Medical Biotechnology

Academic program

Print
Course Molecular diagnostics
Course ID MS0461
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BOLDORINI Renzo Luciano
Teachers Boldorini Renzo Luciano
CFU 11
SSD MED/08 - ANATOMIA PATOLOGICA, BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA, MED/05 - PATOLOGIA CLINICA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 2
Period Secondo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0462Pathologic basis of diseases MED/08 - ANATOMIA PATOLOGICA Boldorini Renzo Luciano
MS0463Clinical applications of Molecular Biology BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA Capello Daniela
MS0464Clinical Pathology MED/05 - PATOLOGIA CLINICA Rolla Roberta
Show parent course details
Print
Course Molecular diagnostics: Pathologic basis of diseases
Course ID MS0462
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BOLDORINI Renzo Luciano
Teachers Boldorini Renzo Luciano
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/08 - ANATOMIA PATOLOGICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Contenuti Diagnostic, prognostic and predictive factors of pathological interest of the main human malignancies: carcinoma of the lung, breast, colorectal, urinary tract, male and female genital, central nervous system, thyroid; major molecular markers of diagnostic, prognostic and predictive interest: technical principles and clinical applications.
Testi di riferimento Kumar, Abbas, Fausto. Robbins and Cotran Pathologic Basis of Disease. VII ed Elsevier, 2005-09-07; scientific articles (references given during the course).
Obiettivi formativi The student must be able to understand the meaning of translational molecular diagnostic procedure in pathological context relating to the main human neoplastic diseases.
Prerequisiti Notions of Pathology, Anatomy and Histology and Molecular Biology.
Metodi didattici Power-point presentations, CD-ROM and educational websites.
Modalità di verifica dell'apprendimento Multiple choice test
Show parent course details
Print
Course Molecular diagnostics: Clinical applications of Molecular Biology
Course ID MS0463
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CAPELLO Daniela
Teachers Capello Daniela
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi At the end of the course students are expected to have acquired a general knowledge about the main molecular strategies utilized for the identification and the analysis of biomarkers in oncology and about the most important molecular targets currently used for cancer diagnosis and therapy
Contenuti OMICS strategies are driving clinical oncology to take a molecular approach to diagnosis, prognostication, and treatment selection. The main objective of the course is providing the basic knowledge for the use of biomarkers in molecular diagnostic and target therapy of cancer
Testi di riferimento Slides; scientific papers discussed during the classes; online public database resources
Obiettivi formativi At the end of the course students will have a general knowledge of 1) the main molecular strategies utilized for the identification and the analysis of biomarkers in oncology: from the genome-wide approaches to the “marker specific”-based methods; 2) the most relevant actionable mutations and biochemical pathways in cancer:
Prerequisiti The student are expected to have a general knowledge of molecular biology and genetics and of the main technologies for genomic investigations
Metodi didattici Slides presented during the course, interactive journal clubs; PDF copy of scientific papers
Altre informazioni All the teaching material and information regarding the course will be available on DIR (https://www.dir.uniupo.it/)
Modalità di verifica dell'apprendimento Evaluation will consist in a written short report on a topic discussed during the course and an oral presentation of a scientific paper with critical discussion. A short, written multiple choice test will allow to evaluate learning concerning the main molecular biology techniques applied to cancer diagnostic.
Programma esteso 1. Molecular strategies utilized for the identification and the analysis of biomarkers in oncology: from the genome-wide approaches to the “marker specific”-based methods2. Mutational landscape of cancer. Definition of drivers and passenger mutations. Classification of tumor driver genes. Strategies for identifying new candidate cancer genes among low frequently mutated genes. The problem of saturation. Mutational heterogeneity in cancer: mutations originated prior tumor initiation and mutations originating during tumor progression. Mutation timer and tumor evolution. Interpatient, intrapatient and intraclonal tumor heterogeneity. Clonal evolution theories. The prospective use of deep sequencing in diagnostic and therapy of cancer.3. Genetic lesions in cancer. Analysis of the most relevant actionable mutations and biochemical pathways in cancer: i) growth factors/tyrosine kinase receptors; ii) PI3K/Akt/mTOR pathway; iii) MAP kinases pathways; iv) cancer stem cells as possible targets in tumor therapy; v) metabolic reprogramming in tumors, hypoxia and neo-angiogenesis; vi) Mechanisms of cancer metastasis: the role of the microenvironment and the role of mutations
Show parent course details
Print
Course Molecular diagnostics: Clinical Pathology
Course ID MS0464
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ROLLA Roberta
Teachers Rolla Roberta
CFU 1
Teaching duration (hours) 4
Individual study time 21
SSD MED/05 - PATOLOGIA CLINICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Contenuti This course provides an overview of the current knowledge in pharmacogenetics.
Testi di riferimento Teaching Material provided by the lecturer. Lecturer's Monograph.
Obiettivi formativi The aim of the present course is to provide knowledge about the influence of genetics on drug therapy and about the validity and utility of pharmacogenomic tests in the clinical setting.
Prerequisiti Thorough knowledge of Molecular Biology and Genetics (basics of genomics as well as methods to identify mutations).
Metodi didattici PowePoint presentation. Teaching Network on DIR Website. Frontal lessons.
