Laurea in Biotecnologie

Academic program

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Year Course ID Course Teachers SSD Curriculum Site CFU
1 MS0729 Fundamentals of mathematics and physics RINALDI Maurizio, PANZIERI Daniele FIS/01, MAT/04 All 12
1 MS0734 Genetics GIORDANO Mara, PERSICHETTI Francesca, SOLURI Angela Maria Felicia, CORRADO Lucia, BARIZZONE Nadia BIO/18, MED/03 All 9
1 MS0731 Histology and anatomy PRAT Maria Giovanna, BOZZO Chiarella, COTELLA Diego, FOLLENZI Antonia, BOCCAFOSCHI Francesca BIO/13, BIO/17, BIO/16 All 15
1 MS0725 Inorganic chemistry BOCCALERI Enrico, GABANO Elisabetta, MINASSI Alberto, CAPRIOGLIO Diego CHIM/03, CHIM/06 All 9
1 MS0733 Organic Chemistry APPENDINO Giovanni Battista, NEGRI Roberto, CAPRIOGLIO Diego CHIM/06 All 6
2 MS0744 Basics of immunology and medical microbiology AZZIMONTI Barbara, DIANZANI Umberto, CHIOCCHETTI Annalisa MED/04, MED/07 All 10
2 MS0737 Biochemistry BALDANZI Gianluca, FERRARIS Davide Maria, CAPELLO Daniela, BERTONI Alessandra BIO/10 All 15
2 BT035 Human physiology GROSSINI Elena, LIM Dmitry BIO/09 All 5
2 MS0743 Laboratory of cell and tissue analisys MERLIN Simone, BORSOTTI Chiara, BOLDORINI Renzo Luciano BIO/17, MED/08 All 6
2 MS0740 Molecular Biology PELICCI Giuliana, CORA' Davide BIO/11 All 6
2 MS0741 Molecular technology laboratory SANTORO Claudio Ventura, CORAZZARI Marco, GIORDANO Mara, CORRADO Lucia BIO/13, MED/03 All 7
3 MS0371 Biotechnological applications in the clinical laboratory ROLLA Roberta, CRISA' Elena, MARGIOTTA CASALUCI Gloria MED/15, MED/05 All 10
3 MS0450 Clinical Biochemistry and Clinical Molecular Biology CAPELLO Daniela BIO/12 All 5
3 BT063 Dissertation Thesis PROFIN_S All 10
3 MC117 Elective Didactic Activities NN All 12
3 MS0448 Epidemiology BARONE ADESI Francesco, FAGGIANO Fabrizio, ALLARA Elias MED/42 All 5
3 MS0120 Further Learning Activities NN All 2
3 MS0447 General Pathology CARINI Rita MED/04 All 6
3 MS0449 Pharmaceutical Chemistry PIRALI Tracey, SORBA Giovanni, MASSAROTTI Alberto CHIM/08 All 5
3 MS0432 Pharmacology and Innovation management FRESU Luigia Grazia, CONICELLA Fabrizio, FALVO Sara, TRAVELLI Cristina, SEDDIO Pasquale, JOMMI Claudio SECS-P/08, BIO/14, SECS-P/07 All 12
3 MS0446 Statistical methods for experimental studies MAGNANI Corrado MED/01 All 5
3 MS0146 Training NN All 8
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CourseFundamentals of mathematics and physics
Course IDMS0729
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderRINALDI Maurizio
TeachersPANZIERI Daniele, RINALDI Maurizio
CFU12
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year1
PeriodPrimo Semestre
Grading typeFinal grade
Teaching languageItalian
AbstractBasic mathematics, functions, derivatives, integrals. Some elements of descriptive statistics. Basic knowledge of the fundamental laws of classical physics and some elements of modern.
Reference textsSlides of the lectures published on the course web page. In addition for the module of Mathematics the book Sergio Invernizzi, Maurizio Rinaldi, Federico Comoglio, Moduli di Matematica e Statistica con l'uso di R, Zanichelli Editore, Bologna 2018.
Teaching targetsThe aim of the course is to give the students the knowledge and tools of Mathematics (differential and integral calculus) and the basic elements of Physics preparatory to the other courses of the Degree Course and to introduce students to the methodologies of analysis of experimental data (for example presentation and numerical and graphical synthesis of simple series of data). The student is expected to become able to use the knowledge acquired also in multidisciplinary field. It is expected that the student is able to apply the learned methods even in different situations and if required has the tools to expand his knowledge in an autonomous way. The student will have to acquire the ability to express the concepts fundamentals learned in a simple but clear and effective way. During the course the student should acquire the ability to study and learn by choosing your path with originality and will have to be able to choose resources, possibly even online, useful to his study.
PrerequisitesThe student should have the basic knowledge of the subject that can be acquired in a normal high school. In particular: arithmetic operations, powers, Cartesian plane, equation of the line, parabola and circumference, angles, measured in degrees and radians, circular functions, fundamental formulas of plane and solid geometry.
Didattics MethodsClassroom lectures.
Grading rulesThe exam consists of two final tests. A computer test aimed primarily at the verification of mathematical skills and a physics test consisting of a written and an oral exam where the student's ability to apply the knowledge of physics acquired during the course is evaluated.Upon reaching the sufficiency in both tests based on their outcome, the overall grade is formulated.
Full argumentsNumbers.Equation and inequalities. The Cartesian plane. Straight lines and simple curves. Linear systems. Angles and their measures: radian. Concept of mathematical functions. Domain and codomain. Image. Function composition. Invertible function and inverse function. Cosine, Sine and tangent and their inverse functions. Logarithms and exponential functions. Geometric meaning of the Derivative. Graphic computation of the derivative through repeated zooms. Approximation of the derivative at a point via Newton quotient. Three point rule. Derivative for tabulated functions. Derivative function. Differentiation rules Derivative of the sum, of the product, of the composite function, of the reciprocal and of the quotient. Derivative of the inverse function. Applications of the derivatives. Increasing and decreasing functions, Minima and maxima of functions. Computation of maxima and minima with the help of the derivative. Second derivative and study of concavity-convexity of a graph. Netwon’s method. Numerical Integration Definite integral for positive functions over finite intervals, Computation. Numerical integration with the rectangle methods and trapezoid method. Stochastic integration with the Montecarlo methods. Exact integration The Fundamental Theorem of Calculus. Indefinite integral and antiderivatives. Computation of antiderivatives. Area between two curves. Extensions of the Integral Integrals for non positive functions. Inversions of integration endpoints. . Basic elements of Statistics. Statistical data and their representations. Population and samples. Variables and observations. Graphical representations: bar charts, histograms and box-plot. Numerical representations and statistical indices. Scatter plot. Linear regression. Variable transformations to reduce to a linear relationship.Measure and vectors, motonion in one dimension- motion in a plane. The laws of the Dynamic. Work, energy and energy conservation. Conservative and non conservative forces. Rotational kinematics and dinamics. Elastic properties of materials. Static. Waves, stationary waves. Acoustics. Fluids, static and dynamics of fluids. Ideal fluids and real fluids. Surface phenomena. Temperature, Heat Propagation. Kinetic theory of gases. The Laws of Thermodynamics, thermal machines. Entropy. Electric field, Electrical current in solids, liquids, gases, Electrical circuits, Magnetic field, electromagnetic induction. Electromagnetic waves. Geometric optics, optical instruments and the eye, Elements of Physical Optics.
Expected learning objectivesKnowledge of some basic concepts and methods of mathematics, in particular: real numbers and their representations, main elementary functions, derivative and its geometric interpretation, simple integrals, calculation of areas and basic elements of Statistics. Ability at applying these concepts and methods in the modelisation of simple problems in which there are numerical and graphical presentations and synthesis of simple series of experimental data. Ability at communicating problem solutions in a clear and complete fashion. Ability in choosing the mathematical resources useful to deal with simple problems where it is required to interpret and use modeling of natural phenomena. Knowledge and understanding": Acquisition of a deep knowledge of physical phenomena, knowledge of the concepts and applications of the fundamental laws of classical physics, acquisition of appropriate scientific language. "Knowledge and understanding of applied skills": knowing how to identify the data necessary to characterize a physical phenomenon; ability to interpret data and understand orders of magnitude, ability to apply the fundamental principles and laws of physics to solve problems and describe natural phenomena. Ability to use the teaching material for a critical and reasoned study.
Modules
Course ID Course SSD Teachers
MS0730 Physics foundations FIS/01 - FISICA SPERIMENTALE PANZIERI Daniele
MS0728 Mathematics foundations MAT/04 - MATEMATICHE COMPLEMENTARI RINALDI Maurizio
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CoursePhysics foundations
Course IDMS0730
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPANZIERI Daniele
TeachersPANZIERI Daniele
CFU6
Teaching duration (hours)48
Individual study time 102
SSDFIS/01 - FISICA SPERIMENTALE
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year1
PeriodPrimo Semestre
Sites and/or partitions
Gruppo B
Gruppo A
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CoursePhysics foundations
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersPANZIERI Daniele
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractThe purpose of the course is to provide students the basic knowledge of the fundamental laws of classical physics and some elements of modern physics (applied to optics)
Reference textsSlides explained and shown during the classes and published on the course web page.
Teaching targetsThe course aims to deepen the elements of physics (optics) that may be useful for the degree and to start the students to the methodologies of analysis of experimental data.
PrerequisitesBasic knowledge of mathematics (e.g.: algebra, geometry, elementary calculus).
Didattics MethodsLectures in the classroom with slide presentations.
Other informationsLearning control with examples of closed-answer quizzes on theoretical parts.
Grading rulesThe exam consists of an oral, individual test. The oral test evaluates the student's ability to apply the knowledge acquired during the course. During the written test it is not allowed to consult notes and books. The evaluation is expressed in thirtieths and the test is passed if the score is equal to or greater than 18.
Full argumentsMeasures - scalars and vectors. Kinematics in one and two dimensions. Dynamics, Work and Energy. Rotational Kinematic and Dynamic. Fluids and elasticity. Temperature, Kinetic laws of gases, Thermodynamics laws. Electric field, current in solids, liquids and gases. Electric circuits, Magnetic field,. Electromagnetic waves. Optics and optic instruments. Diffraction and interference.
Expected learning objectivesKnowledge and understanding": Acquisition of a deep knowledge of physical phenomena, knowledge of the concepts and applications of the fundamental laws of classical physics (optics and electromagnetism), acquisition of appropriate scientific language. "Knowledge and understanding of applied skills": knowing how to identify the data necessary to characterize a physical phenomenon; ability to interpret data and understand orders of magnitude, ability to apply the fundamental principles and laws of physics to solve problems and describe natural phenomena. Ability to use the teaching material for a critical and reasoned study.
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CoursePhysics foundations
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersPANZIERI Daniele
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractThe purpose of the course is to provide students the basic knowledge of the fundamental laws of classical physics and some elements of modern physics (applied to optics)
Reference textsSlides explained and shown during the classes and published on the course web page.
Teaching targetsThe course aims to deepen the elements of physics (optics) that may be useful for the degree and to start the students to the methodologies of analysis of experimental data.
PrerequisitesBasic knowledge of mathematics (e.g.: algebra, geometry, elementary calculus).
Didattics MethodsLectures in the classroom with slide presentations.
Other informationsLearning control with examples of closed-answer quizzes on theoretical parts.
Grading rulesThe exam consists of an oral, individual test. The oral test evaluates the student's ability to apply the knowledge acquired during the course. During the written test it is not allowed to consult notes and books. The evaluation is expressed in thirtieths and the test is passed if the score is equal to or greater than 18.
Full argumentsMeasures - scalars and vectors. Kinematics in one and two dimensions. Dynamics, Work and Energy. Rotational Kinematic and Dynamic. Fluids and elasticity. Temperature, Kinetic laws of gases, Thermodynamics laws. Electric field, current in solids, liquids and gases. Electric circuits, Magnetic field,. Electromagnetic waves. Optics and optic instruments. Diffraction and interference.
Expected learning objectivesKnowledge and understanding": Acquisition of a deep knowledge of physical phenomena, knowledge of the concepts and applications of the fundamental laws of classical physics (optics and electromagnetism), acquisition of appropriate scientific language. "Knowledge and understanding of applied skills": knowing how to identify the data necessary to characterize a physical phenomenon; ability to interpret data and understand orders of magnitude, ability to apply the fundamental principles and laws of physics to solve problems and describe natural phenomena. Ability to use the teaching material for a critical and reasoned study.
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CourseMathematics foundations
Course IDMS0728
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderRINALDI Maurizio
TeachersRINALDI Maurizio
CFU6
Teaching duration (hours)48
Individual study time 102
SSDMAT/04 - MATEMATICHE COMPLEMENTARI
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year1
PeriodPrimo Semestre
Sites and/or partitions
Gruppo A
Gruppo B
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CourseMathematics foundations
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderRINALDI Maurizio
TeachersRINALDI Maurizio
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractBasic mathematics, functions, derivatives, integrals. Some elements of descriptive statistics.
Reference textsSergio Invernizzi, Maurizio Rinaldi, Federico Comoglio, Moduli di Matematica e Statistica con l'uso di R, Zanichelli Editore, Bologna 2018
Teaching targets*Knowledge and understanding. The course aims at providing student with the knowledge and the ability to manage the mathematical tools required to understand and follow the other courses of the Degree and at introducing her/him to the numerical and graphical presentation and synthesis of simple series of experimental data and to the modeling of natural phenomena. More specifically the course aims at providing students with basic methods of differential and integral calculus needed to understand mathematical models. *Applying knowledge and understanding Students are expected to be able to use the acquired skills even in a multidisciplinary context and they should be able to resort to mathematics and statistics even in situations external to the course. *Making judgements. At the end of the course students are expected to apply learned methods even in different situations and that they have acquired the tools needed to extend their knowledge by themselves. *Communication skills. At the end of the course students are expected to be able to express the learned concepts in a clear way. *Learning skills. During the course students should learn how to study by choosing their personal path and should become able to choose the appropriate resources, possibly online.
PrerequisitesThe student should have the basic knowledge of the subject that can be acquired in a normal high school. In particular: arithmetic operations, powers, Cartesian plane, equation of the line, parabola and circumference, angles, measured in degrees and radians, circular functions, fundamental formulas of plane and solid geometry.
Didattics MethodsLectures are given in the classroom.
Other informationsReference material and further details are provided on the DIR website [https://www.dir.uniupo.it/ ] . Enrolment key is provided during the lectures.
Grading rulesOngoing Quizzes and online Final Examination. The ongoing quizzes are assigned periodically at the end of a given subject. The Final focuses on all the course matters. Final evaluation gives a weight of 20% to the ongoing quizzes and 80% to the Final. Ongoing quizzes could be replaced by an oral examination
Full arguments0.1 Numbers 0.2. Equation and inequalities 0.3. The Cartesian plane. Straight lines and simple curves. Linear systems. 0.4 Angles and their measures: radian. 1.1. Concept of mathematical functions. Domain and codomain. Image. Function composition. Invertible function and inverse function. 1.2. Cosine, Sine and tangent and their inverse functions. Logarithms and exponential functions. 2.1 Geometric meaning of the Derivative. Graphic computation of the derivative through repeated zooms. Approximation of the derivative at a point via Newton quotient. Three point rule. Derivative for tabulated functions. Derivative function. 2.2 Differentiation rules Derivative of the sum, of the product, of the composite function, of the reciprocal and of the quotient. Derivative of the inverse function. Applications of the derivatives 2.3 Increasing and decreasing functions, Minima and maxima of functions. Computation of maxima and minima with the help of the derivative. Second derivative and study of concavity-convexity of a graph. Netwon’s method. 3.1 Numerical Integration Definite integral for positive functions over finite intervals, Computation. 3.2. Numerical integration with the rectangle methods and trapezoid method. Stochastic integration with the Montecarlo methods. Exact integration The Fundamental Theorem of Calculus. Indefinite integral and antiderivatives. Computation of antiderivatives. 3.3. Area between two curves. Extensions of the Integral Integrals for non positive functions. Inversions of integration endpoints. 4.1. Basic elements of Statistics. Statistical data and their representations. Population and samples. Variables and observations. Graphical representations: bar charts, histograms and box-plot. Numerical representations and statistical indices. Scatter plot. Linear regression. Variable transformations to reduce to a linear relationship.
Expected learning objectivesKnowledge of some basic concepts and methods of mathematics, in particular: real numbers and their representations, main elementary functions, derivative and its geometric interpretation, simple integrals, calculation of areas and basic elements of Statistics. Ability at applying these concepts and methods in the modelisation of simple problems in which there are numerical and graphical presentations and synthesis of simple series of experimental data. Ability at communicating problem solutions in a clear and complete fashion. Ability in choosing the mathematical resources useful to deal with simple problems where it is required to interpret and use modeling of natural phenomena.
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CourseMathematics foundations
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderRINALDI Maurizio
TeachersRINALDI Maurizio
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractBasic mathematics, functions, derivatives, integrals. Some elements of descriptive statistics.
Reference textsSergio Invernizzi, Maurizio Rinaldi, Federico Comoglio, Moduli di Matematica e Statistica con l'uso di R, Zanichelli Editore, Bologna 2018
Teaching targets*Knowledge and understanding. The course aims at providing student with the knowledge and the ability to manage the mathematical tools required to understand and follow the other courses of the Degree and at introducing her/him to the numerical and graphical presentation and synthesis of simple series of experimental data and to the modeling of natural phenomena. More specifically the course aims at providing students with basic methods of differential and integral calculus needed to understand mathematical models. *Applying knowledge and understanding Students are expected to be able to use the acquired skills even in a multidisciplinary context and they should be able to resort to mathematics and statistics even in situations external to the course. *Making judgements. At the end of the course students are expected to apply learned methods even in different situations and that they have acquired the tools needed to extend their knowledge by themselves. *Communication skills. At the end of the course students are expected to be able to express the learned concepts in a clear way. *Learning skills. During the course students should learn how to study by choosing their personal path and should become able to choose the appropriate resources, possibly online.
PrerequisitesThe student should have the basic knowledge of the subject that can be acquired in a normal high school. In particular: arithmetic operations, powers, Cartesian plane, equation of the line, parabola and circumference, angles, measured in degrees and radians, circular functions, fundamental formulas of plane and solid geometry.
Didattics MethodsLectures are given in the classroom.
Other informationsReference material and further details are provided on the DIR website [https://www.dir.uniupo.it/ ] . Enrolment key is provided during the lectures or given upon request..
Grading rulesOngoing Quizzes and online Final Examination. The ongoing quizzes are assigned periodically at the end of a given subject. The Final focuses on all the course matters. Final evaluation gives a weight of 20% to the ongoing quizzes and 80% to the Final. Ongoing quizzes could be replaced by an oral examination
Full arguments0.1 Numbers 0.2. Equation and inequalities 0.3. The Cartesian plane. Straight lines and simple curves. Linear systems. 0.4 Angles and their measures: radian. 1.1. Concept of mathematical functions. Domain and codomain. Image. Function composition. Invertible function and inverse function. 1.2. Cosine, Sine and tangent and their inverse functions. Logarithms and exponential functions. 2.1 Geometric meaning of the Derivative. Graphic computation of the derivative through repeated zooms. Approximation of the derivative at a point via Newton quotient. Three point rule. Derivative for tabulated functions. Derivative function. 2.2 Differentiation rules Derivative of the sum, of the product, of the composite function, of the reciprocal and of the quotient. Derivative of the inverse function. Applications of the derivatives 2.3 Increasing and decreasing functions, Minima and maxima of functions. Computation of maxima and minima with the help of the derivative. Second derivative and study of concavity-convexity of a graph. Netwon’s method. 3.1 Numerical Integration Definite integral for positive functions over finite intervals, Computation. 3.2. Numerical integration with the rectangle methods and trapezoid method. Stochastic integration with the Montecarlo methods. Exact integration The Fundamental Theorem of Calculus. Indefinite integral and antiderivatives. Computation of antiderivatives. 3.3. Area between two curves. Extensions of the Integral Integrals for non positive functions. Inversions of integration endpoints. 4.1. Basic elements of Statistics. Statistical data and their representations. Population and samples. Variables and observations. Graphical representations: bar charts, histograms and box-plot. Numerical representations and statistical indices. Scatter plot. Linear regression. Variable transformations to reduce to a linear relationship.
Expected learning objectivesKnowledge of some basic concepts and methods of mathematics, in particular: real numbers and their representations, main elementary functions, derivative and its geometric interpretation, simple integrals, calculation of areas and basic elements of Statistics. Ability at applying these concepts and methods in the modelisation of simple problems in which there are numerical and graphical presentations and synthesis of simple series of experimental data. Ability at communicating problem solutions in a clear and complete fashion. Ability in choosing the mathematical resources useful to deal with simple problems where it is required to interpret and use modeling of natural phenomena.
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CourseGenetics
Course IDMS0734
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGIORDANO Mara
TeachersPERSICHETTI Francesca, SOLURI Angela Maria Felicia, GIORDANO Mara, CORRADO Lucia, BARIZZONE Nadia
CFU9
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year1
PeriodSecondo Semestre
Grading typeFinal grade
Modules
Course ID Course SSD Teachers
MS0735 Genetics of microorganisms and eukaryotic BIO/18 - GENETICA PERSICHETTI Francesca, SOLURI Angela Maria Felicia
MS0736 Human Genetics MED/03 - GENETICA MEDICA GIORDANO Mara, CORRADO Lucia, BARIZZONE Nadia
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CourseGenetics of microorganisms and eukaryotic
Course IDMS0735
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPERSICHETTI Francesca
TeachersPERSICHETTI Francesca, SOLURI Angela Maria Felicia
CFU4
Teaching duration (hours)32
Individual study time 68
SSDBIO/18 - GENETICA
Course typeModulo di sola Frequenza
Course categoryC - Affine o integrativo
Year1
Sites and/or partitions
Gruppo A
Gruppo B
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CourseGenetics of microorganisms and eukaryotic
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderSOLURI Angela Maria Felicia
TeachersPERSICHETTI Francesca, SOLURI Angela Maria Felicia
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageitalian
AbstractThe course provides knowledge on the structure, organization, replication and transmission of the hereditary material in prokaryotes and eukaryotes.
Reference textsRUSSEL P.J. Genetica “un approccio molecolare”, Ed. Pearson SNUSTAD P.D., SIMMONS M.J.- Principi di genetica, Ed. EdiSES. GRIFFITHS A.J.F., GELBART W., LEWONTIN R.C., SUZUKI D.T., MILLER J.H., WESSLER S.R. Genetica - Principi di analisi formale, Ed. Zanichelli.
Teaching targetsThe course aims to teach the student the organization, expression and transmission of genetic material, both in prokaryotes and eukaryotes. Student will be provided of the tools to understand and recognize the different mode of transmission of genetic traits, with particular emphasis to the Mendelian inheritance and its extensions.
PrerequisitesBasic knowledge of biology.
Didattics MethodsClass lectures with PowerPoint projections and use of the overhead projector for exercises explanation and solution.
Other informationsLearning control: In-class writing exercises
Grading rulesWritten test. The test consists of 31 multiple choice questions (5 options, only one correct) both theoretical on the different topics of the program, and simple problems of formal genetics. The questions will receive a score of 1 if the answer is correct and zero if the answer is incorrect or void. The minimum score to pass the exam is 18 points. The honors will be awarded to students who receive a total score of more than 30.
Full argumentsThe genetic material: chemical composition and structure. Replication, transcription and translation of genetic material. DNA organization in prokaryotic and eukaryotic chromosomes. Mutation and DNA repair. Cell division: mitosis and meiosis. Mendel's laws and their applications. Extension of mendelian analysis: multiple alleles and genetic interaction (epistasis, penetrance and expressivity). Genetic recombination. Genetics of microorganisms: bacteria, bacteriophages and yeast. Exchange of genetic material: conjugation, transformation and transduction. Regulation of gene expression in bacteria and bacteriophages.
Expected learning objectivesKnowledge and understanding To know the structure, replication and transmission of the genetic material. To understand the bases of the Mendelian inheritance and of the interaction among genes. To know the most important mechanisms of transferring of genetic material and regulation of gene expression in prokaryotes. Applying knowledge and understanding: At the end of the course the student should be able: To apply the acquired knowledge to solve questions regarding the Mendelian inheritance, both qualitatively (expected genotypes and phenotypes) and quantitatively (expected frequencies). To distinguish among the different types of inheritance and to predict the phenotypic effects of the interactions between alleles at a single locus and among genes. To describe and compare the different mode of transferring genetic information between bacteria. Making judgements Ability to formulate hypotheses about genetic transmission and to critically discuss the complex relationships between genotype and phenotype. Communication skills Ability to expose the subjects of the course in a clear and comprehensible way and with the use of the appropriate scientific terminology. Learning skills Ability to deepen their knowledge of the genetics on several books and on scientific articles in order to follow with a critical attitude the advancement of discipline.
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CourseGenetics of microorganisms and eukaryotic
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersPERSICHETTI Francesca
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractThe course provides knowledge on the structure, organization, replication and transmission of the hereditary material in prokaryotes and eukaryotes.
Reference textsRUSSEL P.J. Genetica “un approccio molecolare”, Ed. Pearson SNUSTAD P.D., SIMMONS M.J.- Principi di genetica, Ed. EdiSES. GRIFFITHS A.J.F., GELBART W., LEWONTIN R.C., SUZUKI D.T., MILLER J.H., WESSLER S.R. Genetica - Principi di analisi formale, Ed. Zanichelli.
Teaching targetsThe course aims to teach the student the organization, expression and transmission of genetic material, both in prokaryotes and eukaryotes. Student will be provided of the tools to understand and recognize the different mode of transmission of genetic traits, with particular emphasis to the Mendelian inheritance and its extensions.
PrerequisitesBasic knowledge of biology.
Didattics MethodsClass lectures with PowerPoint projections and use of the overhead projector for exercises explanation and solution.
Other informationsLearning control: In-class writing exercises
Grading rulesWritten test. The test consists of 31 multiple choice questions (5 options, only one correct) both theoretical on the different topics of the program, and simple problems of formal genetics. The questions will receive a score of 1 if the answer is correct and zero if the answer is incorrect or void. The minimum score to pass the exam is 18 points. The honors will be awarded to students who receive a total score of more than 30.
Full argumentsThe genetic material: chemical composition and structure. Replication, transcription and translation of genetic material. DNA organization in prokaryotic and eukaryotic chromosomes. Mutation and DNA repair. Cell division: mitosis and meiosis. Mendel's laws and their applications. Extension of mendelian analysis: multiple alleles and genetic interaction (epistasis, penetrance and expressivity). Genetic recombination. Genetics of microorganisms: bacteria, bacteriophages and yeast. Exchange of genetic material: conjugation, transformation and transduction. Regulation of gene expression in bacteria and bacteriophages.
Expected learning objectivesKnowledge and understanding To know the structure, replication and transmission of the genetic material. To understand the bases of the Mendelian inheritance and of the interaction among genes. To know the most important mechanisms of transferring of genetic material and regulation of gene expression in prokaryotes. Applying knowledge and understanding: At the end of the course the student should be able: To apply the acquired knowledge to solve questions regarding the Mendelian inheritance, both qualitatively (expected genotypes and phenotypes) and quantitatively (expected frequencies). To distinguish among the different types of inheritance and to predict the phenotypic effects of the interactions between alleles at a single locus and among genes. To describe and compare the different mode of transferring genetic information between bacteria. Making judgements Ability to formulate hypotheses about genetic transmission and to critically discuss the complex relationships between genotype and phenotype. Communication skills Ability to expose the subjects of the course in a clear and comprehensible way and with the use of the appropriate scientific terminology. Learning skills Ability to deepen their knowledge of the genetics on several books and on scientific articles in order to follow with a critical attitude the advancement of discipline.
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CourseHuman Genetics
Course IDMS0736
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGIORDANO Mara
TeachersGIORDANO Mara, CORRADO Lucia, BARIZZONE Nadia
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/03 - GENETICA MEDICA
Course typeModulo di sola Frequenza
Course categoryB - Caratterizzante
Year1
Sites and/or partitions
Gruppo A
Gruppo B
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CourseHuman Genetics
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGIORDANO Mara
TeachersGIORDANO Mara
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractStructure and evolution of the human genome. - Cell division: mitosis and meiosis. -Correlation genotype-phenotype (example: ABO blood group, sickle-cell anemia). -Principles of Mendelian genetics. - Exceptions to the laws of Mendel. - Chromosomal classification criteria and chromosomal aberrations - Population genetics. -Transmission of independent and concatenated characters. Linkage analysis. - Quantitative and semi-quantitative multifactorial traits. - Methods of molecular genetics - Analysis of genomic databases
Reference textsEredità, Principi e problematiche della genetica umana, M.R. Cummings, Edizioni EdiSES, II edizione (2009) Genetica, Peter J. Russel, Edizioni EdiSES, II edizione Genetica Umana e Medica Edizioni EDRA Masson 2014 (III edizione) Giovanni Neri e Maurizio Genuardi
Teaching targetsTo understand the relationships between genetic information and the phenotype. To Know how to evaluate the risk of recurrence of genetic diseases or with genetic components in families. To know the molecular basis of genetically based diseases. Apply knowledge to the analysis and resolution of problems of formal and molecular genetics. To apply the basic methods of molecular genetics such as PCR and sequencing through practical laboratory activities
PrerequisitesFundamentals of biochemistry, cell biology and statistics.
Didattics MethodsLectures with presentations in MS-Power Point format.
Grading rulesThe knowledge will be verified by written exam with open and / or multiple choice questions. For most questions, the answer will require the solving of problems
Full argumentsDNA structure. Organization of DNA in chromosomes. Structure and function of the chromosome. -The Human Genome structure. Organization of genes. Transposable elements. Ripetitive DNA sequences. Gene families. -Cell division: mitosis and meiosis. -Genotype-phenotype correlation (example: ABO blood group, sickle cell anemia). Protein translation and genetic code. Correlation between type of mutations and dominant or recessive phenotype at different levels of phenotype investigation. Genetic consequences of meiosis. -Principles of Mendelian genetics. Transmission in families of autosomal recessive, dominant and X-linked monofactorial traits; recurrence risks in families. -"Exceptions" to Mendel’s rules. Incomplete penetrance, variable expressivity, genetic heterogeneity. X-chromosome inactivation, mitochondrial inheritance, genomic imprinting -Criteria for classification of chromosomes and methods for their identification. Chromosomal aberrations and their incidence at birth; Reproductive risks associated with chromosomal aberrations. -Population genetics. Gene and genotypic frequencies in the population and Hardy-Weinberg equilibrium. -Evolution of human populations. Evolutionary forces (mutation, selection, genetic drift, migration) that influence allelic and genotype frequencies. -Transmission of independent and concatenated characters. Localization of genes on chromosomes based on their transmission in families (linkage analysis). Different methods for gene mapping. -Multi-factorial characters. Population distribution of multifactorial characters; evaluation of the weight of the genetic component in multifactorial disorders; identification of susceptibility genes in multifactorial diseases. -DNA investigation methods. Polymerase Chain Reaction (PCR); Analysis of VNTR, STR and fingerprint polymorphisms; DNA sequencing; Methods for the identification of nucleotide variations. Genomic analysis through next generation sequencing methods and chromosomal microarray (MCA). -Analysis of Genomic Databases
Expected learning objectivesAt the end of the course, students should be able to: -Understand the relationships between genetic information and the phenotype. - Know how to evaluate the risk of recurrence of genetic diseases or with genetic components in families. -Know the molecular basis of genetically based diseases. -Apply knowledge to the analysis and resolution of problems of formal and molecular genetics.
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CourseHuman Genetics
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCORRADO Lucia
TeachersCORRADO Lucia, BARIZZONE Nadia
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractStructure and evolution of the human genome. - Cell division: mitosis and meiosis. -Correlation genotype-phenotype (example: ABO blood group, sickle-cell anemia). -Principles of Mendelian genetics. - Exceptions to the laws of Mendel. - Chromosomal classification criteria and chromosomal aberrations - Population genetics. -Transmission of independent and concatenated characters. Linkage analysis. - Quantitative and semi-quantitative multifactorial traits. - Methods of molecular genetics - Analysis of genomic databases
Reference textsEredità, Principi e problematiche della genetica umana, M.R. Cummings, Edizioni EdiSES, II edizione (2009) Genetica in medicina Robert L. Nussbaum, Roderick R. McInnes, Huntington F. Willard Thompson & Thompson. Edises, 2018
Teaching targetsTo understand the relationships between genetic information and the phenotype. To Know how to evaluate the risk of recurrence of genetic diseases or with genetic components in families. To know the molecular basis of genetically based diseases. Apply knowledge to the analysis and resolution of problems of formal and molecular genetics.
PrerequisitesFundamentals of biochemistry, cell biology and statistics.
Didattics MethodsLectures with presentations in MS-Power Point format.
Grading rulesThe knowledge will be verified by written exam with open and / or multiple choice questions. For most questions, the answer will require the solving of problems
Full argumentsDNA structure. Organization of DNA in chromosomes. Structure and function of the chromosome. -The Human Genome structure. Organization of genes. Transposable elements. Ripetitive DNA sequences. Gene families. -Cell division: mitosis and meiosis. -Genotype-phenotype correlation (example: ABO blood group, sickle cell anemia). Protein translation and genetic code. Correlation between type of mutations and dominant or recessive phenotype at different levels of phenotype investigation. Genetic consequences of meiosis. -Principles of Mendelian genetics. Transmission in families of autosomal recessive, dominant and X-linked monofactorial traits; recurrence risks in families. -"Exceptions" to Mendel’s rules. Incomplete penetrance, variable expressivity, genetic heterogeneity. X-chromosome inactivation, mitochondrial inheritance, genomic imprinting -Criteria for classification of chromosomes and methods for their identification. Chromosomal aberrations and their incidence at birth; Reproductive risks associated with chromosomal aberrations. -Population genetics. Gene and genotypic frequencies in the population and Hardy-Weinberg equilibrium. -Evolution of human populations. Evolutionary forces (mutation, selection, genetic drift, migration) that influence allelic and genotype frequencies. -Transmission of independent and concatenated characters. Localization of genes on chromosomes based on their transmission in families (linkage analysis). Different methods for gene mapping. -Multi-factorial characters. Population distribution of multifactorial characters; evaluation of the weight of the genetic component in multifactorial disorders; identification of susceptibility genes in multifactorial diseases. -DNA investigation methods. Polymerase Chain Reaction (PCR); Analysis of VNTR, STR and fingerprint polymorphisms; DNA sequencing; Methods for the identification of nucleotide variations. Genomic analysis through next generation sequencing methods and chromosomal microarray (MCA). -Analysis of Genomic Databases
Expected learning objectivesAt the end of the course, students should be able to: -Understand the relationships between genetic information and the phenotype. - Know how to evaluate the risk of recurrence of genetic diseases or with genetic components in families. -Know the molecular basis of genetically based diseases. -Apply knowledge to the analysis and resolution of problems of formal and molecular genetics.
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CourseHistology and anatomy
Course IDMS0731
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPRAT Maria Giovanna
TeachersBOZZO Chiarella, COTELLA Diego, FOLLENZI Antonia, PRAT Maria Giovanna, BOCCAFOSCHI Francesca
CFU15
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year1
PeriodAnnuale
Grading typeFinal grade
Teaching languageItalian
AbstractStudy of the characteristics of eukaryotic cells (Biology of the Eukariotic Cells), of the tissues (Histology), and of the organs and systems of the human body (Anatomy). Study of the first periods of the development of the human being, of the mechanisms of their origin in the embryo and the interaction of the tissues to form organs. Stem cells from the beginning in the embryo to the adult. Animal models used to study developmental biology.
Reference textsAlberts, Bray, Hopkin, Johnson, Lewis et al BIOLOGIA MOLECOLARE DELLA CELLULA (l'essenziale) ed. Zanichelli Ginelli, Malcovati Molecole, Cellule e Organismi. ed. EdiSES Wheater: Istologia e Anatomia microscopica, CEA Stevens-Lowe: Istologia Umana, CEA Barbieri-Carinci: Embriologia, III ed. CEA Saladin: Anatomia Umana Casa editrice PICCIN
Teaching targetsAt the end of the course the student must: - have acquired the basic knowledge on the structure and organization of the eukariotc cell, the molecular and cellular components of the tissues, their organization in organs and systems. Moreover, of the embryological origin of the different tissues of the human body - have acquired the knowledge about tissue and organ homeostasis - have acquired basic knowledge on the first steps of the embryonic development (the first two months in humans) and of some animal models used for the study of embryonic development
PrerequisitesThe student must be in possession of biology and genetics concepts, such as those offered at the high school.
