription regulatory sequences and transcription factors (modular and dimeric organization of transcription factors). - Interactions between transcription factors and chromatin and remodeling complexes. - Mechanisms for transcription repression - Regulatory transcription activator strategies. Examples (NF-kB) - The main four structural classes of transcription factors: helix-loop-helix, helix-turn-helix (Myc / MAx / Mad), leucine zipper (Jun-Fos, AP1), zinc fingers (steroid hormone receptors). For each class: structural elements and interaction mechanisms with DNA; functional regulation; functions and regulated genes. RNA processing and post-transcriptional control - Meaning of capping and poly-adenylation of transcripts. Capping mechanisms polyadenylation and termination of mRNA. - The meaning of splicing. - Spliceosome and molecular mechanisms of splicing. Alternative splicing. - Splicing site recognition elements: ESE / ISE and ESS / ISS sequences. The role of SR proteins (containing RRS domains) and hRNPs proteins in splicing regulation. - Examples of pathologies caused by mutations affecting splicing. - mRNA editing mechanisms. - Regulation of mRNA transport and localization. - RNA stability (the IRE sequences for the control of stability and translation for mRNAs involved in the Transferrin and Ferritin Receptor). - MicroRNA: genomic structure, transcription and processing, the Dicer complex. - RISC complex, microRNA-mediated mechanisms for regulating gene expression (mRNA stability and translation regulation). Combinatorial nature of the interactions between microRNA and target genes. Examples. - Impact of microRNA discovery in understanding gene functions. Involvement in tumors. Clinical perspectives. - Short-interfering RNAs; basic research usage and clinical implications. DNA Repair Systems - DNA damage repair by base excision, repair of DNA replication errors, repair of DNA breaks on both filaments. DNA Replication - replication mechanism in eukaryotes. Telomeres and telomeres replication. Mechanism controlling cell cycle replication. Cell Cycle Regulation - General principles of cell cycle control. - Role of cyclin / Cdk complexes in cell cycle progression. - Molecular mechanisms of Cdk regulation: interaction with cyclins, activating phosphorylation and inhibiion, interaction with inhibitory proteins (p21, p16, p27 etc.). Mechanisms of regulation of cyclines: transcription, ubiquitination / degradation. - The role of the various cyclin / cdk complexes in the progression of the different phases of the cycle and the concept of "Checkpoint". - Regulation of cyclin / cdk complexes in G1 and S phase: activation of Jun / Fos and Myc and of Myc target genes; regulation of E2F1 by Cyclin D / Cdk4, Rb and cyclin E / Cdk2; Regulation of the cell cycle by tumor suppressors: the p21 family, p16, p27. The regulatory protein p53. Cellular response to stress - The response to DNA damage: transducers and effector complexes: Atm, ChK1 / 2, Cdc25 and p53 in the signal transduction cascade activated by DNA damage. - p53: structure, regulation (from Mdm2, Arf and phosphorylation via Atm / Chk) and function in response DNA damage, cellular senescence, and hypoxia response. Role and mechanisms through p53 promotes cell cycle arrest and apoptosis. Meaning of p53 mutations in tumors. - Cell transformation, general concepts for oncogenesis. Oncogenes and Proto-Oncogenes, fefinition and function. Mechanisms of activation of oncogenes (gain of function). Some examples of oncogenes (tyrosine kinases receptors, cytoplasmic kinase tyrosine, src, bcr-abl, RAS). Cancer suppressors (loss of function); loss of heterozygosity. Definition of caretaker and gatekeeper. Rb; cell cycle inhibitors; p53. - The role of apoptosis in cellular homeostasis. - Extrinsic way for apoptosis activation: pro-apoptotic receptors, death domain (DD) and death effector domain (DED) and the transduction of the apoptotic signal up to the activation of caspases regulators (caspase 8). - Caspase activation mechanism (caspase 3) and the role of their substrates in determining apoptosis (shape change, fragmentation of DNA, phosphoididylserine) and recognition of cells with phagocytic activity. - Intrinsic way for apoptosis activation: central role of regulation of permeability of external mitochondrial membrane in determining the release of cytochrome C, Smac / Diablo and other proteins which regulate apoptosis. Activation of caspases 9 by cytochrome c. Role for the IAP proteins (caspase inhibitors) in apoptosis regulation. - Different regulatory mechanisms for the 3 major caspase families (caspases 8, 9 and 3) - External mitochondrial membrane pore formation: pro-apoptotic proteins Bcl2, proteins Bcl2 and anti-apoptotic one. Functional Meaning of Domains BH1, BH2e BH3. - Necrosis: distinctive features from apoptosis Genetic Manipulations in the Mouse - Standard transgenesis, gene targeting, Cre-Lox systems (constituent vs inducible systems). Examples of mice transgenic or knock-out genes for the study of pathologies (Alzheimer's disease, p53 - / -)
Expected learning objectivesThe student must know the molecular and cellular mechanisms regulating replication, cell growth and the gene expression program in eukaryotic organisms with parallels in prokaryotic organisms.
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CourseBiochemistry and molecular biology
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCORA' Davide
TeachersCORA' Davide
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
Abstract- Introduction to molecular biology - Epigenetics and molecular biology: chromatin structure and regulation - Regulation of gene expression: transcription - mRNA processing and post-transcriptional regulation - microRNAs and Short-interferingRNAs - DNA repair - DNA replication - Cell cycle regulation - Cell response to stress - Apoptosis - Genetic manipulations in mice
Reference textsAlberts et al. “Molecular biology of the Cell” VI E Watson et al. "Molecular biology of the gene" VII E Lewin “Genes X”
Teaching targetsProvide basic knowledge of molecular biology with a focus for eukaryotic organisms and with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.
