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Medical Biotechnology

Academic program

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Course Experimental thesis
Course ID MS0451
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
CFU 14
Individual study time 350
SSD PROFIN_S -
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category E
Year 2
Frequenza obbligatoria 1
Grading type G
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Course Internship 2
Course ID MS0496
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
CFU 6
Individual study time 90
SSD NN -
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category F
Year 2
Period Secondo Semestre
Grading type V
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Course Molecular diagnostics
Course ID MS0461
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BOLDORINI Renzo Luciano
Teachers Boldorini Renzo Luciano, Capello Daniela, Rolla Roberta
CFU 11
SSD MED/08 - ANATOMIA PATOLOGICA, BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA, MED/05 - PATOLOGIA CLINICA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 2
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi The student must be able to understand the main carcinogenic and pharmacogenomic mechanisms and their potential applicability in the translational field. It must be able to carry out the main molecular investigations in the oncological field starting from biological materials of different treatment modalities
Testi di riferimento Kumar, Abbas, Fausto. Robbins and Cotran Pathologic Basis of Disease. VII ed Elsevier, 2005-09-07; scientific articles (references given during the course).
Obiettivi formativi The student must be able to understand the translational meaning of molecular diagnostic procedures in the anatomo-pathological field, of clinical biochemistry concerning the main human neoplastic pathologies and the usefulness of pharmacogenomic methods in the clinical context. Main carcinogenic mechanisms and clinical translation of the main oncological biomarkers
Prerequisiti Principles of Pathology, Anatomy and Histology and Molecular Biology.and biochemistry
Metodi didattici Power-point presentations, CD-ROM and educational websites.
Modalità di verifica dell'apprendimento Multiple choice test
Modules
Course ID Course SSD Teachers Agenda web
MS0462Pathologic basis of diseases MED/08 - ANATOMIA PATOLOGICA Boldorini Renzo Luciano
MS0463Clinical applications of Molecular Biology BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA Capello Daniela
MS0464Clinical Pathology MED/05 - PATOLOGIA CLINICA Rolla Roberta
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Course Molecular diagnostics: Pathologic basis of diseases
Course ID MS0462
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BOLDORINI Renzo Luciano
Teachers Boldorini Renzo Luciano
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/08 - ANATOMIA PATOLOGICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Contenuti Diagnostic, prognostic and predictive factors of pathological interest of the main human malignancies: carcinoma of the lung, breast, colorectal, urinary tract, male and female genital, central nervous system, thyroid; major molecular markers of diagnostic, prognostic and predictive interest: technical principles and clinical applications.
Testi di riferimento Kumar, Abbas, Fausto. Robbins and Cotran Pathologic Basis of Disease. VII ed Elsevier, 2005-09-07; scientific articles (references given during the course).
Obiettivi formativi The student must be able to understand the meaning of translational molecular diagnostic procedure in pathological context relating to the main human neoplastic diseases.
Prerequisiti Notions of Pathology, Anatomy and Histology and Molecular Biology.
Metodi didattici Power-point presentations, CD-ROM and educational websites.
Modalità di verifica dell'apprendimento Multiple choice test
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Course Molecular diagnostics: Clinical applications of Molecular Biology
Course ID MS0463
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CAPELLO Daniela
Teachers Capello Daniela
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi At the end of the course students are expected to have acquired a general knowledge about the main molecular strategies utilized for the identification and the analysis of biomarkers in oncology and about the most important molecular targets currently used for cancer diagnosis and therapy.Moreover student will understand the complexity of cancer development and progression and the difficulty, in many neoplastic diseases, of identifying effective therapeutic targets, as the continuous interaction between genotype, phenotype and microenvironment affect and modulate the evolution of neoplasia and its response to therapy
Contenuti OMICS strategies are driving clinical oncology to take a molecular approach to diagnosis, prognostication, and treatment selection. The main objective of the course is providing the basic knowledge for the use of biomarkers in molecular diagnostic and target therapy of cancer
Testi di riferimento Slides; scientific papers discussed during the classes; online public database resources
Obiettivi formativi At the end of the course students will have a general knowledge of 1) the main molecular strategies utilized for the identification and the analysis of biomarkers in oncology: from the genome-wide approaches to the “marker specific”-based methods; 2) the most relevant actionable mutations and biochemical pathways in cancer
Prerequisiti The student are expected to have a general knowledge of molecular biology and genetics and of the main technologies used in genomic investigations
Metodi didattici Slides presented during the course, interactive journal clubs; PDF copy of scientific papers
Altre informazioni All the teaching material and information regarding the course will be available on DIR (https://www.dir.uniupo.it/)
Modalità di verifica dell'apprendimento Evaluation will consist in a written short report on a topic discussed during the course and an oral presentation of a scientific paper with critical discussion. A short, written multiple choice test will allow to evaluate learning concerning the main molecular biology techniques applied to cancer diagnostic.
Programma esteso 1. Molecular strategies utilized for the identification and the analysis of biomarkers in oncology: from the genome-wide approaches to the “marker specific”-based methods2. Mutational landscape of cancer. Definition of drivers and passenger mutations. Classification of tumor driver genes. Strategies for identifying new candidate cancer genes among low frequently mutated genes. The problem of saturation. Mutational heterogeneity in cancer: mutations originated prior tumor initiation and mutations originating during tumor progression. Mutation timer and tumor evolution. Interpatient, intrapatient and intraclonal tumor heterogeneity. Clonal evolution theories. The prospective use of deep sequencing in diagnostic and therapy of cancer.3. Genetic lesions in cancer. Analysis of the most relevant actionable mutations and biochemical pathways in cancer: i) growth factors/tyrosine kinase receptors; ii) PI3K/Akt/mTOR pathway; iii) MAP kinases pathways; iv) cancer stem cells as possible targets in tumor therapy; v) metabolic reprogramming in tumors, hypoxia and neo-angiogenesis; vi) Mechanisms of cancer metastasis: the role of the microenvironment and the role of mutations
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Course Molecular diagnostics: Clinical Pathology
Course ID MS0464
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ROLLA Roberta
Teachers Rolla Roberta
CFU 1
Teaching duration (hours) 4
Individual study time 21
SSD MED/05 - PATOLOGIA CLINICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type G
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Course Optional Activities 2
Course ID MS0498
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
CFU 4
Teaching duration (hours) 40
Individual study time 60
SSD NN -
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category D
Year 2
Period Secondo Semestre
Grading type G
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Course Public health and regulatory aspects
Course ID MS0473
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FAGGIANO Fabrizio
Teachers Faggiano Fabrizio, Gattei Valter, Ferrante Daniela
CFU 11
SSD MED/42 - IGIENE GENERALE E APPLICATA, SECS-P/07 - ECONOMIA AZIENDALE, MED/01 - STATISTICA MEDICA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 2
Period Primo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0474Public Health MED/42 - IGIENE GENERALE E APPLICATA Faggiano Fabrizio
MS0475Innovation and Patents SECS-P/07 - ECONOMIA AZIENDALE Gattei Valter
MS0476Biomedical statistics MED/01 - STATISTICA MEDICA Ferrante Daniela
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Course Public health and regulatory aspects: Public Health
Course ID MS0474
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FAGGIANO Fabrizio
Teachers Faggiano Fabrizio
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD MED/42 - IGIENE GENERALE E APPLICATA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
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Course Public health and regulatory aspects: Innovation and Patents
Course ID MS0475
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GATTEI VALTER
Teachers Gattei Valter
CFU 1
Teaching duration (hours) 8
Individual study time 17
SSD SECS-P/07 - ECONOMIA AZIENDALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
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Course Public health and regulatory aspects: Biomedical statistics
Course ID MS0476
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FERRANTE Daniela
Teachers Ferrante Daniela
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/01 - STATISTICA MEDICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi Knowledge and understanding: to go in depth of statistical methods for the analysis of clinical data. Ability to apply knowledge and understanding: translating theory into practice by concretely working at the solutions to exercises. Decision-making autonomy: acquiring autonomy in decision to conduct the analysis of data obtained from surveys. Communication skills: ability to communicate knowledge with clarity, scientific and methodological rigour. Learning skills: dealing with the information obtained from clinical studies with the objective of collecting and organizing data and correctly interpreting the information.