Didattics MethodsDirect lessons in classroom with the use of files in Presentation format and videos.
Other informationsVideo projector and computers with Internet connection supplied to the classroom
Grading rulesThe exam consists of a multichoise written proof . Registration is mandatory. Only correct answers are considered for the final vote.
Full argumentsThe program is referred in the single three courses
Expected learning objectivesIn order to reach the knowledge and skills corresponding to the minimumlevel of sufficiency, the student is asked to demonstrate: (KNOWLEDGE) -to know the structure, organization and function of the eukaryotic cell; -to understand the molecular mechanisms that govern the flow of gene information from DNA to proteins; to know the structure of the cells and extracellular matrix organization and their role in the formation of the different tissues; the organization of the latter in the different organs and systems/apparatus; to know the morphologic properties of the tissues and their correlation with function; to know the macroscopic anatomy of the human body. (SKILLS) Ability to understand the basics of cellular and tissue testing and methods for their study. (Additional SKILLS) - to be able to read, understand and comment scientific concepts from textbooks or specialized literature - to know, or to be able to trace, the essential terminology. - to be able to evaluate the didactics delivered.
Modules
Course ID Course SSD Teachers
MS0002 Eukaryotic cell biology BIO/13 - BIOLOGIA APPLICATA COTELLA Diego, BOZZO Chiarella
MS0732 Histology and developmental biology BIO/17 - ISTOLOGIA PRAT Maria Giovanna, FOLLENZI Antonia
BT015 Human Anatomy BIO/16 - ANATOMIA UMANA BOCCAFOSCHI Francesca
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CourseEukaryotic cell biology
Course IDMS0002
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCOTELLA Diego
TeachersBOZZO Chiarella, COTELLA Diego
CFU5
Teaching duration (hours)40
Individual study time 85
SSDBIO/13 - BIOLOGIA APPLICATA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year1
PeriodPrimo Semestre
Sites and/or partitions
Gruppo B
Gruppo A
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CourseEukaryotic cell biology
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBOZZO Chiarella
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractProgramma dettagliato del corso di Biologia della Cellula Eucariotica (MS002) A partire da A.A. 2017-18 Docente del corso: Dr.ssa Chiarella Bozzo Corso di laurea in Biotecnologie Struttura ed organizzazione della cellula • Definizione di essere vivente • La cellula eucariotica e la cellula procariotica • I compartimenti cellulari. • I lipidi come costituenti di barriere chimiche Le membrane biologiche • I lipidi delle membrane biologiche: fosfolipidi, glicolipidi e colesterolo • L'organizzazione a doppio strato o a micella • La fluidità' del doppio strato fosfolipidico: ruolo dei grassi insaturi • Le proteine intrinseche e proteine estrinseche • La teoria del Mosaico fluido I meccanismi di trasporto attraverso le membrane • Caratteristiche di permeabilità del doppio strato lipidico • Diffusione semplice e diffusione facilitata • Trasporto passivo e trasporto attivo • Proteine canale e proteine trasportatrici: caratteristiche cinetiche dei due sistemi e modalità di funzionamento • Trasportatore del glucosio • Pompa Na+/K+ ATPasi: modalità di funzionamento • Antiporto e simporto • Canali attivati da ligando e canali attivati da voltaggio • ll potenziale di membrana e il potenziale d’azione Mitocondri e la sintesi di ATP • Nozioni generali sul processo di glicolisi, ossidazione del piruvato, ciclo di Krebs e catena di trasporto degli elettroni • La struttura del mitocondrio • Il genoma mitocondriale • L’origine evolutiva del mitocondrio • Il trasporto delle proteine nel mitocondrio Reticolo endoplasmatico rugoso e apparato di Golgi • Sintesi delle proteine di membrana e di secrezione • Glicosilazione e maturazione delle proteine nel Golgi • Traffico vescicolare: formazione di vescicole, movimento delle vescicole, riconoscimento del organello bersaglio • Esocitosi Meccanismi di indirizzamento delle proteine nei compartimenti cellulari • Sequenze segnale e localizzazione delle proteine (entrata nel nucleo, nel reticolo endoplasmatico, nel mitocondrio) Endocitosi • Pinocitosi, fagocitosi e endocitosi mediata da recettore Degradazione delle macromolecole biologiche nella cellula • Il lisosoma • Il proteosoma I perossisomi Il citoscheletro • Microfilamenti contrattili di actina e miosina e il movimento cellulare • Filamenti intermedi • Microtubuli ed il movimento degli organelli • La contrazione muscolare La matrice extracellulare • I collageni, laminine le fibronectine, i proteoglicani • Le proteine della matrice extracellulare stabiliscono interazioni multiple • L'interazione matrice-cellula è mediata da recettori specifici: le integrine • Struttura e funzione delle integrine e delle caderine Il ciclo cellulare e la mitosi • Il ciclo cellulare G1, S, G2 ed M. • Il ruolo delle cicline e delle cdk nella progressione del ciclo cellulare • I check point del ciclo cellulare: check point di G1 (Rb/E2F), di G2 (p53) e check point mitotico (cdc20/APC). Morte cellulare e apoptosi • Necrosi • Apoptosi: la via intrinseca (p53 e il mitocondrio) e la via estrinseca (i recettori di morte) Flusso dell’informazione genica: la definizione di gene nell’era ENCODE • Replicazione del DNA e mantenimento della sua integrità • Trascrizione del DNA: sintesi degli RNA e loro maturazione • Influenza dell’ambiente sul flusso genico: Epigenetica • Stabilità degli RNA: l’interferenza
Reference textsAlberts, Bray, Hopkin, Johnson, Lewis et al BIOLOGIA MOLECOLARE DELLA CELLULA (l'essenziale) ed. Zanichelli Ginelli, Malcovati MOLECOLE, CELLULE E ORGANISMI ed. EdiSES Karp BIOLOGIA CELLULARE E MOLECOLARE ed. EDISES Cooper, Hausman LA CELLULA - UN APPROCCIO MOLECOLARE ed. Piccin
Teaching targetsIl modulo ha l’obiettivo di fornire le nozioni essenziali di biologia cellulare e molecolare che forniranno allo studente gli strumenti necessari per arrivare a conoscere e descrivere in modo organico la struttura di cellule eucariotiche e le loro funzioni.
PrerequisitesLo studente deve essere in possesso delle nozioni basilari di biologia e chimica.
Didattics MethodsLezioni frontali. Presentazioni in formato MS-Power Point, siti internet didattici.
Grading rulesProva scritta con domande a scelta multipla.
Full argumentsProgramma dettagliato del corso di Biologia della Cellula Eucariotica (MS002) A partire da A.A. 2017-18 Docente del corso: Dr.ssa Chiarella Bozzo Corso di laurea in Biotecnologie Struttura ed organizzazione della cellula • Definizione di essere vivente • La cellula eucariotica e la cellula procariotica • I compartimenti cellulari. • I lipidi come costituenti di barriere chimiche Le membrane biologiche • I lipidi delle membrane biologiche: fosfolipidi, glicolipidi e colesterolo • L'organizzazione a doppio strato o a micella • La fluidità' del doppio strato fosfolipidico: ruolo dei grassi insaturi • Le proteine intrinseche e proteine estrinseche • La teoria del Mosaico fluido I meccanismi di trasporto attraverso le membrane • Caratteristiche di permeabilità del doppio strato lipidico • Diffusione semplice e diffusione facilitata • Trasporto passivo e trasporto attivo • Proteine canale e proteine trasportatrici: caratteristiche cinetiche dei due sistemi e modalità di funzionamento • Trasportatore del glucosio • Pompa Na+/K+ ATPasi: modalità di funzionamento • Antiporto e simporto • Canali attivati da ligando e canali attivati da voltaggio • ll potenziale di membrana e il potenziale d’azione Mitocondri e la sintesi di ATP • Nozioni generali sul processo di glicolisi, ossidazione del piruvato, ciclo di Krebs e catena di trasporto degli elettroni • La struttura del mitocondrio • Il genoma mitocondriale • L’origine evolutiva del mitocondrio • Il trasporto delle proteine nel mitocondrio Reticolo endoplasmatico rugoso e apparato di Golgi • Sintesi delle proteine di membrana e di secrezione • Glicosilazione e maturazione delle proteine nel Golgi • Traffico vescicolare: formazione di vescicole, movimento delle vescicole, riconoscimento del organello bersaglio • Esocitosi Meccanismi di indirizzamento delle proteine nei compartimenti cellulari • Sequenze segnale e localizzazione delle proteine (entrata nel nucleo, nel reticolo endoplasmatico, nel mitocondrio) Endocitosi • Pinocitosi, fagocitosi e endocitosi mediata da recettore Degradazione delle macromolecole biologiche nella cellula • Il lisosoma • Il proteosoma I perossisomi Il citoscheletro • Microfilamenti contrattili di actina e miosina e il movimento cellulare • Filamenti intermedi • Microtubuli ed il movimento degli organelli • La contrazione muscolare La matrice extracellulare • I collageni, laminine le fibronectine, i proteoglicani • Le proteine della matrice extracellulare stabiliscono interazioni multiple • L'interazione matrice-cellula è mediata da recettori specifici: le integrine • Struttura e funzione delle integrine e delle caderine Il ciclo cellulare e la mitosi • Il ciclo cellulare G1, S, G2 ed M. • Il ruolo delle cicline e delle cdk nella progressione del ciclo cellulare • I check point del ciclo cellulare: check point di G1 (Rb/E2F), di G2 (p53) e check point mitotico (cdc20/APC). Morte cellulare e apoptosi • Necrosi • Apoptosi: la via intrinseca (p53 e il mitocondrio) e la via estrinseca (i recettori di morte) Flusso dell’informazione genica: la definizione di gene nell’era ENCODE • Replicazione del DNA e mantenimento della sua integrità • Trascrizione del DNA: sintesi degli RNA e loro maturazione • Influenza dell’ambiente sul flusso genico: Epigenetica • Stabilità degli RNA: l’interferenza
Expected learning objectivesStruttura e funzione della cellula eucariotica.
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CourseEukaryotic cell biology
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersCOTELLA Diego
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractThis course deals with the biology of the eukaryotic cell: structure, function and biosynthesis of cell membranes and organelles; the cytoskeleton and the extracellular matrix. Chromatin structure, the synthesis of RNA and protein and gene flow of information.
Reference textsAlberts, Johnson, et al BIOLOGIA MOLECOLARE DELLA CELLULA ed. Zanichelli Ginelli, Malcovati MOLECOLE, CELLULE E ORGANISMI. ed. EdiSES
Teaching targetsThis course contributes to the training objectives of the morpho-functional scope of the degree courses in Biotechnology and Biological Sciences, providing knowledge and skills at the cellular and tissue level that are preparatory to all other biological teachings and that can be used in all professional fields of the graduates in Biotechnology (class L-2) or in Biological Sciences (class L-13), as well as for the continuation of studies in the master's degrees in medical, veterinary and pharmaceutical biotechnologies (LM-9 class) biology (CLM-06) or related master's degrees.
PrerequisitesThe student must be in possession of basic concepts of chemistry, biology and genetics, such as those offered at the high school.
Didattics MethodsTeaching methods include: lectures, active learning in the classroom and at distance. • Frontal lessons and classroom activities *: o Magistral lectures supported by presentations (ppt) with graphic illustrations, mind maps, optical and electronic microscopy photographs, animation films of cellular processes o Classroom activities * with active student participation (representation of cellular processes, instant polls, termination of exercises). • Activities and online material (moodle **): o Educational material presented in class. o Video recordings replacing the lectures o Quiz, forums and workshops for learning and self-assessment o Optional material for further information (*) possession of smartphone, notebook or tablet recommended for classroom interruption via WIFI of the University. (**) activities on Moodle are tracked and progress in activities subject to the passing of specific conditions, then customized for each student.
Other informationsAttività di supporto Tutorato: • Gi studenti possono porre domande e trovare un sostegno allo studio attraverso il forum dell'insegnamento su moodle • Esempi di prove di esame sono forniti nelle attività online su moodle. Students with disorders that may affect learning (eg students color blind, visually impaired, hearing impaired, dyslexic or with physical disabilities) are encouraged to contact teachers to adapt learning materials, activities and test mode. Workers and students that for other reasons are unable to attend the course are encouraged to contact teachers to determine how to achieve the expected outcomes.
Grading rulesONLINE MONITORING: the activities tracked take place on the Moodle platform and consist of watching videos, animations and other educational material, self-learning and verification quizzes, forums, workshops and other activities. The progress of each student's activities is monitored automatically. The online activities are preparatory to the in itinere test. MONITORING IN ITINERIES: The in itinere test focuses on the first module of the course, ie the biology of the cell, and is recommended but not mandatory. The test allows to acquire a score that will constitute the average score of the appeals to which the student is presented in the sessions of June-July-September. METHODS OF CARRYING OUT THE TESTS IN ITINERIES AND FINALS: The in itinere test and the final exam are written tests, of a respective duration of 40 and 120 minutes for a total of 40 and 120 questions, and focus on topics of the program both of the lessons. The questions, which aim to verify the achievement of the expected learning outcomes, are of the typology: multiple choice, recognition of cellular and tissue structures in optical and electronic microscopy (peer the part of histology), open questions for the description of biological structures and phenomena. CALENDAR AND ENROLLMENT: The calendar of final exam sessions and of the ongoing exam are published on the University portal. The students' attention is drawn to the fact that the enrollment in the in itinere test and the appeals closes within the date of admission indicated on the portal and as regards registration for the exam sessions, is subject to the completion of the student opinion questionnaire.
Full argumentsStructure and organization of the cell • Definition life • The eukaryotic cell and prokaryotic cell • The chemistry of the cell: water and macromolecules (carbohydrates, lipids, protein and nucleic acids) • The cell compartments The Biological membranes • The lipids of biological membranes: phospholipids, glycolipids and cholesterol • The organization dual layer or in micelle • The fluidity of the phospholipid bilayer: the role of unsaturated fats • The intrinsic and extrinsic proteins • The theory of fluid Mosaic The transport mechanisms through membranes • the lipid bilayer permeability • simple and facilitated diffusion • Passive transport and active transport • channel proteins and carrier proteins: kinetic properties of the two systems and operating methods • Glucose Transporter • Pump Na + / K + ATPase: operating mode • Antiport and Simport • ligand-activated channels and channels activated by voltage • The membrane potential and the action potential Mitochondria and ATP synthesis • General knowledge on the process of glycolysis, oxidation of pyruvate, Krebs cycle and the electron transport chain • The structure of the mitochondrion • The mitochondrial genome • The evolutionary origin of mitochondria • The transport of proteins in mitochondria The endoplasmic reticulum and Golgi apparatus • Summary of the membrane proteins and secretion • Glycosylation and maturation of proteins in the Golgi • vesicular traffic: vesicle formation, vesicle movement, recognition of the target organelle • Exocytosis Addressing mechanisms of proteins in cellular compartments • signal sequences and protein localization (entry into the nucleus, the endoplasmic reticulum, the mitochondria) Endocytosis • pinocytosis, phagocytosis and receptor-mediated endocytosis Degradation of biological macromolecules in the cell • The lysosome • The proteasome • The peroxisome The cytoskeleton • Microfilaments contractile actin and myosin and cell movement • intermediate filaments • Microtubules and organelle movement • Muscle contraction The extracellular matrix • The collagen, laminin the fibronectin, proteoglycans • The extracellular matrix proteins establish multiple interactions • The matrix-cell interaction is mediated by specific receptors: the integrins • Structure and function of integrins and cadherins The cell cycle and mitosis • The cell cycle G1, S, G2, and M. • The role of cyclins and cdk in cell cycle progression Cell death and apoptosis • necrosis • Apoptosis: the intrinsic and extrinsic pathways The flow of genetic information: the definition of gene the ENCODE era • DNA replication • DNA Transcription: synthesis of RNA and their maturation • The genetic code and the translation of proteins
Expected learning objectivesD1 - KNOWLEDGE AND UNDERSTANDING ABILITY. At the end of the course, the student must demonstrate adequate knowledge of: differences between prokaryotic and eukaryotic cells; connection between chemical composition and function of biological macromolecules; cell structures of animal cells, function of organelles and components and functions of endomembrane and cytoskeletal systems; the Central Dogma and the relationship between genes and proteins; DNA replication processes, transcription and translation in eukaryotes D2 - CAPACITY TO APPLY KNOWLEDGE AND UNDERSTANDING. The student will have to show the ability to use acquired knowledge and concepts to reason critically, showing the ability to make connections between different topics and to apply the acquired knowledge to analyze biology experiments. D3 - JUDGMENT AUTONOMY. The student must demonstrate that he / she is able to critically examine the information acquired and to be able to independently discuss applications and application problems.Furthermore, he/she must be able to constructively judge the teaching provided. D4 - COMMUNICATION SKILLS. The student will have to demonstrate possession of the ability to communicate the knowledge acquired to their colleagues and teachers using the terminology of cell biology. D5- LEARNING SKILLS. The student must show possession of the learning ability useful for the continuous updating of knowledge in this discipline.
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CourseHistology and developmental biology
Course IDMS0732
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPRAT Maria Giovanna
TeachersFOLLENZI Antonia, PRAT Maria Giovanna
CFU6
Teaching duration (hours)48
Individual study time 102
SSDBIO/17 - ISTOLOGIA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year1
PeriodSecondo Semestre
Sites and/or partitions
Gruppo B
Gruppo A
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CourseHistology and developmental biology
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderFOLLENZI Antonia
TeachersFOLLENZI Antonia
Course typeGruppi
Year1
PartitionGruppo B
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CourseHistology and developmental biology
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPRAT Maria Giovanna
TeachersPRAT Maria Giovanna
Course typeGruppi
Year1
PartitionGruppo A
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CourseHuman Anatomy
Course IDBT015
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBOCCAFOSCHI Francesca
TeachersBOCCAFOSCHI Francesca
CFU4
Teaching duration (hours)32
Individual study time 68
SSDBIO/16 - ANATOMIA UMANA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year1
PeriodSecondo Semestre
Sites and/or partitions
Gruppo B
Gruppo A
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CourseHuman Anatomy
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBOCCAFOSCHI Francesca
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractAnatomical terminology, anatomical position, position and movement definition, organs, apparatus and systems. Locomotory apparatus. Generality, structure of bone elements; Type, structure and functional meanings of joints; Structure of striated muscle and smooth muscle, descriptive and functional anatomy of muscle complexes. Cardiovascular system. The heart: position and relationships. Veins and arteries, topographical anatomy of the arterial and venous network and vascularization areas. Respiratory system. Descriptive and functional anatomy of the upper and lower airways (nasal cavities, larynx, trachea and bronchi, pulmonary parenchyma, pleura). Digestive system. Descriptive and functional anatomy of: Buccal Cavity, Faringe, Esophagus, Stomach, Intestine and Glands attached to the Digestive System (Liver, Pancreas, Milza). Urinary tract. Descriptive and functional anatomy of: Kidneys, Renal Pelvis, Uretheres, Bladder, Male and Female Uretra. Genital apparatus. MALE - Descriptive and Functional Anatomy of Testicles, Prostate and Penis. FEMALE - Descriptive and Functional Anatomy of Ovary, Uterus, Vagina. Endocrine system. Descriptive and functional anatomy of: Hypophysis, Thyroid, Parathyroid, Endocrine Pancreas, Surrenal Glands and Gonads. Nervous system. CENTRAL NERVOUS SYSTEM - Descriptive and Functional Anatomy of Spinal Cord, Encephalic Corpus, Cervelletto, Diencefalo, Telencephalo and Core Nuclei. PERIPHERAL NERVOUS SYSTEM - Functional correlations of the sympathetic and parasympathetic system, nomenclature and meaning of the Cranial Nerves. Eye and Ear functional anatomy.
Reference textsAnatomia Umana - Saladin - Casa Editrice Piccin
Teaching targetsThe module provides the students with a theoretical knowledge of the microscopic and macroscopic Human Anatomy with particular reference to the most important anatomical districts for clinical practice and human pathology.
Didattics MethodsPresentations in MS-Power Point format, educational websites. There are interactive exercises for self-assessment of learning during the course.
Grading rulesWritten exam - multiple choice answers
Expected learning objectivesAt the end of the module the student will possess knowledge of the micro and macroscopic organization of the organs, apparatus and systems of the human body, understanding the relationship between structure and function. The students must to communicate their knowledge using correct scientific and anatomical terminology.
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CourseHuman Anatomy
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBOCCAFOSCHI Francesca
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractAnatomical terminology, anatomical position, position and movement definition, organs, apparatus and systems. Locomotory apparatus. Generality, structure of bone elements; Type, structure and functional meanings of joints; Structure of striated muscle and smooth muscle, descriptive and functional anatomy of muscle complexes. Cardiovascular system. The heart: position and relationships. Veins and arteries, topographical anatomy of the arterial and venous network and vascularization areas. Respiratory system. Descriptive and functional anatomy of the upper and lower airways (nasal cavities, larynx, trachea and bronchi, pulmonary parenchyma, pleura). Digestive system. Descriptive and functional anatomy of: Buccal Cavity, Faringe, Esophagus, Stomach, Intestine and Glands attached to the Digestive System (Liver, Pancreas, Milza). Urinary tract. Descriptive and functional anatomy of: Kidneys, Renal Pelvis, Uretheres, Bladder, Male and Female Uretra. Genital apparatus. MALE - Descriptive and Functional Anatomy of Testicles, Prostate and Penis. FEMALE - Descriptive and Functional Anatomy of Ovary, Uterus, Vagina. Endocrine system. Descriptive and functional anatomy of: Hypophysis, Thyroid, Parathyroid, Endocrine Pancreas, Surrenal Glands and Gonads. Nervous system. CENTRAL NERVOUS SYSTEM - Descriptive and Functional Anatomy of Spinal Cord, Encephalic Corpus, Cervelletto, Diencefalo, Telencephalo and Core Nuclei. PERIPHERAL NERVOUS SYSTEM - Functional correlations of the sympathetic and parasympathetic system, nomenclature and meaning of the Cranial Nerves. Eye and Ear functional anatomy.
Reference textsAnatomia Umana - Saladin - Casa Editrice Piccin
Teaching targetsThe module provides the students with a theoretical knowledge of the microscopic and macroscopic Human Anatomy with particular reference to the most important anatomical districts for clinical practice and human pathology.
Didattics MethodsPresentations in MS-Power Point format, educational websites. There are interactive exercises for self-assessment of learning during the course.
Grading rulesWritten exam - multiple choice answers
Expected learning objectivesAt the end of the module the student will possess knowledge of the micro and macroscopic organization of the organs, apparatus and systems of the human body, understanding the relationship between structure and function. The students must to communicate their knowledge using correct scientific and anatomical terminology.
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CourseInorganic chemistry
Course IDMS0725
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBOCCALERI Enrico
TeachersBOCCALERI Enrico, GABANO Elisabetta, MINASSI Alberto, CAPRIOGLIO Diego
CFU9
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year1
PeriodPrimo Semestre
Grading typeFinal grade
Modules
Course ID Course SSD Teachers
MS0726 General chemistry CHIM/03 - CHIMICA GENERALE E INORGANICA BOCCALERI Enrico, GABANO Elisabetta
MS0727 Introductory chemistry laboratory CHIM/06 - CHIMICA ORGANICA MINASSI Alberto, CAPRIOGLIO Diego
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CourseGeneral chemistry
Course IDMS0726
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBOCCALERI Enrico
TeachersBOCCALERI Enrico, GABANO Elisabetta
CFU6
Teaching duration (hours)48
Individual study time 102
SSDCHIM/03 - CHIMICA GENERALE E INORGANICA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year1
PeriodPrimo Semestre
Sites and/or partitions
Gruppo A
Gruppo B
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CourseGeneral chemistry
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBOCCALERI Enrico
TeachersBOCCALERI Enrico
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractThe purpose of the course is to provide students the basic knowledge in general and inorganic chemistry. The connection with the various biological disciplines will be discussed, in particular with the use of examples and numerical exercises
Reference textsThe presentations will be available on the D.I.R. site. Suggested Reference Books: -Kotz, Treichel, Townsend, “Chimica” Edises; -Schiavello, Palmisano “Elementi di Chimica” Edises; -Masterton, Hurley: "Chimica: Principi e Reazioni", Piccin; -Tro: "Chimica: un approccio molecolare", Edises; -Whitten, Davis, Peck, Stanley, Chimica (9 Ed.), Piccin. Stoichiometry Reference Books: -Breschi, Massagli: "Stechiometria", Edizioni ETS; -Michelin-Lausarot, Vaglio: "Fondamenti di stechiometria", Piccin; -Uguzzoli: "Come risolvere i problemi di chimica", Casa Editrice Ambrosiana
Teaching targetsThe course aims at introducing the students of the main understanding of chemical phenomena, with details of applicative aspects. A supplementary noteworthy effort will be set on the chemical behavior of aqueous solutions and related chemical reactions, to establish the necessary basis for supplementary chemistry courses and related biological courses.
PrerequisitesNone
Didattics MethodsClassroom lessons in which the main topics reported in the abovementioned program will be disseminated. Course is hereafter completed by practical classroom exercises to tackle a few of the exposed topics.
Other informationsLearning Control: Interactive discussion of the explained topics will be stimulated. Students will be involved in the shared resolution of applicative exercises, to strengthen their knowledge and test actual progresses.
Grading rulesThe final exam is a 2-hour written test, containing questions of both general chemistry (6 questions, each with a maximum score of 2 points) and applied stoichiometry exercises (6 numerical exercises, each one with a maximum score of 3 points). The sum of the points must be at least 18 to pass the examination.
Full argumentsThe matter: physical states, definition of matter (elements, compounds and mixtures). The atomic structure. Atoms and isotopes. Compounds and molecular representations (the laws of definite proportions and of multiple proportions). Atomic and molecular weights. Definition of a.m.u. Avogadro's Number and the concept of mole. Chemical reactions (oxidation numbers, balance of redox and non-redox reactions). The quanto-mechanical description of the atom (orbital concept, quantum numbers, the Aufbau principle). The Periodic Table and its correspondence with the electronic structure of elements. Periodic properties. The chemical bond: the octet rule, ionic bonds, covalent bonds in the theory of valence bond. Hybrid orbitals. The concept of resonance. Bond order and bond lengths, multiple bonds. Electronegativity, polarity of bonds and molecules. Intermolecular forces (ion-dipole, dipole-dipole, hydrogen bonds, induced dipoles, dispersion forces). The aggregation states of matter. Gas: definition and the law of perfect gases. Liquid and solid states. State changes (state diagrams). Solutions: concentrations and colligative properties. Thermodynamics: definitions, the principles of thermodynamics, enthalpy, entropy, free energy. Chemical kinetics: definitions and integrated kinetic laws (zero order, 1st and 2nd order), activation energy and the control of a reaction rate (Arrhenius' law), catalysts. Chemical reactions: the law of the action of masses and Le Chatelier principle, influence of experimental parameters on the chemical constants. Reactions in aqueous solutions. Autoprotonation of water and the pH, acid-base concepts, strong and weak acids and bases (classification and their strength, the structure of most important acids containing N, P, S and halogen elements), hydrolysis, buffer solutions. Products of solubility: definition and applications. Electrochemistry: standard potentials and Nernst's equation, galvanic and electrolitic cells, corrosion. Descriptive Chemistry: metals, semimetals and non-metals. Principal properties of these groups. Stoichiometry exercises related to these issues.
Expected learning objectivesKnowledge of the fundamental aspects of the matter at the elementary and compositional level, of the chemical laws underlying the formation, geometry, stability and reactivity of compounds. Knowledge of the aspects that correlate the structure at the atomic and molecular level with the physical states and its transformations. Ability to describe and determine the fundamental features of the chemistry in aqueous solution (concentration, colligative properties, pH). Knowledge of the main aspects of the equilibrium and of the thermodynamic variables related to the energetic aspects and to the spontaneity of the reactions, and of the rate of the chemical reactions. Capability to apply stoichiometric calculations on ponderal ratios in reactions, on pH determination for strong and weak acid and basic solutions, salt hydrolysis and buffer solutions
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CourseGeneral chemistry
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGABANO Elisabetta
TeachersGABANO Elisabetta
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageItalian
AbstractThe purpose of the course is to provide students the basic knowledge in general and inorganic chemistry. The connection with the various biological disciplines will be discussed, in particular with the use of examples and numerical exercises.
Reference textsThe presentations will be available on the D.I.R. site. Suggested Reference Books: -Kotz, Treichel, Townsend, “Chimica” Edises; -Petrucci et al.: “Chimica generale. Principi ed applicazioni moderne”, Piccin; -Masterton, Hurley: "Chimica: Principi e Reazioni", Piccin; -Tro: "Chimica: un approccio molecolare", Edises; -Whitten, Davis, Peck, Stanley, Chimica (9 Ed.), Piccin; -Speranza et al., Chimica generale e inorganica, Edi.Ermes. Stoichiometry Reference Books: -Breschi, Massagli: "Stechiometria", Edizioni ETS; -Michelin-Lausarot, Vaglio: "Fondamenti di stechiometria", Piccin; -Uguzzoli: "Come risolvere i problemi di chimica", Casa Editrice Ambrosiana
Teaching targetsThe course aims at introducing the students of the main understanding of chemical phenomena, with details of applicative aspects. A supplementary noteworthy effort will be set on the chemical behavior of aqueous solutions and related chemical reactions, to establish the necessary basis for supplementary chemistry courses and related biological courses.
PrerequisitesBasic (high school) knowledge of mathematics and physics
Didattics MethodsClassroom lessons in which the main topics reported in the abovementioned program will be disseminated. Course is hereafter completed by practical classroom exercises to tackle a few of the exposed topics.
Other informationsLearning Control: Interactive discussion of the explained topics will be stimulated. Students will be involved in the shared resolution of applicative exercises, to strengthen their knowledge and test actual progresses.
Grading rulesThe exam of the whole course consists of a 2-hour written exam that evaluates the student's ability to apply the knowledge acquired during the course. This test will consist of 6 exercises and 6 multiple choice questions. The exercises may include: the balance of chemical reactions, property of the solutions, acid-base properties, Lewis structure. The questions may include: the electronic structure of the atoms, the periodic system, the chemical bond, the intermolecular forces and the physical states of matter, thermodynamics and kinetics, acids and bases, solubility, electrochemistry. The exercises/questions will be chosen so that the entire program is covered, and the student can demonstrate that they know and understand at least the basic concepts through their applications. The difficulty level of the exercises corresponds to the program and the reference texts indicated. Each of the six exercises will be awarded max 3 points, while each of the six questions will be awarded max 2 points (if error-free). The written test will be passed if the sum of the scores is not less than 18 points (18/30).
Full argumentsThe matter: physical states, definition of matter (elements, compounds and mixtures). The atomic structure. Atoms and isotopes. Compounds and molecular representations (the laws of definite proportions and of multiple proportions). Atomic and molecular weights. Definition of a.m.u. Avogadro's Number and the concept of mole. Chemical reactions (oxidation numbers, balance of redox and non-redox reactions). The quanto-mechanical description of the atom (orbital concept, quantum numbers, the Aufbau principle). The Periodic Table and its correspondence with the electronic structure of elements. Periodic properties. The chemical bond: the octet rule, ionic bonds, covalent bonds in the theory of valence bond. Hybrid orbitals. The concept of resonance. Bond order and bond lengths, multiple bonds. Electronegativity, polarity of bonds and molecules. Intermolecular forces (ion-dipole, dipole-dipole, hydrogen bonds, induced dipoles, dispersion forces). The aggregation states of matter. Gas: definition and the law of perfect gases. Liquid and solid states. State changes (state diagrams). Solutions: concentrations and colligative properties. Thermodynamics: definitions, the principles of thermodynamics, enthalpy, entropy, free energy. Chemical kinetics: definitions and integrated kinetic laws (zero order, 1st and 2nd order), activation energy and the control of a reaction rate (Arrhenius' law), catalysts. Chemical reactions: the law of the action of masses and Le Chatelier principle, influence of experimental parameters on the chemical constants. Reactions in aqueous solutions, autoprotonation of water and the pH, acid-base concepts, strong and weak acids and bases (classification and their strength, the structure of most important acids containing N, P, S and halogen elements), hydrolysis, buffer solutions. Products of solubility: definition and applications. Electrochemistry: standard potentials and Nernst's equation, galvanic and electrolitic cells, corrosion. Descriptive Chemistry: metals, semimetals and non-metals. Principal properties of these groups. Stoichiometry exercises related to these issues.
Expected learning objectivesKnowledge and understanding: theoretical and operational knowledge about the fundamental laws of chemistry (mol, reaction, bonds and molecular structure, states of matter, solutions, equilibrium, solution pH, thermodynamics, kinetics, electrochemistry); acquisition of a vocabulary of chemical terms to be able to expose chemical-scientific topics in a precise, concise and clear manner. Applying knowledge and understanding: ability to apply the theory to assign of the names of the most common inorganic chemical compounds, to balance a chemical reaction, to perform stoichiometric calculations, to solve numerical problems with gases, solutions, and acid or basic substances, and electrochemistry; ability to correlate the chemical structure with the physical properties and reactivity of the compounds. Making judgements: ability to interpret and rationalize chemical reactions from a critical and non-mnemonic point of view, using a scientific methodological approach to be applied to subsequent studies in other fields of chemistry and biology. Communication skills: ability to use a suitable scientific language during the learning control in the classroom.
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CourseIntroductory chemistry laboratory
Course IDMS0727
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMINASSI Alberto
TeachersMINASSI Alberto, CAPRIOGLIO Diego
CFU3
Teaching duration (hours)16
Individual study time 47
SSDCHIM/06 - CHIMICA ORGANICA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year1
PeriodPrimo Semestre
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseIntroductory chemistry laboratory
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersMINASSI Alberto
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
Abstract• Molarity • Normality • Molality •% w / w, w / v, v / v • pH and factors that influence the pH • Buffer solution • Quantitative analysis exercises • Volumetric analysis (titration)
Reference textsFondamenti di Stechiometria Giomini, Balestrieri, Giustino, edises
Teaching targetsthe course has the objective to provide the basic knowledge for calculating the concentrations of the solutions in the various forms that are commonly used in the laboratory whether chemical and pharmacological. At the end of the course students should be able to apply theoretical formulas to practical problems of dilution and preparation of solutions of known titre.
Prerequisitesbases of general chemistry
Didattics Methodsclassroom lectures with computer support: projection of powerpoint slides and videos. The slides are present on the site in advance of the start of the courses. brief presentation of the practical part in order to introduce students to what will be the experiences that they will be addressed in the laboratory and what are the main instruments that they will use exercises to test the learning means inconceivable of the basic formulas for the calculation of the concentrations of solutions In the didactic laboratories the students will do experiments to test their ability of the application of the concepts acquired during the first part of the course. every experience has a dedicated tutor who will follow and support the students in the performance of their work
Grading ruleswritten examination with problems on the preparation of standard solutions of various salts and inorganic compounds and the determination of the concentration of solutions of unknown titre
Full argumentsintroduction to solutions, suspensions, emulsions, and their differences. Introduction to the various chemical and physical units for the calculation of concentrations of solutions. exercises done in the classroom for learning evaluation. volumetric methods: theoretical and practical concepts of the use of titrations.
Expected learning objectivesthe student is able to apply the concepts learned to the daily laboratory practice
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CourseIntroductory chemistry laboratory
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCAPRIOGLIO Diego
TeachersCAPRIOGLIO Diego
Course typeGruppi
Year1
PartitionGruppo B
Teaching languageitalian
AbstractMolarity, Normality, Molality,% p / p, p / v, v / v, pH and factors affecting pH, acidity and constant acid dissocation, buffer solution, quantitative analysis, volumetric analysis (titrations)
Reference textsno specific text.