Modalità di verifica dell'apprendimento Multiple choice test
Programma esteso One of the great challenges in drug development and therapy is maximizing therapeutic response while avoiding adverse effects. Advances in genetic knowledge, gained through sequencing, have identified heritable genetic variants that predict drug response. Pharmacogenetics is the study of inherited genetic differences in drug metabolic pathways which can affect individual responses to drugs, both in terms of therapeutic effect as well as adverse effects/ toxicity. The use of these genetic informations can play a major role in clinical practice to predict the right choice and dose of medications for individuals, thus providing a scientific basic for “personalized medicine”. This is particularly important in the case of anticancer drugs whose the therapeutic index is very narrow and a large proportion of patients do not respond. Although the knowledge on an individual’s DNA sequence as an integral determinant of drug therapy has not yet become standard clinical practice, however several genetics recommendations/guidelines for physicians have been developed and are highlighted in the present course. In particular, the course explores the pharmacogenetic clinical utility in: •anticancer drugs (tamoxifen & breast cancer) •treatment of chronic pain (codeine) •antipsychotic therapy •antiplatelet therapy (clopidogrel) •oral anticoagulant therapy (warfarin)
Print
Course Transplantation and Biocompatibility
Course ID MS0480
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader RIMONDINI Lia
Teachers Rimondini Lia
CFU 7
SSD MED/15 - MALATTIE DEL SANGUE, MED/14 - NEFROLOGIA, MED/04 - PATOLOGIA GENERALE, BIO/16 - ANATOMIA UMANA, MED/28 - MALATTIE ODONTOSTOMATOLOGICHE, BIO/11 - BIOLOGIA MOLECOLARE
Course type Attività formativa integrata
Course mandatoriety OBB
Year 2
Period Primo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0481Hematopoietic stem cell biology and transplantation MED/15 - MALATTIE DEL SANGUE Gaidano Gianluca
MS0482Biotechnology in kidney transplantation MED/14 - NEFROLOGIA Quaglia Marco
MS0483Transplant immunology MED/04 - PATOLOGIA GENERALE Chiocchetti Annalisa
MS0484Materials Biocompatibility BIO/16 - ANATOMIA UMANA Boccafoschi Francesca
MS0485Nanomaterials MED/28 - MALATTIE ODONTOSTOMATOLOGICHE Rimondini Lia
MS0493Molecular strategies for improving materials biocompatibility BIO/11 - BIOLOGIA MOLECOLARE Mantovani Diego
Show parent course details
Print
Course Transplantation and Biocompatibility: Hematopoietic stem cell biology and transplantation
Course ID MS0481
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GAIDANO Gianluca
Teachers Gaidano Gianluca
CFU 1
Teaching duration (hours) 6
Individual study time 19
SSD MED/15 - MALATTIE DEL SANGUE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento The course will be given in English
Risultati di apprendimento attesi At the end of the course, the student will be able to critically analyse the strategies of transplant procedures of hematopoietic stem cells and their application in hematology
Contenuti The course will deal with hematopoietic stem cell transplantation, both autologous and allogeneic, with a particular focus on the aspects that are relevant for Medical Biotechnologists.
Testi di riferimento Testo per la consultazione: Williams Hematology, 9e Author(s): Kenneth Kaushansky, Marshall A. Lichtman, Josef T. Prchal, Marcel M. Levi, Oliver W. Press, Linda J. Burns and Michael Caligiuri (available on UPO off campus library via AccessMedicine)
Obiettivi formativi The aim of the course is to render the student able to know, interpret and analyse the main hematological topics of the course, that are are relevant for a Medical Biotechnologist, with a critical attitude, as requested by the Dublin descriptor 3.
Prerequisiti Knowledge of main blood disorders and of immunology
Metodi didattici A) Taught (frontal) classes with slide presentations available on the UPO DIR website (https://www.dir.uniupo.it/); B) Self-assessment tests after each class
Altre informazioni Participation in the self-assessment activitities after each class is strongly recommended
Modalità di verifica dell'apprendimento The exam will be in the context of the exam of the integrated course. The exam of Hematopoietic Stem Cell Biology and Transplantation will be based on 3 statements, for which the candidate has to: i) assign true/false; ii) write 5-10 lines of comment to justify the choice. Examples of these tests are provided as self-assessment tests after each class.
Programma esteso A. The hematopoietic stem cell B. Hematopoiesis C. General concepts of hematopoietic stem cell transplant D. Autologous stem cell transplant E. Allogeneic and syngeic stem cell transplant F. Graft versus host disease and graft verus tumor effect
Show parent course details
Print
Course Transplantation and Biocompatibility: Biotechnology in kidney transplantation
Course ID MS0482
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader QUAGLIA Marco
Teachers Quaglia Marco
CFU 1
Teaching duration (hours) 6
Individual study time 19
SSD MED/14 - NEFROLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi General knowledge of main topics of Nephrology, Dialysis and Transplantation with a focus on biotechnological applications.
Contenuti The course aims at providing general knowledge about management of nephropathic patient, including patient with chronic kidney disease (CKD), on dialysis and with renal transplant, with a focus on modern therapies and diagnostic tools of renal disease and on attendant biotechnological aspects. The following topics will be covered in detail: Assessment of renal function and urinalysis Diagnostic tools in Nephrology including renal biopsy Nephrological syndromes and main renal disorders Chronic kidney disease (CKD) and acute kidney injury (AKI) Artificial renal replacement therapy (RRT): hemodialysis and peritoneal dialysis. Biotechnological applications in dialysis. Naturall renal replacement therapy (RRT): renal transplant. Principles of immunosuppressive therapy. Biotechnological applications in renal transplant.
Testi di riferimento The slides presented during formal lessons represent the reference material for final exam. For further study, main textbooks of Nephrology and selected review articles are available for consultation at the Nephrology and Renal Transplant Unit.
Obiettivi formativi 1) knowledge and understanding of main pathophysiological mechanisms of renal disease; 2) knowledge and understanding of main renal disorders and attendant clinical and laboratory manifestations; 3) knowledge and understanding of principles of therapy of renal disorders, with a focus on artificial and natural renal replacement therapy (RRT) and relative biotechnological applications 4) knowledge and understanding of clinical problems of nephropathic patient, including patient with advanced chronic kidney disease (CKD), patient on dialysis and with renal transplant and relative biotechnological applications 5) development of autonomous judging skills and of adequate communicative skills, (correct medical language) 6) development of autonomous learning skills towards main topics of Nephrology.
Prerequisiti Student must be familiar with basicl principles of anatomy and renal physiology
Metodi didattici The course is based on 3 lessonswhich deal with topics described in the program. Within each lesson attendant biotechnological aspects are highlighted. Students are offered the possibility to explore biotechnological aspects of their interest through direct involvement in laboratory activities. Teaching tools are Videoprojector and PCs with Internet connection, which can be found in the Nephrology and Transplant Unit, where a Nephrological library and main texts and nephrological reviews are also available for further study.
Altre informazioni Prof Marco Quaglia is always available for any further information or request at the Nephrology and Transplant Unit of "Maggiore della Carità" Hospital in Novara (0321-3733786).
Modalità di verifica dell'apprendimento Final exam is based on a written proof which contains "statements". The student is required to judge them (true/false) and to comment on them motivating his judgement. "Statements"are about general topics of Nephrology, Dialysis and Transplantation with biotechnological implications.