Contenuti The course consists in a first part with topics related to descriptive statistics and in a second part that will concern the inferential statistics.
Testi di riferimento W.W. Daniel, CL. Cross Biostatistics, Tenth edition. John Wiley & Sons Rosner B. Fundamentals of Biostatistics. Eighth edition. Brooks/Cole Pub Co
Obiettivi formativi The course aims to: - provide methodological issues related to descriptive and inferential statistics - select the appropriate statistical method to summarize study data - interpretation of the results
Prerequisiti Basic knowledge in mathematics
Metodi didattici Lectures, Classroom exercises
Altre informazioni The Course material is available in the Moodle platform.
Modalità di verifica dell'apprendimento Written exam. The exam aims to verify knowledge and skills acquired by the student through the solution of exercises and open questions concerning the course programme
Programma esteso The frequency distribution Measures of Central Tendency and dispersion Properties of Probability Probability distribution: the Poisson and normal distribution Sampling distribution Distribution of the sample mean Confidence interval Hypothesis testing: a single population mean, the difference between two population means, a single population proportion Type I and type II error and the power of a test Chi-square test Odds ratio The correlation coefficient and the regression model Survival analysis
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Course Transplantation and Biocompatibility
Course ID MS0480
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader RIMONDINI Lia
Teachers Gaidano Gianluca, Cantaluppi Vincenzo, Chiocchetti Annalisa, Boccafoschi Francesca, Rimondini Lia, Mantovani Diego
CFU 7
SSD MED/15 - MALATTIE DEL SANGUE, MED/14 - NEFROLOGIA, MED/04 - PATOLOGIA GENERALE, BIO/16 - ANATOMIA UMANA, MED/28 - MALATTIE ODONTOSTOMATOLOGICHE, BIO/11 - BIOLOGIA MOLECOLARE
Course type Attività formativa integrata
Course mandatoriety OBB
Year 2
Period Primo Semestre
Grading type V
Lingua insegnamento The course will be given in English.
Risultati di apprendimento attesi The student is expected to gain knowledge on: a) the main immunological aspects of biocompatibility of transplants and materials including procedures for manufacturing , characterization b) main applications of hematopoietic stem cell transplant, nephrology, dialysis and kidney transplantation, implantable materials for regenerative medicine and drug delivering.
Contenuti The course provides an introduction to immunology and to the immunological responses in various tissues and organs transplantation. Concepts on the procedures of hematopoietic stem cell and kidney transplantation, transplantation are provided as well. The course provides an introduction to biomaterials biocompatibility and implantable applications even when at nanosized.
Testi di riferimento Slides presentations, research and review papers. Text: Edward J. Benz, Helen Heslop, Jeffrey Weitz, John Anastasi, Leslie E. Silberstein, Ronald Hoffman. HEMATOLOGY. BASIC PRINCIPLES AND PRACTICE. Elsevier - Health Sciences Division, 2017. ISBN: 9780323357623
Obiettivi formativi Learning objectives are: a) Knowledge of principle of immunology b) Knowledge on the main immunological reactions in case of organs and tissues tissue transplantation and materials graft c) Knowledge of the hematopoietic stem cell potential; knowledge of the procedures and applications of hematopoietic stem cell transplant; knowledge of GVHD effect and of the therapeutic exploitation of GVT d) Knowledge and understanding of main pathophysiological mechanisms of renal disease and attendant clinical and laboratory manifestations; knowledge and understanding of principles of therapy of renal disorders, with a focus on artificial and natural renal replacement therapy (RRT) and relative biotechnological applications 4) knowledge and understanding of clinical problems of nephropathic patient, including patient with advanced chronic kidney disease (CKD), patient on dialysis and with renal transplant and relative biotechnological applications e) Knowledge of the main requisites for implantable biomaterials and their applications f) development of autonomous judging skills and of adequate communicative skills, (correct medical language) and autonomous learning skills towards main topics of the course.
Prerequisiti Prerequisites of the course are: a) basic knowledge on immunology; b) basic knowledge of chemistry and physics; c) knowledge of the hematopoietic system; basic knowledge of hematopoietic diseases; d) knowledge of flow cytometry; e) basic knowledge of anatomy and renal physiology.
Metodi didattici Classes and self-assessment tests after each class.
Altre informazioni Slides are available on the University DIR website. Other information are available at each professor web-site at upo-book (see www.uniupo.it)
Modalità di verifica dell'apprendimento Written exam, based on three statements concerning the activities performed in class. For each statement, students are invited to assess "true/false" and write a few lines of comment to detail their choice.
Programma esteso a) Concept of immunology, immune-hypersensitivity, histocompatibility and the related diseases, b) The hematopoietic stem cell, hematopoiesis , Regulation of hematopoiesis, The concept of stem cell transplant, types of hematopoietic stem cell transplant (autologous, allogeneic, haploidentical), GVHD and GVT c) Principles of anatomy and renal physiology. Basis of renal semeiotics including instrumental exams to study renal disorders. Renal biopsy. The nephrological syndromes. Primary and secondary glomerulonephritis. Acute kidney injury (AKI). Chronic kidney disease (CKD). Progression of chronic renal failure. Uremic syndrome. Preparation for dialysis. Hemodialysis: general principles and indications. Management of hemodialysis patient. Peritoneal dialysis: general principles and indications. Renal transplant. General principles and indications. Management of renal transplant patient. The immunosuppressive therapy. Complications of renal transplant. The role of C4d in the diagnosis of antibody-mediated rejection. d) Concept of biocompatibility, biomaterials and functionalized biomaterials for tissue substitutions and regeneration, and cancer treatment (including nanomaterials).
Modules
Course ID Course SSD Teachers Agenda web
MS0481Hematopoietic stem cell biology and transplantation MED/15 - MALATTIE DEL SANGUE Gaidano Gianluca
MS0482Biotechnology in kidney transplantation MED/14 - NEFROLOGIA Cantaluppi Vincenzo
MS0483Transplant immunology MED/04 - PATOLOGIA GENERALE Chiocchetti Annalisa
MS0484Materials Biocompatibility BIO/16 - ANATOMIA UMANA Boccafoschi Francesca
MS0485Nanomaterials MED/28 - MALATTIE ODONTOSTOMATOLOGICHE Rimondini Lia
MS0493Molecular strategies for improving materials biocompatibility BIO/11 - BIOLOGIA MOLECOLARE Mantovani Diego
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Course Transplantation and Biocompatibility: Hematopoietic stem cell biology and transplantation
Course ID MS0481
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GAIDANO Gianluca
Teachers Gaidano Gianluca
CFU 1
Teaching duration (hours) 6
Individual study time 19
SSD MED/15 - MALATTIE DEL SANGUE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento The course will be given in English
Risultati di apprendimento attesi At the end of the course, the student will be able to critically analyse the strategies of transplant procedures of hematopoietic stem cells and their application in hematology
Contenuti The course will deal with hematopoietic stem cell transplantation, both autologous and allogeneic, with a particular focus on the aspects that are relevant for Medical Biotechnologists.