Teaching targetsThe objective of the theoretical lessons is the knowledge and the method of using the basic glassware used in chemical laboratories, stoichiometric calculations and well-known preparation preparations, volumetric analysis (titration) principles and laboratory safety. Laboratory practice: putting into practice the knowledge gained during the theoretical lessons.
Prerequisitesnone
Didattics MethodsClassroom lessons. Copy of the transparencies used during the course lessons available on the site. Exercises in the classroom. laboratory exercises
Grading ruleswritten exam where the ability to calculate the properties of solutions.
Full argumentsCFU 1 • Molarity • Normality • Molality •% p / p, p / v, v / v • pH and factors affecting pH • Buffer solution • Quantitative analysis • Volumetric analysis (titrations) CFU 2 Basic equipment in a chemical laboratory, glassware and its use, plastics and its use, scales, stoichiometric calculations, prediction of ionization, concentration, pH and related calculations. Safety and behavioral rules in the laboratory. CFU 3 Practical exercises in the lab
Expected learning objectivesthe student at the end of the course must have learned the main analytical laboratory techniques. In addition to this he must have learned how to calculate the main properties of the solutions.
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CourseOrganic Chemistry
Course IDMS0733
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderAPPENDINO Giovanni Battista
TeachersNEGRI Roberto, APPENDINO Giovanni Battista, CAPRIOGLIO Diego
CFU6
Teaching duration (hours)48
Individual study time 102
SSDCHIM/06 - CHIMICA ORGANICA
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryA - Base
Year1
PeriodSecondo Semestre
Grading typeFinal grade
Sites and/or partitions
Gruppo B
Gruppo A
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CourseOrganic Chemistry
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderNEGRI Roberto
TeachersNEGRI Roberto
Course typeGruppi
Year1
PartitionGruppo B
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CourseOrganic Chemistry
Academic Year2018/2019
Year of rule2018/2019
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderAPPENDINO Giovanni Battista
TeachersAPPENDINO Giovanni Battista, CAPRIOGLIO Diego
Course typeGruppi
Year1
PartitionGruppo A
Teaching languageItalian
AbstractThe course aims at providing students the basic knowledge of organic chemistry necessary to the study of the biological mechanisms involved in cell metabolism and its regulation. It involves a structural section (nomenclature, functional groups, stereochemistry, acid-base and redox properties) and a mechanistic section where the main mechanisms involved in reactivity are systematically addressed, exemplifying them with the description of the main classes of organic compounds
Reference textsBotta Chimica Organica (second edition) Edi-Ermes W.H. Brown – T Poon, Introduzione alla Chimica Organica; EdiSES McMurry – Fondamenti di chimica organica – Zanichelli. For the exercises: D’Auria, Taglialatela-Scafati, Zampella: Guida ragionata allo svolgimento di esercizi di chimica organica (seconda edizione), Loghia The slides used in the course are available on the DIR platform.
Teaching targetsa) To recognize functional groups and predict topological and reactivity properties of organic compounds. b) To master polar reactivity mechanisms: (aliphatic and aromatic nucleophilic substitution, acyl nucleophilic substitution, aromatic electrophilic substitution, electrophilic addition, nucleophilic addition, elimination) and the basic concepts of radical reactivity.
PrerequisitesBasic knowledge of general chemistry
Didattics MethodsThe lessons will use both slide projection (available in Powerpoint format on line via DIR) and writing on the blackboard.
Other informationsExercises sections preparatory to the exam will be held on regular basis to complement the mainstream lessons. Particular emphasis will be given to multiple-choice questions.
Grading rulesThe exam is based on a 20-multiple-choice question test carried out with a computer.
Full argumentsCredit 1: Structure and properties of organic compounds 1. General introduction to the chemical bond 2. Covalent, ionic and covalent polar bonds: properties and geometry. 3. Legame chimico covalente, ionico e covalente polare: proprietà e geometria-2 4. Formule di struttura di Lewis e cariche formali e formule di risonanza 5. Aromaticità 6. Esercizi di ricapitolazione-1 7. Esercizi di ricapitolazione-2 8. Essercizi di ricapitolazione-3 9. Credito 2: Gruppi funzionali, proprietà fisiche e nomenclatura 10. Gruppi funzionali e loro influenza sulle proprietà fisiche e chimiche (acidità e basicità) 11. Interazioni inter- and intra molecolari e loro effetto su proprietà e reattività 12. Nomenclatura dei composti organici 13. Esercitazioni di ricapitolazione-1 14. Esercitazioni di ricapitolazione-2 15. Esercitazioni di ricapitolazione-3 16. Esercitazioni di ricapitolazione-4 17. Esercitazioni di ricapitolazione-5 18. Credito 3: Isomeria, analisi conformazione e stereochimica 19. Isomeria: definizione e classificazione 20. Analisi conformazionale dei composti aciclici e ciclici. Analisi configurazionale: alcheni 21. Chiralità e analisi configurazionale:diastereomeria e enantiomeria-1 22. Chiralità e analisi configurazionale:diastereomeria e enantiomeria-2 23. Esercizi di ricapitolazione-1 24. Esercizi di ricapitolazione-2 25. Esercizi di ricapitolazione-3 26. Esercizi di ricapitolazione-4 27. Credito 4: Gruppi funzionali e loro reattività-1 28. Reattività di alcani, alcheni e alchinii: Addizione elettrofila e idrogenazione catalitica 29. Alogenuri alchilici e reazioni di sostituzione nucleofila (SN) mono-e bimolecolare 30. Reazione di eliminazione mono-e bimolecoalre. Competizione con SN 31. Alcoli e loro reattività 32. Esercizi di ricapitolazione-1 33. Esercizi di ricapitolazione-2 34. Esercizi di ricapitolazione-3 35. Esercizi di ricapitolazione-4 36. Credito 5: Gruppi funzionali e loro reattività-2 37. Eteri, epossidi, tioli e tioeteri 38. Ammine 39. Derivati carbonilici e reazione di addizione nucleofila 40. Tautomeria e reazioni di condensazione-1 41. Acidi carbossilici e reazioni di sostituzione nucleofila acilica-1 42. Derivati degli acidi carbossilici 43. Esercizi di ricapitolazione-1 44. Esercizi di ricapitolazione-2 45. Credito 6: Gruppi funzionali-e loro reattività-3/biomolecoler 46. Benzene: aromaticità, nomenclatura e proprietà 47. Reattività del benzene e reazioni di sostituzione elettrofila aromatica 48. Fenoli e ammine aromatiche. Sostituzione nucleofila aromatica 49. Reazioni radicaliche 50. Amminoacidi e peptidi-1 51. Amminoacidi e peptidi-2 52. Zuccheri 53. Lipidi
Expected learning objectivesStructural, mechanistic and reactivity bases necessary to study biomolecules and cellular metabolism
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CourseBasics of immunology and medical microbiology
Course IDMS0744
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderAZZIMONTI Barbara
TeachersDIANZANI Umberto, CHIOCCHETTI Annalisa, AZZIMONTI Barbara
CFU10
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year2
PeriodSecondo Semestre
Grading typeFinal grade
Teaching languageIl corso è tenuto in lingua italiana. Le slides in formato power point possono essere sia in lingua italiana che inglese.
AbstractCoerentemente con gli obiettivi formativi specifici del CdS, riportati nella SUA-CdS, quadro A4.a, i contenuti sono di seguito descritti: La cellula batterica: organizzazione e struttura. Divisione e crescita. Il metabolismo batterico. Flora microbica residente: il microbiota. Principi di virulenza batterica Meccanismi di trasferimento genico Nozioni di base sui principali batteri patogeni I virus: organizzazione e struttura, coltivazione e moltiplicazione, patogenicità. Nozioni di base sui principali virus patogeni Nozioni di base sugli agenti subvirali: i prioni. Nozioni di base sui microrganismi eucarioti: i protisti . Controllo della crescita microbica I vaccini La risposta immune contro le infezioni Esercitazioni teorico pratiche -Cellule e organi del sistema immunitario. -Antigeni-Anticorpi. -Complemento. -Anticorpi monoclonali e tecniche di laboratorio. -TCR e Molecole MHC. -Cellule presentanti l’antigene. -Maturazione dei linfociti B e T. -Citochine. -Funzioni dei linfociti T e NK. -Memoria immunologica. -Immunoelusione. -Immunizzazione passiva e attiva. -Ipersensibilità. -Malattie autoimmuni.
Reference textsTesti consigliati: - Principi di Microbiologia medica. Casa Editrice Ambrosiana. Antonelli, Clementi, Pozzi, Rossolini, III edizione - Microbiologia medica. Piccin, Carrol, Morse, Mietzner, Miller, ventisettesima edizione - Coico, Immunologia, un corso breve. Edi Hermes- - Murphy, Immunobiologia di Janeway. Piccin
Teaching targetsAl termine del corso gli studenti devono essere in grado di raggiungere: Obiettivi formativi specifici misurabili e raggiungibili nel periodo di tempo previsto; la microbiologia è una disciplina in continuo sviluppo, una scienza in cui convergono altre discipline come la biologia molecolare, la chimica e biochimica, la genetica, ecc. Il corso ha lo scopo di fornire agli studenti la possibilità di fare collegamenti fra le discipline ed elementi sufficienti per comprendere la biologia dei principali microrganismi e gli habitat microbici. L’insegnamento si propone di formare gli studenti sui diversi aspetti della Microbiologia generale e medica, con particolare riferimento alla organizzazione strutturale e molecolare e alle funzioni degli organismi procariotici, eucariotici e dei virus e i rispettivi meccanismi patogenetici. Lo studente acquisirà conoscenze teoriche sui caratteri generali e speciali dei microrganismi procarioti (batteri), eucarioti (miceti e protozoi) e virus. Il corso fornisce agli studenti le conoscenze approfondite del mondo dei microrganismi in modo tale da comprendere le interazioni positive e dannose tra microrganismo e uomo. Il corso fornisce, inoltre, conoscenze teorico-pratiche delle metodologie di base del laboratorio di microbiologia tali da consentire allo studente gli elementi per poter scegliere di utilizzare e applicare in modo autonomo i metodi appropriati di analisi per la diagnostica delle patologie infettive. l corso presenta un approfondimento sui temi relativi ai rapporti tra il corpo umano e microrganismi, utilizzando un’ottica medica. Verranno analizzate le infezioni causate da alcuni importanti patogeni umani, con particolare riferimento ai meccanismi molecolari implicati. Infine verranno presentate le principali tecniche diagnostiche impiegate per l’identificazione dei più importanti agenti patogeni. Gli studenti dovranno altresì acquisire conoscenze nell’ambito del funzionamento del sistema immunitario a livello di immunità naturale e acquisita (adattativa) atte a comprendere anche i meccanismi di immunoelusione, le pratiche di vaccinazione e l’eziologia e patogenesi delle malattie allergiche e autoimmuni.
Prerequisitesper la comprensione del corso gli studenti iscritti devono essere in possesso di un corredo minimo dei principi fondamentali di biologia cellulare, chimica, genetica, immunologia e microbiologia (saperi minimi). Devono altresì essere in possesso di conoscenze della lingua inglese di livello B1.
Didattics MethodsTutto il materiale didattico è caricato sul sito https://www.dir.uniupo.it/ I metodi didattici di cui si avvale il corso sono rappresentati da: - Presentazioni in formato MS-Power Point Per la disciplina Microbiologia verranno altresì - Proiettate Peer Reviewed Scientific Video Journals (JoVE) - Utilizzata la piattaforma di apprendimento "Kahoot"veloce e divertente utile alla verifica in itinere dell'apprendimento - Effettuate sercitazioni teorico-pratiche di laboratorio utili ad incentivare gli studenti nel loro ruolo di partecipatori attivi Il corso di Microbiologia, di 5 CFU complessivi (44 h) è organizzato nel seguente modo: - 32h di lezioni teoriche frontali, svolte in aula mediante l’utilizzo di diapositive in power-point, lavagna classica e piattaforma Kahoot, su tutti gli argomenti del corso. Tali lezioni includono una verifica diretta continua in aula del livello di comprensione dei vari argomenti trattati attraverso il dialogo, le domande e kahoot in modo da stimolare la motivazione, la curiosità, lo spirito critico, l'autonomia e il coinvolgimento nei processi di apprendimento degli studenti. - 12h di esercitazioni teorico-pratiche (di cui 6 sottoforma di visual experiments in aula e 6 in laboratorio didattico, queste ultime replicate in gruppi di 25 persone circa). Tali esercitazione consentono agli studenti di mettere in pratica e dunque applicare le principali tematiche e metodologie microbiologiche affrontate a lezione.
Other informationsEmail docente Coordinatore del Corso integrato: barbara.azzimonti@med.uniupo.it Il docente, al termine delle lezioni o previo contatto email, è disponibili a discutere eventuali dubbi inerenti le tematiche del corso. Il docente riceve gli studenti, previo appuntamento, nel suo studio c/o Palazzo Bellini, Dipartimento di Scienze della Salute, Via Solaroli 17, 28100 Novara. Al termine del corso verrà simulata la prova d'esame
Grading rulesData la numerosità degli studenti (gruppi A+B), la prova di valutazione finale consiste in un esame scritto di Microbiologia e orlae di Immunologia, nelle date prestabilite degli appelli, così composto: - Quindici domande a risposta multipla (più opzioni di risposta corretta); è necessario rispondere correttamente a tutte le opzioni per ottenere 1 punto/domanda (totale 15 punti); non vengono sottratti punti per opzioni di risposta non indicate correttamente - Due affermazioni da indicare come V/F (1.5 punti/afferamazione) e motivare in 4-5 righe (6 punti max/affermazione), per ottenere 7.5 punti/domanda (totale 15 punti). La somma dei due punteggi non deve essere inferiore a 18; il punteggio massimo raggiungibile è 30 e Lode. Le domande vertono su tutti gli argomenti svolti durante le lezioni in aula e le esercitazioni pratiche di laboratorio. L’obiettivo della prova d’esame consiste nel verificare il livello di raggiungimento degli obiettivi formativi precedentemente indicati e nella capacità di comunicare ed elaborare in modo critico per iscritto gli argomenti appresi in lingua italiana con appropriatezza di linguaggio (corretto uso di termini tecnico‐scientifici). L'esame serve inoltre a valutare l’acquisizione di conoscenze, la capacità di organizzare una risposta articolata con chiarezza espositiva, la sintesi e appropriatezza di linguaggio e l’approccio analitico/critico dello studente nei confronti delle nozioni apprese durante il corso. Sarà dedicato un momento alla correzione dei compiti scritti durante il quale ogni studente sarà messo nelle condizioni di riconoscere gli errori commessi ed individuare le risposte corrette
Full argumentsMicrobiologia medica La cellula batterica: organizzazione e struttura. Divisione e crescita. Il metabolismo batterico. Flora microbica residente: il microbiota. Principi di virulenza batterica: Le tossine batteriche: esotossine ed endotossine; la spora; la capsula; i pili ed i flagelli; gli enzimi; i siderofori; il biofilm batterico. Meccanismi di trasferimento genico: coniugazione, trasformazione, trasduzione, trasposizione. Nozioni di base sui principali batteri patogeni: Stafilococchi, Streptococchi, Enterobatteriaceae, Clostridi, Micobatteri, Neisserie, Pseudomonas, A. Baumannii, Pneumococchi, Clamidie ed Elicobatteri. I virus: organizzazione e struttura, coltivazione e moltiplicazione, patogenicità. Nozioni di base sui principali virus patogeni (tali temi verranno approfonditi durante la Laurea magistrale nel Corso di Virologia molecolare): Hepadnavirus ed altri virus causa di epatiti, Herpesvirus, Papillomavirus, Orthomyxovirus, Retrovirus. Nozioni di base sugli agenti subvirali: i prioni. Nozioni di base sui microrganismi eucarioti: i protisti . Controllo della crescita microbica: Disinfezione e sterilizzazione; gli antibiotici; antimicrobici naturali; i farmaci antivirali; meccanismi di resistenza (i superbatteri). I vaccini: anti-polio, -meningite, -difterite, -tetanica, -pertosse, -rosolia, -HPV, -epatite, -influenzale, -morbillo, -varicella. La risposta immune contro le infezioni. Aspecifica: infiammazione, fagociti, fagocitosi e killing, complemento; Specifica: umorale e cellulo-mediata. Evasione della risposta immune da parte dei microrganismi. Esercitazioni Teoriche (visual experiments) Diagnosi di laboratorio delle malattie infettive (tecniche microbiologiche): piastratura di batteri su terreni solidi (selettivi, differenziali, selettivi/differenziali); colorazione di Gram e Ziehl-Neelsen; Calcolo delle Unità formanti placca (CFU); saggi di vitalità batterica (XTT/MTT, Live/Dead assay, infrared assay); analisi del biofilm mediante Microscopia Elettronica a Scansione (SEM); immunofluorescenza e immunoistochimica; ibridazione in situ fluorescente (FISH); plaque assay; replica plate; l’antibiogramma, ELISA, PCR in microbiologia. Pratiche: piastratura, identificazione specie batteriche, colorazioni di Gram e Ziehl-Neelsen, antibiogramma, calcolo delle CFU e valutazione dell’attività metabolica batterica mediante saggio XTT. Immunologia -Generalità sul sistema immunitario, immunità aspecifica e specifica, naturale e acquisita (adattativa). -Cellule del sistema immunitario. Recettori e mediatori dell’immunità innata. -Organi del sistema immunitario. -Antigeni-Anticorpi. Struttura e funzione degli anticorpi e delle classi anticorpali. Caratteristiche degli antigeni riconosciuti dai linfociti B. Apteni. Struttura e trasduzione del segnale del BCR. -Il sistema del complemento. Via classica, alternativa, lectinica. Recettori e inibitori del complemento. -Anticorpi monoclonali e tecniche di laboratorio. Anticorpi monoclonali e policlonali. Immunofluorescenza, Western blot, RIA, ELISA, immunodiffusione. -TCR e Molecole MHC. Struttura di TCR e molecole MHC. Nomenclatura e genetica del sistema HLA. Presentazione dell’antigene (via endocitica, via citosolica, cross-presentazione, via lipidica). CD3 e corecettori CD4 e CD8, trasduzione del segnale per l’attivazione dei linfociti T. -Cellule presentanti l’antigene. Tipologia, funzione, secondo segnale. CD28, CTLA-4 and PD-1. -Generazione del repertorio recettoriale B. Riarrangiamento dei geni delle Ig. Maturazione antigene-indipendente e antigene-dipendente dei linfociti B. -Generazione del repertorio recettoriale T. Maturazione timica dei linfociti T effettori e dei linfociti T regolatori naturali. -Citochine. Generalità, struttura e funzione, recettori, trasduzione del segnale. -Attività helper, regolatorie e citotossiche cellulo-mediate. Th1, Th2, Th17, Treg naturali e indotti, CTL, NK, NKT, Tγδ. -Immunoelusione. Meccanismi di immunoelusione degli agenti infettivi e dei tumori. -Immunizzazione passiva e attiva. Vaccini tradizionali e di nuova generazione. -Ipersensibilità I, II, III, IV tipo. Eziologia, patogenesi, esempi. -Malattie autoimmuni. Patogenesi (mediate da anticorpi, cellulo-mediate). Eziologia (fattori scatenanti, fattori predisponenti).
Expected learning objectivesAPPRENDIMENTO, CONOSCENZA E COMPRENSIONE: Alla fine del corso, lo studente dovrebbe dimostrare di conoscere la struttura, substruttura e proprietà biologiche di batteri, virus e miceti e di aver compreso, nei tempi previsti dal corso, i principali meccanismi alla base dei processi infettivi causa di patologia umana. Dovrebbe inoltre dimostrare di conoscere le principali tecniche utilizzate in diagnostica batteriologica e virologica. CAPACITA' DI APPLICARE CONOSCENZA E COMPRENSIONE: Lo studente dovrebbe aver acquisito abilità in ambito della microbiologia medica tali da poterle applicare nella pratica di laboratorio. ABILITA' TRASVERSALI: Lo studente deve anche essere in grado di avere AUTONOMIA DI GIUDIZIO e ABILITA' di COMUNICARE e motivare in forma scritta e in pratica le sue scelte procedurali/sperimentali Lo studente dovrà inoltre aver appreso i fondamenti molecolari dell’immunologia anche in relazione alle applicazioni nel campo della diagnosticae della terapia e le basi cellulari e molecolari delle alterazioni della risposta immune ed infiammatoria.
Modules
Course ID Course SSD Teachers
BT029 Immunology MED/04 - PATOLOGIA GENERALE DIANZANI Umberto, CHIOCCHETTI Annalisa
MS0745 Medical microbiology with laboratory MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA AZZIMONTI Barbara
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CourseImmunology
Course IDBT029
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderDIANZANI Umberto
TeachersDIANZANI Umberto, CHIOCCHETTI Annalisa
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/04 - PATOLOGIA GENERALE
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodSecondo Semestre
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseImmunology
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersDIANZANI Umberto
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
Abstract-Cells and organs of the immune system. -Antigens-Antibodies. -Complement. -Monoclonal antibodies and laboratory technique. -TCR and MHC. -Antigen presenting cells. -B and T cell maturation. -Cytokines. -Functions of T and NK cells. -Immunological memory. -Immunoelusion. -Passive and active immunization. -Hypersensibility. -Autoimmune diseases.
Reference texts1) Coico, Immunologia, un corso breve. Edi Hermes- 2) Murphy, Immunobiologia di Janeway. Piccin
Teaching targetsTo acquire knowledges on the immune system function at the level of innate and adaptive immunity in order to understand also the mechanisms of immune evasion, vaccination, and the etiology and pathogenesis of allergic and autoimmune diseases.
PrerequisitesKnowledge of basic cell biology and genetics
Didattics MethodsFrontal teaching with slides
Other informationsnone
Grading rulesoral examination
Full arguments-Immune system. Innate and adaptive immunity. -Cells of the immune system. Receptors and mediators of innate immunity. -Organs of the immune system. -Antigens-Antibodies. Structure and function of the antibodies and antibody isotypes. Features of B cell antigens. Aptens. BCR structure and signaling. -Complement. Classic, alternative, lectinic pathway. Receptors and inhibitors. -Monoclonal antibodies and laboratory techniques. Monoclonal and polyclonal antibodies. Immunofluorescence, Western blot, RIA, ELISA, immunodiffusion. -TCR and MHC. Structure and function. The HLA system. Antigen presentation (endocytic, cytosolic, lipid pathways, cross-presentation). CD3, CD4, CD8, function and signaling. -Antigen presenting cells. Cell types, function second signal. CD28, CTLA-4 and PD-1. -B cell repertoire. Ig gene rearrangement. Antigen-independent and antigen-dependent maturation of B cells. -T cell repertoire. Thymic maturation of conventional and regulatory T cells. -Cytokines. Structure, function, receptors and signaling. -Helper and cytotoxic activities aof T cells. Th1, Th2, Th17, natural and induced Treg, CTL, NK, NKT, Tγδ. -Immune evasion. Mechanisms of immune evasion of infectious agents and tumors. -Passive and active immunization. Vaccines. -Type I, II, III, IV hypersensivity. Etiology, pathogenesis, examples. -Autoimmune diseases. Etiology, pathogenesis, examples.
Expected learning objectivesMolecular basis of immunology, also in relation to applications in the field of diagnostics and therapy. Cell type and molecules responsible for alterations of the immune response and inflammation.
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CourseImmunology
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCHIOCCHETTI Annalisa
TeachersCHIOCCHETTI Annalisa
Course typeGruppi
Year2
PartitionGruppo B
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CourseMedical microbiology with laboratory
Course IDMS0745
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderAZZIMONTI Barbara
TeachersAZZIMONTI Barbara
CFU5
Teaching duration (hours)32
Individual study time 81
SSDMED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodSecondo Semestre
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseMedical microbiology with laboratory
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderAZZIMONTI Barbara
TeachersAZZIMONTI Barbara
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageThe course is held in Italian. The power point slides can be in both Italian and English. The projected visual experiments are in English
AbstractConsistent with the specific training objectives of the CdS, reported in the SUA-CdS, framework A4.a, the contents are described below: The bacterial cell: organization and structure. Division and growth. Bacterial metabolism. Resident microbial flora: the microbiota. Principles of bacterial virulence: Bacterial toxins: exotoxins and endotoxins; the spore; the capsule; the pili and the scourges; enzymes; the siderophores; the bacterial biofilm. Mechanisms of gene transfer: conjugation, transformation, transduction, transposition. Basics of the main pathogenic bacteria: Staphylococci, Streptococci, Enterobacteriaceae, Clostridia, Mycobacteria, Neisseria, Pseudomonas, A. Baumannii, Pneumococci, Chlamydia and Helicobacteria. Viruses: organization and structure, cultivation and multiplication, pathogenicity. Basics of the main pathogenic viruses (these topics will be explored during the Master's Degree in the Course of Molecular Virology): Hepadnavirus and other viruses causing hepatitis, Herpesvirus, Papillomavirus, Orthomyxovirus, Retrovirus. Basics of subviral agents: prions. Basics on eukaryotic microorganisms: the protists. Control of microbial growth: Disinfection and sterilization; antibiotics; natural antimicrobials; antiviral drugs; resistance mechanisms (superbugs). Vaccines: anti-polio, -meningitis, -difteritis, -tetanica, -pertosse, -rosolia, -HPV, -hepatitis, -influenza, -morbillo, -varicella. The immune response against infections. Aspecifica: inflammation, phagocytes, phagocytosis and killing, complement; Specification: humoral and cell-mediated. Evasion of immune response by microorganisms. Exercises Theoretical (visual experiments) Laboratory diagnosis of infectious diseases (microbiological techniques): plating of bacteria on solid media (selective, differential, selective / differential); Gram and Ziehl-Neelsen staining; Calculation of plaque forming units (CFU); tests of bacterial viability (XTT / MTT, Live / Dead assay, infrared assay); Biofilm analysis using Scanning Electron Microscopy (SEM); immunofluorescence and immunohistochemistry; fluorescent in situ hybridization (FISH); plaque assay; replica plate; the antibiogram, ELISA, PCR in microbiology. Practices: plating, identification of bacterial species, Gram and Ziehl-Neelsen stains, antibiogram, calculation of CFU and evaluation of bacterial metabolic activity by XTT assay
Reference textsRecommended texts: - Principi di Microbiologia medica. Casa Editrice Ambrosiana. Antonelli, Clementi, Pozzi, Rossolini, III edizione - Microbiologia medica. Piccin, Carrol, Morse, Mietzner, Miller, ventisettesima edizione
Teaching targetsAt the end of the course the students must be able to reach: Specific training objectives that can be measured and reachable within the foreseen time frame; microbiology is a discipline in continuous development, a science in which other disciplines such as molecular biology, chemistry and biochemistry, genetics, etc. converge. The course aims to provide students with the opportunity to make connections between disciplines and elements sufficient to understand the biology of the main microorganisms and microbial habitats. The course aims to train students on the different aspects of general and medical microbiology, with particular reference to the structural and molecular organization and to the functions of prokaryotic, eukaryotic and virus organisms and their pathogenetic mechanisms. The student will acquire theoretical knowledge about the general and special characters of prokaryotic microorganisms (bacteria), eukaryotes (fungi and protozoa) and viruses. The course provides students with in-depth knowledge of the world of microorganisms so as to understand the positive and harmful interactions between microorganism and man. The course also provides theoretical and practical knowledge of the basic methodologies of the microbiological laboratory to allow the student the elements to choose to use and apply the appropriate methods of analysis for the diagnosis of infectious diseases. The course presents an in-depth study on the issues related to the relationship between the human body and microorganisms, using a medical perspective. Infections caused by some important human pathogens will be analyzed, with particular reference to the molecular mechanisms involved. Finally, the main diagnostic techniques used to identify the most important pathogens will be presented.
Prerequisitesfor the understanding of the course, students must be possess a minimum set of basic principles of cellular biology, chemistry, genetic, immunology and microbiology (minimal knowledge). They must also possess an English knowledge at level B1. Specific prerequisites are not foreseen.
Didattics Methodsfor the understanding of the course, students must be possess a minimum set of basic principles of cellular biology, chemistry, genetic, immunology and microbiology (minimal knowledge). They must also possess an English knowledge at level B1. Specific prerequisites are not foreseen. All the educational material is uploaded on the site https://www.dir.uniupo.it/ The teaching methods used by the course are represented by: - Presentations in MS-Power Point format - Screening of Peer Reviewed Scientific Video Journals (JoVE) - Use of the fast and fun "Kahoot" learning platform useful for ongoing assessment of learning - Laboratory theoretical-practical exercises to encourage students in their role as active participants The course of 5 total CFU (44 h) is organized as follows: - 32 hours of theoretical lectures, conducted in the classroom using power-point slides, classic blackboard and Kahoot platform, on all course topics. These lessons include a continuous direct examination in the classroom of the level of understanding of the various topics dealt with through dialogue, questions and kahoot in order to stimulate motivation, curiosity, critical spirit, autonomy and involvement in the learning processes of students. - 12 hours of theoretical-practical exercises (of which 6 in the form of visual experiments in the classroom and 6 in the didactic laboratory;these ones replicated in groups of about 25 people). These exercises allow students to put into practice and therefore apply the main microbiological issues and methodologies addressed in class. For practical exercises students are divided into groups of about 25 people.
Other informationsEmail: barbara.azzimonti@med.uniupo.it The teacher, at the end of the lessons or after contacting email, is available to discuss any doubts concerning the topics of the course. The teacher receives the students, by appointment, in his study c / o Palazzo Bellini, Department of Health Sciences, Via Solaroli 17, 28100 Novara. At the end of the course the exam will be simulated
Grading rulesSince the number of students is very high (groups A + B), the final evaluation test consists of a written exam, on the pre-established dates of the exams, as follows: - Fifteen multiple choice questions (more correct answer options; you must correctly answer to all the options to get 1 point/ question (total 15 points); points aren’t sottracted if answer opstions are not correctly marked - Two statements to be indicated as T / F (1.5 points) and motivated in 4-5 lines (6 points max), to obtain 7.5 points / question (total 15 points). The sum of the two scores must not be less than 18; the maximum achievable score is 30 cum laude. The questions focus on all the topics developed during the classroom lessons and practical laboratory exercises. The objective of the exam consists in verifying the level of achievement of the previously indicated educational objectives and in the ability to communicate and critically elaborate in writing the topics learned in italian language with appropriateness of language (correct use of technical-scientific terms). The examination also serves to evaluate the acquisition of knowledge, the ability to organize a structured response with clear clarity, the synthesis and appropriateness of language and the analytical / critical approach of the student towards the concepts learned during the course. A moment will be devoted to the correction of the written exams in which each student will be able to recognize its mistakes and identify the correct answers
Full argumentsSince the number of students is very high (groups A + B), the final evaluation test consists of a written exam, on the pre-established dates of the exams, as follows: - Fifteen multiple choice questions (more correct answer options; you must correctly answer to all the options to get 1 point/ question (total 15 points); points aren’t sottracted if answer opstions are not correctly marked - Two statements to be indicated as T / F (1.5 points) and motivated in 4-5 lines (6 points max), to obtain 7.5 points / question (total 15 points). The sum of the two scores must not be less than 18; the maximum achievable score is 30 cum laude. The questions focus on all the topics developed during the classroom lessons and practical laboratory exercises. The objective of the exam consists in verifying the level of achievement of the previously indicated educational objectives and in the ability to communicate and critically elaborate in writing the topics learned in italian language with appropriateness of language (correct use of technical-scientific terms). The examination also serves to evaluate the acquisition of knowledge, the ability to organize a structured response with clear clarity, the synthesis and appropriateness of language and the analytical / critical approach of the student towards the concepts learned during the course. A moment will be devoted to the correction of the written exams in which each student will be able to recognize its mistakes and identify the correct answers The bacterial cell: organization and structure. Division and growth. Bacterial metabolism. Resident microbial flora: the microbiota. Principles of bacterial virulence: Bacterial toxins: exotoxins and endotoxins; the spore; the capsule; the pili and the scourges; enzymes; the siderophores; the bacterial biofilm. Mechanisms of gene transfer: conjugation, transformation, transduction, transposition. Basics of the main pathogenic bacteria: Staphylococci, Streptococci, Enterobacteriaceae, Clostridia, Mycobacteria, Neisseria, Pseudomonas, A. Baumannii, Pneumococci, Chlamydia and Helicobacteria. Viruses: organization and structure, cultivation and multiplication, pathogenicity. Basics of the main pathogenic viruses (these topics will be explored during the Master's Degree in the Course of Molecular Virology): Hepadnavirus and other viruses causing hepatitis, Herpesvirus, Papillomavirus, Orthomyxovirus, Retrovirus. Basics of subviral agents: prions. Basics on eukaryotic microorganisms: the protists. Control of microbial growth: Disinfection and sterilization; antibiotics; natural antimicrobials; antiviral drugs; resistance mechanisms (superbugs). Vaccines: anti-polio, -meningitis, -difteritis, -tetanica, -pertosse, -rosolia, -HPV, -hepatitis, -influenza, -morbillo, -varicella. The immune response against infections. Aspecifica: inflammation, phagocytes, phagocytosis and killing, complement; Specification: humoral and cell-mediated. Evasion of immune response by microorganisms. Exercises Theoretical (visual experiments) Laboratory diagnosis of infectious diseases (microbiological techniques): plating of bacteria on solid media (selective, differential, selective / differential); Gram and Ziehl-Neelsen staining; Calculation of plaque forming units (CFU); tests of bacterial viability (XTT / MTT, Live / Dead assay, infrared assay); Biofilm analysis using Scanning Electron Microscopy (SEM); immunofluorescence and immunohistochemistry; fluorescent in situ hybridization (FISH); plaque assay; replica plate; the antibiogram, ELISA, PCR in microbiology. Practices: plating, identification of bacterial species, Gram and Ziehl-Neelsen stains, antibiogram, calculation of CFU and evaluation of bacterial metabolic activity by XTT.e assay
Expected learning objectivesLEARNING, KNOWLEDGE AND UNDERSTANDING: At the end of the course, the student should demonstrate knowledge of the structure, substructure and biological properties of bacteria, viruses and fungi and have understood, within the time frame, the main mechanisms underlying the infectious processes cause of human pathology. It should also demonstrate knowledge of the main techniques used in bacteriological and virological diagnostics. ABILITY TO APPLY KNOWLEDGE AND UNDERSTANDING: The student should have acquired skills in the field of medical microbiology that can be applied in laboratory practice. TRANSVERSAL SKILLS: The student must also be able to have JUDGMENT AUTONOMY and SKILLS to COMMUNICATE and motivate in writing and in practice his / her procedural / experimental choices.