Programma esteso Principles of anatomy and renal physiology. Basis of renal semeiotics. Urinalysis and urinary sediment. Study of renal function. Renal ultrasound and other instrumental exams to study renal disorders. Renal biopsy. The nephrological syndromes: isolated urinary abnormalities, nephrotic syndrome, acute nephritic syndrome, rapidly progressive syndrome; acute kidney injury (AKI) and chronic kidney disease (CKD). Primary and secondary glomerulonephritis. Acute and chronic interstitial nephritis. Acuta pyelonephritis. Vascular nephropathies. Acute kidney injury (AKI). Chronic kidney disease (CKD). Progression of chronic renal failure. Uremic syndrome. Preparation for dialysis. Hemodialysis: general principles and indications. Arterial-venous fistula for hemodialysis. Management of hemodialysis patient. Peritoneal dialysis: general principles and indications. Management of hemodialysis patient. Renal transplant. General principles and indications. Management of renal transplant patient. The immunosuppressive therapy. Complications of renal transplant. The role of C4d in the diagnosis of antibody-mediated rejection.
Show parent course details
Print
Course Transplantation and Biocompatibility: Transplant immunology
Course ID MS0483
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CHIOCCHETTI Annalisa
Teachers Chiocchetti Annalisa
CFU 2
Teaching duration (hours) 8
Individual study time 42
SSD MED/04 - PATOLOGIA GENERALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Show parent course details
Print
Course Transplantation and Biocompatibility: Materials Biocompatibility
Course ID MS0484
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BOCCAFOSCHI FRANCESCA
Teachers Boccafoschi Francesca
CFU 1
Teaching duration (hours) 4
Individual study time 21
SSD BIO/16 - ANATOMIA UMANA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Testi di riferimento Teaching material furnished via DIR
Obiettivi formativi Provide the basis for understanding the biocompatibility of materials for biomedical use
Prerequisiti Cell biology, immunology, molecular biology
Metodi didattici Lectures in class
Modalità di verifica dell'apprendimento Written exam
Programma esteso 1.Biocompatibility: basic concepts 2. Polymers: biomechanical characteristics
Show parent course details
Print
Course Transplantation and Biocompatibility: Nanomaterials
Course ID MS0485
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader RIMONDINI Lia
Teachers Rimondini Lia
CFU 1
Teaching duration (hours) 4
Individual study time 21
SSD MED/28 - MALATTIE ODONTOSTOMATOLOGICHE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi Knowledge and understanding of the main properties of matter at nanoscale and the potential application in Medicine Knowledge of the main methods to manage and characterize matter at the nanoscale
Contenuti See Course program beloiw
Testi di riferimento Will be provided during classes
Obiettivi formativi Knowledge and understanding of the main properties of the matter at the nanoscale and their characterization. Knowledge and understanding of the main properties of the nanomaterials used in Medicine and their applications. Development of skills addressed to plan R&D on nanomaterials for Medical applications.
Prerequisiti Fundamentals of chimics and physics
Metodi didattici Lecture
Altre informazioni None
Modalità di verifica dell'apprendimento Written test: Statments assessment and comments
Programma esteso Definition of nanomaterials; properties of the materials at the nanoscale; methods to characterized nanomaterials; methods for nanomaterials manufacturing; esample of nanomaterials used for diagnosis and treatment of various diseases (including cancer); nanocarriers for drugs, nanomaterials for tissue regeneration, toxicology of nanomaterials.
Show parent course details
Print
Course Transplantation and Biocompatibility: Molecular strategies for improving materials biocompatibility
Course ID MS0493
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader MANTOVANI DIEGO
Teachers Mantovani Diego
CFU 1
Teaching duration (hours) 8
Individual study time 17
SSD BIO/11 - BIOLOGIA MOLECOLARE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Primo Semestre
Grading type G
Print
Course Public health and regulatory aspects
Course ID MS0473
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FAGGIANO Fabrizio
Teachers Faggiano Fabrizio
CFU 11
SSD MED/42 - IGIENE GENERALE E APPLICATA, SECS-P/07 - ECONOMIA AZIENDALE, MED/01 - STATISTICA MEDICA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 2
Period Primo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0474Public Health MED/42 - IGIENE GENERALE E APPLICATA Faggiano Fabrizio
MS0475Innovation and Patents SECS-P/07 - ECONOMIA AZIENDALE Gattei Valter
MS0476Biomedical statistics MED/01 - STATISTICA MEDICA Magnani Corrado
Show parent course details
Print
Course Public health and regulatory aspects: Public Health
Course ID MS0474
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FAGGIANO Fabrizio
Teachers Faggiano Fabrizio
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD MED/42 - IGIENE GENERALE E APPLICATA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Show parent course details
Print
Course Public health and regulatory aspects: Innovation and Patents
Course ID MS0475
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GATTEI VALTER
Teachers Gattei Valter
CFU 1
Teaching duration (hours) 8
Individual study time 17
SSD SECS-P/07 - ECONOMIA AZIENDALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Show parent course details
Print
Course Public health and regulatory aspects: Biomedical statistics
Course ID MS0476
Academic Year 2017/2018
Year of rule 2016/2017
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader MAGNANI Corrado
Teachers Magnani Corrado
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/01 - STATISTICA MEDICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi At the end students are expected to plan an experimental study and to conduct basic analyses of numerical and categorical data.
Contenuti Statistical inference, design of experimental study, methods of statistical analysis
Testi di riferimento Online Statistics Education: An Interactive Multimedia Course of Study (available at: onlinestatbook.com)In English-Triola & Triola. Biostatistics. Pearson International Ed-Rosner Fundamentals of Biostatistics. Thomson Books In Italian:- Pagano e Gauvreau Biostatistica, II ed. Idelson - Daniel WW Biostatistica Edises 2000Further redings:•Armitage & Berry Metodi statistici nella ricerca medica, 3a edizione McGraw-Hill (In English Statistical Methods in Medical Research, 4th Edition. Peter Armitage, Geoffrey Berry, J. N. S. Matthews. Wiley ed). •Siegel & Castellan Statistica non parametrica. McGraw-Hill. (In English Nonparametric Statistics for the Behavioral Sciences, II ed. Mc Graw Hill)•Altman, Machin, Bryant & Gardner Statistica medica - Intervalli di confidenza nella ricerca biomedica. Minerva Medica 2004 (In English Statistics with confidence, Ed BMJ Books)•Bossi, Cortinovis, Duca, Marubini Introduzione alla Statistica medica Nuova Italia Scientifica.Further teaching material will be given or indicated during the course.