Didattics MethodsPowerPoint presentations of lessons and articles provided by the lecturer
Grading rulesMultiple-choice written exam
Full argumentsOutline - Introduction to molecular biology. DNA structure, role of DNA as genetic material. - Genomics, complexity and gene regulation. - Comparative genomics. Homologous, paralogous and orthologous genes. - Gene Families (e.g. globin, tubulin); evolution and gene duplication. Molecular basis of epigenetics: chromatin structure and regulation. - structure of nucleosomes and chromatin organization. - Histones and their modifications (acetylation, methylation). - Mechanisms of histone remodeling and chromatin modifications and their role in the regulation of gene expression. Bromodomains and chromodomains. Role and examples of histone modifier enzymes: histone acetyl transferase (HAT), histone deacetylase. Histone remodeling complexes. - Insulators - DNA metilation: biological significance, DNA metil-transferase, the role of DNA methylation in regulating gene expression. Gene expression regulation: transcription - differences between transcription in prokaryotes and eukaryotes Transcription and gene expression regulation in eukaryotes: RNA polymerase II, promoter structure, basal transcription factors, Pol II and formation of the initiation complex. The role of the transcription initiation complex. - Recognition of transcription start sites: TATA box the transcription initiation complexes. - Transcription regulatory sequences and transcription factors (modular and dimeric organization of transcription factors). - Interactions between transcription factors and chromatin and remodeling complexes. - Mechanisms for transcription repression - Regulatory transcription activator strategies. Examples (NF-kB) - The main four structural classes of transcription factors: helix-loop-helix, helix-turn-helix (Myc / MAx / Mad), leucine zipper (Jun-Fos, AP1), zinc fingers (steroid hormone receptors). For each class: structural elements and interaction mechanisms with DNA; functional regulation; functions and regulated genes. RNA processing and post-transcriptional control - Meaning of capping and poly-adenylation of transcripts. Capping mechanisms polyadenylation and termination of mRNA. - The meaning of splicing. - Spliceosome and molecular mechanisms of splicing. Alternative splicing. - Splicing site recognition elements: ESE / ISE and ESS / ISS sequences. The role of SR proteins (containing RRS domains) and hRNPs proteins in splicing regulation. - Examples of pathologies caused by mutations affecting splicing. - mRNA editing mechanisms. - Regulation of mRNA transport and localization. - RNA stability (the IRE sequences for the control of stability and translation for mRNAs involved in the Transferrin and Ferritin Receptor). - MicroRNA: genomic structure, transcription and processing, the Dicer complex. - RISC complex, microRNA-mediated mechanisms for regulating gene expression (mRNA stability and translation regulation). Combinatorial nature of the interactions between microRNA and target genes. Examples. - Impact of microRNA discovery in understanding gene functions. Involvement in tumors. Clinical perspectives. - Short-interfering RNAs; basic research usage and clinical implications. DNA Repair Systems - DNA damage repair by base excision, repair of DNA replication errors, repair of DNA breaks on both filaments. DNA Replication - replication mechanism in eukaryotes. Telomeres and telomeres replication. Mechanism controlling cell cycle replication. Cell Cycle Regulation - General principles of cell cycle control. - Role of cyclin / Cdk complexes in cell cycle progression. - Molecular mechanisms of Cdk regulation: interaction with cyclins, activating phosphorylation and inhibiion, interaction with inhibitory proteins (p21, p16, p27 etc.). Mechanisms of regulation of cyclines: transcription, ubiquitination / degradation. - The role of the various cyclin / cdk complexes in the progression of the different phases of the cycle and the concept of "Checkpoint". - Regulation of cyclin / cdk complexes in G1 and S phase: activation of Jun / Fos and Myc and of Myc target genes; regulation of E2F1 by Cyclin D / Cdk4, Rb and cyclin E / Cdk2; Regulation of the cell cycle by tumor suppressors: the p21 family, p16, p27. The regulatory protein p53. Cellular response to stress - The response to DNA damage: transducers and effector complexes: Atm, ChK1 / 2, Cdc25 and p53 in the signal transduction cascade activated by DNA damage. - p53: structure, regulation (from Mdm2, Arf and phosphorylation via Atm / Chk) and function in response DNA damage, cellular senescence, and hypoxia response. Role and mechanisms through p53 promotes cell cycle arrest and apoptosis. Meaning of p53 mutations in tumors. - Cell transformation, general concepts for oncogenesis. Oncogenes and Proto-Oncogenes, fefinition and function. Mechanisms of activation of oncogenes (gain of function). Some examples of oncogenes (tyrosine kinases receptors, cytoplasmic kinase tyrosine, src, bcr-abl, RAS). Cancer suppressors (loss of function); loss of heterozygosity. Definition of caretaker and gatekeeper. Rb; cell cycle inhibitors; p53. - The role of apoptosis in cellular homeostasis. - Extrinsic way for apoptosis activation: pro-apoptotic receptors, death domain (DD) and death effector domain (DED) and the transduction of the apoptotic signal up to the activation of caspases regulators (caspase 8). - Caspase activation mechanism (caspase 3) and the role of their substrates in determining apoptosis (shape change, fragmentation of DNA, phosphoididylserine) and recognition of cells with phagocytic activity. - Intrinsic way for apoptosis activation: central role of regulation of permeability of external mitochondrial membrane in determining the release of cytochrome C, Smac / Diablo and other proteins which regulate apoptosis. Activation of caspases 9 by cytochrome c. Role for the IAP proteins (caspase inhibitors) in apoptosis regulation. - Different regulatory mechanisms for the 3 major caspase families (caspases 8, 9 and 3) - External mitochondrial membrane pore formation: pro-apoptotic proteins Bcl2, proteins Bcl2 and anti-apoptotic one. Functional Meaning of Domains BH1, BH2e BH3. - Necrosis: distinctive features from apoptosis Genetic Manipulations in the Mouse - Standard transgenesis, gene targeting, Cre-Lox systems (constituent vs inducible systems). Examples of mice transgenic or knock-out genes for the study of pathologies (Alzheimer's disease, p53 - / -)
Expected learning objectivesThe student must know the molecular and cellular mechanisms regulating replication, cell growth and the gene expression program in eukaryotic organisms with parallels in prokaryotic organisms.
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CourseRecombinant technology lab
Course IDBT023
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersSANTORO Claudio Ventura, CORAZZARI Marco
CFU5
Teaching duration (hours)16
Individual study time 73
SSDBIO/13 - Experimental biology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CourseRecombinant technology lab
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderSANTORO Claudio Ventura
TeachersSANTORO Claudio Ventura
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageitalian
AbstractPrinciples and some practical aspects of basic recombinant technologies
Reference textsJeremy Dale, et al. Dai Geni ai Genomi ed EDISES III edition James D. Watson et al. DNA ricombinante Ed. Zanichelli II edition
Teaching targetsTo provide principles and operational practice of basic techniques used to express recombinant proteins.
Prerequisitesknowledge of the molecular mechanisms that control cell fate and function
Didattics Methodslecture and lab work
Other informationsavailable on DIR
Grading ruleswritten test with multiple choice or open questions
Full argumentsPCR. Vectors: for cloning and expression in bacteria or eukaryotic cells. Site directed mutagenesis. GMO and respective technologies. Genome editing. RNAi. Principle of proteomic analyses.
Expected learning objectivesTo reach the minimum level of sufficiency, the student must: - Know basic mechanisms involved in gene expression regulation; - know how to draw an expression vector; - know how to identify and find the data and information necessary for the expression of a recombinant protein; - be able to express themselves, both in oral and written form; - be able to read, understand and comment an experimental protocol; To achieve an advanced level, the student must: - know how to integrate basic knowledge to draw a vector; - to know how to intervene on one or more cellular mechanisms in order to modify specific cellular functions.
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CourseRecombinant technology lab
Academic Year2017/2018
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCORAZZARI Marco
TeachersCORAZZARI Marco
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
Abstracttheoretical principles and some practical aspects of the main recombinant DNA techniques
Reference texts- Jeremy Dale, et al. "Dai Geni ai Genomi" - EDISES (terza edizione) - James D. Watson et al. "DNA Ricombinante" - Zanichelli (seconda edizione) - Terry A. Brown "Biotecnologie Molecolari" - Zanichelli (seconde edizione)
Teaching targetsProvide the theoretical and practical bases of the methods and technologies used in the study and analysis of gene expression, with particular emphasis on recombinant DNA techniques used to clone, express and analyze gene products of biomedical interest and applications. Importantly, the frequency of a dedicated laboratory is mandatory.
PrerequisitesThe student must know both cellular biology and basic genetics, and in particular the molecular and cellular mechanisms regulating replication, cell growth and gene expression programs in both prokaryotic and eukaryotic organisms.
Didattics Methodsclassroom and laboratory lessons
Other informationsavailable on DIR website
Grading ruleswritten test (multiple-choice questions)
Full argumentsCloning. Gene manipulation strategies. Key features of cloning vectors. Expression of recombinant proteins. Strategies to generate and maintain genetically modified organisms. Technologies used in genetic diagnosis. Cloning and analysis of genotypes. PCR: Principles and Uses. Targeted mutagenicity. Expression of recombinant proteins in E. coli. Methods of gene transformation. Expression of recombinant proteins in eukaryotic cells. Expression of transgenes in animal models. DNA arrays, Gene-chips. PTT, SNP. Proteomics. Genetic strategies to manipulate the phenotype of organisms of socio-economic interest. Animal & Plant Pharming.
Expected learning objectivesThe student will become familiar with the main methods and technologies used to study and analyze gene expression, together with the main techniques used to clone, express and analyze gene products of biomedical interest and application. Acquired familiarity with instrumentation and working methods of a research / analysis laboratory.