Testi di riferimento Testo per la consultazione: Williams Hematology, 9e Author(s): Kenneth Kaushansky, Marshall A. Lichtman, Josef T. Prchal, Marcel M. Levi, Oliver W. Press, Linda J. Burns and Michael Caligiuri (available on UPO off campus library via AccessMedicine)
Obiettivi formativi The aim of the course is to render the student able to know, interpret and analyse the main hematological topics of the course, that are are relevant for a Medical Biotechnologist, with a critical attitude, as requested by the Dublin descriptor 3.
Prerequisiti Knowledge of main blood disorders and of immunology
Metodi didattici A) Taught (frontal) classes with slide presentations available on the UPO DIR website (https://www.dir.uniupo.it/); B) Self-assessment tests after each class
Altre informazioni Participation in the self-assessment activitities after each class is strongly recommended
Modalità di verifica dell'apprendimento The exam will be in the context of the exam of the integrated course. The exam of Hematopoietic Stem Cell Biology and Transplantation will be based on 3 statements, for which the candidate has to: i) assign true/false; ii) write 5-10 lines of comment to justify the choice. Examples of these tests are provided as self-assessment tests after each class.
Programma esteso A. The hematopoietic stem cell B. Hematopoiesis C. General concepts of hematopoietic stem cell transplant D. Autologous stem cell transplant E. Allogeneic and syngeic stem cell transplant F. Graft versus host disease and graft verus tumor effect
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Course Transplantation and Biocompatibility: Biotechnology in kidney transplantation
Course ID MS0482
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CANTALUPPI Vincenzo
Teachers Cantaluppi Vincenzo
CFU 1
Teaching duration (hours) 6
Individual study time 19
SSD MED/14 - NEFROLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi General knowledge of main topics of Nephrology, Dialysis and Transplantation with a focus on biotechnological applications.
Contenuti The course aims at providing general knowledge about management of nephropathic patient, including patient with chronic kidney disease (CKD), on dialysis and with renal transplant, with a focus on modern therapies and diagnostic tools of renal disease and on attendant biotechnological aspects. The following topics will be covered in detail: Assessment of renal function and urinalysis Diagnostic tools in Nephrology including renal biopsy Nephrological syndromes and main renal disorders Chronic kidney disease (CKD) and acute kidney injury (AKI) Artificial renal replacement therapy (RRT): hemodialysis and peritoneal dialysis. Biotechnological applications in dialysis. Naturall renal replacement therapy (RRT): renal transplant. Principles of immunosuppressive therapy. Biotechnological applications in renal transplant.
Testi di riferimento The slides presented during formal lessons represent the reference material for final exam. For further study, main textbooks of Nephrology and selected review articles are available for consultation at the Nephrology and Renal Transplant Unit.
Obiettivi formativi 1) knowledge and understanding of main pathophysiological mechanisms of renal disease; 2) knowledge and understanding of main renal disorders and attendant clinical and laboratory manifestations; 3) knowledge and understanding of principles of therapy of renal disorders, with a focus on artificial and natural renal replacement therapy (RRT) and relative biotechnological applications 4) knowledge and understanding of clinical problems of nephropathic patient, including patient with advanced chronic kidney disease (CKD), patient on dialysis and with renal transplant and relative biotechnological applications 5) development of autonomous judging skills and of adequate communicative skills, (correct medical language) 6) development of autonomous learning skills towards main topics of Nephrology.
Prerequisiti Student must be familiar with basicl principles of anatomy and renal physiology
Metodi didattici The course is based on 3 lessonswhich deal with topics described in the program. Within each lesson attendant biotechnological aspects are highlighted. Students are offered the possibility to explore biotechnological aspects of their interest through direct involvement in laboratory activities. Teaching tools are Videoprojector and PCs with Internet connection, which can be found in the Nephrology and Transplant Unit, where a Nephrological library and main texts and nephrological reviews are also available for further study.
Altre informazioni Prof Marco Quaglia is always available for any further information or request at the Nephrology and Transplant Unit of "Maggiore della Carità" Hospital in Novara (0321-3733786).
Modalità di verifica dell'apprendimento Final exam is based on a written proof which contains "statements". The student is required to judge them (true/false) and to comment on them motivating his judgement. "Statements"are about general topics of Nephrology, Dialysis and Transplantation with biotechnological implications.
Programma esteso Principles of anatomy and renal physiology. Basis of renal semeiotics. Urinalysis and urinary sediment. Study of renal function. Renal ultrasound and other instrumental exams to study renal disorders. Renal biopsy. The nephrological syndromes: isolated urinary abnormalities, nephrotic syndrome, acute nephritic syndrome, rapidly progressive syndrome; acute kidney injury (AKI) and chronic kidney disease (CKD). Primary and secondary glomerulonephritis. Acute and chronic interstitial nephritis. Acuta pyelonephritis. Vascular nephropathies. Acute kidney injury (AKI). Chronic kidney disease (CKD). Progression of chronic renal failure. Uremic syndrome. Preparation for dialysis. Hemodialysis: general principles and indications. Arterial-venous fistula for hemodialysis. Management of hemodialysis patient. Peritoneal dialysis: general principles and indications. Management of hemodialysis patient. Renal transplant. General principles and indications. Management of renal transplant patient. The immunosuppressive therapy. Complications of renal transplant. The role of C4d in the diagnosis of antibody-mediated rejection.
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Course Transplantation and Biocompatibility: Transplant immunology
Course ID MS0483
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CHIOCCHETTI Annalisa
Teachers Chiocchetti Annalisa
CFU 2
Teaching duration (hours) 8
Individual study time 42
SSD MED/04 - PATOLOGIA GENERALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
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Course Transplantation and Biocompatibility: Materials Biocompatibility
Course ID MS0484
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BOCCAFOSCHI FRANCESCA
Teachers Boccafoschi Francesca
CFU 1
Teaching duration (hours) 4
Individual study time 21
SSD BIO/16 - ANATOMIA UMANA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Testi di riferimento Teaching material furnished via DIR
Obiettivi formativi Provide the basis for understanding the biocompatibility of materials for biomedical use
Prerequisiti Cell biology, immunology, molecular biology
Metodi didattici Lectures in class
Modalità di verifica dell'apprendimento Written exam
Programma esteso 1.Biocompatibility: basic concepts 2. Polymers: biomechanical characteristics
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Course Transplantation and Biocompatibility: Nanomaterials
Course ID MS0485
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader RIMONDINI Lia
Teachers Rimondini Lia
CFU 1
Teaching duration (hours) 4
Individual study time 21
SSD MED/28 - MALATTIE ODONTOSTOMATOLOGICHE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type G
Lingua insegnamento English
Risultati di apprendimento attesi Knowledge and understanding of the main properties of matter at nanoscale and the potential application in Medicine. Knowledge of the main methods to manage and characterize matter at the nanoscale
Contenuti See Course program below
Testi di riferimento Papers, reviews, chapters provided during classes
Obiettivi formativi Knowledge and understanding of the main properties of the matter at the nanoscale and their characterization. Knowledge and understanding of the main properties of the nanomaterials used in Medicine and their applications. Development of skills addressed to plan R&D on nanomaterials for Medical applications.
Prerequisiti Fundamentals of chimics and physics
Metodi didattici Lectures
Altre informazioni None
Modalità di verifica dell'apprendimento Written test: Statments assessment and comments
Programma esteso Definition of nanomaterials; properties of the materials at the nanoscale; methods to characterize nanomaterials; methods for nanomaterials manufacturing; esample of nanomaterials used for diagnosis and treatment of various diseases (including cancer); nanocarriers for drugs, nanomaterials for tissue regeneration, toxicology of nanomaterials.