Obiettivi formativi It is an upper level course, for students who already undertook a first level statistics course.At the end students are expected to plan an experimental study and to conduct basic analyses of numerical and categorical data.
Prerequisiti Basic knowledge in mathematics and statistics, corresponding to the programns in high school (mathematics) and in three years degree in biotechnology (statistics).
Metodi didattici Lectures and exercises
Altre informazioni -
Modalità di verifica dell'apprendimento Written test, including solution of numerical problems and interpretation of results.
Programma esteso Sampling, inference, statistical testing of hypothesis (test of hypothesis) and confidence intervalIntroduction to the experimental designStatistical powerANOVA (Analysis of variance ) one level of classificationLinear regression analysis Analysis of cross-classified data
Print
Course Regenerative Medicine
Course ID MS0491
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FOLLENZI Antonia
Teachers Follenzi Antonia
CFU 10
SSD BIO/11 - BIOLOGIA MOLECOLARE, BIO/17 - ISTOLOGIA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Secondo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0492Molecular Biology BIO/11 - BIOLOGIA MOLECOLARE Pelicci Giuliana
MS0479Histology, Gene and Cell Therapy BIO/17 - ISTOLOGIA Follenzi Antonia
Show parent course details
Print
Course Regenerative Medicine: Molecular Biology
Course ID MS0492
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader PELICCI Giuliana
Teachers Pelicci Giuliana
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD BIO/11 - BIOLOGIA MOLECOLARE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Grading type G
Lingua insegnamento English
Contenuti Review of general topics of Molecular Biology relevant to the understanding of the course. Chromatin Remodeling: Histone Modifications, DNA methylation Structural classes of transcription factors (Myc, Fos) Epigenetic definition: epigenetic diseases and epigenetic therapies. Oncogenes and tumor suppressor genes. P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function. Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions. Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells. Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep). Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency. Parallelisms between epigenetic reprogramming and cancer. Therapeutic use of iPS Cells and embryonic stem cells.
Testi di riferimento Molecular Biology of the Cell Alberts Scientific Papers
Obiettivi formativi Provide basic knowledge of stem cell biology, reprogramming and pluripotency with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.
Prerequisiti A basic understanding of cell biology and molecular biology.
Metodi didattici PowerPoint presentations of lessons and articles provided by the lecturer
Modalità di verifica dell'apprendimento Written Examination and oral presentation of a scientific journal article.
Programma esteso Review of general topics of Molecular Biology relevant to the understanding of the course. Chromatin Remodeling: Histone Modifications, DNA methylation Structural classes of transcription factors (Myc, Fos) Epigenetic definition: epigenetic diseases and epigenetic therapies. Oncogenes and tumor suppressor genes. P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function. Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions. Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells. Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep). Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency. Parallelisms between epigenetic reprogramming and cancer. Therapeutic use of iPS Cells and embryonic stem cells.
Show parent course details
Print
Course Regenerative Medicine: Histology, Gene and Cell Therapy
Course ID MS0479
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FOLLENZI Antonia
Teachers Follenzi Antonia
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD BIO/17 - ISTOLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Grading type G
Print
Course Functional Genomics
Course ID MS0455
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GIORDANO Mara
Teachers Giordano Mara
CFU 11
SSD MED/03 - GENETICA MEDICA, BIO/10 - BIOCHIMICA, BIO/18 - GENETICA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Primo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0456Genomic analysis MED/03 - GENETICA MEDICA Giordano Mara
MS0457Regulation of gene expression BIO/10 - BIOCHIMICA Baldanzi Gianluca
MS0458Genetics BIO/18 - GENETICA Zucchelli Silvia
Show parent course details
Print
Course Functional Genomics: Genomic analysis
Course ID MS0456
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GIORDANO Mara
Teachers Giordano Mara
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD MED/03 - GENETICA MEDICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi Knowledge of the structure of the genome. Acquisition of the main genomic analysis methods. Analysis of databases. Understanding of genomics articles
Contenuti Human Genome Project. Structure and Evolution of the Human Genome. Methods of studying monogenic and multifactorial diseases. Analysis of genomic rearrangements by CGH array. Next Generation Sequencing Techniques (NGS). Genomic imprintig. Methods for studying epigenetic variations in the Human genome.
Testi di riferimento Articles in Juornals chosen by the Professor
Obiettivi formativi The course aims to illustrate the main approaches of genomic analysis. The first part of the course will be used to understand the structure and evolution of the genome that are the main requirements and then move on to genomic analysis methods. Students will then learn to apply DNA-based microarrays and next-generation sequencing technologies, addressing their problems and understanding their perspectives.
Prerequisiti The student is supposed to know the basic genetics and the modalities of transmission of Mendelian diseases. Moreovere the students must know the main molecular genetic techniques.
Metodi didattici Frontal lessons with slides. Student seminars as an in-depth study of topics covered in class. Tutorials through interactive quizzes. Exercises in the computer classroom
Modalità di verifica dell'apprendimento Written exam with open questions Oral examination
Programma esteso Human Genome Project: themain stages of the project and the methodology Structure and Evolution of the Human Genome. Chromatin and chromosome structure. Transposable elements in the genome. Transposons function during evolution. Repeated sequences in the genome. Function of Copy Number Variations. Genome analysis. Methods of studying monogenic and multifactorial diseases. Analysis of genomic rearrangements by CGH array. New Generation Sequencing Techniques (NGS). Genome sequencing. Exome sequencing. Sequencing of the transcriptome. Genomic imprintig. Methods for studying epigenetic variations in the Human genome. Analysis of genomic methylation. Identification of genes that cause genetic diseases in humans through NGS. Study of multifactorial diseases by GWAS (Genome wide association study).
Show parent course details
Print
Course Functional Genomics: Regulation of gene expression
Course ID MS0457
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BALDANZI GIANLUCA
Teachers Baldanzi Gianluca
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD BIO/10 - BIOCHIMICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi Understanding the mechanisms of regulation of gene expression and how such knowledge can be used in research or therapy.