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CourseBiotechnological applications in the clinical laboratory
Course IDMS0371
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderROLLA Roberta
TeachersCRISA' Elena, ROLLA Roberta
CFU10
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year3
PeriodSecondo Semestre
SiteNOVARA
Grading typeFinal grade
Modules
Course ID Course SSD Teachers
BT011 Hematology MED/15 - Blood diseases ROSSI Davide, CRISA' Elena
BM047 Clinical pathology MED/05 - Clinical pathology ROLLA Roberta
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CourseHematology
Course IDBT011
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderROSSI Davide
TeachersCRISA' Elena
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/15 - Blood diseases
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Teaching languageItaliano
Reference texts• R. Hoffman, E.J. Benz, Jr., S.J. Shattil, B. Furie, H.J. Cohen, L.E. Silberstein, P. McGlave (editords). HEMATOLOGY. BASIC PRINCIPLES AND PRACTICE. 6th edition. Churchill Livingstone, New York, 2012 Agli studenti verrà fornito al termine delle lezioni il set delle slides presentate a lezione
Teaching targetsSono obiettivi del modulo: 1. Conoscere ed interpretare la diagnostica morfologica delle malattie del sangue 2. Conoscere ed interpretare un esame emocromocitometrico 3. Conoscere ed interpretare i test diagnostici per le anemie ereditarie 5. Conoscere le applicazioni della citogenetica convenzionale e molecolare alla diagnostica e gestione delle malattie del sangue 6. Conoscere le applicazioni dei test molecolari alla diagnostica e gestione delle malattie del sangue 7. Conoscere le applicazioni dei test citofluorimetrici alla diagnostica e gestione delle malattie del sangue 8. Conoscere ed interpretare i test di medicina trasfusionale 9. Conoscere le applicazioni biotecnologiche al trapianto di cellule staminali emopoietiche 10. Conoscere le applicazioni alle malattie del sangue della genomica (gene expression profille, SNP array, GWAS, whole genome/exome sequencing)
PrerequisitesLo studente deve essere in possesso dei fondamenti della biologia cellulare, della immunologia, della citogenetica, della biologia molecolare, della istologia e citologia
Didattics MethodsAllestimento, colorazione e valutazione al microscopio ottico di uno striscio di sangue periferico Allestimento e valutazione di preparato mediante FISH Interpretazione di una sequenza Sanger per la identificazione di mutazioni Interpretazione di una PCR quantitativa per il trascritto di fusione BCR/ABL Presentazioni in formato MS-Power Point, siti internet didattici
Other informationsGli studenti hanno la possibilità di eseguire un internato presso il Laboratorio di Ematologia. Durante la frequenza presso il Laboratorio di Ematologia, lo studente sarà affiancato ad uno dei Biologi avviato ad alcune tecniche di diagnostica in Ematologia, con particolare riguardo alla diagnostica citomorfologica, alla diagnostica citogenetica ed alla diagnostica molecolare applicata alla oncoematologia.
Grading rulesLa verifica dell’apprendimento avverrà tramite prova scritta con quiz a scelta multipla.
Full arguments• Morfologia delle cellule del sangue 1. Tecnica dello striscio di sangue periferico 2. Colorazione May Grunwald/Giemsa 3. Morfologia filologica e patologica dell'eritrocita 4. Morfologia fisiologica e patologica del leucocita 5. Morfologia fisiologica e patologica delle piastrine 6. Morfologia fisiologica e patologica dei precursori emopoietici • Applicazioni citogenetiche alle malattie del sangue 1. Principi del cariotipo convenzionale 2. Principi del cariotipo molecolare FISH 3. Le principali anomalie citogenetiche associate alle malattie del sangue • La biologia molecolare delle malattie del sangue 1. Analisi del riarrangiamento delle immunoglobuline nella diagnostica delle malattie del sangue 2. Analisi del riarrangiamento del recettore T nella diagnostica delle malattie del sangue 3. Analisi mutazionale nella diagnostica delle malattie del sangue ereditarie ed acquisite 4. Ricerca dei trascritti di fusione nella diagnostica delle malattie del sangue 5. Analisi della malattia minima residua mediante approcci di biologia molecolare e RT-PCR 6. Analisi dell'attecchimento midollare dopo trapianto di cellule staminali emopoietiche allogeniche tramite Short Tandem Repeats • La diagnostica citofluorimetrica applicata alle malattie del sangue 1. Diagnostica citofluorimetrica delle leucemie 2. Analisi della malattia minima residua mediante citofluorimetria • Test di medicina trasfusionale 1. I gruppi ematici 2. I test di compatibilità • Trapianto di cellule staminali emopoietiche 1. Il sistema HLA 2. Raccolta e preparazione delle cellule staminali per il trapianto 3. Manipolazione del graft per il trapianto 4. Monitoraggio dell'attecchimento e della ricostituzione immunologica • Genomica delle malattie del sangue 1. Gene expression profile 2. Genomw wide association studies 3. SNP array 4. Whole genome and whole exome sequencing
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CourseClinical pathology
Course IDBM047
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderROLLA Roberta
TeachersROLLA Roberta
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/05 - Clinical pathology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Teaching languageItalian
AbstractThe course describes the biotechnology used in Clinical Pathology.
Reference textsTeaching Material provided by the lecturer. Lecturer's Monographs.
Teaching targetsProvide a specific knowledge of biotechnology used in Clinical Pathology.
PrerequisitesGood knowledge of Chemistry, Biochemistry, Molecular Biology and Genetics.
Didattics MethodsPowePoint presentation. Teaching Network on DIR Website. Frontal lessons. Laboratory exercises.
Other informationsLaboratory exercises at Clinical Chemistry Unit, "Maggiore della Carità" Hospital of Novara, during which will be deepened the topics previously discussed in class, for a total of 12 hours/student.
Grading rulesMultiple choice test.
Full arguments40 hours of lectures, in which are described the topics listed below. A. Molecular Diagnostics in Clinical Pathology, in particular the following molecular biology techniques are described: • RealTime PCR (FRET probes) • Hybridization with ASO (Allele-Specific Oligonucleotide) • Microarray • Pyrosequencing • Mass spectrometry for nucleic acids analysis. Then are evaluated the molecular biology tests used in the diagnosis of the following diseases: • Cystic Fibrosis • Y chromosome microdeletion • Hereditary thrombophilia • Hemochromatosis In particular, the pathophysiological, biochemical and molecular bases of these pathologies are described. B. Description of Biotechnology used in Clinical Pathology for the study of hemostasis and coagulation. In particular: • biotechnologies used in the evaluation of coagulation: thrombotic risk and bleeding risk (screening tests and specialized tests). • biotechnologies used in the evaluation of platelet function (reticulated platelets, PFA, Multiplate, aggregometry). C. Description of Biotechnology used in Clinical Pathology for the study of blood diseases (red line and white line) and for the evaluation of body fluids (cerebrospinal fluid, ascites, pleural fluid, synovial fluid, cardiac liquid). In particular: • blood count • biochemical parameters for the evaluation of anemia • flow cytometry for leukaemias phenotyping • ion exchange chromatography for the evaluation of hemoglobinopathies and thalassemia. In particular are described the pathophysiological, biochemical and molecular bases of α and β thalassemia. D. Electrolyte, and Acid Base Balance E. Electrophoresis of human plasma proteins F. Biochemical markers of myocardial injury G. Renin-angiotensin-aldosterone system H. The clinical use of thyroid function tests I. Lipoprotein metabolism J. Pathophysiology of atherosclerosis
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CourseClinical Biochemistry and Clinical Molecular Biology
Course IDMS0450
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCAPELLO Daniela
TeachersCAPELLO Daniela
CFU5
Teaching duration (hours)40
Individual study time 85
SSDBIO/12 - Clinical biochemistry and molecular biology
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year3
PeriodSecondo Semestre
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal grade
Teaching languageItalian
AbstractBiomarcatori proteomici, metabolomici e genomici: definizione e ambito di utilizzoFattori dipendenti dalle fasi preanalitica, analitica e post-analitica che influenzano il risultato di laboratorio. Significato e limiti delle indagini di laboratorio: valutazione della qualità e del valore informativo del risultato di laboratorio.Biomarcatori genomici e test molecolari: applicazioni cliniche della biologia molecolare. Le principali tecniche di biologia molecolare applicate alla diagnostica: principi chimico-fisici e ambiti di applicazioneBiomarcatori proteici e biomarcatori enzimaticiBiomarcatori metabolici e disordini metabolici ereditari - Modificazioni metaboliche nelle cellule tumorali - Alterazioni nelle vie di segnalazione nei tumori -Alterazioni nelle vie di segnalazione nelle malattie metaboliche
Reference textsAntonozzi-Gulletta Medicina di Laboratorio Logica e Patologia Clinica, Piccin
Teaching targetsCapire come le tecniche biochimiche e di biologia molecolare vengono utilizzate in ambito diagnosticoComprendere l’ambito di utilizzo dei biomarcatori enzimatici di interesse diagnostico e della metabolomica applicata alla diagnosi dei disordini metaboliciAcquisire le conoscenze di base sull'utilizzo di metodologie "genome wide" utilizzate nella ricerca traslazionale, applicate al concetto di “medicina personalizzata”, con particolare attenzione all'ambito oncologicoEssere in grado di utilizzare le conoscenze teoriche e applicative delle diverse metodologie di diagnostica biochimica e molecolare come strumenti per ideare, ottimizzare e applicare specifiche procedure diagnostiche in risposta ai diversi quesiti cliniciAcquisire una visione d’insieme sulla regolazione dei processi metabolici nei diversi organi e tessuti e comprendere come singole alterazioni a livello delle vie di segnalazione intracellulare possano compromettere l’omeostasi non solo della cellula o del tessuto, ma dell’intero organismo
PrerequisitesConoscenze di base sulle vie metaboliche, traduzione del segnale e regolazione dell'espressione genica
Didattics MethodsLezioni frontali mediante la proiezione di diapositive. Esecuzione in aula di problemi, quiz ed esposizione di casi per presentare precise problematiche di diagnostica molecolare e guidare lo studente alla loro risoluzione. Possibilità di eseguire a casa problemi, quiz e risoluzione di casi tramite la piattaforma moodle. Per la preparazione dell'esame gli studenti potranno utilizzare il materiale fornito dal docente (copia pdf delle diapositive proiettate a lezione, di dispense preparate dal docente e di alcuni articoli tratti da riviste scientifiche che approfondiscono gli argomenti trattati durante il corso) e sui libri di testo consigliati
Other informationsCopia pdf delle diapositive proiettate, il materiale di approfondimento e tutte le informazioni riguardanti il corso e le modalità di esame saranno rese disponibili sul DIR (https://www.dir.uniupo.it/ )
Grading rulesProva scritta strutturata in domande con risposte a scelta multipla, domande con scelta vero e falso e beve motivazione della scelta, e domande a risposta aperta.