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Course Transplantation and Biocompatibility: Molecular strategies for improving materials biocompatibility
Course ID MS0493
Academic Year 2019/2020
Year of rule 2018/2019
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader MANTOVANI DIEGO
Teachers Mantovani Diego
CFU 1
Teaching duration (hours) 8
Individual study time 17
SSD BIO/11 - BIOLOGIA MOLECOLARE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Primo Semestre
Grading type G
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Course Molecular virology
Course ID MS0468
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GARIGLIO Marisa
Teachers Gariglio Marisa
CFU 6
Teaching duration (hours) 28
Individual study time 122
SSD MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Focusing on the most medically important viruses, including Papillomavirus, Polyomavirus, HIV, Hepacivirus and Cytomegalovirus, on completion of the course the student shall be able to: - account for basic knowledge about viral structure, classification, cultivation, replication strategies, interactions with host cell, and methods of detection - account for the interplay between viruses and their host organisms focusing on innate defenses and viral pathogenesis
Contenuti The course deals with basic virology and virological terminology. The effect of virus infection at organism level is addressed. The focus of the course is on molecular virology, especially on virus replication in the infected cell and the molecular mechanisms of this. A selected set of relevant human viruses, differing in their genome structure (RNA versus DNA, circular versus linear) and in their virion architecture will be discussed, the content of the course being specialised with respect to molecular biology, molecular genetics, recombinational events, virus-host interactions and innate immunity. The focus is on medically important viruses, such as tumour viruses, hepatitis viruses, HIV and herpesviruses.
Testi di riferimento The recommended textbooks are: Brooks “Jawetz, Melnick, & Adelberg's Medical Microbiology” Levinson “Review of Medical Microbiology and Immunology” Ryan “Sherris Medical Microbiology” ON ARRIVAL: David M Knipe,Peter Howley“Fields Virology” 6th edition Additional material can be found at: http://www.virology.ws/course/
Obiettivi formativi Understanding the interplay between viruses and their host organisms giving to students a view of innate defenses and viral pathogenesis focusing on medically important viruses.
Prerequisiti The students are expected to know basic virology and fundamentals of molecular and cellular biology.
Metodi didattici All the slides of each lesson will be available to the students.
Modalità di verifica dell'apprendimento 6 questions with true or false answer and explanation An oral exam will be following in order to discuss and complete (if necessary) the written exam.
Programma esteso The course deals with basic virology and virological terminology. The effect of virus infection at organism level is addressed. The focus of the course is on molecular virology, especially on virus replication in the infected cell and the molecular mechanisms of this. A selected set of relevant human viruses, differing in their genome structure (RNA versus DNA, circular versus linear) and in their virion architecture will be discussed, the content of the course being specialised with respect to molecular biology, molecular genetics, recombinational events, virus-host interactions and innate immunity. The focus is on medically important viruses, such as tumour viruses, hepatitis viruses, HIV and herpesviruses.
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Course Internship 1
Course ID MS0495
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
CFU 4
Individual study time 60
SSD NN -
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category F
Year 1
Period Secondo Semestre
Grading type V
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Course Regenerative Medicine
Course ID MS0491
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FOLLENZI Antonia
Teachers Pelicci Giuliana, Follenzi Antonia
CFU 10
SSD BIO/11 - BIOLOGIA MOLECOLARE, BIO/17 - ISTOLOGIA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Secondo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0492Molecular Biology BIO/11 - BIOLOGIA MOLECOLARE Pelicci Giuliana
MS0479Histology, Gene and Cell Therapy BIO/17 - ISTOLOGIA Follenzi Antonia
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Course Regenerative Medicine: Molecular Biology
Course ID MS0492
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader PELICCI Giuliana
Teachers Pelicci Giuliana
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD BIO/11 - BIOLOGIA MOLECOLARE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Provide basic knowledge of stem cell biology, reprogramming and pluripotency with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.
Contenuti Review of general topics of Molecular Biology relevant to the understanding of the course. Chromatin Remodeling: Histone Modifications, DNA methylation Structural classes of transcription factors (Myc, Fos) Epigenetic definition: epigenetic diseases and epigenetic therapies. Oncogenes and tumor suppressor genes. P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function. Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions. Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells. Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep). Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency. Parallelisms between epigenetic reprogramming and cancer. Therapeutic use of iPS Cells and embryonic stem cells.
Testi di riferimento Molecular Biology of the Cell Alberts Scientific Papers
Obiettivi formativi Provide basic knowledge of stem cell biology, reprogramming and pluripotency with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.
Prerequisiti A basic understanding of cell biology and molecular biology.
Metodi didattici PowerPoint presentations of lessons and articles provided by the lecturer
Modalità di verifica dell'apprendimento Written Examination and oral presentation of a scientific journal article.
Programma esteso Review of general topics of Molecular Biology relevant to the understanding of the course. Chromatin Remodeling: Histone Modifications, DNA methylation Structural classes of transcription factors (Myc, Fos) Epigenetic definition: epigenetic diseases and epigenetic therapies. Oncogenes and tumor suppressor genes. P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function. Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions. Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells. Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep). Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency. Parallelisms between epigenetic reprogramming and cancer. Therapeutic use of iPS Cells and embryonic stem cells.
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Course Regenerative Medicine: Histology, Gene and Cell Therapy
Course ID MS0479
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FOLLENZI Antonia
Teachers Follenzi Antonia
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD BIO/17 - ISTOLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Testi di riferimento For italians: Lollini, Terapia genica, ZANICHELLI Animated gene therapy course, Utah University, USA Journals: Human Gene Therapy Gene Therapy Cancer Gene Therapy Journal of Gene Medicine Molecular Therapy Nature Science Databases: NCBI - MEDLINE, GenBank, Genomi, ecc. Genetic Modification Clinical Research Information System (GeMCRIS) Clinicaltrials USA Wiley clinical trial database
Obiettivi formativi The students should be come able to discuss cell and gene therapy published articles and fundamental vectorology used in gene therapy in preclinical studies and clinical trials.
Metodi didattici Slides and PDF articles
Altre informazioni Group seminar prepared by the students on a chosen topic.
Modalità di verifica dell'apprendimento Written test including a commentary on a cell and gene therapy paper and an oral discussion on a topic of the course program.
Programma esteso Stem cells, cellular reprogramming and use of these cells in cell therapy protocols in preclinical and potential applications in the clinic. Isolation, in vitro expansion and preparation of cells for experimental cell therapy. Vectors used for gene transfer: viral vectors and non-viral. Origin, construction and modification. Modulation of Expression cassettes: Constitutive and regulated expression. transcriptional or post-transcriptional expression regulation Immune response to vectors and transgenes used in gene transfer. Concepts of ex vivo and in vivo gene therapy. Clinical trials using cell and gene therapy strategies of target diseases.
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Course Optional Activities 1
Course ID MS0497
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
CFU 4
Teaching duration (hours) 40
Individual study time 60
SSD NN -
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category D
Year 1
Period Secondo Semestre
Grading type G
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Course Molecular therapy
Course ID MS0465
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader COLANGELO Donato
Teachers Ellis Steven Robert, Colangelo Donato
CFU 11
SSD BIO/13 - BIOLOGIA APPLICATA, BIO/14 - FARMACOLOGIA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi The students are expected to acquire the knowledge of the main topics of Pharmacology and Biotechnological applications and to approach with criticism the scientific problems of these topics, together with the ability to set up experimental designs in vitro and in vivo.
Contenuti The course integrates Pharmacology and Biotechnological applications. It will focus mainly on the rational bases for the development of selected classes of target –designed drugs. The course will also analyze relevant molecular and diagnostic properties useful for the preclinical development of new biotech drugs.