Contenuti After a quick review of gene structure in eukaryotes and prokaryotes, the issue of gene expression regulation will be presented. The first part of the course will deal with prokaryotes gene expression and its regulation. A series of examples will illustrate the possibility of engineering promoters and genes for biotechnological purposes. The second part of the course will deal with the eukaryotes gene expression and the multiple levels of its regulation. Issues: 1) chromatin organization and structure, 2) transcription factor function, 3) pre-initiation complex, 4) transcription termination, 5) RNA stability and 6) RNA translation. The third part of the course will deal with the mechanisms by which extracellular signals and intracellular cues regulates gene expression in eukaryotes. A particular emphasis will be placed on the alterations of gene expression in cancer and on the regulation of gene expression in function of nutrients and metabolic status of the cell.
Testi di riferimento For the improvement of students background Harper's Illustrated Biochemistry, 29e, Robert K. Murray, David A. Bender, Kathleen M. Botham, Peter J. Kennelly, Victor W. Rodwell, P. Anthony Weil Please note: available on Access Medicine for our students Molecular Biology of the Cell - Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter Edition:5th Please note: the 4th edition is available for free on NCBI bookshelf http://www.ncbi.nlm.nih.gov/books/NBK21054/ Suggested textbook Genomes 3 By Terence A. Brown Please note: the 2nd edition is available for free on NCBI bookshelf http://www.ncbi.nlm.nih.gov/books/NBK21128/ Cell Signalling Biology Professor Sir Michael Berridge http://www.biochemj.org/csb/ An extended series of reviews evidenced during the course.
Obiettivi formativi Improving background and learning problem solving in the study of gene expression.
Prerequisiti • Basic Biology: Prokaryotes and Eukaryotes intracellular organization. • Basic biochemistry: DNA and RNA structure and metabolism, protein synthesis, enzyme proprieties. • Basic molecular biology: concept of gene, gene expression, promoter, transcription factor, RNA synthesis and degradation. For the students lacking a strong background in life sciences a series of readings will be proposed to improve their skills on those topics.
Metodi didattici During the course, slides and webinars are extensively used. A copy of the slides and the websites used during the course will be available on the DIR site. Students will undertake teacher driven research/presentation/teamwork.
Modalità di verifica dell'apprendimento Written exam on a series of topics evidenced during the course.
Programma esteso Topic 1 Gene expression regulation Recall of the organization of genes in eukaryotes (enhancers, promoters, transcription start site, introns/exons, transcription termination sites). Control of gene transcription at the level of transcription initiation rate, splicing and alternative splicing, mRNA stability, RNA interference and microRNA. Transcription factors organization: structure of DNA binding and trans-activating domains. Major DNA regulatory sequences. Assembly of RNApolII transcription initiation complex. Methods to study DNA regulatory elements, Promoter structure and transcription factor binding sites. Transcription factors and chromatin structure, chromatin remodeling and gene expression. Post transcriptional modification of histones and the histone code. DNA mutilation. Other ways to regulate gene expression: alternative promoters, alternative splicing, control of mRNA stability, control of mRNA translation, miRNA and RNA interference. Topic 2 Control of gene expression by extracellular and intracellular cues. How signal transduction pathways regulates gene expression: - The nuclear receptor family - The STAT family - ERK signaling to the serum response elements. Topic 3 Transcription factors mutated in tumors. - Myc amplification in growth factors signaling and tumor formation - Hif in the response to hypoxia and tumorigenesis - P53 mutations, Case study: How oncogenes and altered signaling pathways regulates tumor cell metabolism. Peculiarities of tumor metabolism, the Warburg effect and beyond. Methods to study metabolism, integrating gene expression, metabolomics and in vivo data. Use of mass spectrometry, PET/NMR probes, and electrodes (Oxygen Consumption Rate – ExtraCellular Acidification Rate) to study metabolism in living cells/tissues/patients. Experimental manipulation of metabolism and possible routes for cancer therapy. Tumor metabolomics, onco-metabolites and the peculiarities of tumor metabolism. How alterations of signal transduction machinery and gene expression integrates with a peculiar micro-environment to dictate tumor metabolism. How alterations of the metabolism (i.e. dietary habits or cell energetic status) influence chromatin status and gene expression.
Show parent course details
Print
Course Functional Genomics: Genetics
Course ID MS0458
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ZUCCHELLI Silvia
Teachers Zucchelli Silvia
CFU 1
Teaching duration (hours) 8
Individual study time 17
SSD BIO/18 - GENETICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Print
Course Neoplastic and proliferative disorders
Course ID MS0469
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ISIDORO Ciro
Teachers Isidoro Ciro
CFU 12
SSD MED/15 - MALATTIE DEL SANGUE, MED/04 - PATOLOGIA GENERALE
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Primo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi refer to MS0470 and MS0471
Contenuti refer to MS0470 and MS0471
Testi di riferimento refer to MS0470 and MS0471
Obiettivi formativi refer to MS0470 and MS0471
Prerequisiti refer to MS0470 and MS0471
Metodi didattici refer to MS0470 and MS0471
Altre informazioni refer to MS0470 and MS0471
Modalità di verifica dell'apprendimento refer to MS0470 and MS0471
Programma esteso refer to MS0470 and MS0471
Modules
Course ID Course SSD Teachers Agenda web
MS0470Hematoncology MED/15 - MALATTIE DEL SANGUE Gaidano Gianluca
MS0471Experimental oncology MED/04 - PATOLOGIA GENERALE Isidoro Ciro
Show parent course details
Print
Course Neoplastic and proliferative disorders: Hematoncology
Course ID MS0470
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GAIDANO Gianluca
Teachers Gaidano Gianluca
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/15 - MALATTIE DEL SANGUE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento The course is given in English
Risultati di apprendimento attesi At the end of the course, the student will be able to: i) critically analyse the molecular pathology of several models of hematologic malignancies; ii) appreciate the importance of molecular pathology for improving precision medicine, molecular diagnosis and target treatment in hematologic neoplasia
Contenuti The course will deal with the molecular pathology of the main haematological malignancies, especially by analysing the topics relevant for the profession of Medical Biotechnologist. During the course, self-evaluation exercises will also be proposed, as well as additional material for in-depth study and group presentations on topics of particular interest.