Full arguments1_Introduzione I biomarcatori - Biomarcatori proteomici, metabolomici e genomici. - Finalità e significato dei test diagnostici: markers molecolari Le fasi preanalitica, analitica e post-analitica delle indagini di laboratorio - Variabilità del materiale biologico. - Significato e limiti delle indagini di laboratorio. - Definizione dei valori di riferimento e dei valori soglia. Sensibilità e specificità diagnostica; valore predittivo di un test diagnostico 2_ Biomarcatori genomici: applicazioni cliniche della biologia molecolare La biologia molecolare in ambito clinico - Alterazioni genetiche indagate in diagnostica molecolare. Potenzialità e limiti dei test molecolari - PCR qualitativa e quantitativa real-time: principi ed esempi di applicazione per la determinazione di lesioni genetiche - Dal sequenziamento mediante tecnica Sanger al sequenziamento di IV generazione - Sequenziamento di mRNA. - Identificazione delle variazioni strutturali del DNA. Principi ed applicazioni in diagnostica oncologica e delle malattie congenite 3_Biochimica Clinica degli organi e dei sistemi Biomarcatori proteici - Le proteine del plasma: ruolo e variazioni quantitative - Le proteine reattive della fase acuta: classificazione e cenni sulla loro regolazione Biomarcatori enzimatici - Criteri di scelta per i biomarcatori enzimatici nel laboratorio clinico. Metodi di determinazione delle attività enzimatiche - Principali enzimi utilizzati nel laboratorio clinico: basi biochimiche della funzione in condizioni di normalità e razionale biologico per il loro utilizzo come marcatori di malattia. Quadri enzimatici d’organo e condizioni morbose Meccanismi di detossificazione a livello epatico - Reazioni di fase I: la superfamiglia delle citocromo P450 monossigenasi - Reazioni di fase II: reazioni di coniugazione - Metabolismo epatico dell’etanolo Alterazioni congenite del metabolismo - Indagini del metaboloma, del proteoma ed i dosaggi enzimatici: ambiti di applicazione e limiti. - Definizione di screening diagnostico e sue applicazioni. - Esempi di alterazioni congenite del metabolismo indagate o potenzialmente indagabili mediante screening neonatale 4_Biosegnalazione nello stato di salute e di malattia Le vie di trasduzione del segnale. - Fattori di crescita, ormoni e citochine. - Recettori di membrana con attività enzimatica e non. I recettori associati a proteine G eterotrimeriche - Le principali vie di trasduzione del segnale: MAP-kinasi, JAK/STAT; PI3K/AKT/mTOR. Small G-proteins; le adesioni focali Regolazione Ormonale: asse ipotalamico-ipofisario e suoi bersagli periferici - Ormoni surrenalici corticali - Generalità su ormoni gonadotropi - Ormoni sessuali - Ormoni dell'ipofisi media - Ormoni dell'ipofisi posteriore - Ormoni che regolano il metabolismo del calcio e del fosfato Regolazione del metabolismo energetico e sue disfunzioni nella sindrome metabolica - Controllo del metabolismo degli amminoacidi. Controllo trascrizionale del metabolismo energetico. Basi molecolari della sindrome metabolica. Alterazioni nelle vie di segnalazione dell’insulina e nell’omeostasi del metabolismo glicidico e lipidico Metabolismo del ferro - Ciclo del ferro e sua distribuzione nell’organismo. Principali enzimi che utilizzano il ferro: struttura e funzione - L’omeostasi del Fe. Assorbimento, trasporto e deposito del. Meccanismo d’azione dell’epcidina e sua regolazione - Ruolo dei fattori HIFs nell’omeostasi del Fe. Il sistema di regolazione dei fattori HIFs: le di ossigenasi -chetoglutarato dipendenti - Disordini dell’omeostasi del Fe: le emocromatosi Modificazioni metaboliche nelle cellule tumorali - Meccanismi generali di regolazione della glicolisi in presenza o in carenza di ossigeno, l’effetto Pasteur, l’effetto Warburg nelle cellule tumorali. La fermentazione lattica. La via glicolitica ed il ciclo di Krebs per l’ anabolismo. La glutamminolisi - Geni e metabolismo: MYC e PI3K/AKT PFK1 e PFK2. PFK2 nei tumori. Le esochinasi nelle cellule tumorali. Le piruvato chinasi: ruolo di PKM2 nel metabolismo glicolitico e nella regolazione della trascrizione genica. Mutazioni dei geni IDH1, IDH2, FH e SDH nei tumori: ruolo diagnostico e significato funzionale. Ipotesi della deregolazione degli enzimi demetilasici nella patogenesi delle neoplasie con mutazioni nei geni IDH1 e IDH2 Diagnostica molecolare in oncologia - Alterazioni delle vie di EGFR, MET/HGF, PDGF/PDGFR, MAPK, PI3K/AKT/MTOR: utilizzo in ambito diagnostico e terapeutico
Expected learning objectivesAl termine del corso lo studente sarà in possesso delle conoscenze necessarie per comprendere: 1) i meccanismi base di regolazione metabolica di organi ed apparati e la loro omeostasi reciproca. 2) le principali alterazioni nelle vie di segnalazione caratteristiche delle malattie oncologiche e dei disordini metabolici3) le applicazioni dei markers biochimici nella diagnostica di laboratorio4) i principi delle principali tecniche utilizzate in diagnostica molecolare e dei loro ambiti di applicazione
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CourseDissertation Thesis
Course IDBT063
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
CFU10
Individual study time 250
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryE - Prova finale e lingua straniera
Year3
PeriodAnnuale
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal judgment
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CourseElective Didactic Activities
Course IDMC117
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
CFU12
Individual study time 300
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryD - A scelta dallo studente
Year3
PeriodAnnuale
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal grade
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CourseEpidemiology
Course IDMS0448
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBARONE ADESI Francesco
TeachersFAGGIANO Fabrizio, BARONE ADESI Francesco
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/42 - Hygiene and public health
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodPrimo Semestre
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal grade
Teaching languageItalian
AbstractFundamentals of Philosophy of Science applied to biomedical research;Epidemiological methods in biomedical research; the Syntesis of knowledge in biomedical research and its transfer into medical practice; the process of drug discovery and development; the communication of the results in biomedical research; Ethical Issues in biomedical research
Reference textsFaggiano, Donato, Barbone. Manuale di Epidemiologia per la sanità pubblica. Centro Scientifico Editore. Rothman. Epidemiology: An Introduction. Oxford press.