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CourseMedical microbiology with laboratory
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderAZZIMONTI Barbara
TeachersAZZIMONTI Barbara
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageThe course is held in Italian. The power point slides can be in both Italian and English. The projected visual experiments are in English
AbstractConsistent with the specific training objectives of the CdS, reported in the SUA-CdS, framework A4.a, the contents are described below: The bacterial cell: organization and structure. Division and growth. Bacterial metabolism. Resident microbial flora: the microbiota. Principles of bacterial virulence: Bacterial toxins: exotoxins and endotoxins; the spore; the capsule; the pili and the scourges; enzymes; the siderophores; the bacterial biofilm. Mechanisms of gene transfer: conjugation, transformation, transduction, transposition. Basics of the main pathogenic bacteria: Staphylococci, Streptococci, Enterobacteriaceae, Clostridia, Mycobacteria, Neisseria, Pseudomonas, A. Baumannii, Pneumococci, Chlamydia and Helicobacteria. Viruses: organization and structure, cultivation and multiplication, pathogenicity. Basics of the main pathogenic viruses (these topics will be explored during the Master's Degree in the Course of Molecular Virology): Hepadnavirus and other viruses causing hepatitis, Herpesvirus, Papillomavirus, Orthomyxovirus, Retrovirus. Basics of subviral agents: prions. Basics on eukaryotic microorganisms: the protists. Control of microbial growth: Disinfection and sterilization; antibiotics; natural antimicrobials; antiviral drugs; resistance mechanisms (superbugs). Vaccines: anti-polio, -meningitis, -difteritis, -tetanica, -pertosse, -rosolia, -HPV, -hepatitis, -influenza, -morbillo, -varicella. The immune response against infections. Aspecifica: inflammation, phagocytes, phagocytosis and killing, complement; Specification: humoral and cell-mediated. Evasion of immune response by microorganisms. Exercises Theoretical (visual experiments) Laboratory diagnosis of infectious diseases (microbiological techniques): plating of bacteria on solid media (selective, differential, selective / differential); Gram and Ziehl-Neelsen staining; Calculation of plaque forming units (CFU); tests of bacterial viability (XTT / MTT, Live / Dead assay, infrared assay); Biofilm analysis using Scanning Electron Microscopy (SEM); immunofluorescence and immunohistochemistry; fluorescent in situ hybridization (FISH); plaque assay; replica plate; the antibiogram, ELISA, PCR in microbiology. Practices: plating, identification of bacterial species, Gram and Ziehl-Neelsen stains, antibiogram, calculation of CFU and evaluation of bacterial metabolic activity by XTT assay
Reference textsRecommended texts: - Principi di Microbiologia medica. Casa Editrice Ambrosiana. Antonelli, Clementi, Pozzi, Rossolini, III edizione - Microbiologia medica. Piccin, Carrol, Morse, Mietzner, Miller, ventisettesima edizione
Teaching targetsAt the end of the course the students must be able to reach: Specific training objectives that can be measured and reachable within the foreseen time frame; microbiology is a discipline in continuous development, a science in which other disciplines such as molecular biology, chemistry and biochemistry, genetics, etc. converge. The course aims to provide students with the opportunity to make connections between disciplines and elements sufficient to understand the biology of the main microorganisms and microbial habitats. The course aims to train students on the different aspects of general and medical microbiology, with particular reference to the structural and molecular organization and to the functions of prokaryotic, eukaryotic and virus organisms and their pathogenetic mechanisms. The student will acquire theoretical knowledge about the general and special characters of prokaryotic microorganisms (bacteria), eukaryotes (fungi and protozoa) and viruses. The course provides students with in-depth knowledge of the world of microorganisms so as to understand the positive and harmful interactions between microorganism and man. The course also provides theoretical and practical knowledge of the basic methodologies of the microbiological laboratory to allow the student the elements to choose to use and apply the appropriate methods of analysis for the diagnosis of infectious diseases. The course presents an in-depth study on the issues related to the relationship between the human body and microorganisms, using a medical perspective. Infections caused by some important human pathogens will be analyzed, with particular reference to the molecular mechanisms involved. Finally, the main diagnostic techniques used to identify the most important pathogens will be presented.
Prerequisitesfor the understanding of the course, students must be possess a minimum set of basic principles of cellular biology, chemistry, genetic, immunology and microbiology (minimal knowledge). They must also possess an English knowledge at level B1. Specific prerequisites are not foreseen.
Didattics Methodsfor the understanding of the course, students must be possess a minimum set of basic principles of cellular biology, chemistry, genetic, immunology and microbiology (minimal knowledge). They must also possess an English knowledge at level B1. Specific prerequisites are not foreseen. All the educational material is uploaded on the site https://www.dir.uniupo.it/ The teaching methods used by the course are represented by: - Presentations in MS-Power Point format - Screening of Peer Reviewed Scientific Video Journals (JoVE) - Use of the fast and fun "Kahoot" learning platform useful for ongoing assessment of learning - Laboratory theoretical-practical exercises to encourage students in their role as active participants The course of 5 total CFU (44 h) is organized as follows: - 32 hours of theoretical lectures, conducted in the classroom using power-point slides, classic blackboard and Kahoot platform, on all course topics. These lessons include a continuous direct examination in the classroom of the level of understanding of the various topics dealt with through dialogue, questions and kahoot in order to stimulate motivation, curiosity, critical spirit, autonomy and involvement in the learning processes of students. - 12 hours of theoretical-practical exercises (of which 6 in the form of visual experiments in the classroom and 6 in the didactic laboratory;these ones replicated in groups of about 25 people). These exercises allow students to put into practice and therefore apply the main microbiological issues and methodologies addressed in class. For practical exercises students are divided into groups of about 25 people.
Other informationsEmail: barbara.azzimonti@med.uniupo.it The teacher, at the end of the lessons or after contacting email, is available to discuss any doubts concerning the topics of the course. The teacher receives the students, by appointment, in his study c / o Palazzo Bellini, Department of Health Sciences, Via Solaroli 17, 28100 Novara. At the end of the course the exam will be simulated
Grading rulesSince the number of students is very high (groups A + B), the final evaluation test consists of a written exam, on the pre-established dates of the exams, as follows: - Fifteen multiple choice questions (more correct answer options; you must correctly answer to all the options to get 1 point/ question (total 15 points); points aren’t sottracted if answer opstions are not correctly marked - Two statements to be indicated as T / F (1.5 points) and motivated in 4-5 lines (6 points max), to obtain 7.5 points / question (total 15 points). The sum of the two scores must not be less than 18; the maximum achievable score is 30 cum laude. The questions focus on all the topics developed during the classroom lessons and practical laboratory exercises. The objective of the exam consists in verifying the level of achievement of the previously indicated educational objectives and in the ability to communicate and critically elaborate in writing the topics learned in italian language with appropriateness of language (correct use of technical-scientific terms). The examination also serves to evaluate the acquisition of knowledge, the ability to organize a structured response with clear clarity, the synthesis and appropriateness of language and the analytical / critical approach of the student towards the concepts learned during the course. A moment will be devoted to the correction of the written exams in which each student will be able to recognize its mistakes and identify the correct answers
Full argumentsSince the number of students is very high (groups A + B), the final evaluation test consists of a written exam, on the pre-established dates of the exams, as follows: - Fifteen multiple choice questions (more correct answer options; you must correctly answer to all the options to get 1 point/ question (total 15 points); points aren’t sottracted if answer opstions are not correctly marked - Two statements to be indicated as T / F (1.5 points) and motivated in 4-5 lines (6 points max), to obtain 7.5 points / question (total 15 points). The sum of the two scores must not be less than 18; the maximum achievable score is 30 cum laude. The questions focus on all the topics developed during the classroom lessons and practical laboratory exercises. The objective of the exam consists in verifying the level of achievement of the previously indicated educational objectives and in the ability to communicate and critically elaborate in writing the topics learned in italian language with appropriateness of language (correct use of technical-scientific terms). The examination also serves to evaluate the acquisition of knowledge, the ability to organize a structured response with clear clarity, the synthesis and appropriateness of language and the analytical / critical approach of the student towards the concepts learned during the course. A moment will be devoted to the correction of the written exams in which each student will be able to recognize its mistakes and identify the correct answers The bacterial cell: organization and structure. Division and growth. Bacterial metabolism. Resident microbial flora: the microbiota. Principles of bacterial virulence: Bacterial toxins: exotoxins and endotoxins; the spore; the capsule; the pili and the scourges; enzymes; the siderophores; the bacterial biofilm. Mechanisms of gene transfer: conjugation, transformation, transduction, transposition. Basics of the main pathogenic bacteria: Staphylococci, Streptococci, Enterobacteriaceae, Clostridia, Mycobacteria, Neisseria, Pseudomonas, A. Baumannii, Pneumococci, Chlamydia and Helicobacteria. Viruses: organization and structure, cultivation and multiplication, pathogenicity. Basics of the main pathogenic viruses (these topics will be explored during the Master's Degree in the Course of Molecular Virology): Hepadnavirus and other viruses causing hepatitis, Herpesvirus, Papillomavirus, Orthomyxovirus, Retrovirus. Basics of subviral agents: prions. Basics on eukaryotic microorganisms: the protists. Control of microbial growth: Disinfection and sterilization; antibiotics; natural antimicrobials; antiviral drugs; resistance mechanisms (superbugs). Vaccines: anti-polio, -meningitis, -difteritis, -tetanica, -pertosse, -rosolia, -HPV, -hepatitis, -influenza, -morbillo, -varicella. The immune response against infections. Aspecifica: inflammation, phagocytes, phagocytosis and killing, complement; Specification: humoral and cell-mediated. Evasion of immune response by microorganisms. Exercises Theoretical (visual experiments) Laboratory diagnosis of infectious diseases (microbiological techniques): plating of bacteria on solid media (selective, differential, selective / differential); Gram and Ziehl-Neelsen staining; Calculation of plaque forming units (CFU); tests of bacterial viability (XTT / MTT, Live / Dead assay, infrared assay); Biofilm analysis using Scanning Electron Microscopy (SEM); immunofluorescence and immunohistochemistry; fluorescent in situ hybridization (FISH); plaque assay; replica plate; the antibiogram, ELISA, PCR in microbiology. Practices: plating, identification of bacterial species, Gram and Ziehl-Neelsen stains, antibiogram, calculation of CFU and evaluation of bacterial metabolic activity by XTT.e assay
Expected learning objectivesLEARNING, KNOWLEDGE AND UNDERSTANDING: At the end of the course, the student should demonstrate knowledge of the structure, substructure and biological properties of bacteria, viruses and fungi and have understood, within the time frame, the main mechanisms underlying the infectious processes cause of human pathology. It should also demonstrate knowledge of the main techniques used in bacteriological and virological diagnostics. ABILITY TO APPLY KNOWLEDGE AND UNDERSTANDING: The student should have acquired skills in the field of medical microbiology that can be applied in laboratory practice. TRANSVERSAL SKILLS: The student must also be able to have JUDGMENT AUTONOMY and SKILLS to COMMUNICATE and motivate in writing and in practice his / her procedural / experimental choices.
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CourseBiochemistry
Course IDMS0737
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBALDANZI Gianluca
TeachersFERRARIS Davide Maria, CAPELLO Daniela, BERTONI Alessandra, BALDANZI Gianluca
CFU15
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year2
PeriodAnnuale
Grading typeFinal grade
Teaching languageItalian
AbstractIntroduction • Metabolism and bioenergy • Vitamins and coenzymes • Insulin and glucagon Functional Biochemistry 1. Carbohydrate digestion with aerobic and anaerobic glycolysis 2. Piruvate dehydrogenase and tricharboxylic acid cycle 3. Oxidative phosphorylation and ROS 4. Pentosophosphates + gluconeogenesis 5. Glycogen metabolism 6. Adjust glucose and glucose catabolism 7. Glycogenicity and glycemia 8. Lipids: digestion, transposed and lipoproteins, fatty acid catabolism 9. Catabolism odd C fatty acids, peroxisome ketone bodies and unsaturated fatty acids 10. Fatty acid and phospholipid biosynthesis with sphingolipid hints 11. Cholesterol: synthesis and catabolism to biliary acids 12. Steroid hormones, production and physiological roles 13. Photosynthesis and Calvin cycle 14. Organizing nitrogen and amino acid biosynthesis, essential amino acids 15. Protein digestion, blood transport (alanine, glutamine), amino acid degradation by example (tyrosine), urea cycle 16. Amino acid derivatives: bioactive amines, NO, eme 17. Anabolic Nucleotides and Dosoxinucleotides with Adjustment 18. Catabolism nucleotides and uric acid 19. Integration nutrition / fasting cycle metabolism. Laboratory Measuring biological things. Techniques for sample preparation in biochemistry Spectrophotometry. Chromatography Protein characterization. Using antibodies in biotechnology. Enzymes in diagnostic and research. Binding assays.
Reference textsPrincipi di biochimica di Lehninger di Lehninger - Nelson - Cox • 2014; VI edizione, Zanichelli Biochimica medica strutturale metabolica e funzionale di Noris Siliprandi, Guido Tettamanti. IV edizione, PICCIN PRINCIPI DI METODOLOGIA BIOCHIMICA di C. De Marco, C. Cini – Ed. Piccin METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI di REED Rob , HOLMES David , WEYERS Jonathan , JONES Allan - 2002 Zanichelli Editore METODOLOGIE DI BASE PER LA BIOCHIMICA E LA BIOTECNOLOGIA di NINFA Alexander J , BALLOU David P - 2000 Zanichelli Editore
Teaching targetsUnderstand and explain at the molecular, subcellular, cellular and tissue levels the biochemical mechanisms involved in digestion, absorption, transport, storage, catabolism, interconversion, excretion, biosynthesis of carbohydrates, amino acids and proteins, lipids, nucleotides, even in relation to different functional states of the body - Transfer the student to a solid knowledge of the hormonal regulation mechanisms of the major biochemical processes associated with the various biological functions. Learning basic methodologies in the biochemistry laboratory.
PrerequisitesMandatory: - general chemistry - organic chemistry - safe working in the laboratory Suggested: - physics
Didattics MethodsIn presence teaching with slide shows. Labwork. For the preparation of the exam, students will be able to use the material provided by the teacher (pdf copy of the slides delivered to the lesson and any deliveries that deepen the topics discussed during the course) and the recommended textbooks
Other informationsCourse program and slides are available on DIR (https://www.dir.uniupo.it/ ). The frequency of laboratory exercitations is mandatory. By the end of the exercitations, the student will present a laboratory book that will be evaluated together with student’s productivity in the laboratory.
Grading rulesThe exam consists of a written "structural biochemistry with elements of enzymology" and an oral "functional biochemistry with laboratory elements" reserved for those who have passed the written exam.
Full argumentsBioenergetics and types of biochemical reactions: Bioenergetics and thermodynamics. Chemical logic and most common biochemical reactions. Transfers of phosphorous and ATP groups. The biological reactions of oxidation reduction Glycolysis, gluconeogenesis and pathway of phosphate pentose: Glycolysis. Glycolysis supply routes. The fate of pyruvate under anaerobic conditions: fermentation. The gluconeogenesis. The oxidation of glucose through the pathway of pentose phosphate. Coordinated regulation of glycolysis and gluconeogenesis. Glycogen metabolism in animals, Coordinated regulation of glycogen synthesis and demolition The citric acid cycle: Production of acetyl-CoA (activated acetate). Citric acid cycle reactions. Adjustment of the citric acid cycle. The glyoxylate cycle Oxidative phosphorylation: Electron flow in mitochondria, ATP synthesis, Oxidative phosphorylation regulation, Mitochondria in thermogenesis, synthesis of steroids and apoptosis. Catabolism of fatty acids: Digestion, mobilization and transport of fatty acids. Oxygenation of fatty acids. The ketone bodies. Lipid Biosynthesis: Biosynthesis of fatty acids. Biosynthesis of triacylglycerols. 3 Membrane phospholipid biosynthesis. Cholesterol, steroids and isoprenoids: biosynthesis, regulation and transport Oxidation of amino acids and production of urea: Metabolic Destiny of Amine Groups. Nitrogen excretion and urea cycle. Degradation routes of amino acids. Biosynthesis of amino acids, nucleotides and related molecules PHOTOSYSTEMS: LIGHTING ENERGY CATTURE: Light absorption and photophosphorylation, The central photochemical event: light-induced electron flow. Synthesis of ATP coupled to photophosphorylation. Carbohydrate biosynthesis in plants and bacteria Metabolic control principles: Metabolic pathway regulation. Analysis of metabolic control. Hormone regulation and metabolism integration into mammals. Meaning of the measure in the biological field: specificity, accuracy, precision and reproducibility. Sample preparation techniques for biochemical analyzes (homogenization, lysis with detergents). Extraction with solvents and selective precipitation, their application to the fractionation of biological compounds (extraction of lipids, salting out of proteins and nucleic acids). Centrifugation theory and preparative centrifugation techniques for the fractionation of biological compounds. Use of centrifuges and centrifugal force. Principles of spectrophotometry. The theoretical bases and the practical aspects of spectroscopic techniques (absorption, fluorescence, polarimetry) and examples of their use for the quantification of biological analytes will be discussed. Characteristics of a spectrophotometer and a fluorimeter, their use. Radioisotopes in the laboratory: characteristics of radioisotopes of the most common use, their uses in biochemistry and instrumentation used for their quantification. Principles underlying chromatography, main chromatogical techniques (affinity, gel filtration, ion exchange and reverse phase) and relative matrices. Use of chromatography for the separation of complex mixtures of proteins and lipids based on chemical and physical properties. Instrumentation used (column chromatography, thin layer, HPLC, etc.). Detectors and results analysis (retention coefficients, qualitative and quantitative analyzes). Principles at the base of electrophoresis with particular attention to fractionation and characterization of proteins. IEF, SDS-PAGE, 2D-PAGE, capillary electrophoresis. Most common detection systems: dyes and western blotting. Protein characterization: digestion, Edman sequencing, use of mass spectrometry for the characterization of proteins, qualitative and quantitative proteomics with particular attention to mass spectrometry techniques. Antibody applications in biotechnology, competitive and non-competitive immunological assays, RIA, ELISA, immunofluorescence, western blotting, immunodiffusion and related techniques. Use of enzymes in diagnostics and research. Characterization of enzymatic kinetics and study of inhibitors. Use of enzymes in clinical diagnostics, kinetic assays and end-points applied to research and diagnostics. Analysis of kinetic parameters of an enzyme. Binding and binding assays, receptor-ligand equilibrium analysis. Methods for the study of molecular interactions, classical essays with marked molecules, advanced spectrometric methods: Fret, Bret, time resolved fluorescence.
Expected learning objectivesAn in-depth knowledge of metabolism, biotechnological implications, and metabolic regulation. The student will acquire the knowledge of the main biochemical methods with particular emphasis on the study of proteins and critical reading of literature. Exercises allow you to move efficiently in the lab even in view of the final thesis preparation.
Modules
Course ID Course SSD Teachers
MS0738 Structural biochemistry and fundamental of enzimology BIO/10 - BIOCHIMICA CAPELLO Daniela, FERRARIS Davide Maria
MS0739 Functional biochemistry and laboratory BIO/10 - BIOCHIMICA BALDANZI Gianluca, BERTONI Alessandra
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CourseStructural biochemistry and fundamental of enzimology
Course IDMS0738
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCAPELLO Daniela
TeachersFERRARIS Davide Maria, CAPELLO Daniela
CFU6
Teaching duration (hours)48
Individual study time 102
SSDBIO/10 - BIOCHIMICA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodPrimo Semestre
Grading typeFinal grade
Sites and/or partitions
Gruppo B
Gruppo A
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CourseStructural biochemistry and fundamental of enzimology
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderFERRARIS Davide Maria
TeachersFERRARIS Davide Maria
Course typeGruppi
Year2
PartitionGruppo B
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CourseStructural biochemistry and fundamental of enzimology
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCAPELLO Daniela
TeachersCAPELLO Daniela
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
AbstractChemical bonds and interactions between molecules in the chemistry of life. Structure, classification and function of biological macromolecules. Simple and complex glycides; lipids and structure of biological membranes. Nucleic acids and general mechanisms of information transmission. Amino acids and proteins: structures and folding. Co and post-translational modifications of proteins and signals for localization. Cellular addressing processes. Enzymes and enzymatic kinetics- Extracellular transport proteins, proteins that bind oxygen, iron and copper. Contractile structural unit: morphological and molecular organization of muscle fiber, myofibrils and sarcomere. Proteins of the cytoskeleton Protein of the extracellular matrix: laminins, fibronectin, collagens, elastin. Transport of molecules: biochemistry of vesicular traffic - Transport of molecules through the membranes: transporters and channels. Transmission of regulatory signals from outside into the cell: fundamental mechanisms of signal transduction. The main signaling pathways in multicellular organisms
Reference textsDonald Voet, Judith G Voet, Charlotte W Pratt. PRINCIPI DI BIOCHIMICA, 2017. Zanichelli Duranti. Introduzione allo studio delle proteine. Zanichelli Donald Voet, Judith G Voet, Charlotte W Pratt. FONDAMENTI DI BIOCHIMICA. IV edition, 2017. Zanichelli David L Nelson, Michael M Cox. PRINCIPI DI BIOCHIMICA DI LEHNINGER. VII Edition, 2018. Zanichelli Umberto Mura. ENZIMI IN AZIONE. EdiSES, 2012
Teaching targetsTransmit to the student the bases for a molecular approach to physiology and pathology, through the definition of the structure-function relationship of biological macromolecules, with particular attention to proteins with enzymatic activity, to transport proteins and to those involved in signal transduction. At the end of the course the student must be able to describe the molecular bases of macromolecules structures, the mechanisms of action of enzymes and an adequate knowledge of the mechanisms of signal transduction.
PrerequisitesBasic knowledge of general chemistry, organic chemistry, physics and cell biology
Didattics MethodsFrontal lesson with the projection of slides, and movies. Execution of problems and quizzes in the classroom. Ability to run problems and quizzes at home via the moodle platform. Students can also use the recommended textbooks to deepen the topics discussed in the classroom
Other informationsA pdf copy of the projected slides, the in-depth material and all the information regarding the course and the exam procedures will be made available on the DIR. The teacher is available, by appointment, to provide clarification on specific topics even outside of class time.
Grading rulesIn itinere tests will be carried out during the course. These tests will NOT be used for final evaluation purposes but will allow the student to assess his level of understanding of the different topics and to practice for passing the examination. The aim of the exam consists in verifying the level of knowledge of the program topics and the reasoning skills developed by the student. The exam consists of a written test including 50 questions related to all the topics covered during the course and including quizzes with multiple choice or true / false answers, recognition of molecular structures, and numerical problems. For each correct answer, 0.66 points are awarded. Sufficiency is reached by correctly answering to 27/50 questions. The evaluation is expressed in thirtieths (minimum mark 18, maximum 30 and honors). During the written test it is not allowed to consult any kind of material. The calculator is allowed. The examination allows to evaluate the critical sense, the ability to understand the text and the capacity to apply the logic and acquired specialized terminology to recognize and describe the biochemical processes (questions where it is required to make a choice between different alternatives) and the problem-solving skills by applying knowledge to the resolution of technical experimental problems (numerical questions).
Full arguments1_ The biological macromolecules. The chemical bonds and the interactions between molecules in the chemistry of life. Carbohydrates. Monosaccharides, disaccharides, glycosidic bond. Omopolysaccharides, modified monosaccharides, heteropolisaccharides: glycosaminoglycans, chitin, agar, hemicellulose, gums. Glycoconjugates: glipicans, sindecans, proteoglycans, glycoproteins. Lipids. Saturated and unsaturated fatty acids. Neutral glycerides and waxes, glycerophospholipids, plasmalogens, sphingolipids, glycolipids. Principles of organization of biological membranes. Cholesterol structure and its derivatives. Nucleic acids. Nitrogenous bases, nucleosides, nucleotides. The phosphodiester bond, primary and secondary structure of nucleic acids. DNA denaturation and duplication of information. Amino acids: structure, stereoisomerism and chirality. Reversible modifications of amino acids involved in protein regulation. Isoelectric point. Proteins. Peptide bond: chemical and physical properties. Outline of the derived molecules of amino acids with biological activity. The directionality of the amino acid sequence; hints on the conversion of the genetic code into an amino acid sequence (translation of mRNA). Secondary structures, tertiary structure. Bridge disulfide bonds. Supersecondary structures, structural motifs and functional domains. Tertiary structure. Folding. Quaternary structure: fibrous proteins and globular proteins. Immunoenzymatic methods for the study of protein structure: immunoblot / Western Blot, Enzyme Linked Immuno Sorbent Assay (ELISA) 2_Enzymes and enzymatic kinetics. The laws of thermodynamics in biological reactions. Activation energy its impact on the reaction speed. Speed constant. Enzymes, coenzymes, prosthetic groups, co-substrates: general mechanisms of action. Classification of enzymes. Main catalysis mechanisms. Enzymatic kinetics. Steady state hypothesis, Michaelis-Menten equation. Basic kinetic parameters: turnover number, specificity constant, meaning and calculation method. Competitive, mixed and incompetitive inhibition: kinetic aspects. Mechanisms of general regulation of enzymes: covalent and non-covalent; allostery. Regulation by proteolysis: serin-protease, cysteine-protease, aspartyl-protease, metalloprotease3 Relationship structure-localization-function of biological macromoleculesOrganization of cellular and subcellular membranes; movement of lipids through the double layer. The fluid mosaic model. The "lipid rafts" / lipid rafts. Post-translational modifications of proteins and signals for localization. Signals of entry and retention in organelles or of addressing to the plasma membrane. Proteins that bind O2: globins; structure of the EME group. Structure and function of myoglobin: and hemoglobin. Saturation curve. Allosteric T / R variants, cooperativity. Equation and coefficient of Hill. Regulation of the affinity of Hb for its ligand by: O2, CO2, H + and their role in the gaseous exchanges at the pulmonary and tissue level. Role of 2,3 bisphosphoglycerate (BPG) in the regulation of Hb affinity for O2. Molecular basis of thalassemia, hemoglobinopathies and meteglobinemia. Proteins with recognition and defense function: immunoglobulins: general classification, structure and mechanisms of interaction with the antigen. Overview of the genetic rearrangement that generates the variable regions of heavy and light chains. Contractile structural unit: morphological and molecular organization of muscle fiber, myofibrils and sarcomere. Biochemical contraction mechanism: regulation of acto-myosin complex activity, role of ATP and calcium ions Cytoskeletal proteins. Actin polymerization mechanism, proteins that bind the actin and regulate its polymerization. Microtubules: structure, mechanism of polymerization and intracellular role. Molecular motors: chinesine and dineins. Structure and ATP-dependent progress mechanism on the microtubule. Intermediate filaments: functional characteristics and polymerization mechanism. Extracellular matrix proteins: laminins, fibronectin, collagens, elastin. Transport of molecules through the membranes: biochemistry of vesicular traffic. Overview of the general mechanisms of endocytosis. Bending mechanisms and cleavage of membranes. Endocytosis mediated by clathrin and non-clathrin-dependent receptors. Transportation of molecules through membranes: transporters and channels. General mechanisms of transport through the membranes. Transport not mediated; transporters and channels, facilitated transport, and active transport. Ionic pumps. Co-transport and anti-charge systems. The ABC carriers. Excitable membranes, action potentials and neurotransmission. Voltage-dependent channels. Ionic channels controlled by ligands: the acetylcholine channel receptor; channel desensitization mechanisms. 4_ Transduction of regulatory signals from the outside into the cell. General principles of interaction of signal molecules (ligands) with their specific receptors. Classification of reporting types. Signal proteins and second messengers. Formation of signaling complexes. Characteristics of the binding process and signal extinguishing mechanisms. Cell-cell adhesion and cell-matrix adhesion receptors. Structure and function of adhesion molecules ("Cell Adhesion Molecules", CAM) IgSF CAM, selectins and caderins. Function of caderins in cell-cell junction complexes and role of beta-catenin in signaling. Integrins: interaction with the extra cellular matrix in the formation of focal adhesions and emidesmosomes. G protein-coupled receptors (GPCR): structure and mechanism of action of GPCR and of heterotrimeric G proteins: coupling of G protein / effectors; molecular mechanism of receptor desensitization. Effector systems of heterotrimeric proteins G producing second intracellular messengers: adenylate cyclase, cyclic-AMP (cAMP), Protein Kinase A (PKA). Phosphodiesterases. Phospholipases C: diacylglycerol (DAG) and Inositol trisphosphate (IP3). Ca2 + -dependent signaling mechanisms. Tyrosine kinase receptors (TKR): structural structure and general signaling mechanism. Role of the SH2 / phosphotyrosine domain interaction in the recruitment and activation of the signal transduction pathways. Signaling downstream of TKR receptors: pathways of the small G-proteins (Ras and members of the Ras superfamily), of MAP-kinases and of phosphatidylinositol 3 kinases (PI-3K). Role of PKB / Akt in the PI-3K-dependent signaling Soluble tyrosine-kinase proteins. The SRC family, structure and mechanism of regulation. Receptors associated with tyrosine kinase: general classification of receptors for cytokines and glycoprotein hormones, the pathway of JAK / STAT. Serine and threonine kinase receptor: the TGF-b receptor family. receptors with cyclase guanylate activity; soluble guanilate cyclase NO-dependent. NO-synthase. Functions of cGMP. Biological actions of NO mediated by cGMP. Inhibition of cGMP-dependent pathways Intracellular receptors: general mechanisms of action.
Expected learning objectivesTo be able to identify biological macromolecules, to catch the interconnections between macromolecular structures, function and regulation and to acquire independent judgment in the evaluation of biochemical problems related to the structure and functioning of the biomolecules within the cell and in the extracellular environment. Know the mechanisms of enzyme activity and know how to solve simple problems related to enzymatic kinetics. Possess the basic knowledge of signal transduction and of cellular transport and mobility mechanisms
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CourseFunctional biochemistry and laboratory
Course IDMS0739
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBALDANZI Gianluca
TeachersBERTONI Alessandra, BALDANZI Gianluca
CFU9
Teaching duration (hours)48
Individual study time 153
SSDBIO/10 - BIOCHIMICA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodSecondo Semestre
Sites and/or partitions
Gruppo B
Gruppo A
Close course details
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CourseFunctional biochemistry and laboratory
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBERTONI Alessandra
TeachersBERTONI Alessandra
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
AbstractIntroduzione • Metabolismo e bioenergetica • Vitamine e coenzimi • Insulina e glucagone Biochimica Funzionale 1. Digestione carboidrati con glicolisi aerobia ed anaerobia 2. Piruvato deidrogenasi e ciclo di acidi tricarbossilici 3. Fosforilazione ossidativa e ROS 4. Pentoso fosfati + gluconeogenesi 5. Metabolismo glicogeno 6. Regolazione catabolismo glucosio e glicemia 7. Regolazione glicogeno e glicemia 8. Lipidi: digestione, trasposto e lipoproteine, catabolismo acidi grassi 9. Catabolismo acidi grassi C dispari, corpi chetonici perossisomi ed acidi grassi insaturi 10. Biosintesi acidi grassi e fosfolipidi con accenni agli sfingolipidi 11. Colesterolo: sintesi e catabolismo ad acidi biliari 12. Ormoni steroidei, produzione e ruoli fisiologici 13. Fotosintesi e ciclo di Calvin 14. Organicazione dell’azoto e biosintesi aminoacidi, aminoacidi essenziali 15. Digestione proteine, trasporto nel sangue (alanina, glutammina), degradazione degli aminoacidi per esempi (tirosina), ciclo dell’urea 16. Derivati degli aminoacidi: ammine bioattive, NO, eme 17. Anabolismo nucleotidi e desossinucleotidi con regolazione 18. Catabolismo nucleotidi e acido urico 19. Integrazione metabolismo ciclo nutrito/digiuno. Laboratorio Significato della misura in ambito biologico. Tecniche di preparazione del campione. Principi di spettrofotometria. Radioisotopi in laboratorio biomedico. Principi alla base della cromatografia. Principi alla base dell'elettroforesi. Tecniche per la caratterizzazione di proteine. Applicazioni degli anticorpi nelle biotecnologie. Utilizzo di enzimi in diagnostica ed in ricerca. Saggi di binding ed associazione.
Reference textsPrincipi di biochimica di Lehninger di Lehninger - Nelson - Cox • 2014; VI edizione, Zanichelli Biochimica medica strutturale metabolica e funzionale di Noris Siliprandi, Guido Tettamanti. IV edizione, PICCIN PRINCIPI DI METODOLOGIA BIOCHIMICA di C. De Marco, C. Cini – Ed. Piccin METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI di REED Rob , HOLMES David , WEYERS Jonathan , JONES Allan - 2002 Zanichelli Editore METODOLOGIE DI BASE PER LA BIOCHIMICA E LA BIOTECNOLOGIA di NINFA Alexander J , BALLOU David P - 2000 Zanichelli Editore
Teaching targetsConoscere e spiegare a livello molecolare, subcellulare, cellulare e tissutale i meccanismi biochimici coinvolti nei processi di: digestione, assorbimento, trasporto, deposito, catabolismo, interconversioni, escrezione, biosintesi di: carboidrati, amminoacidi e proteine, lipidi, nucleotidi, gruppo eme, anche in relazione a diversi stati funzionali dell’organismo - Trasferire allo studente solide conoscenze dei meccanismi di regolazione ormonale dei principali processi biochimici associati alle diverse funzioni biologiche. Le lezioni teoriche forniscono allo studente una panoramica delle tecniche biochimiche moderne e delle loro applicazioni com particolare riguardo allo studio delle proteine. Allo stesso tempo il modulo teorico vuole fornire allo studente gli strumenti per una lettura critica della letteratura. Le esercitazioni mirano a far acquisire le capacità pratiche richieste per l’utilizzo delle tecniche biochimiche di base e per un proficuo internato di tesi.
PrerequisitesObbligatori: - chimica generale - chimica organica - sicurezza in laboratori chimici Suggeriti: - fisica
Didattics MethodsLezioni frontali con proiezione di diapositive. Esercitazioni in laboratori didattici. Per la preparazione dell'esame gli studenti potranno utilizzare il materiale fornito dal docente (copia pdf delle diapositive proiettate a lezione ed eventuali dispense che approfondiscono gli argomenti trattati durante il corso) ed i libri di testo consigliati
Other informationsCopia pdf delle diapositive proiettate, il materiale di approfondimento e tutte le informazioni riguardanti il corso e le modalità di esame saranno rese disponibili sul DIR (https://www.dir.uniupo.it/ ). Al termine delle esercitazioni lo studente presenterà un quaderno di laboratorio che insieme al rendimento in laboratorio costituirà la base per la successiva valutazione. Si ricorda che la frequenza dei laboratori didattici è obbligatoria.
Grading rulesL’esame è orale. Il voto dipende dal grado di raggiungimento degli obiettivi formativi.
Full argumentsBioenergetica e tipi di reazioni biochimiche: Bioenergetica e termodinamica. Logica chimica e reazioni biochimiche più comuni. Trasferimenti di gruppi fosforici e ATP. Le reazioni biologiche di ossidoriduzione Glicolisi, gluconeogenesi e via del pentosio fosfato: La glicolisi. Vie di alimentazione della glicolisi. Il destino del piruvato in condizioni anaerobiche: la fermentazione. La gluconeogenesi. L’ossidazione del glucosio attraverso la via del pentosio fosfato. Regolazione coordinata della glicolisi e della gluconeogenesi. Il metabolismo del glicogeno negli animali, Regolazione coordinata della sintesi e della demolizione del glicogeno Il ciclo dell’acido citrico: Produzione di acetil-CoA (acetato attivato). Reazioni del ciclo dell’acido citrico. Regolazione del ciclo dell’acido citrico. Il ciclo del gliossilato Fosforilazione ossidativa: Il flusso degli elettroni nei mitocondri, La sintesi dell’ATP, Regolazione della fosforilazione ossidativa, I mitocondri nella termogenesi, nella sintesi degli steroidi e nell’apoptosi. Catabolismo degli acidi grassi: Digestione, mobilizzazione e trasporto degli acidi grassi. Ossidazione degli acidi grassi. I corpi chetonici. Biosintesi dei lipidi: Biosintesi degli acidi grassi. Biosintesi dei triacilgliceroli. 3 Biosintesi dei fosfolipidi di membrana. Colesterolo, steroidi e isoprenoidi: biosintesi, regolazione e trasporto Ossidazione degli amminoacidi e produzione dell’urea: Destino metabolico dei gruppi amminici. Escrezione dell’azoto e ciclo dell’urea. Vie di degradazione degli amminoacidi. Biosintesi degli amminoacidi, dei nucleotidi e delle molecole correlate FOTOSINTESI: LA CATTURA DELL’ENERGIA LUMINOSA: L’assorbimento della luce e fotofosforilazione, L’evento fotochimico centrale: il flusso di elettroni indotto dalla luce. Sintesi di ATP accoppiata alla fotofosforilazione. Biosintesi dei carboidrati nelle piante e nei batteri Principi di regolazione metabolica: Regolazione delle vie metaboliche. Analisi del controllo metabolico. Regolazione ormonale e integrazione del metabolismo nei mammiferi. Significato della misura in ambito biologico: specificità, accuratezza, precisione e riproducibilità. Tecniche di preparazione del campione per analisi biochimiche (omogenizzazione, lisi con detergenti). Estrazione con solventi e precipitazione selettiva, loro applicazione al frazionamento dei composti biologici (estrazione di lipidi, salting out di proteine ed acidi nucleici). Teoria della centrifugazione e tecniche di centrifugazione preparativa per il frazionamento di composti biologici. Utilizzo delle centrifughe e forza centrifuga. Principi di spettrofotometria. Saranno trattate le basi teoriche e gli aspetti pratici delle tecniche spettroscopiche (assorbimento, fluorescenza, polarimetria) ed esempi del loro utilizzo per la quantificazione di analiti biologici. Caratteristiche di uno spettrofotometo ed un fluorimetro, loro utilizzo. Radioisotopi in laboratorio: caratteristiche dei radioisotopi di uso più comune, loro utilizzi in biochimica e strumentazione utilizzata per la loro quantificazione. Principi alla base della cromatografia, principali tecniche cromatogafiche (affinià, gel filtrazione, scambio ionico e fase inversa) e relative matrici. Utilizzo della cromatografia per la separazione di miscele complesse di proteine e di lipidi in base alle proprietà chimiche e fisiche. Strumentazione utilizzata (cromatografie su colonna, su strato sottile, HPLC, ecc). Rivelatori ed analisi risultati (coefficienti di ritenzione, analisi qualitative e quantitative). Principi alla base dell'elettroforesi con particolare attenzione al frazionamento e caratterizzazione delle proteine. IEF, SDS-PAGE, 2D-PAGE, elettroforesi capillare. Sistemi di rilevazione più comuni: coloranti e western blotting. Caratterizzazione di proteine: digestione, sequenziamento di Edman, utilizzo della spettrometria di massa per la caratterizzazione delle proteine, proteomica qualitativa e quantitativa con particolare attenzione alle tecniche di spettrometria di massa. Applicazioni degli anticorpi nelle biotecnologie, saggi immunologici competitivi e non competitivi, RIA, ELISA, immunofluorescenza, western blotting, immunodiffusione e tecniche correlate. Utilizzo di enzimi in diagnostica ed in ricerca. Caratterizzazione della cinetica enzimatica e studio degli inibitori. Utilizzo di enzimi in diagnostica clinica, assay cinetici ed end-point applicati alla ricerca ed alla diagnostica. Analisi di parametri cinetici di un enzima. Saggi di binding ed associazione, analisi dell’equilibrio recettore-ligando. Metodiche per lo studio delle interazioni molecolari, saggi classici con molecole marcate, metodiche spettrometriche avanzate: Fret, Bret, fluorescenza a tempo risolto.