Testi di riferimento The learning material and specific texts will be discussed together with the students. The professors will furnish to the students some didactic material as pdf and ppt format before the lessons. Pleas consult the specific D.I.R website of Prof. Colangelo and Prof. Ellis. Access keywords will be given to the students during the first lesson.
Obiettivi formativi The course is intended to provide specific knowledge of pharmacology and biotechnological applications of selected drugs developed with biotech methodologies.
Prerequisiti Basic knowledges of Immunology, Anatomy, Physiopathology and Pathology.
Metodi didattici Frontal Lessons. All teaching activities will take place in front of the classroom. The learning material will be furnished to the students before the lessons as pdf. It can be found on the specific D.I.R website of Prof. Colangelo and Prof. Ellis with a keyword that will be given to the students during the first lesson.
Altre informazioni The teachers will meet the students on specific days. Please refer to each specific course description. We suggest preventive email reservation.
Modalità di verifica dell'apprendimento Multiple choice tests. For the assessment of the student preparation, a numerical grading will be adopted. It that will range between 18 (minimum) and 30 with laude (maximum), according to the preparation of the student. In detail: From 18 to 21: the student has a limited preparation and a limited ability to allocate the information learned in the course in a scientific landscape. From 22 to 24: the student has a sufficient but partial preparation and a limited ability to allocate the information learned in the course in a scientific landscape. From 25 to 27: the student has a good preparation and sufficient ability to allocate the information learned in the course in a scientific landscape. From 28 to 30: the student has an optimal preparation and a good ability to allocate the information learned in the course in a scientific landscape. 30 laude: the student has an excellent preparation.
Programma esteso The course will focus on the analysis of the rational bases for the development of selected classes of target –designed drugs for clinical use. Furthermore, the course integrates basic pharmacology and biotech applications for preclinical development of new biotech drugs.
Modules
Course ID Course SSD Teachers Agenda web
MS0466Biotechnological applications BIO/13 - BIOLOGIA APPLICATA Ellis Steven Robert
MS0467Pharmacology BIO/14 - FARMACOLOGIA Colangelo Donato
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Course Molecular therapy: Biotechnological applications
Course ID MS0466
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ELLIS Steven Robert
Teachers Ellis Steven Robert
CFU 6
Teaching duration (hours) 36
Individual study time 114
SSD BIO/13 - BIOLOGIA APPLICATA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
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Course Molecular therapy: Pharmacology
Course ID MS0467
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader COLANGELO Donato
Teachers Colangelo Donato
CFU 5
Teaching duration (hours) 25
Individual study time 100
SSD BIO/14 - FARMACOLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Expected learning outcomes: The students are expected to acquire the knowledge of the main topics of Pharmacology and to approach with criticism the scientific problems of this topic, together with the ability to set up an experimental design related to Pharmacology in vitro and in vivo.
Contenuti Course contents: The course will focus on the analysis of the rational bases for the development of selected classes of target –designed drugs, also considering their pharmacokinetics and pharmacodynamic aspects for a clinical use. Furthermore, the course integrates basic pharmacology with clinic properties. Relevant drugs will be also analyzed in order to point out relevant molecular and diagnostic properties useful for the preclinical development of new biotech drugs.
Testi di riferimento Reference texts: The pharmacological bases of therapy, Goodman e Gilman, XI, Mc Graw-Hill Pharmacology, Rang H.P., Dale M.M., Ritter J.M e Moore P.K., Editor Ambrosiana
Obiettivi formativi Course aims: The course is intended to provide specific knowledge of pharmacologic constants that rule the therapeutic properties of selected drugs developed with biotech methodologies. Furthermore, the course intends to provide the bases for the setting of in vivo and in vitro studies conduction and for the interpretation of the results of new molecules with therapeutic potential.
Prerequisiti Student backgroud: Basic knowledges of Anatomy, Physiopathology and Pathology.
Metodi didattici Provision of the course: Frontal Lessons. All teaching activities will take place in front of the classroom. The learnig material will be furnished to the students before the lessons as pdf. It can be found on the specific D.I.R website with a keyword that will be given during the first lesson.
Altre informazioni Office hours: The teacher will meet the students on every Tuesday 2-6 p.m., best after email reservation.
Modalità di verifica dell'apprendimento Verification of learning: Multiple choice tests. For the assessment of the student preparation, a numerical grading will be adopted. It that will range between 18 (minimum) and 30 with laude (maximum), according to the preparation of the student. In detail: From 18 to 21: the student has a limited preparation and a limited ability to allocate the information learned in the course in a scientific landscape. From 22 to 24: the student has a sufficient but partial preparation and a limited ability to allocate the information learned in the course in a scientific landscape. From 25 to 27: the student has a good preparation and sufficient ability to allocate the information learned in the course in a scientific landscape. From 28 to 30: the student has an optimal preparation and a good ability to allocate the information learned in the course in a scientific landscape. 30 laude: the student has an excellent preparation.
Programma esteso Extended program: The course will focus on the analysis of the rational bases for the development of selected classes of target –designed drugs, also considering their pharmacokinetics and pharmacodynamic aspects for a clinical use. Furthermore, the course integrate basic pharmacology with clinic properties. Relevant drugs will be also analyzed in order to point out relevant molecular and diagnostic properties useful for the preclinical development of new biotech drugs.
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Course Functional Genomics
Course ID MS0455
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GIORDANO Mara
Teachers Giordano Mara, Baldanzi Gianluca, Gagliardi Mara, Zucchelli Silvia
CFU 11
SSD MED/03 - GENETICA MEDICA, BIO/10 - BIOCHIMICA, BIO/18 - GENETICA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Primo Semestre
Grading type V
Lingua insegnamento ENGLISH
Modules
Course ID Course SSD Teachers Agenda web
MS0456Genomic analysis MED/03 - GENETICA MEDICA Giordano Mara
MS0457Regulation of gene expression BIO/10 - BIOCHIMICA Baldanzi Gianluca
MS0458Genetics BIO/18 - GENETICA Gagliardi Mara, Zucchelli Silvia
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Course Functional Genomics: Genomic analysis
Course ID MS0456
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GIORDANO Mara
Teachers Giordano Mara
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD MED/03 - GENETICA MEDICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Extensive knowledge of the Human genome and of new generation genomic analysis methods with particular attention to the identification of genes involved in genetic diseases.
Contenuti Human Genome Project. Structure and Evolution of the Human Genome. Methods of studying monogenic and multifactorial diseases. Analysis of genomic rearrangements by CGH array. Next Generation Sequencing Techniques (NGS). Genomic imprintig. Methods for studying epigenetic variations in the Human genome.
Testi di riferimento Articles in Juornals chosen by the Professor
Obiettivi formativi The course aims to illustrate the main approaches of genomic analysis. The first part of the course will be used to understand the structure and evolution of the genome that are the main requirements and then move on to genomic analysis methods. Students will then learn to apply DNA-based microarrays and next-generation sequencing technologies, addressing their problems and understanding their perspectives.
Prerequisiti The student is supposed to know the basic genetics and the modalities of transmission of Mendelian diseases. Moreovere the students must know the main molecular genetic techniques.
Metodi didattici Frontal lessons with slides. Student seminars as an in-depth study of topics covered in class. Tutorials through interactive quizzes. Exercises in the computer classroom
Modalità di verifica dell'apprendimento The final exam for the comprehensive course will consist in a written exam including all the three subjects of the course, namely Genomic Analysis, Regulation of gene expression and Genetics. For each subjects there will be four statements requiring the answer true/false and eventually a brief comment of maximum 10 lines. The maximum time allowed will be 90 minutes. The results will be given within three-four days and if the student wishes to increase the grade he/she can do an oral exam.