Testi di riferimento Reference textbook: Williams Hematology, 9e Author(s): Kenneth Kaushansky, Marshall A. Lichtman, Josef T. Prchal, Marcel M. Levi, Oliver W. Press, Linda J. Burns and Michael Caligiuri (available on UPO off campus library via AccessMedicine)
Obiettivi formativi The aim of the course is to render the student able to know, interpret and analyse the main hematological topics of the course, that are are relevant for a Medical Biotechnologist, with a critical attitude, as requested by the Dublin descriptor 3.
Prerequisiti Basic knowledge of cell and molecular biology
Metodi didattici A) Taught (frontal) classes with slide presentations available on the UPO DIR website (https://www.dir.uniupo.it/); B) Self-assessment tests after each class; C) Group presentations by the students on topics agreed upon with the professor
Altre informazioni Participation in the self-assessment activitities after each class is strongly recommended
Modalità di verifica dell'apprendimento The exam will be in the context of the exam of the integrated course. The exam of Hematoncology will be based on 4 statements, for which the candidate has to: i) assign true/false; ii) write 5-10 lines of comment to justify the choice. Examples of these tests are provided as self-assessment tests after each class.
Programma esteso A. Hematopoiesis and hematopoietic stem cell B. General concepts of myeloid neoplasia and chronic myeloid leukemia: general concepts, molecular pathology, molecular diagnostics and target treatment C. BCR/ABL negative myeloproliferative neoplasia (MPN): general concepts, molecular pathology, molecular diagnostics and target treatment D. General concepts on lymphoid neoplasia, Analysis of B-cell clonality, and Mantle cell lymphoma: general concepts, molecular pathology, molecular diagnostics and target treatment E. Follicular lymphoma: general concepts, molecular pathology, molecular diagnostics and target treatment F. Hairy cell leukemia and Burkitt lymphoma: general concepts, molecular pathology, molecular diagnostics and target treatment G. Lymphoplasmacytic lymphoma and Splenic marginal zone lymphoma: general concepts, molecular pathology, molecular diagnostics and target treatment H. Chronic lymphocytic leukemia: general concepts, molecular pathology, molecular diagnostics and target treatment I. Acute lymphoblastic leukemia and CAR T-cells: general concepts, molecular pathology, molecular diagnostics and target treatment J. Minimal residual disease (MRD); Precision Medicine, Clinical Trials and Benefit Sharing in Hematoncology
Show parent course details
Print
Course Neoplastic and proliferative disorders: Experimental oncology
Course ID MS0471
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ISIDORO Ciro
Teachers Isidoro Ciro
CFU 7
Teaching duration (hours) 28
Individual study time 147
SSD MED/04 - PATOLOGIA GENERALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi Knowing the causes and mechanisms of carcinogenesis and tumor progression. Knowing how to interpret, discuss and present the experimental laboratory results or found in literature, specifically aimed at describing and understanding the neoplastic transformation and behavior of tumor cells.
Contenuti 1. the epidemiologic relevance of Cancer; the influence of the environment and of the life style. 2. the main histopathologic characteristics of the tumours and their classification 3. the molecular alterations responsible for the malignant behaviour of tumour cells (loss of proliferative control; resistance to apoptosis; invasiveness; altered metabolism of glucose, aminoacids and fatty acids; altered proteome homeostasis); the role of autophagy and of cell death in cancer 4. the role of genetics and epigenetics in cancer development and progression; the genetic defects that predispose to cancer; genome (chromosomal translocation) alterations and gene mutations (oncogenes, oncosuppressors) associated with cancer 5. the origin and phenotypic characteristics of Cancer Stem Cells; the genetic and metabolic re-programming leading to cancer 6. the mechanisms of metastasis formation; the role of proteolytic enzymes 7. the inter-relationship between tumour cells and the tumour stroma, with particular regard to the role of cancer associated fibroblasts and of immune cells; 8. the molecular and patho-physiological mechanisms responsible for the cachexia syndrome. 9. the current techniques for Diagnosis (imaging, biomarkers) and the therapeutic treatments available (chemical chemotherapy, biomolecular therapy). 10. Novel therapeutic approach (epigenetics); novel paradigms for carcinogenesis (microbioma);
Testi di riferimento The Molecular Biology of Cancer S-Pelengaris and M. Khan (Wiley-Blackwell edition) Chapter 7 NEOPLASIA by Thomas P Stricker and V Kumar (from Robbins, The molecular Basis of Disease) USEFUL WEB SITES: http://www.insidecancer.org; http://www.nci.nih.gov/; http://www.oncolink.com/; http://www.cancer.gov; http://www.medlib.med.utah.edu/WebPath/LIVEHTML/LIVERIDX.html#5 http://www.ncbi.nlm.nih.gov/books/bv.fcgi?call=bv.View..ShowSection&rid=gnd.chapter.10
Obiettivi formativi  How to recognize the histopathological characteristics of major human neoplasms;  Understand what molecular and cellular alterations determine the neoplastic phenotype;  Considering the natural causes of carcinogenesis and the factors that can cause tumor recurrence, bearing in mind the role of defensive systems at the molecular, cellular and organizational levels;  Describe the distinctive (morphological, metabolic, behavioral) characteristics of neoplastic stem cells;  Know the mechanisms responsible for the infiltration and metastasis of tumors;  illustrate how the stromal microenvironment surrounding the tumor is modified and how can this promote neoplastic growth  Recognize clinical signs and know how to describe the molecular and pathophysiological mechanisms of neoplastic cachexia. KNOWLEDGE AND ABILITY EXPECTED At the end of the course, the student should be able to read and analyse scientific articles on cellular and molecular biology of the tumours (during the course each student will have to present a scientific article on a subject). Learning Outcomes: Knowledge about the cellular and molecular mechanisms underlying the cancerogenesis process. Dynamics of neoplastic populations. Natural History of Neoplasms. Risk Factors for the Development of Neoplasms.
Prerequisiti Basic Knowledge of Cell Biology and Histology, Molecular Biology and Biochemistry.
Metodi didattici Interactive lesson involving students continuously with questions to stimulate curiosity and test understanding of concepts. Illustration of processes with practical examples. Discussion of relevant scientific articles. A copy of the slides presented during the lessons will be provided in advance
Altre informazioni Students can contact the teacher and ask for explanations after appointment by email. OPTIONAL ACTIVITIES: Students are welcome to spend a period in the laboratory of Molecular Pathology to participate in the design, execution and discussion of experiments. CFU will be assigned based on the duration of the stage.