Expected learning objectivesUna conoscenza approfondita del metabolismo, delle implicazioni biotecnologiche e della regolazione metabolica. Lo studente acquisirà la conoscenza delle principali metodologie biochimiche con particolare enfasi allo studio delle proteine e alla lettura critica della letteratura. Le esercitazione consentono di muoversi fruttuosamente in laboratorio anche in vista dell'elaborato finale.
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CourseFunctional biochemistry and laboratory
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBALDANZI Gianluca
TeachersBALDANZI Gianluca
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
AbstractIntroduction • Metabolism and bioenergy • Vitamins and coenzymes • Insulin and glucagon Functional Biochemistry 1. Carbohydrate digestion with aerobic and anaerobic glycolysis 2. Piruvate dehydrogenase and tricharboxylic acid cycle 3. Oxidative phosphorylation and ROS 4. Pentosophosphates + gluconeogenesis 5. Glycogen metabolism 6. Adjust glucose and glucose catabolism 7. Glycogenicity and glycemia 8. Lipids: digestion, transposed and lipoproteins, fatty acid catabolism 9. Catabolism odd C fatty acids, peroxisome ketone bodies and unsaturated fatty acids 10. Fatty acid and phospholipid biosynthesis with sphingolipid hints 11. Cholesterol: synthesis and catabolism to biliary acids 12. Steroid hormones, production and physiological roles 13. Photosynthesis and Calvin cycle 14. Organizing nitrogen and amino acid biosynthesis, essential amino acids 15. Protein digestion, blood transport (alanine, glutamine), amino acid degradation by example (tyrosine), urea cycle 16. Amino acid derivatives: bioactive amines, NO, eme 17. Anabolic Nucleotides and Dosoxinucleotides with Adjustment 18. Catabolism nucleotides and uric acid 19. Integration nutrition / fasting cycle metabolism. Laboratory Measuring biological things. Techniques for sample preparation in biochemistry Spectrophotometry. Chromatography Protein characterization. Using antibodies in biotechnology. Enzymes in diagnostic and research. Binding assays.
Reference textsPrincipi di biochimica di Lehninger di Lehninger - Nelson - Cox • 2014; VI edizione, Zanichelli Biochimica medica strutturale metabolica e funzionale di Noris Siliprandi, Guido Tettamanti. IV edizione, PICCIN PRINCIPI DI METODOLOGIA BIOCHIMICA di C. De Marco, C. Cini – Ed. Piccin METODOLOGIE DI BASE PER LE SCIENZE BIOMOLECOLARI di REED Rob , HOLMES David , WEYERS Jonathan , JONES Allan - 2002 Zanichelli Editore METODOLOGIE DI BASE PER LA BIOCHIMICA E LA BIOTECNOLOGIA di NINFA Alexander J , BALLOU David P - 2000 Zanichelli Editore
Teaching targetsUnderstand and explain at the molecular, subcellular, cellular and tissue levels the biochemical mechanisms involved in digestion, absorption, transport, storage, catabolism, interconversion, excretion, biosynthesis of carbohydrates, amino acids and proteins, lipids, nucleotides, even in relation to different functional states of the body - Transfer the student to a solid knowledge of the hormonal regulation mechanisms of the major biochemical processes associated with the various biological functions. Learning basic methodologies in the biochemistry laboratory.
PrerequisitesMandatory: - general chemistry - organic chemistry - safe working in the laboratory Suggested: - physics
Didattics MethodsIn presence teaching with slide shows. Labwork. For the preparation of the exam, students will be able to use the material provided by the teacher (pdf copy of the slides delivered to the lesson and any deliveries that deepen the topics discussed during the course) and the recommended textbooks
Other informationsCourse program and slides are available on DIR (https://www.dir.uniupo.it/ ). The frequency of laboratory exercitations is mandatory. By the end of the exercitations, the student will present a laboratory book that will be evaluated together with student’s productivity in the laboratory.
Grading rulesThe exam is oral. The vote depends on the degree of achievement of the training objectives.
Full argumentsBioenergetics and types of biochemical reactions: Bioenergetics and thermodynamics. Chemical logic and most common biochemical reactions. Transfers of phosphorous and ATP groups. The biological reactions of oxidation reduction Glycolysis, gluconeogenesis and pathway of phosphate pentose: Glycolysis. Glycolysis supply routes. The fate of pyruvate under anaerobic conditions: fermentation. The gluconeogenesis. The oxidation of glucose through the pathway of pentose phosphate. Coordinated regulation of glycolysis and gluconeogenesis. Glycogen metabolism in animals, Coordinated regulation of glycogen synthesis and demolition The citric acid cycle: Production of acetyl-CoA (activated acetate). Citric acid cycle reactions. Adjustment of the citric acid cycle. The glyoxylate cycle Oxidative phosphorylation: Electron flow in mitochondria, ATP synthesis, Oxidative phosphorylation regulation, Mitochondria in thermogenesis, synthesis of steroids and apoptosis. Catabolism of fatty acids: Digestion, mobilization and transport of fatty acids. Oxygenation of fatty acids. The ketone bodies. Lipid Biosynthesis: Biosynthesis of fatty acids. Biosynthesis of triacylglycerols. 3 Membrane phospholipid biosynthesis. Cholesterol, steroids and isoprenoids: biosynthesis, regulation and transport Oxidation of amino acids and production of urea: Metabolic Destiny of Amine Groups. Nitrogen excretion and urea cycle. Degradation routes of amino acids. Biosynthesis of amino acids, nucleotides and related molecules PHOTOSYSTEMS: LIGHTING ENERGY CATTURE: Light absorption and photophosphorylation, The central photochemical event: light-induced electron flow. Synthesis of ATP coupled to photophosphorylation. Carbohydrate biosynthesis in plants and bacteria Metabolic control principles: Metabolic pathway regulation. Analysis of metabolic control. Hormone regulation and metabolism integration into mammals. Meaning of the measure in the biological field: specificity, accuracy, precision and reproducibility. Sample preparation techniques for biochemical analyzes (homogenization, lysis with detergents). Extraction with solvents and selective precipitation, their application to the fractionation of biological compounds (extraction of lipids, salting out of proteins and nucleic acids). Centrifugation theory and preparative centrifugation techniques for the fractionation of biological compounds. Use of centrifuges and centrifugal force. Principles of spectrophotometry. The theoretical bases and the practical aspects of spectroscopic techniques (absorption, fluorescence, polarimetry) and examples of their use for the quantification of biological analytes will be discussed. Characteristics of a spectrophotometer and a fluorimeter, their use. Radioisotopes in the laboratory: characteristics of radioisotopes of the most common use, their uses in biochemistry and instrumentation used for their quantification. Principles underlying chromatography, main chromatogical techniques (affinity, gel filtration, ion exchange and reverse phase) and relative matrices. Use of chromatography for the separation of complex mixtures of proteins and lipids based on chemical and physical properties. Instrumentation used (column chromatography, thin layer, HPLC, etc.). Detectors and results analysis (retention coefficients, qualitative and quantitative analyzes). Principles at the base of electrophoresis with particular attention to fractionation and characterization of proteins. IEF, SDS-PAGE, 2D-PAGE, capillary electrophoresis. Most common detection systems: dyes and western blotting. Protein characterization: digestion, Edman sequencing, use of mass spectrometry for the characterization of proteins, qualitative and quantitative proteomics with particular attention to mass spectrometry techniques. Antibody applications in biotechnology, competitive and non-competitive immunological assays, RIA, ELISA, immunofluorescence, western blotting, immunodiffusion and related techniques. Use of enzymes in diagnostics and research. Characterization of enzymatic kinetics and study of inhibitors. Use of enzymes in clinical diagnostics, kinetic assays and end-points applied to research and diagnostics. Analysis of kinetic parameters of an enzyme. Binding and binding assays, receptor-ligand equilibrium analysis. Methods for the study of molecular interactions, classical essays with marked molecules, advanced spectrometric methods: Fret, Bret, time resolved fluorescence.
Expected learning objectivesAn in-depth knowledge of metabolism, biotechnological implications, and metabolic regulation. The student will acquire the knowledge of the main biochemical methods with particular emphasis on the study of proteins and critical reading of literature. Exercises allow you to move efficiently in the lab even in view of the final thesis preparation.
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CourseHuman physiology
Course IDBT035
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGROSSINI Elena
TeachersGROSSINI Elena, LIM Dmitry
CFU5
Teaching duration (hours)40
Individual study time 85
SSDBIO/09 - FISIOLOGIA
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseHuman physiology
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGROSSINI Elena
TeachersGROSSINI Elena, LIM Dmitry
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
AbstractThe main focus of the course of Physiology is to achieve the knowledge of principles at basis of functional activity of various organs.The cardiovascular, respiratory, renal, digestive and endocrine systems will be treated. As regarding the nervous system, its role in the regulation of above systems will be treated.
Reference textsFisiologia Umana by F. Grassi, D. Negrini and CA Porro, Poletto Editore
Teaching targetsThe educational objectives are addressed to the knowledge of principles governing the functional activity of various organs and apparatus and to match them with main cellular mechanisms.Specific objectives: knowledge of miocardic properties; knowledge of the onset of rest membrane potential and of action potential in pacemaker cells and miocardic cells; definition of systolic output and cardiac output; general knoweledge of cardiac cycle and arterial blood pressure; knowledge of mechanisms of breathing, main respiratory volumes and respiratory capacity; alveolar gas exchange and blood transport; mechanisms of glomerular filtration, tubular reabsorption and secretion; renal clearances: definition and main clearances values; general knowledge of mechanisms of water and glucose reabsorption; knowledge of mechanisms of production of gastrointestinal secretes and their composition; knowledge of mechanisms involved in gastrointestinal absorption and of liver function; knowledge of hypothalamic-hypophysis axis and of function of hormones produced by tyroid, parathyroids, pancreas, adrenal glands and gonades.
PrerequisitesStudents should be in possession of basic knowledge of Anatomy, Istology, Chemistry and Biochemistry.
Didattics MethodsPower point
Grading rulesWritten test (true/false) on the entire program
Full argumentsCardiovascular system: properties of the myocardium, cardiac cycle, cardiac output, arterial blood pressure. Respiratory system:respiratory mechanic; alveolar ventilation, alveolar gas exchange, chemical and nervous respiratory control, blood gas transport. Renal system:glomerular filtration, re-absorbption and secretion mechanisms, renal clearances. Digestive system: composition, production and role of digestive secretes, intestinal absorbption, liver function. Endocrine system: physiologic functions of hormones released by hypothalamus, pituitary gland, thyroid, parathyroid glands, surrenal glands, endocrine pancreas and gonades. Nervous system: the neurons
Expected learning objectivesThe student should: (KNOWLEDGE) -be aware of basic physiologic mechanisms that regulate the function of organs (COMPETENCE) -use the knowledge for solving main biotechnologic issues (TRANSVERSAL ABILITY) -to be able to read, understand and discuss technical material taken from books, other tests etc; -to know or find out the international terminology
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CourseHuman physiology
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGROSSINI Elena
TeachersGROSSINI Elena, LIM Dmitry
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
AbstractThe main focus of the course of Physiology is to achieve the knowledge of principles at basis of functional activity of various organs.The cardiovascular, respiratory, renal, digestive and endocrine systems will be treated. As regarding the nervous system, its role in the regularion of above systems will be treated.
Reference textsFisiologia Umana by F. Grassi, D. Negrini and CA Porro, Poletto Editore
Teaching targetsThe educational objectives are addressed to the knowledge of principles governing the functional activity of various organs and apparatus and to match them with main cellular mechanisms.Specific objectives: knowledge of miocardic properties; knowledge of the onset of rest membrane potential and of action potential in pacemaker cells and miocardic cells; definition of systolic output and cardiac output; general knoweledge of cardiac cycle and arterial blood pressure; knowledge of mechanisms of breathing, main respiratory volumes and respiratory capacity; alveolar gas exchange and blood transport; mechanisms of glomerular filtration, tubular reabsorption and secretion; renal clearances: definition and main clearances values; general knowledge of mechanisms of water and glucose reabsorption; knowledge of mechanisms of production of gastrointestinal secretes and their composition; knowledge of mechanisms involved in gastrointestinal absorption and of liver function; knowledge of hypothalamic-hypophysis axis and of function of hormones produced by tyroid, parathyroids, pancreas, adrenal glands and gonades.
PrerequisitesStudents should be in possession of basic knowledge of Anatomy, Istology, Chemistry and Biochemistry.
Didattics MethodsPower point
Grading rulesWritten test (true/false) on the entire program
Full argumentsCardiovascular system: properties of the myocardium, cardiac cycle, cardiac output, arterial blood pressure. Respiratory system:respiratory mechanic; alveolar ventilation, alveolar gas exchange, chemical and nervous respiratory control, blood gas transport. Renal system:glomerular filtration, re-absorbption and secretion mechanisms, renal clearances. Digestive system: composition, production and role of digestive secretes, intestinal absorbption, liver function. Endocrine system: physiologic functions of hormones released by hypothalamus, pituitary gland, thyroid, parathyroid glands, surrenal glands, endocrine pancreas and gonades. Nervous system: the neurons
Expected learning objectivesThe student should: (KNOWLEDGE) -be aware of basic physiologic mechanisms that regulate the function of organs (COMPETENCE) -use the knowledge for solving main biotechnologic issues (TRANSVERSAL ABILITY) -to be able to read, understand and discuss technical material taken from books, other tests etc; -to know or find out the international terminology
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CourseLaboratory of cell and tissue analisys
Course IDMS0743
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMERLIN Simone
TeachersMERLIN Simone, BORSOTTI Chiara, BOLDORINI Renzo Luciano
CFU6
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year2
PeriodSecondo Semestre
Grading typeFinal grade
Modules
Course ID Course SSD Teachers
MS0445 Laboratory cell cultures BIO/17 - ISTOLOGIA MERLIN Simone, BORSOTTI Chiara
MS0230 Histopathological preparations MED/08 - ANATOMIA PATOLOGICA BOLDORINI Renzo Luciano
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CourseLaboratory cell cultures
Course IDMS0445
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMERLIN Simone
TeachersMERLIN Simone, BORSOTTI Chiara
CFU5
Teaching duration (hours)10
Individual study time 75
SSDBIO/17 - ISTOLOGIA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryA - Base
Year2
PeriodSecondo Semestre
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseLaboratory cell cultures
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMERLIN Simone
TeachersMERLIN Simone
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
Abstract- Safety in cell culture laboratory - Instruments and furniture in a cell culture laboratory - Aseptic techniques - Cell types and their characteristics - Cell culture media and supplements - Cell culture maintenance - Cell culture protocols: passaging, freezing, thawing - Cell counts with emocytometer - Contamination test - Practical experience in a cell culture laboratory
Reference textsTeacher slides presented in class
Teaching targetsKnowledge of the basic protocols for the correct maintenance of cell lines growing in suspension and in adhesion.
PrerequisitesFor the access to the practical laboratory it is necessary to pass the planned block exams. Knowledge of basic calculation methods used in chemical / biological laboratories
Didattics MethodsLectures in the classroom and practical exercises in the laboratory
Grading rules- Written test (multiple choices) - Assay on the practical cell laboratory experience
Full argumentsFrontal lessons on structure and organization of cell culture laboratories, biological risk, sterilization / disinfection methods; Practical lessons in the laboratory with cells growing in adhesion and suspension, freezing and thawing of cells, cell counts, and setting up a growth curve.
Expected learning objectivesAt the end of the module, the student knows and knows how to achieve: - the correct procedures for working in sterile conditions and the main basic techniques for animal cell cultures - a protocol to evaluate cell growth in different conditions - can critically evaluate the results obtained through the drafting, under the guidance of the teacher and tutors, of a report concerning the experiments carried out
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CourseLaboratory cell cultures
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBORSOTTI Chiara
TeachersBORSOTTI Chiara
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
Abstract- Safety in cell culture laboratory - Instruments and furniture in a cell culture laboratory - Aseptic techniques - Cell types and their characteristics - Cell culture media and supplements - Cell culture maintenance - Cell culture protocols: passaging, freezing, thawing - Cell counts with emocytometer - Contamination test - Practical experience in a cell culture laboratory
Reference textsTeacher slides presented in class
Teaching targetsKnowledge of the basic protocols for the correct maintenance of cell lines growing in suspension and in adhesion.
PrerequisitesFor the access to the practical laboratory it is necessary to pass the planned block exams. Knowledge of basic calculation methods used in chemical / biological laboratories
Didattics MethodsLectures in the classroom and practical exercises in the laboratory
Grading rules- Written test (multiple choices) - Assay on the practical cell laboratory experience
Full argumentsFrontal lessons on structure and organization of cell culture laboratories, biological risk, sterilization / disinfection methods; Practical lessons in the laboratory with cells growing in adhesion and suspension, freezing and thawing of cells, cell counts, and setting up a growth curve.
Expected learning objectivesAt the end of the module, the student knows and knows how to achieve: - the correct procedures for working in sterile conditions and the main basic techniques for animal cell cultures - a protocol to evaluate cell growth in different conditions - can critically evaluate the results obtained through the drafting, under the guidance of the teacher and tutors, of a report concerning the experiments carried out
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CourseHistopathological preparations
Course IDMS0230
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBOLDORINI Renzo Luciano
TeachersBOLDORINI Renzo Luciano
CFU1
Teaching duration (hours)8
Individual study time 13
SSDMED/08 - ANATOMIA PATOLOGICA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year2
PeriodSecondo Semestre
Sites and/or partitions
Gruppo A
Gruppo B
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CourseHistopathological preparations
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBOLDORINI Renzo Luciano
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
AbstractSpecifiche modalità di trattamento e fissazione dei materiali biologici, cito ed istologici significato dell’esame intraoperatorio. Principi e tecniche di estrazione degli acidi nucleici, metodiche di amplificazione genica, tecniche e principi di analisi di sequenza genica. Metodiche di immunoistochimica e di ibridazione in situ fluorescente.
Reference textsKumar, Abbas, Fausto. Robbins and Cotran Pathologic Basis of Disease. VII ed Elsevier, 2005-09-07 (anche ed italiana) Gallo, D'Amati: anatomia Patologica UTET
Teaching targetsLo studente dovrà essere in grado di comprendere l’importanza delle varie metodiche anatomopatologiche (citologia, istologia istoenzimatica e biologia molecolare)
PrerequisitesNozioni base di Anatomia Patologica, Anatomia ed Istologia e di Biologia Molecolare.
Didattics MethodsPresentazioni in formato power-point, CD-ROM interattivi e siti internet didattici.
Grading rulesEsame scritto con test a risposta multipla
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CourseHistopathological preparations
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersBOLDORINI Renzo Luciano
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
AbstractSpecifiche modalità di trattamento dei materiali biologici, cito ed istologici, significato dell’esame intraoperatorio. Principi e tecniche di estrazione degli acidi nucleici, metodiche di amplificazione genica, tecniche e principi di analisi di sequenza genica. Principi di colorazioni istochimiche ed immuno-istochimiche e loro utilizzo in ambito diagnostico.
Reference textsGallo P. - Anatomia Patologica per lauree triennali UTET
Teaching targetsConoscere i principi e le principali metodologie tecniche nella diagnostica autoptica, istologica e citologica in Anatomia Patologica. Comprendere le specifiche tecniche del trattameto degli acidi nucleici nella peculiarità di un laboratorio di Anatomia Patologica.
PrerequisitesConoscenze di base di chimica, anatomia ed istologia
Didattics MethodsPresentazioni in formato power-point, CD-ROM interattivi e siti internet didattici.
Grading rulesTest scritto a domande aperte o quiz a risposta multipla.
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CourseMolecular Biology
Course IDMS0740
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPELICCI Giuliana
TeachersCORA' Davide, PELICCI Giuliana
CFU6
Teaching duration (hours)48
Individual study time 102
SSDBIO/11 - BIOLOGIA MOLECOLARE
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodPrimo Semestre
Grading typeFinal grade
Sites and/or partitions
Gruppo B
Gruppo A
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CourseMolecular Biology
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCORA' Davide
TeachersCORA' Davide
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
Abstract- Introduction to molecular biology - Epigenetics and molecular biology: chromatin structure and regulation - Regulation of gene expression: transcription - mRNA processing and post-transcriptional regulation - microRNAs and Short-interferingRNAs - DNA repair - DNA replication - Cell cycle regulation - Cell response to stress - Apoptosis - Genetic manipulations in mice
Reference texts- Alberts et al. “Molecular biology of the Cell” VI E - Watson et al. "Molecular biology of the gene" VII E - Lewin “Genes X”
Teaching targetsProvide basic knowledge of molecular biology with a focus for eukaryotic organisms and with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.
Didattics MethodsPowerPoint presentations of lessons and articles provided by the lecturer.
Grading rulesMultiple-choice written exam.
Full argumentsOutline - Introduction to molecular biology. DNA structure, role of DNA as genetic material. - Genomics, complexity and gene regulation. - Comparative genomics. Homologous, paralogous and orthologous genes. - Gene Families (e.g. globin, tubulin); evolution and gene duplication. Molecular basis of epigenetics: chromatin structure and regulation. - structure of nucleosomes and chromatin organization. - Histones and their modifications (acetylation, methylation). - Mechanisms of histone remodeling and chromatin modifications and their role in the regulation of gene expression. Bromodomains and chromodomains. Role and examples of histone modifier enzymes: histone acetyl transferase (HAT), histone deacetylase. Histone remodeling complexes. - Insulators - DNA metilation: biological significance, DNA metil-transferase, the role of DNA methylation in regulating gene expression. Gene expression regulation: transcription - differences between transcription in prokaryotes and eukaryotes Transcription and gene expression regulation in eukaryotes: RNA polymerase II, promoter structure, basal transcription factors, Pol II and formation of the initiation complex. The role of the transcription initiation complex. - Recognition of transcription start sites: TATA box the transcription initiation complexes. - Transcription regulatory sequences and transcription factors (modular and dimeric organization of transcription factors). - Interactions between transcription factors and chromatin and remodeling complexes. - Mechanisms for transcription repression - Regulatory transcription activator strategies. Examples (NF-kB) - The main four structural classes of transcription factors: helix-loop-helix, helix-turn-helix (Myc / MAx / Mad), leucine zipper (Jun-Fos, AP1), zinc fingers (steroid hormone receptors). For each class: structural elements and interaction mechanisms with DNA; functional regulation; functions and regulated genes. RNA processing and post-transcriptional control - Meaning of capping and poly-adenylation of transcripts. Capping mechanisms polyadenylation and termination of mRNA. - The meaning of splicing. - Spliceosome and molecular mechanisms of splicing. Alternative splicing. - Splicing site recognition elements: ESE / ISE and ESS / ISS sequences. The role of SR proteins (containing RRS domains) and hRNPs proteins in splicing regulation. - Examples of pathologies caused by mutations affecting splicing. - mRNA editing mechanisms. - Regulation of mRNA transport and localization. - RNA stability (the IRE sequences for the control of stability and translation for mRNAs involved in the Transferrin and Ferritin Receptor). - MicroRNA: genomic structure, transcription and processing, the Dicer complex. - RISC complex, microRNA-mediated mechanisms for regulating gene expression (mRNA stability and translation regulation). Combinatorial nature of the interactions between microRNA and target genes. Examples. - Impact of microRNA discovery in understanding gene functions. Involvement in tumors. Clinical perspectives. - Short-interfering RNAs; basic research usage and clinical implications. DNA Repair Systems - DNA damage repair by base excision, repair of DNA replication errors, repair of DNA breaks on both filaments. DNA Replication - replication mechanism in eukaryotes. Telomeres and telomeres replication. Mechanism controlling cell cycle replication. Cell Cycle Regulation - General principles of cell cycle control. - Role of cyclin / Cdk complexes in cell cycle progression. - Molecular mechanisms of Cdk regulation: interaction with cyclins, activating phosphorylation and inhibiion, interaction with inhibitory proteins (p21, p16, p27 etc.). Mechanisms of regulation of cyclines: transcription, ubiquitination / degradation. - The role of the various cyclin / cdk complexes in the progression of the different phases of the cycle and the concept of "Checkpoint". - Regulation of cyclin / cdk complexes in G1 and S phase: activation of Jun / Fos and Myc and of Myc target genes; regulation of E2F1 by Cyclin D / Cdk4, Rb and cyclin E / Cdk2; Regulation of the cell cycle by tumor suppressors: the p21 family, p16, p27. The regulatory protein p53. Cellular response to stress - The response to DNA damage: transducers and effector complexes: Atm, ChK1 / 2, Cdc25 and p53 in the signal transduction cascade activated by DNA damage. - p53: structure, regulation (from Mdm2, Arf and phosphorylation via Atm / Chk) and function in response DNA damage, cellular senescence, and hypoxia response. Role and mechanisms through p53 promotes cell cycle arrest and apoptosis. Meaning of p53 mutations in tumors. - Cell transformation, general concepts for oncogenesis. Oncogenes and Proto-Oncogenes, fefinition and function. Mechanisms of activation of oncogenes (gain of function). Some examples of oncogenes (tyrosine kinases receptors, cytoplasmic kinase tyrosine, src, bcr-abl, RAS). Cancer suppressors (loss of function); loss of heterozygosity. Definition of caretaker and gatekeeper. Rb; cell cycle inhibitors; p53. - The role of apoptosis in cellular homeostasis. - Extrinsic way for apoptosis activation: pro-apoptotic receptors, death domain (DD) and death effector domain (DED) and the transduction of the apoptotic signal up to the activation of caspases regulators (caspase 8). - Caspase activation mechanism (caspase 3) and the role of their substrates in determining apoptosis (shape change, fragmentation of DNA, phosphoididylserine) and recognition of cells with phagocytic activity. - Intrinsic way for apoptosis activation: central role of regulation of permeability of external mitochondrial membrane in determining the release of cytochrome C, Smac / Diablo and other proteins which regulate apoptosis. Activation of caspases 9 by cytochrome c. Role for the IAP proteins (caspase inhibitors) in apoptosis regulation. - Different regulatory mechanisms for the 3 major caspase families (caspases 8, 9 and 3) - External mitochondrial membrane pore formation: pro-apoptotic proteins Bcl2, proteins Bcl2 and anti-apoptotic one. Functional Meaning of Domains BH1, BH2e BH3. - Necrosis: distinctive features from apoptosis Genetic Manipulations in the Mouse - Standard transgenesis, gene targeting, Cre-Lox systems (constituent vs inducible systems). Examples of mice transgenic or knock-out genes for the study of pathologies (Alzheimer's disease, p53 - / -).
Expected learning objectivesThe student must know the molecular and cellular mechanisms regulating replication, cell growth and the gene expression program in eukaryotic organisms with parallels in prokaryotic organisms.
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CourseMolecular Biology
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPELICCI Giuliana
TeachersPELICCI Giuliana
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
Abstract- Introduction to molecular biology - Epigenetics and molecular biology: chromatin structure and regulation - Regulation of gene expression: transcription - mRNA processing and post-transcriptional regulation - microRNAs and Short-interferingRNAs - DNA repair - DNA replication - Cell cycle regulation - Cell response to stress - Apoptosis - Genetic manipulations in mice
Reference textsAlberts et al. “Molecular biology of the Cell” VI E Watson et al. "Molecular biology of the gene" VII E Lewin “Genes X”
Teaching targetsProvide basic knowledge of molecular biology with a focus for eukaryotic organisms and with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.
PrerequisitesGeneral knowledge on cellular biology
Didattics MethodsPowerPoint presentations of lessons and articles provided by the lecturer
Other informationsnone
Grading rulesMultiple-choice written exam
Full argumentsOutline - Introduction to molecular biology. DNA structure, role of DNA as genetic material. - Genomics, complexity and gene regulation. - Comparative genomics. Homologous, paralogous and orthologous genes. - Gene Families (e.g. globin, tubulin); evolution and gene duplication. Molecular basis of epigenetics: chromatin structure and regulation. - structure of nucleosomes and chromatin organization. - Histones and their modifications (acetylation, methylation). - Mechanisms of histone remodeling and chromatin modifications and their role in the regulation of gene expression. Bromodomains and chromodomains. Role and examples of histone modifier enzymes: histone acetyl transferase (HAT), histone deacetylase. Histone remodeling complexes. - Insulators - DNA metilation: biological significance, DNA metil-transferase, the role of DNA methylation in regulating gene expression. Gene expression regulation: transcription - differences between transcription in prokaryotes and eukaryotes Transcription and gene expression regulation in eukaryotes: RNA polymerase II, promoter structure, basal transcription factors, Pol II and formation of the initiation complex. The role of the transcription initiation complex. - Recognition of transcription start sites: TATA box the transcription initiation complexes. - Transcription regulatory sequences and transcription factors (modular and dimeric organization of transcription factors). - Interactions between transcription factors and chromatin and remodeling complexes. - Mechanisms for transcription repression - Regulatory transcription activator strategies. Examples (NF-kB) - The main four structural classes of transcription factors: helix-loop-helix, helix-turn-helix (Myc / MAx / Mad), leucine zipper (Jun-Fos, AP1), zinc fingers (steroid hormone receptors). For each class: structural elements and interaction mechanisms with DNA; functional regulation; functions and regulated genes. RNA processing and post-transcriptional control - Meaning of capping and poly-adenylation of transcripts. Capping mechanisms polyadenylation and termination of mRNA. - The meaning of splicing. - Spliceosome and molecular mechanisms of splicing. Alternative splicing. - Splicing site recognition elements: ESE / ISE and ESS / ISS sequences. The role of SR proteins (containing RRS domains) and hRNPs proteins in splicing regulation. - Examples of pathologies caused by mutations affecting splicing. - mRNA editing mechanisms. - Regulation of mRNA transport and localization. - RNA stability (the IRE sequences for the control of stability and translation for mRNAs involved in the Transferrin and Ferritin Receptor). - MicroRNA: genomic structure, transcription and processing, the Dicer complex. - RISC complex, microRNA-mediated mechanisms for regulating gene expression (mRNA stability and translation regulation). Combinatorial nature of the interactions between microRNA and target genes. Examples. - Impact of microRNA discovery in understanding gene functions. Involvement in tumors. Clinical perspectives. - Short-interfering RNAs; basic research usage and clinical implications. DNA Repair Systems - DNA damage repair by base excision, repair of DNA replication errors, repair of DNA breaks on both filaments. DNA Replication - replication mechanism in eukaryotes. Telomeres and telomeres replication. Mechanism controlling cell cycle replication. Cell Cycle Regulation - General principles of cell cycle control. - Role of cyclin / Cdk complexes in cell cycle progression. - Molecular mechanisms of Cdk regulation: interaction with cyclins, activating phosphorylation and inhibiion, interaction with inhibitory proteins (p21, p16, p27 etc.). Mechanisms of regulation of cyclines: transcription, ubiquitination / degradation. - The role of the various cyclin / cdk complexes in the progression of the different phases of the cycle and the concept of "Checkpoint". - Regulation of cyclin / cdk complexes in G1 and S phase: activation of Jun / Fos and Myc and of Myc target genes; regulation of E2F1 by Cyclin D / Cdk4, Rb and cyclin E / Cdk2; Regulation of the cell cycle by tumor suppressors: the p21 family, p16, p27. The regulatory protein p53. Cellular response to stress - The response to DNA damage: transducers and effector complexes: Atm, ChK1 / 2, Cdc25 and p53 in the signal transduction cascade activated by DNA damage. - p53: structure, regulation (from Mdm2, Arf and phosphorylation via Atm / Chk) and function in response DNA damage, cellular senescence, and hypoxia response. Role and mechanisms through p53 promotes cell cycle arrest and apoptosis. Meaning of p53 mutations in tumors. - Cell transformation, general concepts for oncogenesis. Oncogenes and Proto-Oncogenes, fefinition and function. Mechanisms of activation of oncogenes (gain of function). Some examples of oncogenes (tyrosine kinases receptors, cytoplasmic kinase tyrosine, src, bcr-abl, RAS). Cancer suppressors (loss of function); loss of heterozygosity. Definition of caretaker and gatekeeper. Rb; cell cycle inhibitors; p53. - The role of apoptosis in cellular homeostasis. - Extrinsic way for apoptosis activation: pro-apoptotic receptors, death domain (DD) and death effector domain (DED) and the transduction of the apoptotic signal up to the activation of caspases regulators (caspase 8). - Caspase activation mechanism (caspase 3) and the role of their substrates in determining apoptosis (shape change, fragmentation of DNA, phosphoididylserine) and recognition of cells with phagocytic activity. - Intrinsic way for apoptosis activation: central role of regulation of permeability of external mitochondrial membrane in determining the release of cytochrome C, Smac / Diablo and other proteins which regulate apoptosis. Activation of caspases 9 by cytochrome c. Role for the IAP proteins (caspase inhibitors) in apoptosis regulation. - Different regulatory mechanisms for the 3 major caspase families (caspases 8, 9 and 3) - External mitochondrial membrane pore formation: pro-apoptotic proteins Bcl2, proteins Bcl2 and anti-apoptotic one. Functional Meaning of Domains BH1, BH2e BH3. - Necrosis: distinctive features from apoptosis Genetic Manipulations in the Mouse - Standard transgenesis, gene targeting, Cre-Lox systems (constituent vs inducible systems). Examples of mice transgenic or knock-out genes for the study of pathologies (Alzheimer's disease, p53 - / -)
Expected learning objectivesThe student must know the molecular and cellular mechanisms regulating replication, cell growth and the gene expression program in eukaryotic organisms with parallels in prokaryotic organisms.