Teaching targetsKNOWLEDGE AND UNDERSTANDING To know the most basic concepts of philosophy of science applied to biomedical research To know the most important sources of epidemiological data. To understand the elements in the design and conduct of the most important types of epidemiological studies. To know the most important sources of bias in epidemiological studies. to understand the criteria for characterizing the causality of associations in Medicine. to know the key features of a systematic review of the scientific literature to know the key features of the process of drug discovery and development. to understand the key features of Evidence-Based Medicine. To know the basic features of the communication of results in biomedicalresearch To know the most important ethical aspects of biomedical research. APPLYING KNOWLEDGE AND UNDERSTANDING to be able to calculate measures of frequency and association to be able to interpret the results of an epidemiological study to be able to interpret the results of a meta-analysis
PrerequisitesNone
Didattics MethodsLectures
Other informationsNone
Grading rulesWritten examination, during which it will be evaluated the ability of the student of applying the knowledge acquired during the course. The written exam is constituted of 13-15 questions of the following types: multiple choice questions; calculation of epidemiological measures introduced in the course. The level of difficulty of the exercises corresponds to those explained in class or presented in the handbooks reported in DIR. The pass level is 18/30. During the exam the student is not allowed to use any type of teaching material.
Full argumentsFundamentals of Philosophy of Science applied to biomedical research. The scientific method. The concepts of model, theory and hypothesis in science. Inductive and deductive reasoning. Epidemiological methods in biomedical research. The concept of Health and its evolution over time. Definition of Hygiene and Public Health. Introduction to medical statistics (sources of data; fonti; elaboration and presentation of epidemiological data; health indicators: incidence, mortality, prevalence, survival; measures of frequency and association). Main Epidemiological studies: clinical trials, cohort studies, case-control studies, cross-sectional studies, descriptive studies. Sources of uncertainty in epidemiology: the role of chance, bias and counfounding. Effect modification. Use of test of hypothesis and confidence intervals to interpret the results of an epidemiological study. The concept of statistical power applied to the interpretation of the results of an epidemiological study. Causal inferenc ein epidemiology. the Syntesis of knowledge in biomedical research and its transfer into medical practice. Systematic reviews, meta-analyses, guide-lines, Evidence-Based Medicine. The process of drug discovery and development. Epidemiological studies used in the different stages of the development of a drug. Introduction to pharmacoepidemiology and its applications to to the study of Adverse Drug Events. the communication of the results in biomedical research. Structure of a scientific report. The process of submission and reviewing of a scientific report. The scientific press. Ethical Issues in biomedical research. The conflict of interest. Disease mongering.
Expected learning objectivesKNOWLEDGE AND UNDERSTANDING To know the most important sources of epidemiological data. To understand the elements in the design and conduct of the most important types of epidemiological studies. To know the most important sources of bias in epidemiological studies. to understand the criteria for characterizing the causality of associations in Medicine. to know the key features of a systematic review of the scientific literature to know the most important determinants of health in Italy and worldwide to know the differences between primary, secondary and tertiary prevention. to know the key features of the process of drug discovery and development. to understand the key features of Evidence-Based Medicine. to know the key features of National Health Systems. APPLYING KNOWLEDGE AND UNDERSTANDING to be able to calculate measures of frequency and association to be able to calculate sensitivity, specificity, and predictive values of a clinical test to able to interpret the results of a meta-analysis
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CourseFurther Learning Activities
Course IDMS0120
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
CFU2
Individual study time 100
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryF - Stage e altre attività formative
Year3
PeriodAnnuale
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal judgment
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CourseGeneral Pathology
Course IDMS0447
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCARINI Rita
TeachersCARINI Rita, DIANZANI Irma
CFU6
Teaching duration (hours)48
Individual study time 102
SSDMED/04 - Experimental medicine and pathophysiology
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodPrimo Semestre
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal grade
Teaching languageItalian
Abstract1) INTRODUCTION TO GENERAL PATHOLOGY 2) THE GENETIC BASE OF THE DISEASES 3) PHYSICAL AGENTS AS CAUSE OF DISEASES 4) CHEMICAL AGENTS AS CAUSE OF DISEASES 5) BIOLOGICAL AGENTS AS CAUSE OF DISEASES 6) TISSUE MODIFICATIONS IN RESPONSE TO PATHOLOGICAL CHRONIC AND ACUTE STIMULA 7) INFLAMMATORY PROCESS 8) REPAIR PROCESS 9) NEOPLASTIC GROWTH
Reference textsPatologia e Fisiopatologia Generale di Pontieri, Russo, Frati (Ed. Piccin) Istituzioni di Patologia Generale. M.U. Dianzani (ed. UTET) La Professione del Medico vol.3, (ed UTET)
Teaching targetsThe specific aim of the PATOLOGIA GENERALE course is to understand the root causes (etiology) and mechanisms (pathogenesis) that are responsible for altering the state of health. To this end, the molecular, cellular and super-cellular effects of endogenous pathogens (genetic and tumor diseases) and exogenous pathogens (physical, chemical and biological causes of disease) will be examined. Cell and tissue levels will then be investigated for the adverse consequences of chronic and acute pacing with pathogens (degeneration, reversible and irreversible cell damage, cell death for necrosis or apoptosis). The local and systemic reaction to tissue damage will be described by thorough examination of the inflammatory process and consequent tissue repair. The course of GENERAL PATHOLOGY conducted for the Student of first Degree in BIOTECHNOLOGIES within the Extended Program reported below, will examine in particular the molecular mechanisms of the various pathological conditions examined with specific references to the implications for future Medical Research activities.
PrerequisitesThe Course needs the previous knowledge of fundamental elements of biochemistry, biology, genetics and physiology acquired in previous or parallel courses, which will provide the basis for the learning of the effects of exogenous and endogenous disease agents on the cellular and tissue levels ( Cell damage and neoplastic transformation) and non-specific (inflammatory) and specific (immune) defense responses.
Didattics MethodsLectures based on a continuous student interaction that will allow immediate verification of understanding of the topics discussed. Strongly recommended is the acquisition of notes of all the lessons directly taken by the student such notes will be essential for the preparation of the exam. All slides of the course will be available since from the first lesson.
Grading rulesWe will evaluate the students with a written examination, with questions and multiple choice answers. The questions will be distributed to cover the all program. The questions will be prepared to both verify the knowledge and the understanding of single aspects of the program.