Modalità di verifica dell'apprendimento EXAM MODALITY (2 steps for each module) 1. Written test (30 minutes for 3 statements): answer TRUE/FALSE and give an explanation to support your answer in 5 (not less) to10 (not more) lines. (please, write in a legible writing). At least 2 out of 3 answers must be correct in order to be admitted to the next step (oral examination). (Evaluation: 6-8/30 points for each question). 2. Oral examination (approx. 10-20 min): A) discussion of the written test. B) discussion of an original articles dealing with a subject of your choice inherent the program of the course and published in a good journal (consult with your professor for an appropriate choice). (Evaluation: 4-6/30 points). C) (optional): 1 or 2 extra question(s). (Evaluation 2-4/30, Laude). Maximal score is 30 + Laude/30. 3. NOTE: 1. the final score is agreed between the two Professors as an average of the scores obtained for the two modules. 2. in case only one of the two modules (A or B) is passed (score >18/30), the vote will be considered valid for only the next session in which the other module (B or A) must be passed. In case of failure the student must repeat the exam for both (A and B) modules in a new session.
Programma esteso Introduction to Cancer: Definition, Epidemiology, Origin of cancer (multistage carcinogenesis, genetic and epigenetic clonal expansion; mutations, risk factors). Nomenclature, Histopathology. Classification, Staging, Grading. Aetiology; Gene – environment interaction; chemical carcinogenesis; radiation and carcinogenesis; virus and carcinogenesis. Life style and Cancer (smoke, diet). Genetic and chromosome instability. DNA repair. Genetic predisposition syndromes. Cancer cell biology: Control of cell proliferation and cell differentiation: cell cycle; cell signalling. Cancer cell biology: Control of cell homeostasis; control of macromolecular turnover; Autophagy; Control of Cell death (Apoptosis, Necrosis, Autophagic cell death). Molecular Oncology 1: Oncogenes. Main Oncogenes: c-MYC, RAS, others. Oncogene addiction. Molecular Oncology 2: Tumour Suppressors. Main Tumour Suppressor genes: TP53, PTEN, BECLIN1, TSC, others. Tumour cell metabolism: Warburg effect, glutamine, glucose. Oncogenes and oncosuppressors and cancer cell metabolism. Papers’ discussion. Dormancy, Senescence, Telomerases, Cancer Stem Cells. Papers’ discussion. Epigenetics and Cancer. DNMT, HDAC, microRNAs. Oncosuppressor Imprinting (ARH-I/DIRAS3). Papers’ discussion. Tumour stroma. Cancer associated fibroblasts. Cancer inflammation. Papers’ discussion. Cell adhesion. Invasion and Metastasis. Angiogenesis. Tumour immunity. Cachexia. Cancer diagnosis, cancer therapy. Specific cancers: Colon, Breast, Lung, Prostate, Ovary, Melanomas and Skin cancers. Diet and cancer; anticancer properties of phytochemicals, vitamins, natural products. Microbioma and Cancer.
Print
Course Molecular therapy
Course ID MS0465
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader COLANGELO Donato
Teachers Colangelo Donato
CFU 11
SSD BIO/13 - BIOLOGIA APPLICATA, BIO/14 - FARMACOLOGIA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Modules
Course ID Course SSD Teachers Agenda web
MS0466Biotechnological applications BIO/13 - BIOLOGIA APPLICATA Ellis Steven Robert
MS0467Pharmacology BIO/14 - FARMACOLOGIA Colangelo Donato
Show parent course details
Print
Course Molecular therapy: Biotechnological applications
Course ID MS0466
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ELLIS Steven Robert
Teachers Ellis Steven Robert
CFU 6
Teaching duration (hours) 36
Individual study time 114
SSD BIO/13 - BIOLOGIA APPLICATA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type G
Show parent course details
Print
Course Molecular therapy: Pharmacology
Course ID MS0467
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader COLANGELO Donato
Teachers Colangelo Donato
CFU 5
Teaching duration (hours) 25
Individual study time 100
SSD BIO/14 - FARMACOLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Contenuti The course will focus on the analysis of the rational bases for the development of selected classes of target –designed drugs, also considering their pharmacokinetics and pharmacodynamic aspects for a clinical use. Furthermore, the course integrate basic pharmacology with clinic properties. Relevant drugs will be also analyzed in order to point out relevant molecular and diagnostic properties useful for the preclinical development of new biotech drugs.
Testi di riferimento The pharmacological bases of therapy, Goodman e Gilman, XI, Mc Graw-Hill Pharmacology, Rang H.P., Dale M.M., Ritter J.M e Moore P.K., Editor Ambrosiana
Obiettivi formativi The course is intended to provide specific knowledge of pharmacologic constants that rule the therapeutic properties of selected drugs developed with biotech methodologies.
Prerequisiti Basic knowledges of Anatomy, Physiopathology and Internal Medicine
Metodi didattici Frontal Lessons. The teaching activities will take place from the front of the classroom.
Altre informazioni The teacher will meet the students on every Tuesday 2-6 p.m., best after email reservation.
Modalità di verifica dell'apprendimento Multiple choice tests.
Programma esteso The course will focus on the analysis of the rational bases for the development of selected classes of target –designed drugs, also considering their pharmacokinetics and pharmacodynamic aspects for a clinical use. Furthermore, the course integrate basic pharmacology with clinic properties. Relevant drugs will be also analyzed in order to point out relevant molecular and diagnostic properties useful for the preclinical development of new biotech drugs.
Print
Course Molecular virology
Course ID MS0468
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GARIGLIO Marisa
Teachers Gariglio Marisa
CFU 6
Teaching duration (hours) 28
Individual study time 122
SSD MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Testi di riferimento Brooks “Jawetz, Melnick, & Adelberg's Medical Microbiology” - Levinson “Review of Medical Microbiology and Immunology” - Ryan “Sherris Medical Microbiology” - David M Knipe, Peter Howley “Fields Virology” 6th edition
Obiettivi formativi Understanding the interplay between viruses and their host organisms giving to students a view of innate defenses and viral pathogenesis focusing on medically important viruses.
Prerequisiti The students are expected to know basic virology and fundamentals of molecular and cellular biology.
Metodi didattici The course consist of 28h of frontal lessons. All the slides of each lesson will be available to the students (DIR).
Modalità di verifica dell'apprendimento The exam will be divided in two part: written (6 open questions) and oral.