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CourseMolecular technology laboratory
Course IDMS0741
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderSANTORO Claudio Ventura
TeachersSANTORO Claudio Ventura, CORAZZARI Marco, GIORDANO Mara, CORRADO Lucia
CFU7
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year2
PeriodPrimo Semestre
Grading typeFinal grade
Teaching languageitalian
Abstractthose of each module
Reference textsthose of each module
Teaching targetsthose of each module
Prerequisitesthose of each module
Didattics Methodsthose of each module
Other informationson web site DIR
Grading rulesthose of each module
Full argumentsthose of each module
Expected learning objectivesthose of each module
Modules
Course ID Course SSD Teachers
BT023 Recombinant technology lab BIO/13 - BIOLOGIA APPLICATA SANTORO Claudio Ventura, CORAZZARI Marco
MS0742 Genetics laboratory MED/03 - GENETICA MEDICA GIORDANO Mara, CORRADO Lucia
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CourseRecombinant technology lab
Course IDBT023
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderSANTORO Claudio Ventura
TeachersSANTORO Claudio Ventura, CORAZZARI Marco
CFU5
Teaching duration (hours)16
Individual study time 73
SSDBIO/13 - BIOLOGIA APPLICATA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year2
PeriodPrimo Semestre
Sites and/or partitions
Gruppo A
Gruppo B
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CourseRecombinant technology lab
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderSANTORO Claudio Ventura
TeachersSANTORO Claudio Ventura
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageitalian
Abstracttheoretical principles and some practical aspects of the main recombinant DNA techniques
Reference texts- James D. Watson et al. "DNA Ricombinante" - Zanichelli (2nd ed) - Jeremy Dale, et al. "Dai Geni ai Genomi" - EDISES (3rd ed) - Terry A. Brown "Biotecnologie Molecolari" - Zanichelli (2nd ed)
Teaching targetsProvide the theoretical and practical bases of the methods and technologies used in the study and analysis of gene expression, with particular emphasis on recombinant DNA techniques used to clone, express and analyze gene products of biomedical interest and applications. Importantly, the frequency of a dedicated laboratory is mandatory.
PrerequisitesThe student must know both cellular biology and basic genetics, and in particular the molecular and cellular mechanisms regulating replication, cell growth and gene expression programs in both prokaryotic and eukaryotic organisms.
Didattics Methodsclassroom and laboratory lessons
Other informationsavailable on DIR website
Grading ruleswritten test (multiple-choice questions)
Full argumentsCloning. Gene manipulation strategies. Key features of cloning vectors. Expression of recombinant proteins. Strategies to generate and maintain genetically modified organisms. Technologies used in genetic diagnosis. Cloning and analysis of genotypes. PCR: Principles and Uses. Targeted mutagenicity. Expression of recombinant proteins in E. coli. Methods of gene transformation. Expression of recombinant proteins in eukaryotic cells. Expression of transgenes in animal models. DNA arrays, Gene-chips. PTT, SNP. Proteomics. Genetic strategies to manipulate the phenotype of organisms of socio-economic interest. Animal & Plant Pharming.
Expected learning objectivesThe student will become familiar with the main methods and technologies used to study and analyze gene expression, together with the main techniques used to clone, express and analyze gene products of biomedical interest and application. Acquired familiarity with instrumentation and working methods of a research / analysis laboratory.
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CourseRecombinant technology lab
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCORAZZARI Marco
TeachersCORAZZARI Marco
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageitalian
Abstracttheoretical principles and some practical aspects of the main recombinant DNA techniques
Reference texts- James D. Watson et al. "DNA Ricombinante" - Zanichelli (2nd ed) - Jeremy Dale, et al. "Dai Geni ai Genomi" - EDISES (3rd ed) - Terry A. Brown "Biotecnologie Molecolari" - Zanichelli (2nd ed)
Teaching targetsProvide the theoretical and practical bases of the methods and technologies used in the study and analysis of gene expression, with particular emphasis on recombinant DNA techniques used to clone, express and analyze gene products of biomedical interest and applications. Importantly, the frequency of a dedicated laboratory is mandatory.
PrerequisitesThe student must know both cellular biology and basic genetics, and in particular the molecular and cellular mechanisms regulating replication, cell growth and gene expression programs in both prokaryotic and eukaryotic organisms.
Didattics Methodsclassroom and laboratory lessons
Other informationsavailable on DIR website
Grading ruleswritten test (multiple-choice questions)
Full argumentsCloning. Gene manipulation strategies. Key features of cloning vectors. Expression of recombinant proteins. Strategies to generate and maintain genetically modified organisms. Technologies used in genetic diagnosis. Cloning and analysis of genotypes. PCR: Principles and Uses. Targeted mutagenicity. Expression of recombinant proteins in E. coli. Methods of gene transformation. Expression of recombinant proteins in eukaryotic cells. Expression of transgenes in animal models. DNA arrays, Gene-chips. PTT, SNP. Proteomics. Genetic strategies to manipulate the phenotype of organisms of socio-economic interest. Animal & Plant Pharming.
Expected learning objectivesThe student will become familiar with the main methods and technologies used to study and analyze gene expression, together with the main techniques used to clone, express and analyze gene products of biomedical interest and application. Acquired familiarity with instrumentation and working methods of a research / analysis laboratory.
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CourseGenetics laboratory
Course IDMS0742
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderGIORDANO Mara
TeachersGIORDANO Mara, CORRADO Lucia
CFU2
Individual study time 26
SSDMED/03 - GENETICA MEDICA
Course typeModulo di sola Frequenza
Course categoryC - Affine o integrativo
Year2
PeriodPrimo Semestre
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseGenetics laboratory
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersGIORDANO Mara
Course typeGruppi
Year2
PartitionGruppo A
Teaching languageItalian
AbstractMethods of molecular genetics Genomic DNA purification, DNA amplification and analysis ( fragment analysis and sequencing)
Reference textshandouts with protocols distributed by the teacher in the laboratory
Teaching targetsTo apply the basic methods of molecular genetics such as PCR and sequencing through practical laboratory activities
PrerequisitesFundamentals of genetics. DNA analysis techniques
Didattics Methodspractical laboratory activity aimed at performing genetic analysis
Grading rulesThe knowledge will be verified by written exam with open and / or multiple choice questions.
Full argumentsDNA investigation methods. Polymerase Chain Reaction (PCR); Analysis of VNTR, STR and fingerprint polymorphisms; DNA sequencing; Methods for the identification of nucleotide variations. Genomic analysis through next generation
Expected learning objectivesApply knowledge to the analysis and resolution of problems of formal and molecular genetics.
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CourseGenetics laboratory
Academic Year2018/2019
Year of rule2017/2018
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersCORRADO Lucia
Course typeGruppi
Year2
PartitionGruppo B
Teaching languageItalian
AbstractMethods of molecular genetics Genomic DNA purification, DNA amplification and analysis ( fragment analysis and sequencing)
Reference textshandouts with protocols distributed by the teacher in the laboratory
Teaching targetsTo apply the basic methods of molecular genetics such as PCR and sequencing through practical laboratory activities
PrerequisitesFundamentals of genetics. DNA analysis techniques
Didattics Methodspractical laboratory activity aimed at performing genetic analysis
Grading rulesThe knowledge will be verified by written exam with open and / or multiple choice questions.
Full argumentsDNA investigation methods. Polymerase Chain Reaction (PCR); Analysis of VNTR, STR and fingerprint polymorphisms; DNA sequencing; Methods for the identification of nucleotide variations. Genomic analysis through next generation
Expected learning objectivesApply knowledge to the analysis and resolution of problems of formal and molecular genetics.
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CourseBiotechnological applications in the clinical laboratory
Course IDMS0371
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderROLLA Roberta
TeachersCRISA' Elena, MARGIOTTA CASALUCI Gloria, ROLLA Roberta
CFU10
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year3
PeriodSecondo Semestre
Grading typeFinal grade
Teaching languageitalian
AbstractThe course describes the biotechnology used in Hematology and Clinical Pathology.
Reference textsTeaching Material provided by the lecturer. Lecturer's Monographs.
Teaching targetsProvide a specific knowledge of biotechnology used in Hematology and Clinical Pathology.
PrerequisitesGood knowledge of Chemistry, Biochemistry, Molecular Biology and Genetics.
Didattics MethodsPowePoint presentation. Teaching Network on DIR Website. Frontal lessons. Laboratory exercises.
Other informationsLaboratory exercises at Clinical Chemistry Unit, "Maggiore della Carità" Hospital, Novara, during which will be deepened the topics, previously discussed during the lessons.
Grading rulesMultiple choice test.
Full arguments80 hours of lectures, in which are described the topics listed below. Clinical and molecular diagnostics of leukemia and lymphomas. Molecular Diagnostics in Clinical Pathology, in particular the following molecular biology techniques are described: • RealTime PCR (FRET probes) • Hybridization with ASO (Allele-Specific Oligonucleotide) • Microarray • Pyrosequencing • Mass spectrometry for nucleic acids analysis. Then are evaluated the molecular biology tests used in the diagnosis of the following diseases: • Cystic Fibrosis • Y chromosome microdeletion • Hereditary thrombophilia • Hemochromatosis In particular, the pathophysiological, biochemical and molecular bases of these pathologies are described. Description of Biotechnology used in Clinical Pathology for the study of hemostasis and coagulation. In particular: • biotechnologies used in the evaluation of coagulation: thrombotic risk and bleeding risk (screening tests and specialized tests). • biotechnologies used in the evaluation of platelet function (reticulated platelets, PFA, Multiplate, aggregometry). Description of Biotechnology used in Clinical Pathology for the study of blood diseases (red line and white line) and for the evaluation of body fluids (cerebrospinal fluid, ascites, pleural fluid, synovial fluid, cardiac liquid). In particular: • blood count • biochemical parameters for the evaluation of anemia • flow cytometry for leukaemias phenotyping • ion exchange chromatography for the evaluation of hemoglobinopathies and thalassemia. In particular are described the pathophysiological, biochemical and molecular bases of α and β thalassemia. D. Electrolyte, and Acid Base Balance E. Electrophoresis of human plasma proteins F. Biochemical markers of myocardial injury G. Renin-angiotensin-aldosterone system H. The clinical use of thyroid function tests I. Lipoprotein metabolism J. Pathophysiology of atherosclerosis
Modules
Course ID Course SSD Teachers
BT011 Hematology MED/15 - MALATTIE DEL SANGUE CRISA' Elena, MARGIOTTA CASALUCI Gloria
BM047 Clinical pathology MED/05 - PATOLOGIA CLINICA ROLLA Roberta
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CourseHematology
Course IDBT011
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersCRISA' Elena, MARGIOTTA CASALUCI Gloria
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/15 - MALATTIE DEL SANGUE
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CourseHematology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCRISA' Elena
TeachersCRISA' Elena
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageItalian
Reference textsMateriale didattico fornito dal Docente sulla piattaforma DIR (Didattica in rete).
Teaching targets1. Conoscere ed interpretare la diagnostica morfologica delle malattie del sangue 2. Conoscere ed interpretare un esame emocromocitometrico 3. Conoscere ed interpretare i test diagnostici per le anemie ereditarie 4. Conoscere ed interpretare i test diagnostici per le alterazioni di conta e funzionalità piastrinica 5. Conoscere le applicazioni della citogenetica convenzionale e molecolare alla diagnostica e gestione delle malattie del sangue 6. Conoscere le applicazioni dei test molecolari alla diagnostica e gestione delle malattie del sangue 7. Conoscere le applicazioni dei test citofluorimetrici alla diagnostica e gestione delle malattie del sangue 8. Conoscere ed interpretare i test di medicina trasfusionale 9. Conoscere le applicazioni biotecnologiche al trapianto di cellule staminali emopoietiche 10. Conoscere le applicazioni alle malattie del sangue della genomica (gene expression profille, SNP array, GWAS, whole genome/exome sequencing)
PrerequisitesLo studente deve essere in possesso dei fondamenti della biologia cellulare, della immunologia, della citogenetica, della biologia molecolare, della istologia e citologia
Didattics Methods1.valutazione di immagini da microscopio ottico di uno striscio di sangue periferico 2. valutazione di immagini di preparati mediante FISH 3. Interpretazione di una sequenza Sanger per la identificazione di mutazioni 4. Interpretazione di una PCR quantitativa per il trascritto di fusione BCR/ABL 5. Presentazioni in formato MS-Power Point 6. siti internet didattici 7. Analisi e revisione di letteratura scientifica a piccoli gruppi con presentazione finale
Other informationsGli studenti hanno la possibilità di eseguire un internato presso il Laboratorio di Ematologia. Durante la frequenza presso il Laboratorio di Ematologia, lo studente sarà affiancato ad uno dei Biologi avviato ad alcune tecniche di diagnostica in Ematologia, con particolare riguardo alla diagnostica citomorfologica, alla diagnostica citogenetica ed alla diagnostica molecolare applicata alla oncoematologia
Grading rulesLa verifica dell’apprendimento avverrà tramite prova scritta con quiz a scelta multipla
Full argumentsDiagnostica in ematologia: 1. emocromo e test su sangue venoso periferico 2. biopsia osteo midollare e test su sangue midollare Morfologia delle cellule del sangue 1. Tecnica dello striscio di sangue periferico 2. Colorazione May Grunwald/Giemsa 3. Morfologia filologica e patologica dell'eritrocita 4. Morfologia fisiologica e patologica del leucocita 5. Morfologia fisiologica e patologica delle piastrine 6. Morfologia fisiologica e patologica dei precursori emopoietici La diagnostica citofluorimetrica applicata alle malattie del sangue 1. Diagnostica citofluorimetrica delle malattie del sangue 2. Analisi della malattia minima residua mediante citofluorimetria Applicazioni citogenetiche alle malattie del sangue 1. Principi del cariotipo convenzionale 2. Principi del cariotipo molecolare FISH 3. Le principali anomalie citogenetiche associate alle malattie del sangue La biologia molecolare delle malattie del sangue 1. Analisi del riarrangiamento delle immunoglobuline nella diagnostica delle malattie del sangue 2. Analisi del riarrangiamento del recettore T nella diagnostica delle malattie del sangue 3. Analisi mutazionale nella diagnostica delle malattie del sangue ereditarie ed acquisite 4. Ricerca dei trascritti di fusione nella diagnostica delle malattie del sangue 5. Analisi della malattia minima residua mediante approcci di biologia molecolare e RT-PCR 6. Analisi dell'attecchimento midollare dopo trapianto di cellule staminali emopoietiche allogeniche tramite Short Tandem Repeats Cenni di inquadramento diagnostico-terapeutico della 1. patologia mieloide 2. patologia linfoide 3. patologia globulo rosso 4. patologia piastrinica Test di medicina trasfusionale 1. I gruppi ematici 2. I test di compatibilità Trapianto di cellule staminali emopoietiche 1. Il sistema HLA 2. Raccolta e preparazione delle cellule staminali per il trapianto 3. Manipolazione del graft per il trapianto 4. Monitoraggio dell'attecchimento e della ricostituzione immunologica Genomica delle malattie del sangue 1. Gene expression profile 2. Genome wide association studies 3. SNP array 4. Whole genome and whole exome sequencing
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CourseHematology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMARGIOTTA CASALUCI Gloria
TeachersMARGIOTTA CASALUCI Gloria
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageItalian
Reference textsMateriale didattico fornito dal Docente sulla piattaforma DIR (Didattica in rete).
Teaching targets1. Conoscere ed interpretare la diagnostica morfologica delle malattie del sangue 2. Conoscere ed interpretare un esame emocromocitometrico 3. Conoscere ed interpretare i test diagnostici per le anemie ereditarie 4. Conoscere ed interpretare i test diagnostici per le alterazioni di conta e funzionalità piastrinica 5. Conoscere le applicazioni della citogenetica convenzionale e molecolare alla diagnostica e gestione delle malattie del sangue 6. Conoscere le applicazioni dei test molecolari alla diagnostica e gestione delle malattie del sangue 7. Conoscere le applicazioni dei test citofluorimetrici alla diagnostica e gestione delle malattie del sangue 8. Conoscere ed interpretare i test di medicina trasfusionale 9. Conoscere le applicazioni biotecnologiche al trapianto di cellule staminali emopoietiche 10. Conoscere le applicazioni alle malattie del sangue della genomica (gene expression profille, SNP array, GWAS, whole genome/exome sequencing)
PrerequisitesLo studente deve essere in possesso dei fondamenti della biologia cellulare, della immunologia, della citogenetica, della biologia molecolare, della istologia e citologia.
Didattics Methods1. Valutazione di immagini da microscopio ottico di uno striscio di sangue periferico 2. Valutazione di immagini di preparati mediante FISH 3. Interpretazione di una sequenza Sanger per la identificazione di mutazioni 4. Interpretazione di una PCR quantitativa per il trascritto di fusione BCR/ABL 5. Presentazioni in formato MS-Power Point 6. Siti internet didattici 7. Analisi e revisione di letteratura scientifica a piccoli gruppi con presentazione finale
Other informationsGli studenti hanno la possibilità di eseguire un internato presso il Laboratorio di Ematologia. Durante la frequenza presso il Laboratorio di Ematologia, lo studente sarà affiancato ad uno dei Biologi avviato ad alcune tecniche di diagnostica in Ematologia, con particolare riguardo alla diagnostica citomorfologica, alla diagnostica citogenetica ed alla diagnostica molecolare applicata alla oncoematologia.
Grading rulesLa verifica dell’apprendimento avverrà tramite prova scritta con quiz a scelta multipla.
Full argumentsDiagnostica in ematologia: 1. Emocromo e test su sangue venoso periferico 2. Biopsia osteo-midollare e test su sangue midollare Morfologia delle cellule del sangue: 1. Tecnica dello striscio di sangue periferico 2. Colorazione May Grunwald/Giemsa 3. Morfologia fisiologica e patologica dell'eritrocita 4. Morfologia fisiologica e patologica del leucocita 5. Morfologia fisiologica e patologica delle piastrine 6. Morfologia fisiologica e patologica dei precursori emopoietici La diagnostica citofluorimetrica applicata alle malattie del sangue: 1. Diagnostica citofluorimetrica delle malattie del sangue 2. Analisi della malattia minima residua mediante citofluorimetria Applicazioni citogenetiche alle malattie del sangue: 1. Principi del cariotipo convenzionale 2. Principi del cariotipo molecolare FISH 3. Le principali anomalie citogenetiche associate alle malattie del sangue La biologia molecolare delle malattie del sangue: 1. Analisi del riarrangiamento delle immunoglobuline nella diagnostica delle malattie del sangue 2. Analisi del riarrangiamento del recettore T nella diagnostica delle malattie del sangue 3. Analisi mutazionale nella diagnostica delle malattie del sangue ereditarie ed acquisite 4. Ricerca dei trascritti di fusione nella diagnostica delle malattie del sangue 5. Analisi della malattia minima residua mediante approcci di biologia molecolare e RT-PCR 6. Analisi dell'attecchimento midollare dopo trapianto di cellule staminali emopoietiche allogeniche tramite Short Tandem Repeats Cenni di inquadramento diagnostico-terapeutico della: 1. patologia mieloide 2. patologia linfoide 3. patologia del globulo rosso 4. patologia piastrinica Test di medicina trasfusionale: 1. I gruppi ematici 2. I test di compatibilità Trapianto di cellule staminali emopoietiche: 1. Il sistema HLA 2. Raccolta e preparazione delle cellule staminali per il trapianto 3. Manipolazione del graft per il trapianto 4. Monitoraggio dell'attecchimento e della ricostituzione immunologica Genomica delle malattie del sangue: 1. Gene expression profile 2. Genome wide association studies 3. SNP array 4. Whole genome and whole exome sequencing
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CourseClinical pathology
Course IDBM047
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderROLLA Roberta
TeachersROLLA Roberta
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/05 - PATOLOGIA CLINICA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CourseClinical pathology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderROLLA Roberta
TeachersROLLA Roberta
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageItalian
AbstractThe course describes the biotechnology used in Clinical Pathology.
Reference textsTeaching Material provided by the lecturer. Lecturer's Monographs.
Teaching targetsProvide a specific knowledge of biotechnology used in Clinical Pathology.
PrerequisitesGood knowledge of Chemistry, Biochemistry, Molecular Biology and Genetics.
Didattics MethodsPowePoint presentation. Teaching Network on DIR Website. Frontal lessons. Laboratory exercises.
Other informationsLaboratory exercises at Clinical Chemistry Unit, "Maggiore della Carità" Hospital, Novara, during which will be deepened the topics, previously discussed during the lessons.
Grading rulesMultiple choice test.
Full arguments40 hours of lectures, in which are described the topics listed below. A. Molecular Diagnostics in Clinical Pathology, in particular the following molecular biology techniques are described: • RealTime PCR (FRET probes) • Hybridization with ASO (Allele-Specific Oligonucleotide) • Microarray • Pyrosequencing • Mass spectrometry for nucleic acids analysis. Then are evaluated the molecular biology tests used in the diagnosis of the following diseases: • Cystic Fibrosis • Y chromosome microdeletion • Hereditary thrombophilia • Hemochromatosis In particular, the pathophysiological, biochemical and molecular bases of these pathologies are described. B. Description of Biotechnology used in Clinical Pathology for the study of hemostasis and coagulation. In particular: • biotechnologies used in the evaluation of coagulation: thrombotic risk and bleeding risk (screening tests and specialized tests). • biotechnologies used in the evaluation of platelet function (reticulated platelets, PFA, Multiplate, aggregometry). C. Description of Biotechnology used in Clinical Pathology for the study of blood diseases (red line and white line) and for the evaluation of body fluids (cerebrospinal fluid, ascites, pleural fluid, synovial fluid, cardiac liquid). In particular: • blood count • biochemical parameters for the evaluation of anemia • flow cytometry for leukaemias phenotyping • ion exchange chromatography for the evaluation of hemoglobinopathies and thalassemia. In particular are described the pathophysiological, biochemical and molecular bases of α and β thalassemia. D. Electrolyte, and Acid Base Balance E. Electrophoresis of human plasma proteins F. Biochemical markers of myocardial injury G. Renin-angiotensin-aldosterone system H. The clinical use of thyroid function tests I. Lipoprotein metabolism J. Pathophysiology of atherosclerosis
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CourseClinical pathology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderROLLA Roberta
TeachersROLLA Roberta
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageItalian
AbstractThe course describes the biotechnology used in Clinical Pathology.
Reference textsTeaching Material provided by the lecturer. Lecturer's Monographs.
Teaching targetsProvide a specific knowledge of biotechnology used in Clinical Pathology.
PrerequisitesGood knowledge of Chemistry, Biochemistry, Molecular Biology and Genetics.
Didattics MethodsPowePoint presentation. Teaching Network on DIR Website. Frontal lessons. Laboratory exercises.
Other informationsLaboratory exercises at Clinical Chemistry Unit, "Maggiore della Carità" Hospital, Novara, during which will be deepened the topics, previously discussed during the lessons.
Grading rulesMultiple choice test.
Full arguments40 hours of lectures, in which are described the topics listed below. A. Molecular Diagnostics in Clinical Pathology, in particular the following molecular biology techniques are described: • RealTime PCR (FRET probes) • Hybridization with ASO (Allele-Specific Oligonucleotide) • Microarray • Pyrosequencing • Mass spectrometry for nucleic acids analysis. Then are evaluated the molecular biology tests used in the diagnosis of the following diseases: • Cystic Fibrosis • Y chromosome microdeletion • Hereditary thrombophilia • Hemochromatosis In particular, the pathophysiological, biochemical and molecular bases of these pathologies are described. B. Description of Biotechnology used in Clinical Pathology for the study of hemostasis and coagulation. In particular: • biotechnologies used in the evaluation of coagulation: thrombotic risk and bleeding risk (screening tests and specialized tests). • biotechnologies used in the evaluation of platelet function (reticulated platelets, PFA, Multiplate, aggregometry). C. Description of Biotechnology used in Clinical Pathology for the study of blood diseases (red line and white line) and for the evaluation of body fluids (cerebrospinal fluid, ascites, pleural fluid, synovial fluid, cardiac liquid). In particular: • blood count • biochemical parameters for the evaluation of anemia • flow cytometry for leukaemias phenotyping • ion exchange chromatography for the evaluation of hemoglobinopathies and thalassemia. In particular are described the pathophysiological, biochemical and molecular bases of α and β thalassemia. D. Electrolyte, and Acid Base Balance E. Electrophoresis of human plasma proteins F. Biochemical markers of myocardial injury G. Renin-angiotensin-aldosterone system H. The clinical use of thyroid function tests I. Lipoprotein metabolism J. Pathophysiology of atherosclerosis
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CourseClinical Biochemistry and Clinical Molecular Biology
Course IDMS0450
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCAPELLO Daniela
TeachersCAPELLO Daniela
CFU5
Teaching duration (hours)40
Individual study time 85
SSDBIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year3
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseClinical Biochemistry and Clinical Molecular Biology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCAPELLO Daniela
TeachersCAPELLO Daniela
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageItalian
Abstract- Proteomic, metabolomic and genomic biomarkers: definition and use. - Preanalytical, analytical and post-analytical factors that influence the laboratory result. Significance and limits of laboratory procedures and results: assessment of the quality and informative value of laboratory results. - Genomic markers and molecular tests: clinical applications of molecular biology. The main molecular biology protocols applied to diagnostics: chemical-physical principles and fields of application - Protein and enzymatic biomarkers used to investigate organ functionality and hereditary metabolic disorders - Main signal transduction pathways in normal cells and their alterations in metabolic disorders and neoplasia. - Representative genetic lesions in cancer employed as diagnostic, predictive and therapeutic markers
Reference textsAntonozzi-Gulletta Medicina di Laboratorio Logica e Patologia Clinica, Piccin
Teaching targetsUnderstanding how metabolic, protein and genetic markers can be used as diagnostic, predictive and therapeutic indicators. Understanding how biochemical and molecular biology techniques are used in diagnostics Understanding the informative value of the enzymatic and metabolic markers in the evaluation of organ functionality and in the diagnosis of metabolic disorders. Acquire the basic knowledge on the use of "genome wide" methodologies used in translational research, applied to the concept of "personalized medicine". Being able to use the theoretical and applicative knowledge of the different biochemical and molecular diagnostic methods as tools to conceive, optimize and apply specific diagnostic procedures in response to various clinical questions To gain an overview on the regulation of metabolic processes in different organs and tissues and to understand how single alterations in the intracellular signaling pathways can compromise the homeostasis not only of the cell or of the tissue, but of the whole organism.
PrerequisitesBasic knowledge on metabolic pathways and energetic metabolism, signal transtuction and regulation of gene expression
Didattics MethodsFrontal lessons with slides presentation. Execution of problems, quizzes and exposition of cases in the classroom to show precise molecular diagnostic problems and guide the student to their resolution. Running problems, quizzes and case resolution at home using the moodle platform. For the preparation of the exam students can use the material provided by the teacher (a pdf copy of the slides projected in class, of handouts prepared by the teacher and of some articles taken from scientific journals on the topics discussed during the course) and on the recommended textbook
Other informationsA pdf copy of the projected slides, the in-depth material and all the information regarding the course and the methods of examination will be made available on the DIR (https://www.dir.uniupo.it/)
Grading rulesThe aim of the exam consists in verifying the level of knowledge skills developed by the student concerning the course program topics. The exam will consist in a written test including 28 questions related to all the topics of the course program divided into: quizzes with multiple choice answers or true / false and 3 exercises that require the resolution of problems concerning the determination of characteristics of a laboratory test (sensitivity, specificity and predictive values). The evaluation is expressed in thirtieths (minimum mark 18) and will consist of a maximum of 28 points for questions with multiple choice answers (1 point for each question) and a maximum of 5 points for the exercises. During the written test it is not allowed to consult any kind of material. The calculator is allowed. The exam allows to evaluate the theoretical knowledge, the critical sense, the ability to understand the text (exercises with a request to make a choice between different alternatives) and the ability to apply the acquired knowledge to practical circumstances (numerical exercises).
Full arguments1. Introduction Biomarkers - Purpose and meaning of diagnostic tests - Proteomic, metabolomic and genomic biomarkers The pre-analytical, analytical and post-analytic steps in laboratory investigations - Variability of biological material and of pre-analytical procedures - Analytical phase: meaning, limits and properties of laboratory procedures. - Post-analytical phase: definition of reference values and threshold values. Diagnostic sensitivity and specificity; predictive values of a diagnostic test 2_ Genomic biomarkers: clinical applications of molecular biology. - Genetic alterations investigated in molecular diagnostics. Potential and limits of molecular tests - Qualitative and quantitative real-time PCR: principles and its application genetic lesions screening - From Sanger sequencing to IV generation sequencing - mRNA sequencing to identify novel molecular markers - Identification of structural DNA variations. Principles and applications in the diagnostics of oncological and congenital diseases 3_Clinical Clinic of organs and systems Protein biomarkers - Plasma proteins: role and quantitative variations - The reactive proteins of the acute phase: classification, regulation of their expression and role in diseases Enzymatic biomarkers - Selection criteria for enzymatic biomarkers in the clinical laboratory. Methods of determination of enzymatic activities - Enzymes used in the clinical laboratory: their physiological function and their use as disease markers. Inborn metabolic alterations - Investigation of the metabolome, of the proteome and the enzymatic dosages: applications and limits. - Definition of diagnostic screening and its applications. - Inborn metabolic alterations investigated by neonatal screening 4_Cell signaling - Functional classification of growth factors, hormones, cytokines and their receptors - Signalling associated with receptors coupled to heterotrimeric proteins G, membrane receptors with or without intrinsic enzymatic activity and intracellular receptors - MAP-kinases, JAK/STAT; PI3K/AKT/mTOR pathways. Small G-proteins family. -Signalling in hormonal regulation: hypothalamic-hypophyseal axis and its peripheral targets; cortical adrenal hormones; gonadotropic hormones; sex hormones;hormones of the average pituitary; hormones of the posterior pituitary. Hormones that regulate the metabolism of calcium and phosphate -Regulation of energy metabolism and its dysfunction in the metabolic syndrome - Control of the metabolism of amino acids and glycides. Transcriptional control of energy metabolism. Molecular basis of the metabolic syndrome. Metabolic changes in tumor cells - General mechanisms for regulating glycolysis in the presence or lack of oxygen, the Pasteur effect, the Warburg effect in tumor cells. Lactic fermentation. The glycolytic pathway and the Krebs cycle for anabolism. Glutamminolysis - Genes and metabolism: MYC and PI3K / AKT PFK1 and PFK2. PFK2 in tumors. Hexokinases in tumor cells. Pyruvate kinases: role of PKM2 in glycolytic metabolism and regulation of gene transcription. Mutations of IDH1, IDH2, FH and SDH genes in tumors: diagnostic role and functional significance. Hypothesis of deregulation of demethylase enzymes in the pathogenesis of neoplasms with mutations in IDH1 and IDH2 genes Alterations of frequent signaling pathways in neoplasms - Alterations of EGFR, MET / HGF, PDGF / PDGFR, MAPK, PI3K / AKT / MTOR pathways in cancer cells and their role in diagnosis and
Expected learning objectivesBeing able to independently assess how the pre-analytical and analytical conditions contribute to define the quality and the informative value of a laboratory result and to choose the most adequate molecular test according to test chemical-physical properties to the characteristics of the marker investigated. To know the main biochemical (protein and metabolic) markers used in laboratory diagnostics to investigate organ function (mainly kidney and liver) and for the diagnosis of inborn metabolic diseases. To know the main signaling pathways downstream of hormones, growth factors and cytokines and the most frequently found alterations in neoplastic diseases useful for diagnostic, prognostic and therapeutic purposes Understanding how various neoplastic and non-neoplastic pathological conditions may be characterized by alterations in energy metabolism and how knowledge of these alterations can be used to identify new therapeutic targets
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CourseClinical Biochemistry and Clinical Molecular Biology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCAPELLO Daniela
TeachersCAPELLO Daniela
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageItalian
Abstract- Proteomic, metabolomic and genomic biomarkers: definition and use. - Preanalytical, analytical and post-analytical factors that influence the laboratory result. Significance and limits of laboratory procedures and results: assessment of the quality and informative value of laboratory results. - Genomic markers and molecular tests: clinical applications of molecular biology. The main molecular biology protocols applied to diagnostics: chemical-physical principles and fields of application - Protein and enzymatic biomarkers used to investigate organ functionality and hereditary metabolic disorders - Main signal transduction pathways in normal cells and their alterations in metabolic disorders and neoplasia. - Representative genetic lesions in cancer employed as diagnostic, predictive and therapeutic markers
Reference textsAntonozzi-Gulletta Medicina di Laboratorio Logica e Patologia Clinica, Piccin
Teaching targetsUnderstanding how metabolic, protein and genetic markers can be used as diagnostic, predictive and therapeutic indicators. Understanding how biochemical and molecular biology techniques are used in diagnostics Understanding the informative value of the enzymatic and metabolic markers in the evaluation of organ functionality and in the diagnosis of metabolic disorders. Acquire the basic knowledge on the use of "genome wide" methodologies used in translational research, applied to the concept of "personalized medicine". Being able to use the theoretical and applicative knowledge of the different biochemical and molecular diagnostic methods as tools to conceive, optimize and apply specific diagnostic procedures in response to various clinical questions To gain an overview on the regulation of metabolic processes in different organs and tissues and to understand how single alterations in the intracellular signaling pathways can compromise the homeostasis not only of the cell or of the tissue, but of the whole organism.
PrerequisitesBasic knowledge on metabolic pathways and energetic metabolism, signal transtuction and regulation of gene expression
Didattics MethodsFrontal lessons with slides presentation. Execution of problems, quizzes and exposition of cases in the classroom to show precise molecular diagnostic problems and guide the student to their resolution. Running problems, quizzes and case resolution at home using the moodle platform. For the preparation of the exam students can use the material provided by the teacher (a pdf copy of the slides projected in class, of handouts prepared by the teacher and of some articles taken from scientific journals on the topics discussed during the course) and on the recommended textbook
Other informationsA pdf copy of the projected slides, the in-depth material and all the information regarding the course and the methods of examination will be made available on the DIR (https://www.dir.uniupo.it/)
Grading rulesThe aim of the exam consists in verifying the level of knowledge skills developed by the student concerning the course program topics. The exam will consist in a written test including 28 questions related to all the topics of the course program divided into: quizzes with multiple choice answers or true / false and 3 exercises that require the resolution of problems concerning the determination of characteristics of a laboratory test (sensitivity, specificity and predictive values). The evaluation is expressed in thirtieths (minimum mark 18) and will consist of a maximum of 28 points for questions with multiple choice answers (1 point for each question) and a maximum of 5 points for the exercises. During the written test it is not allowed to consult any kind of material. The calculator is allowed. The exam allows to evaluate the theoretical knowledge, the critical sense, the ability to understand the text (exercises with a request to make a choice between different alternatives) and the ability to apply the acquired knowledge to practical circumstances (numerical exercises).