Programma esteso Human Genome Project: the main stages of the project and the methodology Structure and Evolution of the Human Genome. Chromatin and chromosome structure. Transposable elements in the genome. Transposons function during evolution. Repeated sequences in the genome. Function of Copy Number Variations. Genome analysis. Methods of studying monogenic and multifactorial diseases. Analysis of genomic rearrangements by CGH array. New Generation Sequencing Techniques (NGS). Genome sequencing. Exome sequencing. Sequencing of the transcriptome. Genomic imprintig. Methods for studying epigenetic variations in the Human genome. Analysis of genomic methylation. Identification of genes that cause genetic diseases in humans through NGS. Study of multifactorial diseases by GWAS (Genome wide association study).
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Course Functional Genomics: Regulation of gene expression
Course ID MS0457
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BALDANZI GIANLUCA
Teachers Baldanzi Gianluca
CFU 5
Teaching duration (hours) 20
Individual study time 95
SSD BIO/10 - BIOCHIMICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento ENGLISH
Risultati di apprendimento attesi Understanding the mechanisms of regulation of gene expression and how such knowledge can be used in research or therapy.
Contenuti After a quick review of gene structure in eukaryotes and prokaryotes, the issue of gene expression regulation will be presented. The first part of the course will deal with prokaryotes gene expression and its regulation. A series of examples will illustrate the possibility of engineering promoters and genes for biotechnological purposes. The second part of the course will deal with the eukaryotes gene expression and the multiple levels of its regulation. Issues: 1) chromatin organization and structure, 2) transcription factor function, 3) pre-initiation complex, 4) transcription termination, 5) RNA stability and 6) RNA translation. The third part of the course will deal with the mechanisms by which extracellular signals and intracellular cues regulates gene expression in eukaryotes. A particular emphasis will be placed on the alterations of gene expression in cancer and on the regulation of gene expression in function of nutrients and metabolic status of the cell. Basic biochemical techiques
Testi di riferimento For the improvement of students background Harper's Illustrated Biochemistry, 29e, Robert K. Murray, David A. Bender, Kathleen M. Botham, Peter J. Kennelly, Victor W. Rodwell, P. Anthony Weil Please note: available on Access Medicine for our students Molecular Biology of the Cell - Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter Edition:5th Please note: the 4th edition is available for free on NCBI bookshelf http://www.ncbi.nlm.nih.gov/books/NBK21054/ Suggested textbook Genomes 3 By Terence A. Brown Please note: the 2nd edition is available for free on NCBI bookshelf http://www.ncbi.nlm.nih.gov/books/NBK21128/ Cell Signalling Biology Professor Sir Michael Berridge http://www.biochemj.org/csb/ An extended series of reviews evidenced during the course.
Obiettivi formativi Strengthen the knowledge and operational skills in molecular biology applied to the study of gene expression. Provide the operational bases in the biochemical laboratory.
Prerequisiti Basic Biology: Prokaryotes and Eukaryotes intracellular organization. Basic biochemistry: DNA and RNA structure and metabolism, protein synthesis, enzyme proprieties, major metabolic pathways. Basic molecular biology: concept of gene, gene expression, promoter, transcription factor, RNA synthesis and degradation. For the students lacking a strong background in life sciences a series of readings will be proposed to improve their skills on those topics.
Metodi didattici During the course, slides and webinars will be extensively used. Classwork will be complemented by an introduction in the laboratory. Students will also undertake teacher driven research/presentation/teamwork. A copy of the slides and the websites used during the course will be available on the DIR site.
Altre informazioni -
Modalità di verifica dell'apprendimento The final exam for the comprehensive course will consist in a written exam including all the three subjects of the course, namely Genomic Analysis, Regulation of gene expression and Genetics. For each subjects there will be four statements requiring the answer true/false and eventually a brief comment of maximum 10 lines. The maximum time allowed will be 90 minutes. The results will be given within three-four days and if the student wishes to increase the grade he/she can do an oral exam.
Programma esteso Topic 1: Gene expression regulation Recall of the organization of genes in eukaryotes (enhancers, promoters, transcription start site, introns/exons, transcription termination sites). Control of gene transcription at the level of transcription initiation rate, splicing and alternative splicing, mRNA stability, RNA interference and microRNA. Transcription factors organization: structure of DNA binding and trans-activating domains. Major DNA regulatory sequences. Assembly of RNApolII transcription initiation complex. Methods to study DNA regulatory elements, Promoter structure and transcription factor binding sites. Transcription factors and chromatin structure, chromatin remodeling and gene expression. Post transcriptional modification of histones and the histone code. DNA mutilation. Other ways to regulate gene expression: alternative promoters, alternative splicing, control of mRNA stability, control of mRNA translation, miRNA and RNA interference. Topic 2: Control of gene expression by extracellular and intracellular cues. How signal transduction pathways regulates gene expression: The nuclear receptor family The STAT family ERK signaling to the serum response elements. Myc in growth factors signaling and tumor formation Hif in the response to hypoxia and tumorigenesis P53 mutations, cancer and metabolism Topic 3: Tumor cell metabolism and the way to study it. Peculiarities of tumor metabolism, the Warburg effect and beyond. Methods to study metabolism, integrating gene expression, metabolomics and in vivo data. Use of mass spectrometry, PET/NMR probes, and electrodes (Oxygen Consumption Rate – ExtraCellular Acidification Rate) to study metabolism in living cells/tissues/patients. Experimental manipulation of metabolism and possible routes for cancer therapy. Topic 4: How oncogenes and altered signaling pathways regulates tumor cell metabolism. Tumor metabolomics, onco-metabolites and the peculiarities of tumor metabolism. How alterations of signal transduction machinery and gene expression integrates with a peculiar micro-environment to dictate tumor metabolism. How alterations of the metabolism contributes to tumor development (proliferation, migration, resistance to cell death).
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Course Functional Genomics: Genetics
Course ID MS0458
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ZUCCHELLI Silvia
Teachers Gagliardi Mara, Zucchelli Silvia
CFU 1
Teaching duration (hours) 8
Individual study time 17
SSD BIO/18 - GENETICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento ENGLISH
Risultati di apprendimento attesi D1. KNOWLEDGE AND UNDERSTANDING. At the end of the course, the student must demonstrate adequate knowledge of: the complexity of mammalian genomes and the role of the non-coding portion of the genome in driving organismal complexity; the functional use of genome complexity and pervasive transcription; the non-coding RNA revolution; main biological features of lncRNAs as compared to protein-coding mRNAs and lncRNA classification; modes of action of lncRNAs; specific examples of lncRNAs; applicability of lncRNAs in therapy. D2. APPLYING KNOWLEDGE AND UNDERSTANDING. The student will have to show the ability to use acquired knowledge and concepts to reason critically, showing the ability to make connections between different topics and to apply the acquired knowledge to analyze and propose biology experiments. D3. MAKING JUDGEMENT. The students must demonstrate their ability to critically examine the information acquired and to independently discuss problems and applications related to lncRNAs and their role in regulation of gene expression. D4. COMMUNICATION SKILLS. Using open-questions in the written text and the option of an oral exam, the students must demonstrate their ability to discuss the subjects of the course in a clear and comprehensible way and using the appropriate scientific terminology. D5. LEARNING SKILLS. Using a published-papers teaching approach, the students are exposed to the most recent developments and knowledge in the field of functional genomics of lncRNAs. Students therefore will deepen their critical and scientific learning attitude, required for their entry in the laboratory for their master thesis project.
Contenuti The course “Functional Genomics – Genetics” provides knowledge about the complexity of the mammalian genome, the description of the non-coding part of the genome and the definition of long non-coding RNAs and how they contribute to regulation of gene expression.
Testi di riferimento No textbook. Students will study on the scientific papers discussed during the classes and made available from the professor.