Programma esteso The course deals with basic virology and virological terminology. The effect of virus infection at organism level is addressed. The focus of the course is on molecular virology, especially on virus replication in the infected cell and the molecular mechanisms of this. A selected set of relevant human viruses, differing in their genome structure (RNA versus DNA, circular versus linear) and in their virion architecture will be discussed, the content of the course being specialised with respect to molecular biology, molecular genetics, recombinational events, virus-host interactions and innate immunity. The focus is on medically important viruses, such as tumour viruses, hepatitis viruses, HIV and herpesviruses.
Print
Course Fertility, reproduction and endocrine system biotechnologies
Course ID MS0452
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader MARZULLO Paolo
Teachers Marzullo Paolo
CFU 4
SSD MED/13 - ENDOCRINOLOGIA, MED/40 - GINECOLOGIA E OSTETRICIA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Primo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0453Endocrinology and Metabolism MED/13 - ENDOCRINOLOGIA Marzullo Paolo
MS0454Biotechnology of reproduction MED/40 - GINECOLOGIA E OSTETRICIA Surico Daniela
Show parent course details
Print
Course Fertility, reproduction and endocrine system biotechnologies: Endocrinology and Metabolism
Course ID MS0453
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader MARZULLO Paolo
Teachers Marzullo Paolo
CFU 2
Teaching duration (hours) 10
Individual study time 40
SSD MED/13 - ENDOCRINOLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Show parent course details
Print
Course Fertility, reproduction and endocrine system biotechnologies: Biotechnology of reproduction
Course ID MS0454
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader SURICO Daniela
Teachers Surico Daniela
CFU 2
Teaching duration (hours) 12
Individual study time 38
SSD MED/40 - GINECOLOGIA E OSTETRICIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento english
Metodi didattici power point slides
Modalità di verifica dell'apprendimento The exam consists of a written test containing n° 3 statements. The student must answer true/false and provide a brief explanation
Print
Course Internal Medicine
Course ID MS0459
Academic Year 2017/2018
Year of rule 2017/2018
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CASTELLO Luigi Mario
Teachers Castello Luigi Mario
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/09 - MEDICINA INTERNA
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi The student should demonstrate competence regarding the topics scheduled in the course. Speculative and pragmatic skills will be also tested during the examination in order to assess the application of knowledge in a practical context.
Contenuti The course will transmit skills on the growing role of biotechnology applications both in the diagnostic and in the therapeutic approach to different acute and chronic diseases. The topics that will be object of the lessons have been selected to give examples of diseases characterized by educational relevance in the description of the diagnostic approach strategies (genetic diseases on one hand but also the development of diagnostic strategies in the fight against doping in sport) in the study of pathophysiology (diabetes mellitus, hypercalcemia) and in the therapeutic approach (evolution of the treatment of diabetes mellitus, viral hepatitis and autoimmune diseases such as rheumatoid arthritis). The course will also show some examples of the development of drugs obtained by recombinant DNA techniques and the development of innovative therapeutic strategies such as stem cell transplantation and gene therapy in different conditions.
Testi di riferimento Scientific articles provided by the teacher during the course. The recommended text for consultation is: Harrison's Principles of Internal Medicine, McGraw-Hill Editions, in the latest edition available online for UPO students through Access Medicine.
Obiettivi formativi The aim of the course is to give notions of methodology relating to the main biotechnological applications in Internal Medicine. In particular, the course will transmit skills on the growing role of biotechnology applications both in the diagnostic and in the therapeutic approach to different acute and chronic diseases. The topics that will be object of the lessons have been selected to give examples of diseases characterized by educational relevance in the description of the diagnostic approach strategies (genetic diseases on one hand but also the development of diagnostic strategies in the fight against doping in sport) in the study of pathophysiology (diabetes mellitus, hypercalcemia) and in the therapeutic approach (evolution of the treatment of diabetes mellitus, viral hepatitis and autoimmune diseases such as rheumatoid arthritis). The course will also show some examples of the development of drugs obtained by recombinant DNA techniques and the development of innovative therapeutic strategies such as stem cell transplantation and gene therapy in different conditions.
Prerequisiti The student should possess basic knowledge about the Human Anatomy, Biochemistry, Physiology, Pharmacology and General Pathology.
Metodi didattici Ex-cathedra lectures with supporting slides.
Altre informazioni All the teaching material concerning the course will be available for registered students, on the DIR platform.
Modalità di verifica dell'apprendimento The examination consists in 20 statements to which the students attribute a true or false judgment. Few lines of comment, motivating the answer, are needed. This kind of examination will be performed on the computer, using the DIR platform. If the number of registered students is very low, the teacher can opt for an oral examination.
Programma esteso • Criteria for the definition of sepsis and its study models • Cystic fibrosis: clinical manifestations, diagnosis and principles of gene therapy • Lung tumors: classification and principles of targeted-therapy • The biological basis of autoimmune disease therapy • The biological basis of diabetes mellitus therapy • Physiopathological aspects of paraneoplastic hypercalcemias • Biotechnological strategies in the fight against the use of doping substances in sport • Acute thromboembolic events and platelet activation • Hypertension
Print guide
Print
Year Course ID Course Teachers SSD Curriculum Site CFU
1 MS0452 Fertility, reproduction and endocrine system biotechnologies Marzullo Paolo MED/13, MED/40 All 4
1 MS0455 Functional Genomics Giordano Mara MED/03, BIO/10, BIO/18 All 11
1 MS0459 Internal Medicine Castello Luigi Mario MED/09 All 5
1 MS0465 Molecular therapy Colangelo Donato BIO/13, BIO/14 All 11
1 MS0468 Molecular virology Gariglio Marisa MED/07 All 6
1 MS0469 Neoplastic and proliferative disorders Isidoro Ciro MED/15, MED/04 All 12
1 MS0491 Regenerative Medicine Follenzi Antonia BIO/11, BIO/17 All 10
2 MS0461 Molecular diagnostics Boldorini Renzo Luciano MED/08, BIO/12, MED/05 All 11
2 MS0473 Public health and regulatory aspects Faggiano Fabrizio MED/42, SECS-P/07, MED/01 All 11
2 MS0480 Transplantation and Biocompatibility Rimondini Lia MED/15, MED/14, MED/04, BIO/16, MED/28, BIO/11 All 7
Data synched: 09/07/2019, 13:56