Full arguments1. Introduction Biomarkers - Purpose and meaning of diagnostic tests - Proteomic, metabolomic and genomic biomarkers The pre-analytical, analytical and post-analytic steps in laboratory investigations - Variability of biological material and of pre-analytical procedures - Analytical phase: meaning, limits and properties of laboratory procedures. - Post-analytical phase: definition of reference values and threshold values. Diagnostic sensitivity and specificity; predictive values of a diagnostic test 2_ Genomic biomarkers: clinical applications of molecular biology. - Genetic alterations investigated in molecular diagnostics. Potential and limits of molecular tests - Qualitative and quantitative real-time PCR: principles and its application genetic lesions screening - From Sanger sequencing to IV generation sequencing - mRNA sequencing to identify novel molecular markers - Identification of structural DNA variations. Principles and applications in the diagnostics of oncological and congenital diseases 3_Clinical Clinic of organs and systems Protein biomarkers - Plasma proteins: role and quantitative variations - The reactive proteins of the acute phase: classification, regulation of their expression and role in diseases Enzymatic biomarkers - Selection criteria for enzymatic biomarkers in the clinical laboratory. Methods of determination of enzymatic activities - Enzymes used in the clinical laboratory: their physiological function and their use as disease markers. Inborn metabolic alterations - Investigation of the metabolome, of the proteome and the enzymatic dosages: applications and limits. - Definition of diagnostic screening and its applications. - Inborn metabolic alterations investigated by neonatal screening 4_Cell signaling - Functional classification of growth factors, hormones, cytokines and their receptors - Signalling associated with receptors coupled to heterotrimeric proteins G, membrane receptors with or without intrinsic enzymatic activity and intracellular receptors - MAP-kinases, JAK/STAT; PI3K/AKT/mTOR pathways. Small G-proteins family. -Signalling in hormonal regulation: hypothalamic-hypophyseal axis and its peripheral targets; cortical adrenal hormones; gonadotropic hormones; sex hormones;hormones of the average pituitary; hormones of the posterior pituitary. Hormones that regulate the metabolism of calcium and phosphate -Regulation of energy metabolism and its dysfunction in the metabolic syndrome - Control of the metabolism of amino acids and glycides. Transcriptional control of energy metabolism. Molecular basis of the metabolic syndrome. Metabolic changes in tumor cells - General mechanisms for regulating glycolysis in the presence or lack of oxygen, the Pasteur effect, the Warburg effect in tumor cells. Lactic fermentation. The glycolytic pathway and the Krebs cycle for anabolism. Glutamminolysis - Genes and metabolism: MYC and PI3K / AKT PFK1 and PFK2. PFK2 in tumors. Hexokinases in tumor cells. Pyruvate kinases: role of PKM2 in glycolytic metabolism and regulation of gene transcription. Mutations of IDH1, IDH2, FH and SDH genes in tumors: diagnostic role and functional significance. Hypothesis of deregulation of demethylase enzymes in the pathogenesis of neoplasms with mutations in IDH1 and IDH2 genes Alterations of frequent signaling pathways in neoplasms - Alterations of EGFR, MET / HGF, PDGF / PDGFR, MAPK, PI3K / AKT / MTOR pathways in cancer cells and their role in diagnosis and
Expected learning objectivesBeing able to independently assess how the pre-analytical and analytical conditions contribute to define the quality and the informative value of a laboratory result and to choose the most adequate molecular test according to test chemical-physical properties to the characteristics of the marker investigated. To know the main biochemical (protein and metabolic) markers used in laboratory diagnostics to investigate organ function (mainly kidney and liver) and for the diagnosis of inborn metabolic diseases. To know the main signaling pathways downstream of hormones, growth factors and cytokines and the most frequently found alterations in neoplastic diseases useful for diagnostic, prognostic and therapeutic purposes Understanding how various neoplastic and non-neoplastic pathological conditions may be characterized by alterations in energy metabolism and how knowledge of these alterations can be used to identify new therapeutic targets
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CourseDissertation Thesis
Course IDBT063
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
CFU10
Individual study time 250
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryE - Prova finale e lingua straniera
Year3
Frequenza obbligatoriaYes
Grading typeFinal grade
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CourseElective Didactic Activities
Course IDMC117
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
CFU12
Individual study time 300
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryD - A scelta dallo studente
Year3
Frequenza obbligatoriaYes
Grading typeFinal judgment
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CourseEpidemiology
Course IDMS0448
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBARONE ADESI Francesco
TeachersFAGGIANO Fabrizio, BARONE ADESI Francesco, ALLARA Elias
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/42 - IGIENE GENERALE E APPLICATA
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseEpidemiology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderBARONE ADESI Francesco
TeachersFAGGIANO Fabrizio, BARONE ADESI Francesco
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageItalian
AbstractFundamentals of Philosophy of Science applied to biomedical research;Epidemiological methods in biomedical research; the Syntesis of knowledge in biomedical research and its transfer into medical practice; the process of drug discovery and development; the communication of the results in biomedical research; Ethical Issues in biomedical research
Reference textsFaggiano, Donato, Barbone. Manuale di Epidemiologia per la sanità pubblica. Centro Scientifico Editore. Rothman. Epidemiology: An Introduction. Oxford press.
Teaching targetsKNOWLEDGE AND UNDERSTANDING To know the most basic concepts of philosophy of science applied to biomedical research To know the most important sources of epidemiological data. To understand the elements in the design and conduct of the most important types of epidemiological studies. To know the most important sources of bias in epidemiological studies. to understand the criteria for characterizing the causality of associations in Medicine. to know the key features of a systematic review of the scientific literature to know the key features of the process of drug discovery and development. to understand the key features of Evidence-Based Medicine. To know the basic features of the communication of results in biomedicalresearch To know the most important ethical aspects of biomedical research. APPLYING KNOWLEDGE AND UNDERSTANDING to be able to calculate measures of frequency and association to be able to interpret the results of an epidemiological study to be able to interpret the results of a meta-analysis
PrerequisitesNone
Didattics MethodsLectures
Other informationsNone
Grading rulesWritten examination, during which it will be evaluated the ability of the student of applying the knowledge acquired during the course. The written exam is constituted of 13-15 questions of the following types: multiple choice questions; calculation of epidemiological measures introduced in the course. The level of difficulty of the exercises corresponds to those explained in class or presented in the handbooks reported in DIR. The pass level is 18/30. During the exam the student is not allowed to use any type of teaching material.
Full argumentsFundamentals of Philosophy of Science applied to biomedical research. The scientific method. The concepts of model, theory and hypothesis in science. Inductive and deductive reasoning. Epidemiological methods in biomedical research. The concept of Health and its evolution over time. Definition of Hygiene and Public Health. Introduction to medical statistics (sources of data; fonti; elaboration and presentation of epidemiological data; health indicators: incidence, mortality, prevalence, survival; measures of frequency and association). Main Epidemiological studies: clinical trials, cohort studies, case-control studies, cross-sectional studies, descriptive studies. Sources of uncertainty in epidemiology: the role of chance, bias and counfounding. Effect modification. Use of test of hypothesis and confidence intervals to interpret the results of an epidemiological study. The concept of statistical power applied to the interpretation of the results of an epidemiological study. Causal inferenc ein epidemiology. the Syntesis of knowledge in biomedical research and its transfer into medical practice. Systematic reviews, meta-analyses, guide-lines, Evidence-Based Medicine. The process of drug discovery and development. Epidemiological studies used in the different stages of the development of a drug. Introduction to pharmacoepidemiology and its applications to to the study of Adverse Drug Events. the communication of the results in biomedical research. Structure of a scientific report. The process of submission and reviewing of a scientific report. The scientific press. Ethical Issues in biomedical research. The conflict of interest. Disease mongering.
Expected learning objectivesKNOWLEDGE AND UNDERSTANDING To know the most important sources of epidemiological data. To understand the elements in the design and conduct of the most important types of epidemiological studies. To know the most important sources of bias in epidemiological studies. to understand the criteria for characterizing the causality of associations in Medicine. to know the key features of a systematic review of the scientific literature to know the most important determinants of health in Italy and worldwide to know the differences between primary, secondary and tertiary prevention. to know the key features of the process of drug discovery and development. to understand the key features of Evidence-Based Medicine. to know the key features of National Health Systems. APPLYING KNOWLEDGE AND UNDERSTANDING to be able to calculate measures of frequency and association to be able to calculate sensitivity, specificity, and predictive values of a clinical test to able to interpret the results of a meta-analysis
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CourseEpidemiology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderALLARA Elias
TeachersALLARA Elias
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageItalian
AbstractFundamentals of Philosophy of Science applied to biomedical research;Epidemiological methods in biomedical research; the Syntesis of knowledge in biomedical research and its transfer into medical practice; the process of drug discovery and development; the communication of the results in biomedical research; Ethical Issues in biomedical research
Reference textsFaggiano, Donato, Barbone. Manuale di Epidemiologia per la sanità pubblica. Centro Scientifico Editore. Rothman. Epidemiology: An Introduction. Oxford press.
Teaching targetsKNOWLEDGE AND UNDERSTANDING To know the most basic concepts of philosophy of science applied to biomedical research To know the most important sources of epidemiological data. To understand the elements in the design and conduct of the most important types of epidemiological studies. To know the most important sources of bias in epidemiological studies. to understand the criteria for characterizing the causality of associations in Medicine. to know the key features of a systematic review of the scientific literature to know the key features of the process of drug discovery and development. to understand the key features of Evidence-Based Medicine. To know the basic features of the communication of results in biomedicalresearch To know the most important ethical aspects of biomedical research. APPLYING KNOWLEDGE AND UNDERSTANDING to be able to calculate measures of frequency and association to be able to interpret the results of an epidemiological study to be able to interpret the results of a meta-analysis
PrerequisitesNone
Didattics MethodsLectures
Other informationsNone
Grading rulesWritten examination, during which it will be evaluated the ability of the student of applying the knowledge acquired during the course. The written exam is constituted of 13-15 questions of the following types: multiple choice questions; calculation of epidemiological measures introduced in the course. The level of difficulty of the exercises corresponds to those explained in class or presented in the handbooks reported in DIR. The pass level is 18/30. During the exam the student is not allowed to use any type of teaching material.
Full argumentsFundamentals of Philosophy of Science applied to biomedical research. The scientific method. The concepts of model, theory and hypothesis in science. Inductive and deductive reasoning. Epidemiological methods in biomedical research. The concept of Health and its evolution over time. Definition of Hygiene and Public Health. Introduction to medical statistics (sources of data; fonti; elaboration and presentation of epidemiological data; health indicators: incidence, mortality, prevalence, survival; measures of frequency and association). Main Epidemiological studies: clinical trials, cohort studies, case-control studies, cross-sectional studies, descriptive studies. Sources of uncertainty in epidemiology: the role of chance, bias and counfounding. Effect modification. Use of test of hypothesis and confidence intervals to interpret the results of an epidemiological study. The concept of statistical power applied to the interpretation of the results of an epidemiological study. Causal inferenc ein epidemiology. the Syntesis of knowledge in biomedical research and its transfer into medical practice. Systematic reviews, meta-analyses, guide-lines, Evidence-Based Medicine. The process of drug discovery and development. Epidemiological studies used in the different stages of the development of a drug. Introduction to pharmacoepidemiology and its applications to to the study of Adverse Drug Events. the communication of the results in biomedical research. Structure of a scientific report. The process of submission and reviewing of a scientific report. The scientific press. Ethical Issues in biomedical research. The conflict of interest. Disease mongering.
Expected learning objectivesKNOWLEDGE AND UNDERSTANDING To know the most important sources of epidemiological data. To understand the elements in the design and conduct of the most important types of epidemiological studies. To know the most important sources of bias in epidemiological studies. to understand the criteria for characterizing the causality of associations in Medicine. to know the key features of a systematic review of the scientific literature to know the most important determinants of health in Italy and worldwide to know the differences between primary, secondary and tertiary prevention. to know the key features of the process of drug discovery and development. to understand the key features of Evidence-Based Medicine. to know the key features of National Health Systems. APPLYING KNOWLEDGE AND UNDERSTANDING to be able to calculate measures of frequency and association to be able to calculate sensitivity, specificity, and predictive values of a clinical test to able to interpret the results of a meta-analysis
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CourseFurther Learning Activities
Course IDMS0120
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
CFU2
Individual study time 100
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryF - Stage e altre attività formative
Year3
Frequenza obbligatoriaYes
Grading typeFinal judgment
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CourseGeneral Pathology
Course IDMS0447
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCARINI Rita
TeachersCARINI Rita
CFU6
Teaching duration (hours)48
Individual study time 102
SSDMED/04 - PATOLOGIA GENERALE
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseGeneral Pathology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCARINI Rita
TeachersCARINI Rita
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageItalian
Abstract1) INTRODUCTION TO GENERAL PATHOLOGY 2) THE GENETIC BASE OF THE DISEASES 3) PHYSICAL AGENTS AS CAUSE OF DISEASES 4) CHEMICAL AGENTS AS CAUSE OF DISEASES 5) BIOLOGICAL AGENTS AS CAUSE OF DISEASES 6) TISSUE MODIFICATIONS IN RESPONSE TO PATHOLOGICAL CHRONIC AND ACUTE STIMULA 7) INFLAMMATORY PROCESS 8) REPAIR PROCESS 9) NEOPLASTIC GROWTH
Reference textsPatologia e Fisiopatologia Generale di Pontieri, Russo, Frati (Ed. Piccin) Istituzioni di Patologia Generale. M.U. Dianzani (ed. UTET) La Professione del Medico vol.3, (ed UTET)
Teaching targetsThe specific aim of the PATOLOGIA GENERALE course is to understand the root causes (etiology) and mechanisms (pathogenesis) that are responsible for altering the state of health. To this end, the molecular, cellular and super-cellular effects of endogenous pathogens (genetic and tumor diseases) and exogenous pathogens (physical, chemical and biological causes of disease) will be examined. Cell and tissue levels will then be investigated for the adverse consequences of chronic and acute pacing with pathogens (degeneration, reversible and irreversible cell damage, cell death for necrosis or apoptosis). The local and systemic reaction to tissue damage will be described by thorough examination of the inflammatory process and consequent tissue repair. The course of GENERAL PATHOLOGY conducted for the Student of first Degree in BIOTECHNOLOGIES within the Extended Program reported below, will examine in particular the molecular mechanisms of the various pathological conditions examined with specific references to the implications for future Medical Research activities.
PrerequisitesThe Course needs the previous knowledge of fundamental elements of biochemistry, biology, genetics and physiology acquired in previous or parallel courses, which will provide the basis for the learning of the effects of exogenous and endogenous disease agents on the cellular and tissue levels ( Cell damage and neoplastic transformation) and non-specific (inflammatory) and specific (immune) defense responses.
Didattics MethodsLectures based on a continuous student interaction that will allow immediate verification of understanding of the topics discussed. Strongly recommended is the acquisition of notes of all the lessons directly taken by the student such notes will be essential for the preparation of the exam. All slides of the course will be available since from the first lesson.
Grading rulesWe will evaluate the students with a written examination, with questions and multiple choice answers. The questions will be distributed to cover the all program. The questions will be prepared to both verify the knowledge and the understanding of single aspects of the program.
Full argumentsINTRODUCTION TO GENERAL PATHOLOGY; The state of health, the concept of etiology, the concept of pathogenesis, the state of illness. GENETIC DISEASE BASES General on genetic mutations and their causes. Examples of genetic diseases: sickle cell anemia and alterations in transport or metabolism of amino acids: Hartnup disease, phenylalaninemia, albinism. PHYSICAL AGENTS AS A CAUSE OF DISEASE: generality about mechanical, thermal, electric power transfer pathologies. Effects of ionizing radiation (molecular, cellular and super-cellular) and the effects of exciting radiations. CHEMICAL AGENTS AS A DISEASE: absorption and elimination routes; biotransformation reactions: phase 1 (dependent on cytochrome P450) and phase 2; adverse effects of reactive metabolites produced during biotransformation), toxicity by chemical agents . The damage caused by free radicals: production mechanisms, protective factors and cell damage. BIOLOGICAL AGENTS AS A CAUSE OF DISEASE: Generalities on the mechanisms of damage and defense against viral and bacterial infections. TISSUE CHANGES IN RESPONSE TO CHRONIC AND ACUTE PATHOLOGICAL STIMULES: hypertrophy, hyperplasia, hypotrophy, atrophy, metaplasia; Reversible cell damage. Irreversible cell damage: cell death for necrosis and apoptosis. INFLAMMATORY PROCESS: Inflammatory cells and their functions (monocytes, macrophages, neutrophil granulocytes, basophils, mast cells, endothelial cells, platelets) soluble inflammatory mediators (histamine, serotonin, arachidonic acid metabolites, plasma proteases, cytokines: interleukin 1.6 [IL-1 and IL-6], tumor necrosis factor alpha [TNFalfa], transformant-beta growth factor [TGFbeta], bacterial metabolites). Alteration of the tone and vascular permeability during inflammation (exudate formation). Activity of phagocytic cells in inflammation (adhesion, diaphytesis, chemotaxis, phagocytosis, bacterial killing mechanisms); istoflogosi. Systemic effects of inflammation. REPAIR PROCESS: regeneration (tissue proliferative potential, growth factors: epidermal growth factor [EGF], transformant [TGFbeta], similar insulin [IGF], platelet derivation [PDGF], fibroblasts [FGF], interactions With matrix and other cells) substitution with connective tissue and angiogenesis. Wound repair NEOPLASTIC GROWTH: General characteristics of the neoplasms (information on incidence, mortality and survival, tumor classification and nomenclature); Hyperplasia, neoplasia, anaplasia and dysplasia. Viral and cellular oncogenes, oncosoppressor genes. Neoplastic phenotype: phenotypic heterogeneity and genomic instability. Initiation, promotion of the neoplastic process (carcinogenic stages or cancerogenesis as a continuous process). The progression of the neoplastic process (neoplastic latency, neoplastic invasion, metastases).
Expected learning objectivesThe student is expected to obtain the knowledge and the understanding of the molecular base of the loss of health (disease status). Specifically the student is expected to know the causes and the pathogenetic mechanisms of fundamental diseases induced by physical, chemical, biological agents and by genetic alterations; the mechanisms of cell damage, inflammation, reparation and tumor transformation.
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CourseGeneral Pathology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCARINI Rita
TeachersCARINI Rita
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageItalian
Abstract1) INTRODUCTION TO GENERAL PATHOLOGY 2) THE GENETIC BASE OF THE DISEASES 3) PHYSICAL AGENTS AS CAUSE OF DISEASES 4) CHEMICAL AGENTS AS CAUSE OF DISEASES 5) BIOLOGICAL AGENTS AS CAUSE OF DISEASES 6) TISSUE MODIFICATIONS IN RESPONSE TO PATHOLOGICAL CHRONIC AND ACUTE STIMULA 7) INFLAMMATORY PROCESS 8) REPAIR PROCESS 9) NEOPLASTIC GROWTH
Reference textsPatologia e Fisiopatologia Generale di Pontieri, Russo, Frati (Ed. Piccin) Istituzioni di Patologia Generale. M.U. Dianzani (ed. UTET) La Professione del Medico vol.3, (ed UTET)
Teaching targetsThe specific aim of the PATOLOGIA GENERALE course is to understand the root causes (etiology) and mechanisms (pathogenesis) that are responsible for altering the state of health. To this end, the molecular, cellular and super-cellular effects of endogenous pathogens (genetic and tumor diseases) and exogenous pathogens (physical, chemical and biological causes of disease) will be examined. Cell and tissue levels will then be investigated for the adverse consequences of chronic and acute pacing with pathogens (degeneration, reversible and irreversible cell damage, cell death for necrosis or apoptosis). The local and systemic reaction to tissue damage will be described by thorough examination of the inflammatory process and consequent tissue repair. The course of GENERAL PATHOLOGY conducted for the Student of first Degree in BIOTECHNOLOGIES within the Extended Program reported below, will examine in particular the molecular mechanisms of the various pathological conditions examined with specific references to the implications for future Medical Research activities.
PrerequisitesThe Course needs the previous knowledge of fundamental elements of biochemistry, biology, genetics and physiology acquired in previous or parallel courses, which will provide the basis for the learning of the effects of exogenous and endogenous disease agents on the cellular and tissue levels ( Cell damage and neoplastic transformation) and non-specific (inflammatory) and specific (immune) defense responses.
Didattics MethodsLectures based on a continuous student interaction that will allow immediate verification of understanding of the topics discussed. Strongly recommended is the acquisition of notes of all the lessons directly taken by the student such notes will be essential for the preparation of the exam. All slides of the course will be available since from the first lesson.
Grading rulesWe will evaluate the students with a written examination, with questions and multiple choice answers. The questions will be distributed to cover the all program. The questions will be prepared to both verify the knowledge and the understanding of single aspects of the program.
Full argumentsINTRODUCTION TO GENERAL PATHOLOGY; The state of health, the concept of etiology, the concept of pathogenesis, the state of illness. GENETIC DISEASE BASES General on genetic mutations and their causes. Examples of genetic diseases: sickle cell anemia and alterations in transport or metabolism of amino acids: Hartnup disease, phenylalaninemia, albinism. PHYSICAL AGENTS AS A CAUSE OF DISEASE: generality about mechanical, thermal, electric power transfer pathologies. Effects of ionizing radiation (molecular, cellular and super-cellular) and the effects of exciting radiations. CHEMICAL AGENTS AS A DISEASE: absorption and elimination routes; biotransformation reactions: phase 1 (dependent on cytochrome P450) and phase 2; adverse effects of reactive metabolites produced during biotransformation), toxicity by chemical agents . The damage caused by free radicals: production mechanisms, protective factors and cell damage. BIOLOGICAL AGENTS AS A CAUSE OF DISEASE: Generalities on the mechanisms of damage and defense against viral and bacterial infections. TISSUE CHANGES IN RESPONSE TO CHRONIC AND ACUTE PATHOLOGICAL STIMULES: hypertrophy, hyperplasia, hypotrophy, atrophy, metaplasia; Reversible cell damage. Irreversible cell damage: cell death for necrosis and apoptosis. INFLAMMATORY PROCESS: Inflammatory cells and their functions (monocytes, macrophages, neutrophil granulocytes, basophils, mast cells, endothelial cells, platelets) soluble inflammatory mediators (histamine, serotonin, arachidonic acid metabolites, plasma proteases, cytokines: interleukin 1.6 [IL-1 and IL-6], tumor necrosis factor alpha [TNFalfa], transformant-beta growth factor [TGFbeta], bacterial metabolites). Alteration of the tone and vascular permeability during inflammation (exudate formation). Activity of phagocytic cells in inflammation (adhesion, diaphytesis, chemotaxis, phagocytosis, bacterial killing mechanisms); istoflogosi. Systemic effects of inflammation. REPAIR PROCESS: regeneration (tissue proliferative potential, growth factors: epidermal growth factor [EGF], transformant [TGFbeta], similar insulin [IGF], platelet derivation [PDGF], fibroblasts [FGF], interactions With matrix and other cells) substitution with connective tissue and angiogenesis. Wound repair NEOPLASTIC GROWTH: General characteristics of the neoplasms (information on incidence, mortality and survival, tumor classification and nomenclature); Hyperplasia, neoplasia, anaplasia and dysplasia. Viral and cellular oncogenes, oncosoppressor genes. Neoplastic phenotype: phenotypic heterogeneity and genomic instability. Initiation, promotion of the neoplastic process (carcinogenic stages or cancerogenesis as a continuous process). The progression of the neoplastic process (neoplastic latency, neoplastic invasion, metastases).
Expected learning objectivesThe student is expected to obtain the knowledge and the understanding of the molecular base of the loss of health (disease status). Specifically the student is expected to know the causes and the pathogenetic mechanisms of fundamental diseases induced by physical, chemical, biological agents and by genetic alterations; the mechanisms of cell damage, inflammation, reparation and tumor transformation.
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CoursePharmaceutical Chemistry
Course IDMS0449
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPIRALI Tracey
TeachersSORBA Giovanni, PIRALI Tracey, MASSAROTTI Alberto
CFU5
Teaching duration (hours)40
Individual study time 85
SSDCHIM/08 - CHIMICA FARMACEUTICA
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodSecondo Semestre
Frequenza obbligatoriaYes
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CoursePharmaceutical Chemistry
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPIRALI Tracey
TeachersSORBA Giovanni, PIRALI Tracey, MASSAROTTI Alberto
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageitalian
AbstractMedicinal Chemistry definition. Pharmaceutical Phase Routes of administration. Pharmacokinetic Phase Absorption. Distribution. Elimination. Metabolism. Excretion. Pharmacodynamic Phase Intermolecular interactions. Role of steoreochemistry in the interaction with the binding site. Receptors as drug targets. Enzymes as drug targets. Miscellaneous drug targets. Drug discovey Hit compound and lead compound. Finding a lead. How to recognize drug-like molecules. Molecular modelling. Drug design The optimization of target interactions. The optimization of the access to the target. Drug development Preclinical and clinical trials. Examples of classes of drugs. Biotech drugs. Exercises.
Reference textsChimica Farmaceutica Graham L. Patrick Foye’s Principi di Chimica Farmaceutica Thomas L. Lemke, David A. Williams Medicinal Chemistry: The Modern Drug Discovery Process, E. Stevens Chimica Farmaceutica A. Gasco
Teaching targetsThe aim of the course is to introduce the student to the main principles of medicinal chemistry. The specific objectives of the course, according to the Dublino descriptors, are the following: 1. Knowledge and understanding. At the end of the course the student will know and understand the basic concepts of medicinal chemistry, in particular the route followed by the drug in the organism, from its administration to its excretion (pharmaceutical, pharmacokinetic, pharmacodynamic phases). Moreover, the itinerary of the drug in the pharmaceutical industry, from drug discovery to drug development through drug design, will be addressed. 2. The student will apply the acquired knowledge and understanding in the recognition of the chemical structures, in the discussion of the chemical and chemico-physical properties, of the mechanism of action, of the structure-activity relationship, of the metabolic fate and of the plausible routes for the synthesis of drugs. 3. The student will acquire the ability to identify and use data to formulate responses to well-defined concrete and abstract problems regarding the structure-activity relationship, the metabolism, the related toxicity, the synthesis, the drug-drug interaction for specific cases of drugs. Moreover, the student will be provided with all the tools necessary for the critical analysis of texts and papers in the medicinal chemistry literature. 4. The student will learn to communicate about his/her understanding, to describe, even in an original way, a topic described during lessons, to adequately answer to questions, critiques and suggestions. 5. The student will have the learning skills to handle in a dinamic way his/her knowledge in medicinal chemistry with some autonomy.
PrerequisitesIn order to give the exam in Medicinal Chemistry I it is necessary that the student has passed the exam in Organic Chemistry
Didattics MethodsThe course is given with the support of power point slides, which are given to the students at the beginning of the lessons. Moreover, the student will be introduced to the use of freeware softwares of molecular visualisation in order to handle and understand the chemical structures proposed during the lessons. Finally, practice exercises, with the aim of preparing the student to the exam, will be solved during the lessons.
Grading rulesThe exam consists in a computer-based written test displaying questions (multiple choice). The exam aims at verifying both the knowledge on the contents of the course and the ability to apply this knowledge to specific cases of drugs used in therapy. The student has to demonstrate to be able to o identify the functional groups, the acid, basic and stereogenic centers, the plausible reactions of metabolism, the intermolecular interactions, etc. Through these tests, it will be verified that the student has achieved the objectives of knowledge and understanding of the contents of the course and the competence to apply the acquired knowledge and skills to specific concerns regarding the medicinal chemistry of the different classes of drugs.
Full argumentsMedicinal Chemistry definition. Pharmaceutical Phase Routes of administration. Pharmacokinetic Phase Absorption. Mechanisms of absorption. Passive diffusion. Partition coefficient. Fick law. Henderson-Hasselbach equation. Ion-pair absorption. Carrier mediated transport. Vescicolar transport. Convective trasport. The absorption in the oral administration: first-pass metabolism. Distribution. Plasma protein binding. Accumulation. Blood-brain barrier. Placental barrier. Elimination. Renal excretion. Phase I and phase II metabolism. Soft e hard drugs. Pharmacokinetic concepts. Plasma concentration-time curve. Clearance. Half-life. Bioavailability. Apparent volume of distribution. Factors that influence pharmacokinetics. Pharmacodynamic Phase Intermolecular interactions. Covalent bond. Ionic bond. Dipole-dipole interaction. Ion-dipole interaction. Hydrogen bond. Halogen bond. Charge transfer. Van der Waals interactions. Hydrophobic interactions. Pi greco-pi greco interactions. Role of steoreochemistry in the interaction with the binding site. Three point contact model. Pfeiffer rule. Eutomer, distomer, eudismic ratio. Chiral switch. Methods for the chiral switch. Receptors as drug targets: theories, strategies to design agonists, antagonists, allosteric modulators, inverse agonists. Enzymes as drug targets: competitive reversible, non competitive reversible, irreversible inhibitors, transition-state analogues, suicide substrates. Miscellaneous drug targets: transport proteins as drug targets, structural proteins as drug targets, protein-protein interactions, lipids as drug targets. Hit compound and lead compound. Choosing a disease, a drug target, a bioassay. High-throughput screening, NMR screening, virtual screening. Parallel and combinatorial synthesis. Finding a lead: natural products, serendipity, natural ligand modifications, me-too drugs, SOSA approach, screening, de novo drug design, fragment-based drug design. How to recognize drug-like molecules. Lipinski and Veber rules. The use of computer in drug discovery. Manipolation of chemical structures, conformational analysis, minimization, parametrization. The use of chemical and biological data banks, chemical similarity and X-ray structures. Ligand- and Structure-Based Drug Design. Virtual screening. De novo drug design. 3D-QSAR. Pharmacophore- Molecular docking, applications and drawbacks. Proteins. Resolution in 3D protein structures. Examples of protein purification. X ray cristallography. NMR spectroscopy. Co-cristallization. Quality of experimental data: resolution and B-factor. Protein Data Bank. On-line search of PDB. The structure of a .pdb. PyMol, main functions of the program. Basic procedure to visualize a .pdb in the PyMol software. Drug design The optimization of target interactions: structure-activity relationships, isosteres and bioisosteres, classic and non classic bioisosteres, pharmacophore identification, drug design strategies (extension, chain extension/contraction, homology, vinilogy, benzology, ring expansion/contraction, ring variations, ring fusion, structure simplification, structure rigidification, conformational blockers, twin drugs and hybrids). The optimization of the access to the target: how to optimize the hydrophobic and hydrophilic properties, how to improve the chemical and the metabolic stability, prodrugs, mutue prodrugs. Drug development Preclinical and clinical trials. Examples of specific classes of drugs. Biotech drugs. Exercises.
Expected learning objectivesThe student at the end of the course will know the basic principles of medicinal chemistry, both in pharmacodinamics and pharmacokinetics. Furthermore, he/she will be able to apply these principles to a specific bioactive molecule and to evaluate its chemical, metabolic and drug-likeness properties. The student will also know the role of medicinal chemistry in drug discovery and in drug design and the strategies that can be used in order to improve the pharmacodinamic and pharmacokinetic properties of a bioactive molecule.
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CoursePharmaceutical Chemistry
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderPIRALI Tracey
TeachersSORBA Giovanni, PIRALI Tracey, MASSAROTTI Alberto
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageitalian
AbstractMedicinal Chemistry definition. Pharmaceutical Phase Routes of administration. Pharmacokinetic Phase Absorption. Distribution. Elimination. Metabolism. Excretion. Pharmacodynamic Phase Intermolecular interactions. Role of steoreochemistry in the interaction with the binding site. Receptors as drug targets. Enzymes as drug targets. Miscellaneous drug targets. Drug discovey Hit compound and lead compound. Finding a lead. How to recognize drug-like molecules. Molecular modelling. Drug design The optimization of target interactions. The optimization of the access to the target. Drug development Preclinical and clinical trials. Examples of classes of drugs. Biotech drugs. Exercises.
Reference textsChimica Farmaceutica Graham L. Patrick Foye’s Principi di Chimica Farmaceutica Thomas L. Lemke, David A. Williams Medicinal Chemistry: The Modern Drug Discovery Process, E. Stevens Chimica Farmaceutica A. Gasco
Teaching targetsThe aim of the course is to introduce the student to the main principles of medicinal chemistry. The specific objectives of the course, according to the Dublino descriptors, are the following: 1. Knowledge and understanding. At the end of the course the student will know and understand the basic concepts of medicinal chemistry, in particular the route followed by the drug in the organism, from its administration to its excretion (pharmaceutical, pharmacokinetic, pharmacodynamic phases). Moreover, the itinerary of the drug in the pharmaceutical industry, from drug discovery to drug development through drug design, will be addressed. 2. The student will apply the acquired knowledge and understanding in the recognition of the chemical structures, in the discussion of the chemical and chemico-physical properties, of the mechanism of action, of the structure-activity relationship, of the metabolic fate and of the plausible routes for the synthesis of drugs. 3. The student will acquire the ability to identify and use data to formulate responses to well-defined concrete and abstract problems regarding the structure-activity relationship, the metabolism, the related toxicity, the synthesis, the drug-drug interaction for specific cases of drugs. Moreover, the student will be provided with all the tools necessary for the critical analysis of texts and papers in the medicinal chemistry literature. 4. The student will learn to communicate about his/her understanding, to describe, even in an original way, a topic described during lessons, to adequately answer to questions, critiques and suggestions. 5. The student will have the learning skills to handle in a dinamic way his/her knowledge in medicinal chemistry with some autonomy.
PrerequisitesIn order to give the exam in Medicinal Chemistry I it is necessary that the student has passed the exam in Organic Chemistry
Didattics MethodsThe course is given with the support of power point slides, which are given to the students at the beginning of the lessons. Moreover, the student will be introduced to the use of freeware softwares of molecular visualisation in order to handle and understand the chemical structures proposed during the lessons. Finally, practice exercises, with the aim of preparing the student to the exam, will be solved during the lessons.
Grading rulesThe exam consists in a computer-based written test displaying questions (multiple choice). The exam aims at verifying both the knowledge on the contents of the course and the ability to apply this knowledge to specific cases of drugs used in therapy. The student has to demonstrate to be able to o identify the functional groups, the acid, basic and stereogenic centers, the plausible reactions of metabolism, the intermolecular interactions, etc. Through these tests, it will be verified that the student has achieved the objectives of knowledge and understanding of the contents of the course and the competence to apply the acquired knowledge and skills to specific concerns regarding the medicinal chemistry of the different classes of drugs.
Full argumentsMedicinal Chemistry definition. Pharmaceutical Phase Routes of administration. Pharmacokinetic Phase Absorption. Mechanisms of absorption. Passive diffusion. Partition coefficient. Fick law. Henderson-Hasselbach equation. Ion-pair absorption. Carrier mediated transport. Vescicolar transport. Convective trasport. The absorption in the oral administration: first-pass metabolism. Distribution. Plasma protein binding. Accumulation. Blood-brain barrier. Placental barrier. Elimination. Renal excretion. Phase I and phase II metabolism. Soft e hard drugs. Pharmacokinetic concepts. Plasma concentration-time curve. Clearance. Half-life. Bioavailability. Apparent volume of distribution. Factors that influence pharmacokinetics. Pharmacodynamic Phase Intermolecular interactions. Covalent bond. Ionic bond. Dipole-dipole interaction. Ion-dipole interaction. Hydrogen bond. Halogen bond. Charge transfer. Van der Waals interactions. Hydrophobic interactions. Pi greco-pi greco interactions. Role of steoreochemistry in the interaction with the binding site. Three point contact model. Pfeiffer rule. Eutomer, distomer, eudismic ratio. Chiral switch. Methods for the chiral switch. Receptors as drug targets: theories, strategies to design agonists, antagonists, allosteric modulators, inverse agonists. Enzymes as drug targets: competitive reversible, non competitive reversible, irreversible inhibitors, transition-state analogues, suicide substrates. Miscellaneous drug targets: transport proteins as drug targets, structural proteins as drug targets, protein-protein interactions, lipids as drug targets. Hit compound and lead compound. Choosing a disease, a drug target, a bioassay. High-throughput screening, NMR screening, virtual screening. Parallel and combinatorial synthesis. Finding a lead: natural products, serendipity, natural ligand modifications, me-too drugs, SOSA approach, screening, de novo drug design, fragment-based drug design. How to recognize drug-like molecules. Lipinski and Veber rules. The use of computer in drug discovery. Manipolation of chemical structures, conformational analysis, minimization, parametrization. The use of chemical and biological data banks, chemical similarity and X-ray structures. Ligand- and Structure-Based Drug Design. Virtual screening. De novo drug design. 3D-QSAR. Pharmacophore- Molecular docking, applications and drawbacks. Proteins. Resolution in 3D protein structures. Examples of protein purification. X ray cristallography. NMR spectroscopy. Co-cristallization. Quality of experimental data: resolution and B-factor. Protein Data Bank. On-line search of PDB. The structure of a .pdb. PyMol, main functions of the program. Basic procedure to visualize a .pdb in the PyMol software. Drug design The optimization of target interactions: structure-activity relationships, isosteres and bioisosteres, classic and non classic bioisosteres, pharmacophore identification, drug design strategies (extension, chain extension/contraction, homology, vinilogy, benzology, ring expansion/contraction, ring variations, ring fusion, structure simplification, structure rigidification, conformational blockers, twin drugs and hybrids). The optimization of the access to the target: how to optimize the hydrophobic and hydrophilic properties, how to improve the chemical and the metabolic stability, prodrugs, mutue prodrugs. Drug development Preclinical and clinical trials. Examples of specific classes of drugs. Biotech drugs. Exercises.
Expected learning objectivesThe student at the end of the course will know the basic principles of medicinal chemistry, both in pharmacodinamics and pharmacokinetics. Furthermore, he/she will be able to apply these principles to a specific bioactive molecule and to evaluate its chemical, metabolic and drug-likeness properties. The student will also know the role of medicinal chemistry in drug discovery and in drug design and the strategies that can be used in order to improve the pharmacodinamic and pharmacokinetic properties of a bioactive molecule.