Full argumentsINTRODUCTION TO GENERAL PATHOLOGY; The state of health, the concept of etiology, the concept of pathogenesis, the state of illness. GENETIC DISEASE BASES General on genetic mutations and their causes. Examples of genetic diseases: sickle cell anemia and alterations in transport or metabolism of amino acids: Hartnup disease, phenylalaninemia, albinism. PHYSICAL AGENTS AS A CAUSE OF DISEASE: generality about mechanical, thermal, electric power transfer pathologies. Effects of ionizing radiation (molecular, cellular and super-cellular) and the effects of exciting radiations. CHEMICAL AGENTS AS A DISEASE: absorption and elimination routes; biotransformation reactions: phase 1 (dependent on cytochrome P450) and phase 2; adverse effects of reactive metabolites produced during biotransformation), toxicity by chemical agents . The damage caused by free radicals: production mechanisms, protective factors and cell damage. BIOLOGICAL AGENTS AS A CAUSE OF DISEASE: Generalities on the mechanisms of damage and defense against viral and bacterial infections. TISSUE CHANGES IN RESPONSE TO CHRONIC AND ACUTE PATHOLOGICAL STIMULES: hypertrophy, hyperplasia, hypotrophy, atrophy, metaplasia; Reversible cell damage. Irreversible cell damage: cell death for necrosis and apoptosis. INFLAMMATORY PROCESS: Inflammatory cells and their functions (monocytes, macrophages, neutrophil granulocytes, basophils, mast cells, endothelial cells, platelets) soluble inflammatory mediators (histamine, serotonin, arachidonic acid metabolites, plasma proteases, cytokines: interleukin 1.6 [IL-1 and IL-6], tumor necrosis factor alpha [TNFalfa], transformant-beta growth factor [TGFbeta], bacterial metabolites). Alteration of the tone and vascular permeability during inflammation (exudate formation). Activity of phagocytic cells in inflammation (adhesion, diaphytesis, chemotaxis, phagocytosis, bacterial killing mechanisms); istoflogosi. Systemic effects of inflammation. REPAIR PROCESS: regeneration (tissue proliferative potential, growth factors: epidermal growth factor [EGF], transformant [TGFbeta], similar insulin [IGF], platelet derivation [PDGF], fibroblasts [FGF], interactions With matrix and other cells) substitution with connective tissue and angiogenesis. Wound repair NEOPLASTIC GROWTH: General characteristics of the neoplasms (information on incidence, mortality and survival, tumor classification and nomenclature); Hyperplasia, neoplasia, anaplasia and dysplasia. Viral and cellular oncogenes, oncosoppressor genes. Neoplastic phenotype: phenotypic heterogeneity and genomic instability. Initiation, promotion of the neoplastic process (carcinogenic stages or cancerogenesis as a continuous process). The progression of the neoplastic process (neoplastic latency, neoplastic invasion, metastases).
Expected learning objectivesThe student is expected to obtain the knowledge and the understanding of the molecular base of the loss of health (disease status). Specifically the student is expected to know the causes and the pathogenetic mechanisms of fundamental diseases induced by physical, chemical, biological agents and by genetic alterations; the mechanisms of cell damage, inflammation, reparation and tumor transformation.
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CoursePharmaceutical Chemistry
Course IDMS0449
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPIRALI Tracey
TeachersSORBA Giovanni, PIRALI Tracey, MASSAROTTI Alberto
CFU5
Teaching duration (hours)40
Individual study time 85
SSDCHIM/08 - Pharmaceutical chemistry
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodSecondo Semestre
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal grade
Teaching languageitalian
AbstractMedicinal Chemistry definition. Pharmaceutical Phase Routes of administration. Pharmacokinetic Phase Absorption. Distribution. Elimination. Metabolism. Excretion. Pharmacodynamic Phase Intermolecular interactions. Role of steoreochemistry in the interaction with the binding site. Receptors as drug targets. Enzymes as drug targets. Miscellaneous drug targets. Drug discovey Hit compound and lead compound. Finding a lead. How to recognize drug-like molecules. Molecular modelling. Drug design The optimization of target interactions. The optimization of the access to the target. Drug development Preclinical and clinical trials. Examples of classes of drugs. Biotech drugs. Exercises.
Reference textsChimica Farmaceutica Graham L. Patrick Foye’s Principi di Chimica Farmaceutica Thomas L. Lemke, David A. Williams Medicinal Chemistry: The Modern Drug Discovery Process, E. Stevens Chimica Farmaceutica A. Gasco
Teaching targetsThe aim of the course is to introduce the student to the main principles of medicinal chemistry. The specific objectives of the course, according to the Dublino descriptors, are the following: 1. Knowledge and understanding. At the end of the course the student will know and understand the basic concepts of medicinal chemistry, in particular the route followed by the drug in the organism, from its administration to its excretion (pharmaceutical, pharmacokinetic, pharmacodynamic phases). Moreover, the itinerary of the drug in the pharmaceutical industry, from drug discovery to drug development through drug design, will be addressed. 2. The student will apply the acquired knowledge and understanding in the recognition of the chemical structures, in the discussion of the chemical and chemico-physical properties, of the mechanism of action, of the structure-activity relationship, of the metabolic fate and of the plausible routes for the synthesis of drugs. 3. The student will acquire the ability to identify and use data to formulate responses to well-defined concrete and abstract problems regarding the structure-activity relationship, the metabolism, the related toxicity, the synthesis, the drug-drug interaction for specific cases of drugs. Moreover, the student will be provided with all the tools necessary for the critical analysis of texts and papers in the medicinal chemistry literature. 4. The student will learn to communicate about his/her understanding, to describe, even in an original way, a topic described during lessons, to adequately answer to questions, critiques and suggestions. 5. The student will have the learning skills to handle in a dinamic way his/her knowledge in medicinal chemistry with some autonomy.
PrerequisitesIn order to give the exam in Medicinal Chemistry I it is necessary that the student has passed the exam in Organic Chemistry
Didattics MethodsThe course is given with the support of power point slides, which are furnished to the students at the beginning of the lessons. The last lessons consist in practice exercises with the aim of preparing the student to the exam.
Grading rulesThe exam consists in a computer-based written test displaying questions (multiple choice, brief answer, true/false, missing word, etc). The exam aims at verifying both the knowledge on the contents of the course and the ability to apply this knowledge to specific cases of drugs used in therapy. The student has to demonstrate to be able to o identify the functional groups, the acid, basic and stereogenic centers, the plausible reactions of metabolism, the intermolecular interactions, etc. Through these tests, it will be verified that the student has achieved the objectives of knowledge and understanding of the contents of the course, the ability to communicate these concepts in a clear and correct manner and the competence to apply the acquired knowledge and skills to specific concerns regarding the medicinal chemistry of the different classes of drugs.
Full argumentsMedicinal Chemistry definition. Pharmaceutical Phase Routes of administration. Pharmacokinetic Phase Absorption. Mechanisms of absorption. Passive diffusion. Partition coefficient. Fick law. Henderson-Hasselbach equation. Ion-pair absorption. Carrier mediated transport. Vescicolar transport. Convective trasport. The absorption in the oral administration: first-pass metabolism. Distribution. Plasma protein binding. Accumulation. Blood-brain barrier. Placental barrier. Elimination. Renal excretion. Phase I and phase II metabolism. Soft e hard drugs. Pharmacokinetic concepts. Plasma concentration-time curve. Clearance. Half-life. Bioavailability. Apparent volume of distribution. Factors that influence pharmacokinetics. Pharmacodynamic Phase Intermolecular interactions. Covalent bond. Ionic bond. Dipole-dipole interaction. Ion-dipole interaction. Hydrogen bond. Halogen bond. Charge transfer. Van der Waals interactions. Hydrophobic interactions. Pi greco-pi greco interactions. Role of steoreochemistry in the interaction with the binding site. Three point contact model. Pfeiffer rule. Eutomer, distomer, eudismic ratio. Chiral switch. Methods for the chiral switch. Receptors as drug targets: theories, strategies to design agonists, antagonists, allosteric modulators, inverse agonists. Enzymes as drug targets: competitive reversible, non competitive reversible, irreversible inhibitors, transition-state analogues, suicide substrates. Miscellaneous drug targets: transport proteins as drug targets, structural proteins as drug targets, protein-protein interactions, lipids as drug targets. Hit compound and lead compound. Choosing a disease, a drug target, a bioassay. High-throughput screening, NMR screening, virtual screening. Parallel and combinatorial synthesis. Finding a lead: natural products, serendipity, natural ligand modifications, me-too drugs, SOSA approach, screening, de novo drug design, fragment-based drug design. How to recognize drug-like molecules. Lipinski and Veber rules. The use of computer in drug discovery. Manipolation of chemical structures, conformational analysis, minimization, parametrization. The use of chemical and biological data banks, chemical similarity and X-ray structures. Ligand- and Structure-Based Drug Design. Virtual screening. De novo drug design. 3D-QSAR. Pharmacophore- Molecular docking, applications and drawbacks. Proteins. Resolution in 3D protein structures. Examples of protein purification. X ray cristallography. NMR spectroscopy. Co-cristallization. Quality of experimental data: resolution and B-factor. Protein Data Bank. On-line search of PDB. The structure of a .pdb. PyMol, main functions of the program. Basic procedure to visualize a .pdb in the PyMol software. Drug design The optimization of target interactions: structure-activity relationships, isosteres and bioisosteres, classic and non classic bioisosteres, pharmacophore identification, drug design strategies (extension, chain extension/contraction, homology, vinilogy, benzology, ring expansion/contraction, ring variations, ring fusion, structure simplification, structure rigidification, conformational blockers, twin drugs and hybrids). The optimization of the access to the target: how to optimize the hydrophobic and hydrophilic properties, how to improve the chemical and the metabolic stability, prodrugs, mutue prodrugs. Drug development Preclinical and clinical trials. Examples of specific classes of drugs. Biotech drugs. Exercises.