Obiettivi formativi The aim of this course is to learn most recent data on non-coding elements in the genome and in mammalian transcriptome. Specifically, the course will focus on long non-coding RNAs (lncRNAs) their structure and functions. Moreover, the students will know about antisense transcription, its main features and its function in the cell. Examples will be provided on possible therapeutic applications of lncRNAs. An additional aim of the course is the discussion of a recent published paper focusing on recently discovered lncRNA and its function. Experimental details will be discussed.
Prerequisiti The students must have a solid background in Molecular Biology, Genetics and Cell Biology
Metodi didattici The students receive copy of PDF files of scientific papers discussed in the course.
Altre informazioni nputs from the professor on bibliographic search in the field of lncRNAs.
Modalità di verifica dell'apprendimento The final exam for the comprehensive course will consist in a written exam including all the three subjects of the course, namely Genomic Analysis, Regulation of gene expression and Genetics. For each subjects there will be four statements requiring the answer true/false and eventually a brief comment of maximum 10 lines. The maximum time allowed will be 90 minutes. The results will be given within three-four days and if the student wishes to increase the grade he/she can do an oral exam.
Programma esteso Complexity of the human genome and role of non-coding elements in contributing to genome complexity Definition of mammalian transcriptome and history of the discovery of lncRNAs Features of lncRNAs in comparison with those of protein-coding mRNAs Description of the experimental procedures used to discover lncRNA features Antisense transcription Description of lncRNA functions Therapeutic applications of lncRNAs: present and future prospects Seminar-type discussion on a newly discovered lncRNA: features and function (experimental details will also be discussed)
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Course Fertility, reproduction and endocrine system biotechnologies
Course ID MS0452
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader MARZULLO Paolo
Teachers Marzullo Paolo, Surico Daniela
CFU 4
SSD MED/13 - ENDOCRINOLOGIA, MED/40 - GINECOLOGIA E OSTETRICIA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Primo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0453Endocrinology and Metabolism MED/13 - ENDOCRINOLOGIA Marzullo Paolo
MS0454Biotechnology of reproduction MED/40 - GINECOLOGIA E OSTETRICIA Surico Daniela
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Course Fertility, reproduction and endocrine system biotechnologies: Endocrinology and Metabolism
Course ID MS0453
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader MARZULLO Paolo
Teachers Marzullo Paolo
CFU 2
Teaching duration (hours) 10
Individual study time 40
SSD MED/13 - ENDOCRINOLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi - To demonstrate the knowledge of the main endocrine and / or metabolic pathologies, the mechanisms of onset and genetic transmission, the relationship between pathophysiology and biotechnologies underlying the treatment. - To define the implication in the field of medical biotechnology. - To use learning for analysis and discussion in simulated contexts.
Contenuti Teaching the pathophysiology of the endocrine system and of the main endocrine-metabolic diseases, focusing the content to the target of the medical biotechnologies. The content of the lessons privileges the relationship between the molecular mechanisms and the etiopathogenetic / therapeutic aspects of endocrine and metabolic disorders. The contents of the course are divided as follows: - General pathophysiology of the endocrine system - Neuroendocrine tumors including hypothalamic-pituitary diseases - Benign and malignant thyroid and parathyroid diseases - Adrenal diseases - Obesity and diabetes mellitus
Testi di riferimento Eric I. Felner, Guillermo E. Umpierrez. Endocrine pathophysiology. Lippincott Williams And Wilkins (12/2013)
Obiettivi formativi Aims of the Course are to provide the Student with broad knowledge on the theoretical, technical and clinically-oriented aspects of the endocrine system and main endocrinopathies, to enhance awareness of the basic sciences and research techniques underpinning endocrinology and metabolism, as well as to transfer information on latest Literature evidence and critical appraisal of novel biotechniques applicable to this discipline.
Prerequisiti Fundamental knowledge in the areas of Anatomy, Physiology and General Pathology preliminary to learning Endocrinology.
Metodi didattici Power-point presentations. Scientific articles from peer-reviewed journals.
Modalità di verifica dell'apprendimento The final exam for the course of Fertility, Reproduction and Endocrine System Biotechnologies will be done in a single session according to the prescheduled yearly timetable. It will consist of a written exam, and will include two consecutive tests, one for each subject of the course with lecturers present during the tests. Each test will consist of three statements queries, each statement will require a 5-to-10 lines answer. The maximum time allowed for each test will be 30 mins (one hour total). Test results will be available the day after the exam.
Programma esteso The endocrine system. The importance of biotechnologies for classification, characterization, understanding and treatment of endocrine diseases. Pituitary axes, hypothalamo-pituitary neoplasms, genetic disorders. Pharmaceutical targeting, combined approaches. Neuroendocrine tumors. Pharmaceutical approach. Molecular therapies. Thyroid dysfunctions, sporadic and familial diseases, thyroid nodules and tumors. Adrenal physiology, glucocorticoid and mineralcorticoid-related diseases, sporadic and genetic disorders of the adrenal medulla. Pathophysiology and causes of obesity and diabetes mellitus, endocrine disrupting chemicals, neuroendocrine control of energy expenditure and appetite, metabolic syndrome, adipocytes, central and peripheral pharmaceutical targets. Gonadal dysfunctions, hypogonadisms, policystic ovary syndrome
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Course Fertility, reproduction and endocrine system biotechnologies: Biotechnology of reproduction
Course ID MS0454
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader SURICO Daniela
Teachers Surico Daniela
CFU 2
Teaching duration (hours) 12
Individual study time 38
SSD MED/40 - GINECOLOGIA E OSTETRICIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento english
Risultati di apprendimento attesi Be familiar with causes of male and female infertility, ICSI/FIVET procedure, cryopreservation and pre-implant diagnosis techniques actually disposable.
Contenuti Female and male infertility and diagnostic program for infertile couple. Assisted fertilization technologies ( IVF and ICSI procedure)Basic aspects of human implantation. Future strategies to improve implantation in patients undergoing ART Variables involved in the outcome of cryopreservation.
Testi di riferimento Human fertility: methods and protocols. Editors: Rosenwaks, Zev, Wassarman, Paul M., 2014ISBN 978-1-4939-0659-8
Obiettivi formativi At the end of the course, the student should be able to describe causes of male and famale infertility, ICSI/FIVET procedure, cryopreservation and pre-implant diagnosis techniques actually disposable.
Prerequisiti Basic knowledge of pelvica male and female anatomy and ovarian cycle
Metodi didattici power point presentation
Altre informazioni -
Modalità di verifica dell'apprendimento The exam is inserted in a test that includes all the activities partecipating at the integrated course. Evaluation of the student consists in a written test formed by 6 questions. The student has to answer true or false giving a brief explanation.The student will have 60 minutes to complete the esam.
Programma esteso Female and male infertility: causes and risk factors. Physiology of aging ovary and checking for ovarian reserve Diagnostic program for infertile couple Development of assisted fertilization technologies (IVF in animals, IVF in human ICSI in animals, ICSI in human) In vitro production of functional gametes Basic physiology of oogenesis Ovarian stimulation. IVF and ICSI procedureBasic aspects of human implantation. Current strategies to improve implantation in patients undergoing ART .Improving endometrial receptivity in IVF patients. Selecting chromosomally normal embryos in patients affected by recurrent miscarriages. Future strategies to improve implantation in patients undergoing ART Variables involved in the outcome of cryopreservation. Clinical results, survival, fertilization of cryopreserved oocytes, embryos and sperm
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Course Internal Medicine
Course ID MS0459
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CASTELLO Luigi Mario
Teachers Castello Luigi Mario, Bellan Mattia
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/09 - MEDICINA INTERNA
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi The student should demonstrate competence regarding the topics scheduled in the course. Speculative and pragmatic skills will be also tested during the examination in order to assess the application of knowledge in a practical context.