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CoursePharmacology and Innovation management
Course IDMS0432
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderFRESU Luigia Grazia
TeachersCONICELLA Fabrizio, FALVO Sara, FRESU Luigia Grazia, TRAVELLI Cristina, SEDDIO Pasquale, JOMMI Claudio
CFU12
Course typeAttività formativa integrata
Course mandatorietyMandatory course
Year3
PeriodPrimo Semestre
Grading typeFinal grade
Teaching languageItalian
AbstractPHARMACOLOGY: Preclinical and clinical development of drugs. Principles of pharmacokinetics and pharmacodynamics. Main classes of drugs. Originator drug concept, generic drug, biosimilar medication. Main classes of drugs. MANAGEMENT: Creativity, scientific result and innovation. From idea to innovation: the management of innovative projects and project management. Sources of patent information. Management and enhancement of the results of the innovative process: patents, new companies and innovation within the organization. The promotion of innovation: the marketing of innovations. ECONOMY:From the scientific result to the company: the forms of technological transfer. Introduction to management. Management of biotech inventions. Biotech market and industry. Economic evaluation of (red) biotechnologies, with a focus on biotech medicines.
Reference textsMANAGEMENTSlide of the lectures + suggested pdf articles Books: Managing Innovation: Integrating Technological, Market and Organizational Change, Joe Tidd, John Bessant March 2009 Innovation and Entrepreneurship John Bessant Joe Tidd June 2011 John Wiley & Sons Inc; La Gestione Del Trasferimento Tecnologico - Strategie, Modelli E Strumenti Andrea Piccaluga, Massimiliano Granieri, Giuseppe Conti 1 edizione (25 agosto 2011) Ricerca e sviluppo nell'industria biotecnologica e farmaceutica Silvano Fumero Bollati Boringhieri (23 maggio 2003) Project Management: dall'idea all'attuazione. Una guida pratica per il successo Marion E. Haynes Editore: Franco Angeli (11 ottobre 2012) Commercializing Successful Biomedical Technologies: Basic Principles for the Development of Drugs, Diagnostics and Devices Shreefal S. Mehta Cambridge University Press; Building Biotechnology: Biotechnology Business, Regulations, Patents, Law, Policy and Science Yali Friedman Editore: Logos Press; 4. Auflage. PHARMACOLOGY 1. HP Rang and MM Dale – Farmacologia, Casa Ed Ambrosiana 2. Goodman & Gilman – Le basi farmacologiche della terapia di J.G.Hardman, L.E. Limbird, A.Goodman Gilman, Curatore edizione italiana: C.Sirtori, G.Folco, G.Franceschini, S.Govoni. 3. Farmacologia generale e molecolare, a cura di F.Clementi, G.Fumagalli, Ed UTET John Wiley & Sons Inc ECONOMY There is not a single textbook. For Management readings will be mentioned during the course. Management of biotech inventions Friedman Y (2013), Building Biotechnology: Biotechnology Business, Regulations, Patents, Law, ThinkBiotech LLC, 4th Edition Bio-pharma industry Gianfrate F, Il mercato dei farmaci tra salute e business, Franco Angeli Editore, 2014 Economic evaluation of red biotechnologies Mennini FS, Cicchetti A, Fattore G, Russo P. La Valutazione Economica dei Programmi Sanitari. Il Pensiero Scientifico ed. 2011. Terza Edizione.
Teaching targetsPHARMACOLOGY. In the context of this Integrated Course, the module of Pharmacology will give to students the basis of pharmacokinetic and pharmacodynamic principles, the general concepts of “conventional small molecules” and biological/biotechnological drugs along with the most important pharmacological differences and their pre-clinical and clinical development. Moreover, the course will provide detailed information about selected drug classes MANAGEMENT Provide the student with the basis for the study of the mechanisms of action of molecules with pharmacological activity for the development of new molecules, from their identification to the patent. To present, with a focus on life sciences, the major problems related to the management of research projects and the innovations resulting from these, as well as the pathways for exploiting the results, both through technological transfer paths and the start-up of innovative companies. ECONOMY The course aims at providing the students with competences and tools to manage research projects, to start a new company, to work in the bio-pharma industry
PrerequisitesStudents are required to have a good preparation in biochemistry, physiology, anatomy and pathology
Didattics MethodsLectures, Class discussions, Class and homework exercises, Groups Works.
Other informationsFor ECONOMY students are expected to actively participate, including Groups Works Students are required to have a good knowledge of English, especially in reading
Grading rulesMultiple choice test for Pharmacology and Management. ECONOMY: grading is based on one a written exam (70% of the final grade) and one group work (30% of the final grade). The written exam will include open questions and exercises. For groups project students are required to prepare a written document (.doc, max 10 pages) and a PowerPoint presentation (max 15 Slides) by the last class, when findings of the projects will be discussed. If the document is not handled, groups members will be not graded (null grading). The organisation of groups project and minimum requirements for its members will be discussed in class. If a student does not participate in any group, he/she is required to carry out an individual work agreed with the instructor. His/her work (written document, .doc, max 10 pages) should be delivered when he/she takes the written exam. Students are allowed to repeat the exam in any scheduled date after the date the exam has been taken.
Full argumentsMANAGEMENT Topics covered: •    Creativity, scientific results and innovation •    From the idea to the innovation: management of innovative projects and project management tools •   Patent information tools •    Management evaluation and exploitation of scientific results:patents, startups and technology transfer Business model and business plan •    Innovation business development: innovations marketing •    Technology transfer •    Life sciences: industrial structure ECONOMY Introduction to management and basics of economics. Management of biotech inventions - Patents and Intellectual Property Rights (introductory aspects, procedures, economic evaluation of patents) - Technology Transfer: from idea to market. Biotech industry - Introduction to the biotech industry and biotech drugs market - Drugs Research and Development - Drugs Market Regulation - Market access, marketing and sales Economic evaluation of (red) biotechnologies - Cost-effectiveness analysis - Budget Impact analysis PHARMACOLOGY Introduction to pharmacology - Discovery and development of a new drug: pre-clinical and clinical phases - The main principles of drugs, conventional small molecules, biological/biotechnological drugs, equivalent and biosimilar drugs -Pharmacodynamic: receptors and signal transduction; meaning of agonist and antagonist; the dose-response curve; -Pharmacokineti: absorbtion, distribution, metabolism and excretion of drugs; definition of half life, bioavailability, clearance and distribution volume -Antiinflammatory drugs -Immunosuppressive drugs -Chemotherapeutci agents Anti-coagulant and anti-thrombotic drugs - Anti-hypertensive drugs - Drugs of nervous system: anti-depressives, anti-parkinson, anti-psycotic drugs Drug abuse: opioids, psicostimulants, cannabinoids
Expected learning objectivesManaging a biotech invention. Carrying out an economic evaluation of a red biotechnology. Designing and implementing a market access strategy for a biotech medicine. At the end of the course students will have the basis to be able to face a new path of study in the field of pharmacological development
Modules
Course ID Course SSD Teachers
MS0422 Innovation management and technology transfer SECS-P/08 - ECONOMIA E GESTIONE DELLE IMPRESE CONICELLA Fabrizio, FALVO Sara
BM030 Pharmacology BIO/14 - FARMACOLOGIA FRESU Luigia Grazia, TRAVELLI Cristina
M0201 Managerial economics in life sciences SECS-P/07 - ECONOMIA AZIENDALE JOMMI Claudio, SEDDIO Pasquale
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CourseInnovation management and technology transfer
Course IDMS0422
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderCONICELLA Fabrizio
TeachersCONICELLA Fabrizio, FALVO Sara
CFU2
Teaching duration (hours)16
Individual study time 34
SSDSECS-P/08 - ECONOMIA E GESTIONE DELLE IMPRESE
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryC - Affine o integrativo
Year3
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseInnovation management and technology transfer
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersCONICELLA Fabrizio
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageItalian Documents in english
AbstractMain topics covered: Creativity, scientific results and innovation Project management Patent information tools Technology transfer Business model/business plans Marketing
Reference textsSlides and material shared Suggested books: Innovation and Entrepreneurship John Bessant Joe Tidd June 2011 John Wiley & Sons Inc; ISBN-10: 0470711442 ISBN-13: 978-0470711446 Managing Innovation: Integrating Technological, Market and Organizational Change, Joe Tidd, John Bessant March 2009 ISBN-10: 0470998105 ISBN-13: 978-0470998106 http://www.innovation-portal.info La Gestione Del Trasferimento Tecnologico - Strategie, Modelli E Strumenti Andrea Piccaluga, Massimiliano Granieri, Giuseppe Conti 1 edizione (25 agosto 2011) ISBN-10: 884701901X ISBN-13: 978-8847019010 Ricerca e sviluppo nell'industria biotecnologica e farmaceutica Silvano Fumero Bollati Boringhieri (23 maggio 2003) ISBN-10: 8833957071 ISBN-13: 978-8833957074 Economia delle aziende biotecnologiche Franco Angeli; 1 edizione (11 agosto 2015), Collana: Università-Economia ISBN-10: 8891714054 ISBN-13: 978-8891714053 Business Planning Cinzia Parolini : Pearson Collana: Studio & professione Febbraio 2011 ISBN-10: 8871926293 ISBN-13: 978-8871926292 Commercializing Successful Biomedical Technologies: Basic Principles for the Development of Drugs, Diagnostics and Devices Shreefal S. Mehta Cambridge University Press; Reissue edizione (30 aprile 2011) ISBN-10: 0521205859 ISBN-13: 978-0521205856 Building Biotechnology: Biotechnology Business, Regulations, Patents, Law, Policy and Science Yali Friedman Editore: Logos Press; 4. Auflage. edizione (1 gennaio 2014) ISBN-10: 1934899291 Project Management: dall'idea all'attuazione. Una guida pratica per il successo Marion E. Haynes Editore: Franco Angeli (11 ottobre 2012) ISBN-10: 8856811650 ISBN-13: 978-8856811650 ISBN-13: 978-1934899298
Teaching targetsThe Management of Innovation and Technology Transfer aims at presenting, with a focus on life sciences, the major issues related to the management of research projects and the innovations resulting from these, as well as the pathways for enhancing the results, both through technological transfer paths that start up innovative businesses. Particular attention will be devoted to the analysis of the industrial aspects of these pathways and to the patent and commercial exploitation of the results of scientific research, including business start-ups, as well as project management and innovation management issues
PrerequisitesMC004 English
Didattics MethodsPowerPoint presentation and internet access for database consultation. Eventual exercise on the evaluation of a scientific result and identification of exploitation path.
Other informationsThe course objective is to present the key concepts underlying the exploitation of research results with a specific focus on life sciences. The student will acquire basic knowledge of innovation management and technological transfer processes in order to understand the processes underlying the possible exploitation paths of the scientific results and identify and, in perspective, manage, the core elements. The student will also acquire the basic knowledge in order to analyze an entrepreneurial idea and to identify and approach the key components of a business plan. Finally, the student will acquire the ability to analyze, interpret and plan start-up and technology transfer activities and understand the dynamics of the industry.
Grading rulesTest multiple choices
Full argumentsTopics covered: •    Creativity, scientific results and innovation •    From the idea to the innovation: management of innovative projects and project management tools •   Patent information tools •    Management evaluation and exploitation of scientific results:patents, startups and technology transfer Business model and business plan •    Innovation business development: innovations marketing •    Technology transfer •    Life sciences: industrial structure
Expected learning objectivesThe student will acquire basic knowledge of innovation management and technological transfer processes in order to understand the processes underlying the possible exploitation paths of the scientific results and identify and, in perspective, manage, the core elements. The student will also acquire the basic knowledge in order to analyze an entrepreneurial idea and to identify and approach the key components of a business plan. Finally, the student will acquire the ability to analyze, interpret and plan start-up and technology transfer activities and understand the dynamics of the industry.
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CourseInnovation management and technology transfer
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderFALVO Sara
TeachersFALVO Sara
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageItalian
AbstractMain topics covered: Creativity, scientific results and innovation Project management Patent information tools Technology transfer Business model/business plans Marketing
Reference textsSlides and material shared Suggested books: Innovation and Entrepreneurship John Bessant Joe Tidd June 2011 John Wiley & Sons Inc; ISBN-10: 0470711442 ISBN-13: 978-0470711446 Managing Innovation: Integrating Technological, Market and Organizational Change, Joe Tidd, John Bessant March 2009 ISBN-10: 0470998105 ISBN-13: 978-0470998106 http://www.innovation-portal.info La Gestione Del Trasferimento Tecnologico - Strategie, Modelli E Strumenti Andrea Piccaluga, Massimiliano Granieri, Giuseppe Conti 1 edizione (25 agosto 2011) ISBN-10: 884701901X ISBN-13: 978-8847019010 Ricerca e sviluppo nell'industria biotecnologica e farmaceutica Silvano Fumero Bollati Boringhieri (23 maggio 2003) ISBN-10: 8833957071 ISBN-13: 978-8833957074 Economia delle aziende biotecnologiche Franco Angeli; 1 edizione (11 agosto 2015), Collana: Università-Economia ISBN-10: 8891714054 ISBN-13: 978-8891714053 Business Planning Cinzia Parolini : Pearson Collana: Studio & professione Febbraio 2011 ISBN-10: 8871926293 ISBN-13: 978-8871926292 Commercializing Successful Biomedical Technologies: Basic Principles for the Development of Drugs, Diagnostics and Devices Shreefal S. Mehta Cambridge University Press; Reissue edizione (30 aprile 2011) ISBN-10: 0521205859 ISBN-13: 978-0521205856 Building Biotechnology: Biotechnology Business, Regulations, Patents, Law, Policy and Science Yali Friedman Editore: Logos Press; 4. Auflage. edizione (1 gennaio 2014) ISBN-10: 1934899291 Project Management: dall'idea all'attuazione. Una guida pratica per il successo Marion E. Haynes Editore: Franco Angeli (11 ottobre 2012) ISBN-10: 8856811650 ISBN-13: 978-8856811650 ISBN-13: 978-1934899298
Teaching targetsThe Management of Innovation and Technology Transfer aims at presenting, with a focus on life sciences, the major issues related to the management of research projects and the innovations resulting from these, as well as the pathways for enhancing the results, both through technological transfer paths that start up innovative businesses. Particular attention will be devoted to the analysis of the industrial aspects of these pathways and to the patent and commercial exploitation of the results of scientific research, including business start-ups, as well as project management and innovation management issues
PrerequisitesMC004 English
Didattics MethodsPowerPoint presentation and internet access for database consultation. Eventual exercise on the evaluation of a scientific result and identification of exploitation path.
Other informationsThe course objective is to present the key concepts underlying the exploitation of research results with a specific focus on life sciences. The student will acquire basic knowledge of innovation management and technological transfer processes in order to understand the processes underlying the possible exploitation paths of the scientific results and identify and, in perspective, manage, the core elements. The student will also acquire the basic knowledge in order to analyze an entrepreneurial idea and to identify and approach the key components of a business plan. Finally, the student will acquire the ability to analyze, interpret and plan start-up and technology transfer activities and understand the dynamics of the industry.
Grading rulesTest multiple choices
Full argumentsTopics covered: • Creativity, scientific results and innovation • From the idea to the innovation: management of innovative projects and project management tools • Patent information tools • Management evaluation and exploitation of scientific results:patents, startups and technology transfer Business model and business plan • Innovation business development: innovations marketing • Technology transfer • Life sciences: industrial structure
Expected learning objectivesThe student will acquire basic knowledge of innovation management and technological transfer processes in order to understand the processes underlying the possible exploitation paths of the scientific results and identify and, in perspective, manage, the core elements. The student will also acquire the basic knowledge in order to analyze an entrepreneurial idea and to identify and approach the key components of a business plan. Finally, the student will acquire the ability to analyze, interpret and plan start-up and technology transfer activities and understand the dynamics of the industry.
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CoursePharmacology
Course IDBM030
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderFRESU Luigia Grazia
TeachersFRESU Luigia Grazia, TRAVELLI Cristina
CFU5
Teaching duration (hours)40
Individual study time 85
SSDBIO/14 - FARMACOLOGIA
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CoursePharmacology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderFRESU Luigia Grazia
TeachersFRESU Luigia Grazia
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageitalian
AbstractBasic principles of pharmacocynetic and pharmacodynamic. Drugs of nervous system; drugs of cardiovascular system; anti-inflammatory drugs; immunosuppressive drugs; antineoplastic drugs; insulin and oral hypoglicemic drugs
Reference textsThe most recent publications cited during the lectures, the following books are recommended: 1. HP Rang and MM Dale – Farmacologia, Casa Ed Ambrosiana 2. Goodman & Gilman – Le basi farmacologiche della terapia di J.G.Hardman, L.E. Limbird, A.Goodman Gilman, Curatore edizione italiana: C.Sirtori, G.Folco, G.Franceschini, S.Govoni. 3. Farmacologia generale e molecolare, a cura di F.Clementi, G.Fumagalli, Ed UTET
Teaching targetsIn the context of this Integrated Course, the module of Pharmacology will give to students the basis of pharmacokinetic and pharmacodynamic principles, the general concepts of “conventional small molecules” and biological/biotechnological drugs along with the most important pharmacological differences and their pre-clinical and clinical development. Moreover, the course will provide detailed information about selected drug classes
PrerequisitesStudents are required to have a good preparation in biochemistry, physiology, anatomy and pathology
Didattics MethodsLectures with power point presentation
Other informationsStudents are required to have a good knowledge of English, especially in reading
Grading rulesMultiple choice test
Full argumentsIntroduction to pharmacology - Discovery and development of a new drug: pre-clinical and clinical phases - The main principles of drugs, conventional small molecules, biological/biotechnological drugs, equivalent and biosimilar drugs -Pharmacodynamic: receptors and signal transduction; meaning of agonist and antagonist; the dose-response curve; -Pharmacokineti: absorbtion, distribution, metabolism and excretion of drugs; definition of half life, bioavailability, clearance and distribution volume -Antiinflammatory drugs -Immunosuppressive drugs -Chemotherapeutci agents Anti-coagulant and anti-thrombotic drugs - Anti-hypertensive drugs - Drugs of nervous system: anti-depressives, anti-parkinson, anti-psycotic drugs Drug abuse: opioids, psicostimulants, cannabinoids
Expected learning objectivesAt the end of the course students will have the basis to be able to face a new path of study in the field of pharmacological development
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CoursePharmacology
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderTRAVELLI Cristina
TeachersTRAVELLI Cristina
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageitaliano
AbstractBasic principles of pharmacocynetic and pharmacodynamic. Drugs of nervous system; drugs of cardiovascular system; anti-inflammatory drugs; immunosuppressive drugs; antineoplastic drugs; insulin and oral hypoglicemic drugs
Reference textsThe most recent publications cited during the lectures, the following books are recommended: 1. HP Rang and MM Dale – Farmacologia, Casa Ed Ambrosiana 2. Goodman & Gilman – Le basi farmacologiche della terapia di J.G.Hardman, L.E. Limbird, A.Goodman Gilman, Curatore edizione italiana: C.Sirtori, G.Folco, G.Franceschini, S.Govoni. 3. Farmacologia generale e molecolare, a cura di F.Clementi, G.Fumagalli, Ed UTET
Teaching targetsIn the context of this Integrated Course, the module of Pharmacology will give to students the basis of pharmacokinetic and pharmacodynamic principles, the general concepts of “conventional small molecules” and biological/biotechnological drugs along with the most important pharmacological differences and their pre-clinical and clinical development. Moreover, the course will provide detailed information about selected drug classes
PrerequisitesStudents are required to have a good preparation in biochemistry, physiology, anatomy and pathology
Didattics MethodsLectures with power point presentation
Other informationsStudents are required to have a good knowledge of English, especially in reading
Grading rulesMultiple choice test
Full arguments-Introduction to pharmacology - Discovery and development of a new drug: pre-clinical and clinical phases - The main principles of drugs, conventional small molecules, biological/biotechnological drugs, equivalent and biosimilar drugs -Pharmacodynamic: receptors and signal transduction; meaning of agonist and antagonist; the dose-response curve; -Pharmacokineti: absorbtion, distribution, metabolism and excretion of drugs; definition of half life, bioavailability, clearance and distribution volume -Antiinflammatory drugs -Immunosuppressive drugs -Chemotherapeutci agents Anti-coagulant and anti-thrombotic drugs - Anti-hypertensive drugs - Drugs of nervous system: anti-depressives, anti-parkinson, anti-psycotic drugs - Drug abuse: opioids, psicostimulants, cannabinoids
Expected learning objectivesAt the end of the course students will have the basis to be able to face a new path of study in the field of pharmacological development
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CourseManagerial economics in life sciences
Course IDM0201
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderJOMMI Claudio
TeachersSEDDIO Pasquale, JOMMI Claudio
CFU5
Teaching duration (hours)40
Individual study time 85
SSDSECS-P/07 - ECONOMIA AZIENDALE
Course typeModulo di sola Frequenza
Course mandatorietyMandatory course
Course categoryB - Caratterizzante
Year3
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal judgment
Sites and/or partitions
Gruppo A
Gruppo B
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CourseManagerial economics in life sciences
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
TeachersSEDDIO Pasquale, JOMMI Claudio
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageItalian
AbstractIntroduction to management. Management of biotech inventions. Biotech market and industry. Economic evaluation of (red) biotechnologies, with a focus on biotech medicines.
Reference textsThere is not a single textbook. For Management readings will be mentioned during the course. Management of biotech inventions Friedman Y (2013), Building Biotechnology: Biotechnology Business, Regulations, Patents, Law, ThinkBiotech LLC, 4th Edition Bio-pharma industry Gianfrate F, Il mercato dei farmaci tra salute e business, Franco Angeli Editore, 2014 Economic evaluation of red biotechnologies Mennini FS, Cicchetti A, Fattore G, Russo P. La Valutazione Economica dei Programmi Sanitari. Il Pensiero Scientifico ed. 2011. Terza Edizione.
Teaching targetsThe course aims at providing the students with competences and tools to manage research projects, to start a new company, to work in the bio-pharma industry
PrerequisitesNone
Didattics MethodsLectures, Class discussions, Class and homework exercises, Groups Works.
Other informationsStudents are expected to actively participate, including Groups Works
Grading rulesGrading is based on one a written exam (70% of the final grade) and one group work (30% of the final grade). The written exam will include open questions and exercises. For groups project students are required to prepare a written document (.doc, max 10 pages) and a PowerPoint presentation (max 15 Slides) by the last class, when findings of the projects will be discussed. If the document is not handled, groups members will be not graded (null grading). The organisation of groups project and minimum requirements for its members will be discussed in class. If a student does not participate in any group, he/she is required to carry out an individual work agreed with the instructor. His/her work (written document, .doc, max 10 pages) should be delivered when he/she takes the written exam. Students are allowed to repeat the exam in any scheduled date after the date the exam has been taken.
Full argumentsIntroduction to management and basics of economics. Management of biotech inventions - Patents and Intellectual Property Rights (introductory aspects, procedures, economic evaluation of patents) - Technology Transfer: from idea to market. Biotech industry - Introduction to the biotech industry and biotech drugs market - Drugs Research and Development - Drugs Market Regulation - Market access, marketing and sales Economic evaluation of (red) biotechnologies - Cost-effectiveness analysis - Budget Impact analysis
Expected learning objectivesManaging a biotech invention. Carrying out an economic evaluation of a red biotechnology. Designing and implementing a market access strategy for a biotech medicine.
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CourseManagerial economics in life sciences
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderJOMMI Claudio
TeachersSEDDIO Pasquale, JOMMI Claudio
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageItalian
AbstractIntroduction to management. Management of biotech inventions. Biotech market and industry. Economic evaluation of (red) biotechnologies, with a focus on biotech medicines.
Reference textsThere is not a single textbook. For Management readings will be mentioned during the course. Management of biotech inventions Friedman Y (2013), Building Biotechnology: Biotechnology Business, Regulations, Patents, Law, ThinkBiotech LLC, 4th Edition Bio-pharma industry Gianfrate F, Il mercato dei farmaci tra salute e business, Franco Angeli Editore, 2014 Economic evaluation of red biotechnologies Mennini FS, Cicchetti A, Fattore G, Russo P. La Valutazione Economica dei Programmi Sanitari. Il Pensiero Scientifico ed. 2011. Terza Edizione.
Teaching targetsThe course aims at providing the students with competences and tools to manage research projects, to start a new company, to work in the bio-pharma industry
PrerequisitesNone
Didattics MethodsLectures, Class discussions, Class and homework exercises, Groups Works.
Other informationsStudents are expected to actively participate, including Groups Works
Grading rulesGrading is based on one a written exam (70% of the final grade) and one group work (30% of the final grade). The written exam will include open questions and exercises. For groups project students are required to prepare a written document (.doc, max 10 pages) and a PowerPoint presentation (max 15 Slides) by the last class, when findings of the projects will be discussed. If the document is not handled, groups members will be not graded (null grading). The organisation of groups project and minimum requirements for its members will be discussed in class. If a student does not participate in any group, he/she is required to carry out an individual work agreed with the instructor. His/her work (written document, .doc, max 10 pages) should be delivered when he/she takes the written exam. Students are allowed to repeat the exam in any scheduled date after the date the exam has been taken.
Full argumentsIntroduction to management and basics of economics. Management of biotech inventions - Patents and Intellectual Property Rights (introductory aspects, procedures, economic evaluation of patents) - Technology Transfer: from idea to market. Biotech industry - Introduction to the biotech industry and biotech drugs market - Drugs Research and Development - Drugs Market Regulation - Market access, marketing and sales Economic evaluation of (red) biotechnologies - Cost-effectiveness analysis - Budget Impact analysis
Expected learning objectivesManaging a biotech invention. Carrying out an economic evaluation of a red biotechnology. Designing and implementing a market access strategy for a biotech medicine.
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CourseStatistical methods for experimental studies
Course IDMS0446
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMAGNANI Corrado
TeachersMAGNANI Corrado
CFU5
Teaching duration (hours)40
Individual study time 85
SSDMED/01 - STATISTICA MEDICA
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryA - Base
Year3
PeriodPrimo Semestre
Frequenza obbligatoriaYes
Grading typeFinal grade
Sites and/or partitions
Gruppo A
Gruppo B
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CourseStatistical methods for experimental studies
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMAGNANI Corrado
TeachersMAGNANI Corrado
Course typeGruppi
Year3
PartitionGruppo A
Teaching languageItalian
AbstractFirst level course. Statisical methods for data description. Probability. Sampling. Principles of statistical inference. Tests of common use for the analysis of numerical and categorical data.
Reference textsAll the suggested texts are Italian translations of English textbooks. The students can choose the language as they prefer. M.Pagano & K.Gauvreau. Biostatistica (II edizione italiana). ed. Idelson Gnocchi, Napoli 2003. Further readings: - Daniel G Biostatistica. Ed. Edises.. - Armitage & Berry Metodi statistici nella ricerca medica, 3a edizione (ed inglese Blackwell, tradotto e stampato in Italia da McGraw-Hill). The 4th edition is also available in English only. - Siegel & Castellan Statistica non parametrica. McGraw-Hill. – Text on non parametric tests. - Douglas Altman, David Machin, Trevor Bryant, Martin Gardner Statistics with Confidence (2nd ed.) BMJ edition.. - www.publichealth.ac.nz/ Reading of papers on statistical methosds, as those published on British Medical Journal or British Journal of Cancer.
Teaching targetsData description. Probability. Sampling. The student is expected to get the basis for describing data from laboratory activity and for understanding the basic steps of sampling and study design. Understanding of statistical inference. Basic use and interpretation of statistical tests for categorical and numerical data.
PrerequisitesMathematics as in high schools.
Didattics MethodsThe course will include lectures and exercises. For the latter the students will be divided in small groups. Some topics will be left for individual study.
Grading rulesWritten test (60 min approx.) The test includes: -open questions; - exercise solution; - check – list - summary questions on the interpretation of the result of the statistical test. The entire program will be considered in the final test, including the topics left for individual study.
Full arguments1) Presentation General concepts Presentation of the data - Types of data. - Absolute, relative, cumulative frequencies. - Tables of contingency. - Graphical representations of frequency distributions. Chapters of reference: 1 & 2 2) Synthesis and presentation of numerical data Indices of position (mean, median, mode) and of dispersion (standard deviation, coefficient of variation, percentiles). Graphical representations of numerical data. Box plot Geometric mean, logarithmic transformation. Calculation of the average for grouped data. Description of data defined from two variables. - Dot diagrams Chapters of reference: 2 & 3 3) Probability Definition of probability. Calculation of the probability of an event and the combined probability of more events. Conditional probabilities - Application: sensibility, specificity and predictive value of a diagnostic test. Chapters of reference: 6 4)Methods sampling chapter 22 5) Theoretical Distributions of probability Binomial distribution. Calculation of the probability of an event with binomial probability distribution. Normal distribution Standard Normal distribution. Use of the tables of the standard normal distribution. Chapters of reference: 7 5) Sampling and distribution of sample means Definition of population and sample. Reasons for sampling. Relation between population and sample and property of collections of samples. Theorem of the central limit. Applications of the theorem of the central limit. Sample size Chapters of reference: 8 6) The Student t distribution Definition Degrees of freedom. Applications Use of EXCEL statistical functions for the normal distribution and the t distribution. Chapters of reference: 9 Confidence intervals Definition Calculation of the confidence interval for the average, in the cases with known and unknown population variance. Generalization of the calculation of the confidence interval to other statistics. Chapters of reference: 9 7)Test of hypothesis Introduction to the inferential statistics Work Hypothesis and null hypothesis Error of I° and II° type Chapters of reference: 10 8) Comparison between two averages Use of the t-Student Test. Use of the tabulated t distribution and the functions of Excel. - Sample with paired observations. - Independent samples with equal variance. - Confidence interval based on the t distribution. Chapters of reference: 11 9) Analysis of the data in contingency tables Tables of contingency with two lines and two columns (2x2). - Chi-square test. - Association: Odds Ratio and Relative Risk. - Confidence interval of the Odds ratio - Correction of the continuity - Exact test of Fisher - Test of McNemar Extension to tables n x m. Degrees of freedom. Chapters of reference: 15 10) Analysis of the variance - analysis of the variance with one classification criterion Chapters of reference: 11 11) Simple linear regression The regression straight line. The esteem with the least-squares method. Statistical test of regression coefficients. Use of the residuals in order to verify the validity of the assumptions. Confidence intervals for prediction. Reading results of multivariable analysis Chapters of reference: 18 12) Introduction to the design of the study Statistical power Arguments of individual study on the reference text (without lectures) Tests “distribution free” or distribution free General introduction Advantages and disadvantage Comparison with the parametric correspondents tests. Test U di Mann-Whitney Test of Wilcoxon for paired data Chapters of reference: 13 Correlation: Pearson & Spearman (reference: 17)
Expected learning objectivesAims: Basic knowledge for the reading of papers in biotechnology with statistical content. Presentation of data. Interpretation of laboratory results and clinical tests.
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Show parent course details
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CourseStatistical methods for experimental studies
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
Teaching leaderMAGNANI Corrado
TeachersMAGNANI Corrado
Course typeGruppi
Year3
PartitionGruppo B
Teaching languageItalian
AbstractFirst level course. Statisical methods for data description. Probability. Sampling. Principles of statistical inference. Tests of common use for the analysis of numerical and categorical data.
Reference textsAll the suggested texts are Italian translations of English textbooks. The students can choose the language as they prefer. M.Pagano & K.Gauvreau. Biostatistica (II edizione italiana). ed. Idelson Gnocchi, Napoli 2003. Further readings: - Daniel G Biostatistica. Ed. Edises.. - Armitage & Berry Metodi statistici nella ricerca medica, 3a edizione (ed inglese Blackwell, tradotto e stampato in Italia da McGraw-Hill). The 4th edition is also available in English only. - Siegel & Castellan Statistica non parametrica. McGraw-Hill. – Text on non parametric tests. - Douglas Altman, David Machin, Trevor Bryant, Martin Gardner Statistics with Confidence (2nd ed.) BMJ edition.. - www.publichealth.ac.nz/ Reading of papers on statistical methosds, as those published on British Medical Journal or British Journal of Cancer.
Teaching targetsData description. Probability. Sampling. The student is expected to get the basis for describing data from laboratory activity and for understanding the basic steps of sampling and study design. Understanding of statistical inference. Basic use and interpretation of statistical tests for categorical and numerical data.
PrerequisitesMathematics as in high schools.
Didattics MethodsThe course will include lectures and exercises. For the latter the students will be divided in small groups. Some topics will be left for individual study.
Grading rulesWritten test (60 min approx.) The test includes: -open questions; - exercise solution; - check – list - summary questions on the interpretation of the result of the statistical test. The entire program will be considered in the final test, including the topics left for individual study.
Full arguments1) Presentation General concepts Presentation of the data - Types of data. - Absolute, relative, cumulative frequencies. - Tables of contingency. - Graphical representations of frequency distributions. Chapters of reference: 1 & 2 2) Synthesis and presentation of numerical data Indices of position (mean, median, mode) and of dispersion (standard deviation, coefficient of variation, percentiles). Graphical representations of numerical data. Box plot Geometric mean, logarithmic transformation. Calculation of the average for grouped data. Description of data defined from two variables. - Dot diagrams Chapters of reference: 2 & 3 3) Probability Definition of probability. Calculation of the probability of an event and the combined probability of more events. Conditional probabilities - Application: sensibility, specificity and predictive value of a diagnostic test. Chapters of reference: 6 4)Methods sampling chapter 22 5) Theoretical Distributions of probability Binomial distribution. Calculation of the probability of an event with binomial probability distribution. Normal distribution Standard Normal distribution. Use of the tables of the standard normal distribution. Chapters of reference: 7 5) Sampling and distribution of sample means Definition of population and sample. Reasons for sampling. Relation between population and sample and property of collections of samples. Theorem of the central limit. Applications of the theorem of the central limit. Sample size Chapters of reference: 8 6) The Student t distribution Definition Degrees of freedom. Applications Use of EXCEL statistical functions for the normal distribution and the t distribution. Chapters of reference: 9 Confidence intervals Definition Calculation of the confidence interval for the average, in the cases with known and unknown population variance. Generalization of the calculation of the confidence interval to other statistics. Chapters of reference: 9 7)Test of hypothesis Introduction to the inferential statistics Work Hypothesis and null hypothesis Error of I° and II° type Chapters of reference: 10 8) Comparison between two averages Use of the t-Student Test. Use of the tabulated t distribution and the functions of Excel. - Sample with paired observations. - Independent samples with equal variance. - Confidence interval based on the t distribution. Chapters of reference: 11 9) Analysis of the data in contingency tables Tables of contingency with two lines and two columns (2x2). - Chi-square test. - Association: Odds Ratio and Relative Risk. - Confidence interval of the Odds ratio - Correction of the continuity - Exact test of Fisher - Test of McNemar Extension to tables n x m. Degrees of freedom. Chapters of reference: 15 10) Analysis of the variance - analysis of the variance with one classification criterion Chapters of reference: 11 11) Simple linear regression The regression straight line. The esteem with the least-squares method. Statistical test of regression coefficients. Use of the residuals in order to verify the validity of the assumptions. Confidence intervals for prediction. Reading results of multivariable analysis Chapters of reference: 18 12) Introduction to the design of the study Statistical power Arguments of individual study on the reference text (without lectures) Tests “distribution free” or distribution free General introduction Advantages and disadvantage Comparison with the parametric correspondents tests. Test U di Mann-Whitney Test of Wilcoxon for paired data Chapters of reference: 13 Correlation: Pearson & Spearman (reference: 17)
Expected learning objectivesAims: Basic knowledge for the reading of papers in biotechnology with statistical content. Presentation of data. Interpretation of laboratory results and clinical tests.
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CourseTraining
Course IDMS0146
Academic Year2018/2019
Year of rule2016/2017
Degree9415 - BIOTECNOLOGIE
CurriculumCORSO GENERICO
CFU8
Individual study time 200
Course typeAttività formativa monodisciplinare
Course mandatorietyMandatory course
Course categoryF - Stage e altre attività formative
Year3
Frequenza obbligatoriaYes
Grading typeFinal grade
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