Ateneo - UPO

- Safety in cell culture laboratory
- Instruments and furniture in a cell culture laboratory
- Aseptic techniques
- Cell types and their characteristics
- Cell culture media and supplements
- Cell culture maintenance
- Cell culture protocols: passaging, freezing, thawing
- Cell counts with emocytometer
- Contamination test
- Practical experience in a cell culture laboratory

Teacher slides presented in class

Knowledge of the basic protocols for the correct maintenance of cell lines growing in suspension and in adhesion.

For the access to the practical laboratory it is necessary to pass the planned block exams. Knowledge of basic calculation methods used in chemical / biological laboratories

Lectures in the classroom and practical exercises in the laboratory

- Written test (multiple choices)
- Assay on the practical cell laboratory experience

Frontal lessons on structure and organization of cell culture laboratories, biological risk, sterilization / disinfection methods; Practical lessons in the laboratory with cells growing in adhesion and suspension, freezing and thawing of cells, cell counts, and setting up a growth curve.

At the end of the module, the student knows and knows how to achieve:
- the correct procedures for working in sterile conditions and the main basic techniques for animal cell cultures
- a protocol to evaluate cell growth in different conditions
- can critically evaluate the results obtained through the drafting, under the guidance of the teacher and tutors, of a report concerning the experiments carried out