Expected learning objectivesThe student at the end of the course will know the basic principles of medicinal chemistry, both in pharmacodinamics and pharmacokinetics. Furthermore, he/she will be able to apply these principles to a specific bioactive molecule and to evaluate its chemical, metabolic and drug-likeness properties. The student will also know the role of medicinal chemistry in drug discovery and in drug design and the strategies that can be used in order to improve the pharmacodinamic and pharmacokinetic properties of a bioactive molecule.
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CoursePharmacology and Innovation management
Course IDMS0432
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderFRESU Luigia Grazia
TeachersCONICELLA Fabrizio, FRESU Luigia Grazia, SEDDIO Pasquale, JOMMI Claudio
CFU12
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year3
PeriodPrimo Semestre
SiteNOVARA
Grading typeFinal grade
Modules
Course ID Course SSD Teachers
MS0422 Innovation management and technology transfer SECS-P/08 - Management ABRATE Graziano, CONICELLA Fabrizio
BM030 Pharmacology BIO/14 - Pharmacology FRESU Luigia Grazia
M0201 Managerial economics in life sciences SECS-P/07 - Business administration and Management JOMMI Claudio, SEDDIO Pasquale
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CourseInnovation management and technology transfer
Course IDMS0422
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderABRATE Graziano
TeachersCONICELLA Fabrizio
CFU2
Teaching duration (hours)16
Individual study time 68
SSDSECS-P/08 - Management
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year3
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal grade
Teaching languageItalian Documents in english
AbstractMain topics covered: Creativity, scientific results and innovation Project management Patent information tools Technology transfer Business model/business plans Marketing
Reference textsSlides and material shared Suggested books: Innovation and Entrepreneurship John Bessant Joe Tidd June 2011 John Wiley & Sons Inc; ISBN-10: 0470711442 ISBN-13: 978-0470711446 Managing Innovation: Integrating Technological, Market and Organizational Change, Joe Tidd, John Bessant March 2009 ISBN-10: 0470998105 ISBN-13: 978-0470998106 http://www.innovation-portal.info La Gestione Del Trasferimento Tecnologico - Strategie, Modelli E Strumenti Andrea Piccaluga, Massimiliano Granieri, Giuseppe Conti 1 edizione (25 agosto 2011) ISBN-10: 884701901X ISBN-13: 978-8847019010 Ricerca e sviluppo nell'industria biotecnologica e farmaceutica Silvano Fumero Bollati Boringhieri (23 maggio 2003) ISBN-10: 8833957071 ISBN-13: 978-8833957074 Economia delle aziende biotecnologiche Franco Angeli; 1 edizione (11 agosto 2015), Collana: Università-Economia ISBN-10: 8891714054 ISBN-13: 978-8891714053 Business Planning Cinzia Parolini : Pearson Collana: Studio & professione Febbraio 2011 ISBN-10: 8871926293 ISBN-13: 978-8871926292 Commercializing Successful Biomedical Technologies: Basic Principles for the Development of Drugs, Diagnostics and Devices Shreefal S. Mehta Cambridge University Press; Reissue edizione (30 aprile 2011) ISBN-10: 0521205859 ISBN-13: 978-0521205856 Building Biotechnology: Biotechnology Business, Regulations, Patents, Law, Policy and Science Yali Friedman Editore: Logos Press; 4. Auflage. edizione (1 gennaio 2014) ISBN-10: 1934899291 Project Management: dall'idea all'attuazione. Una guida pratica per il successo Marion E. Haynes Editore: Franco Angeli (11 ottobre 2012) ISBN-10: 8856811650 ISBN-13: 978-8856811650 ISBN-13: 978-1934899298
Teaching targetsThe Management of Innovation and Technology Transfer aims at presenting, with a focus on life sciences, the major issues related to the management of research projects and the innovations resulting from these, as well as the pathways for enhancing the results, both through technological transfer paths that start up innovative businesses. Particular attention will be devoted to the analysis of the industrial aspects of these pathways and to the patent and commercial exploitation of the results of scientific research, including business start-ups, as well as project management and innovation management issues
PrerequisitesMC004 English
Didattics MethodsPowerPoint presentation and internet access for database consultation. Eventual exercise on the evaluation of a scientific result and identification of exploitation path.
Other informationsThe course objective is to present the key concepts underlying the exploitation of research results with a specific focus on life sciences. The student will acquire basic knowledge of innovation management and technological transfer processes in order to understand the processes underlying the possible exploitation paths of the scientific results and identify and, in perspective, manage, the core elements. The student will also acquire the basic knowledge in order to analyze an entrepreneurial idea and to identify and approach the key components of a business plan. Finally, the student will acquire the ability to analyze, interpret and plan start-up and technology transfer activities and understand the dynamics of the industry.
Grading rulesTest multiple choices
Full argumentsTopics covered: •    Creativity, scientific results and innovation •    From the idea to the innovation: management of innovative projects and project management tools •   Patent information tools •    Management evaluation and exploitation of scientific results:patents, startups and technology transfer Business model and business plan •    Innovation business development: innovations marketing •    Technology transfer •    Life sciences: industrial structure
Expected learning objectivesThe student will acquire basic knowledge of innovation management and technological transfer processes in order to understand the processes underlying the possible exploitation paths of the scientific results and identify and, in perspective, manage, the core elements. The student will also acquire the basic knowledge in order to analyze an entrepreneurial idea and to identify and approach the key components of a business plan. Finally, the student will acquire the ability to analyze, interpret and plan start-up and technology transfer activities and understand the dynamics of the industry.
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CoursePharmacology
Course IDBM030
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderFRESU Luigia Grazia
TeachersFRESU Luigia Grazia
CFU5
Teaching duration (hours)40
Individual study time 85
SSDBIO/14 - Pharmacology
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Teaching languageitalian
AbstractBasic principles of pharmacocynetic and pharmacodynamic. Drugs of nervous system; drugs of cardiovascular system; anti-inflammatory drugs; immunosuppressive drugs; antineoplastic drugs; insulin and oral hypoglicemic drugs
Reference textsThe most recent publications cited during the lectures, the following books are recommended: 1. HP Rang and MM Dale – Farmacologia, Casa Ed Ambrosiana 2. Goodman & Gilman – Le basi farmacologiche della terapia di J.G.Hardman, L.E. Limbird, A.Goodman Gilman, Curatore edizione italiana: C.Sirtori, G.Folco, G.Franceschini, S.Govoni. 3. Farmacologia generale e molecolare, a cura di F.Clementi, G.Fumagalli, Ed UTET
Teaching targetsIn the context of this Integrated Course, the module of Pharmacology will give to students the basis of pharmacokinetic and pharmacodynamic principles, the general concepts of “conventional small molecules” and biological/biotechnological drugs along with the most important pharmacological differences and their pre-clinical and clinical development. Moreover, the course will provide detailed information about selected drug classes
PrerequisitesStudents are required to have a good preparation in biochemistry, physiology, anatomy and pathology
Didattics MethodsLectures with power point presentation
Grading rulesWritten test
Full arguments-Introduction to pharmacology - Discovery and development of a new drug: pre-clinical and clinical phases - The main principles of drugs, conventional small molecules, biological/biotechnological drugs, equivalent and biosimilar drugs -Pharmacodynamic: receptors and signal transduction; meaning of agonist and antagonist; the dose-response curve; -Pharmacokineti: absorbtion, distribution, metabolism and excretion of drugs; definition of half life, bioavailability, clearance and distribution volume -Antiinflammatory drugs -Immunosuppressive drugs -Anti-neoplastic drugs Anti-coagulant and anti-thrombotic drugs - Anti-hypertensive drugs - Drugs of nervous system: anti-depressives, anti-parkinson, anti-psycotic drugs - Drug abuse: opioids, psicostimulants, cannabinoids
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CourseManagerial economics in life sciences
Course IDM0201
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderJOMMI Claudio
TeachersSEDDIO Pasquale, JOMMI Claudio
CFU5
Teaching duration (hours)40
Individual study time 85
SSDSECS-P/07 - Business administration and Management
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Teaching languageItalian
AbstractIntroduction to management and biotech market Biotech industry Management of biotech inventions Economic evaluation of (red) biotechnologies
Reference textsThere is not a single textbook. Management of biotech inventions Friedman Y (2013), Building Biotechnology: Biotechnology Business, Regulations, Patents, Law, ThinkBiotech LLC, 4th Edition Bio-pharma industry Gianfrate F, Il mercato dei farmaci tra salute e business, Franco Angeli Editore, 2014 Economic evaluation of red biotechnologies Mennini FS, Cicchetti A, Fattore G, Russo P. La Valutazione Economica dei Programmi Sanitari. Il Pensiero Scientifico ed. 2011. Terza Edizione.
Teaching targetsThe course aims at providing the students with competences and tools to manage research projects, to start a new company, to work in the bio-pharma industry
PrerequisitesNone
Didattics MethodsLectures, Class discussions, Class and homework exercises, Groups Works
Other informationsStudents are expected to participate actively, including Groups Works
Grading rulesFinal written test (open questions and exercises) (80% of final grade); discussion of Groups Works (20% of final grade)
Full argumentsIntroduction to management and (red) biotechnology market - Introduction to management and basics of economics - Introduction to the biotech industry and biotech drugs market - Drugs Research and Development - Drugs Market Regulation - Market access, marketing and sales Management of biotech inventions - Patents and Intellectual Property Rights (introductory aspects, procedures, patent databases, economic evaluation of patents) - Technology Transfer: from idea to market Economic evaluation of (red) biotechnologies - Cost-effectiveness analysis - Budget Impact analysis
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CourseStatistical methods for experimental studies
Course IDMS0446
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMAGNANI Corrado
TeachersMAGNANI Corrado
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/01 - Medical statistics
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryA - Base
Year3
PeriodPrimo Semestre
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal grade
Teaching languageItalian
AbstractFirst level course. Statisical methods for data description. Probability. Sampling. Principles of statistical inference. Tests of common use for the analysis of numerical and categorical data.
Reference textsAll the suggested texts are Italian translations of English textbooks. The students can choose the language as they prefer. M.Pagano & K.Gauvreau. Biostatistica (II edizione italiana). ed. Idelson Gnocchi, Napoli 2003. Further readings: - Daniel G Biostatistica. Ed. Edises.. - Armitage & Berry Metodi statistici nella ricerca medica, 3a edizione (ed inglese Blackwell, tradotto e stampato in Italia da McGraw-Hill). The 4th edition is also available in English only. - Siegel & Castellan Statistica non parametrica. McGraw-Hill. – Text on non parametric tests. - Douglas Altman, David Machin, Trevor Bryant, Martin Gardner Statistics with Confidence (2nd ed.) BMJ edition.. - www.publichealth.ac.nz/ Reading of papers on statistical methosds, as those published on British Medical Journal or British Journal of Cancer.
Teaching targetsData description. Probability. Sampling. The student is expected to get the basis for describing data from laboratory activity and for understanding the basic steps of sampling and study design. Understanding of statistical inference. Basic use and interpretation of statistical tests for categorical and numerical data.
PrerequisitesMathematics as in high schools.
Didattics MethodsThe course will include lectures and exercises. For the latter the students will be divided in small groups. Some topics will be left for individual study.
Grading rulesWritten test (60 min approx.) The test includes: -open questions; - exercise solution; - check – list - summary questions on the interpretation of the result of the statistical test. The entire program will be considered in the final test, including the topics left for individual study.
Full arguments1) Presentation General concepts Presentation of the data - Types of data. - Absolute, relative, cumulative frequencies. - Tables of contingency. - Graphical representations of frequency distributions. Chapters of reference: 1 & 2 2) Synthesis and presentation of numerical data Indices of position (mean, median, mode) and of dispersion (standard deviation, coefficient of variation, percentiles). Graphical representations of numerical data. Box plot Geometric mean, logarithmic transformation. Calculation of the average for grouped data. Description of data defined from two variables. - Dot diagrams Chapters of reference: 2 & 3 3) Probability Definition of probability. Calculation of the probability of an event and the combined probability of more events. Conditional probabilities - Application: sensibility, specificity and predictive value of a diagnostic test. Chapters of reference: 6 4)Methods sampling chapter 22 5) Theoretical Distributions of probability Binomial distribution. Calculation of the probability of an event with binomial probability distribution. Normal distribution Standard Normal distribution. Use of the tables of the standard normal distribution. Chapters of reference: 7 5) Sampling and distribution of sample means Definition of population and sample. Reasons for sampling. Relation between population and sample and property of collections of samples. Theorem of the central limit. Applications of the theorem of the central limit. Sample size Chapters of reference: 8 6) The Student t distribution Definition Degrees of freedom. Applications Use of EXCEL statistical functions for the normal distribution and the t distribution. Chapters of reference: 9 Confidence intervals Definition Calculation of the confidence interval for the average, in the cases with known and unknown population variance. Generalization of the calculation of the confidence interval to other statistics. Chapters of reference: 9 7)Test of hypothesis Introduction to the inferential statistics Work Hypothesis and null hypothesis Error of I° and II° type Chapters of reference: 10 8) Comparison between two averages Use of the t-Student Test. Use of the tabulated t distribution and the functions of Excel. - Sample with paired observations. - Independent samples with equal variance. - Confidence interval based on the t distribution. Chapters of reference: 11 9) Analysis of the data in contingency tables Tables of contingency with two lines and two columns (2x2). - Chi-square test. - Association: Odds Ratio and Relative Risk. - Confidence interval of the Odds ratio - Correction of the continuity - Exact test of Fisher - Test of McNemar Extension to tables n x m. Degrees of freedom. Chapters of reference: 15 10) Analysis of the variance - analysis of the variance with one classification criterion Chapters of reference: 11 11) Simple linear regression The regression straight line. The esteem with the least-squares method. Statistical test of regression coefficients. Use of the residuals in order to verify the validity of the assumptions. Confidence intervals for prediction. Reading results of multivariable analysis Chapters of reference: 18 12) Introduction to the design of the study Statistical power Arguments of individual study on the reference text (without lectures) Tests “distribution free” or distribution free General introduction Advantages and disadvantage Comparison with the parametric correspondents tests. Test U di Mann-Whitney Test of Wilcoxon for paired data Chapters of reference: 13 Correlation: Pearson & Spearman (reference: 17)
Expected learning objectivesAims: Basic knowledge for the reading of papers in biotechnology with statistical content. Presentation of data. Interpretation of laboratory results and clinical tests.
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CourseTraining
Course IDMS0146
Academic Year2017/2018
Year of rule2015/2016
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
CFU8
Individual study time 200
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryF - Stage e altre attività formative
Year3
PeriodAnnuale
SiteNOVARA
Frequenza obbligatoriaYes
Grading typeFinal judgment
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