Contenuti The course will transmit skills on the growing role of biotechnology applications both in the diagnostic and in the therapeutic approach to different acute and chronic diseases. The topics that will be object of the lessons have been selected to give examples of diseases characterized by educational relevance in the description of the diagnostic approach strategies (genetic diseases on one hand but also the development of diagnostic strategies in the fight against doping in sport) in the study of pathophysiology (diabetes mellitus, hypercalcemia) and in the therapeutic approach (evolution of the treatment of diabetes mellitus, viral hepatitis and autoimmune diseases such as rheumatoid arthritis). The course will also show some examples of the development of drugs obtained by recombinant DNA techniques and the development of innovative therapeutic strategies such as stem cell transplantation and gene therapy in different conditions.
Testi di riferimento For consultation: Harrison’s Principles of Internal Medicine - 17th edition - New York: McGraw-Hill, 2009. References on specific topics will be provided during the course.
Obiettivi formativi The aim of the course is to give notions of methodology relating to the main biotechnological applications in Internal Medicine. In particular, the course will transmit skills on the growing role of biotechnology applications both in the diagnostic and in the therapeutic approach to different acute and chronic diseases. The topics that will be object of the lessons have been selected to give examples of diseases characterized by educational relevance in the description of the diagnostic approach strategies (genetic diseases on one hand but also the development of diagnostic strategies in the fight against doping in sport) in the study of pathophysiology (diabetes mellitus, hypercalcemia) and in the therapeutic approach (evolution of the treatment of diabetes mellitus, viral hepatitis and autoimmune diseases such as rheumatoid arthritis). The course will also show some examples of the development of drugs obtained by recombinant DNA techniques and the development of innovative therapeutic strategies such as stem cell transplantation and gene therapy in different conditions.
Prerequisiti The student should possess basic knowledge about the Human Anatomy, Biochemistry, Physiology, Pharmacology and General Pathology.
Metodi didattici Lectures with supporting slides
Altre informazioni The student may request to attend the laboratory of Emergency Medicine, with targets to be agreed with the teacher.
Modalità di verifica dell'apprendimento The final test will consist of multiple choice quiz or oral exam at the discretion of the teacher based on the number of candidates.
Programma esteso Cystic fibrosis: clinical diagnosis and principles of gene therapy The lung cancer (overview of gene therapy and targeted-therapy) Biotechnological aspects in the study of neuro-degenerative diseases Biotechnological aspects in the study of neuro-inflammatory diseases Aspects of the biotechnological treatment of viral hepatitis The biological basis of treatment of autoimmune diseases The biological basis of treatment of diabetes mellitus Pathophysiological aspects of paraneoplastic hypercalcemia Strategies for the use of biotechnology against doping in sport Use of new biomarkers in emergency medicine
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Course Neoplastic and proliferative disorders
Course ID MS0469
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ISIDORO Ciro
Teachers Gaidano Gianluca, Isidoro Ciro
CFU 12
SSD MED/15 - MALATTIE DEL SANGUE, MED/04 - PATOLOGIA GENERALE
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Primo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi refer to MS0470 and MS0471
Contenuti refer to MS0470 and MS0471
Testi di riferimento refer to MS0470 and MS0471
Obiettivi formativi refer to MS0470 and MS0471
Prerequisiti refer to MS0470 and MS0471
Metodi didattici refer to MS0470 and MS0471
Altre informazioni refer to MS0470 and MS0471
Modalità di verifica dell'apprendimento refer to MS0470 and MS0471
Programma esteso refer to MS0470 and MS0471
Modules
Course ID Course SSD Teachers Agenda web
MS0470Hematoncology MED/15 - MALATTIE DEL SANGUE Gaidano Gianluca
MS0471Experimental oncology MED/04 - PATOLOGIA GENERALE Isidoro Ciro
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Course Neoplastic and proliferative disorders: Hematoncology
Course ID MS0470
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GAIDANO Gianluca
Teachers Gaidano Gianluca
CFU 5
Teaching duration (hours) 40
Individual study time 105
SSD MED/15 - MALATTIE DEL SANGUE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi At the end of the course, the student will be able to: i) critically analyse the molecular pathology of several models of hematologic malignancies; ii) appreciate the importance of molecular pathology for improving precision medicine, molecular diagnosis and target treatment in hematologic neoplasia
Contenuti The course will deal with the molecular pathology of the main haematological malignancies, especially by analysing the topics relevant for the profession of Medical Biotechnologist. During the course, self-evaluation exercises will also be proposed, as well as additional material for in-depth study and group presentations on topics of particular interest.
Testi di riferimento Slides presented during the course; scientific papers presented at the journal clubs
Obiettivi formativi The main objective of the course is providing the basis for the molecular understanding of the pathogenesis, diagnosis and target therapy of hematologic malignancies
Prerequisiti The student is expected to have a general knowledge of the human genome, of the concepts of proto-oncogenes and tumor suppressor genes, and of the main technologies for genomic investigations
Metodi didattici A) Taught (frontal) classes with slide presentations available on the UPO DIR website (https://www.dir.uniupo.it/); B) Self-assessment tests after each class; C) Group presentations by the students on topics agreed upon with the professor
Altre informazioni Practical training Interactive journal clubs Optional activities Optional activities might be offered throughout the year
Modalità di verifica dell'apprendimento Written exam: the student will be asked to assess whether a number of statements are true/false, and to provide a brief comment and justification of the choice that has been made Oral exam: critical discussion of the written exam and of the journal club presented by the student NOTE: To access the exam the student must have filled up the course evaluation form.
Programma esteso The hematopoietic stem cell and the process of hematopoiesis; the molecular basis and rationale for molecular diagnostics and target therapy of: acute leukemias; chronic myeloid leukemia; myeloproliferative neoplasms; chronic lymphocytic leukemia; lymphoma
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Course Neoplastic and proliferative disorders: Experimental oncology
Course ID MS0471
Academic Year 2019/2020
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ISIDORO Ciro
Teachers Isidoro Ciro
CFU 7
Teaching duration (hours) 28
Individual study time 147
SSD MED/04 - PATOLOGIA GENERALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Knowing the causes and mechanisms of carcinogenesis and tumor progression. Knowing how to interpret, discuss and present the experimental laboratory results or found in literature, specifically aimed at describing and understanding the neoplastic transformation and behavior of tumor cells.
Contenuti 1. the epidemiologic relevance of Cancer; the influence of the environment and of the life style. 2. the main histopathologic characteristics of the tumours and their classification 3. the molecular alterations responsible for the malignant behaviour of tumour cells (loss of proliferative control; resistance to apoptosis; invasiveness; altered metabolism of glucose, aminoacids and fatty acids; altered proteome homeostasis); the role of autophagy and of cell death in cancer 4. the role of genetics and epigenetics in cancer development and progression; the genetic defects that predispose to cancer; genome (chromosomal translocation) alterations and gene mutations (oncogenes, oncosuppressors) associated with cancer 5. the origin and phenotypic characteristics of Cancer Stem Cells; the genetic and metabolic re-programming leading to cancer 6. the mechanisms of metastasis formation; the role of proteolytic enzymes 7. the inter-relationship between tumour cells and the tumour stroma, with particular regard to the role of cancer associated fibroblasts and of immune cells; 8. the molecular and patho-physiological mechanisms responsible for the cachexia syndrome. 9. the current techniques for Diagnosis (imaging, biomarkers) and the therapeutic treatments available (chemical chemotherapy, biomolecular therapy). 10. Novel therapeutic approach (epigenetics); novel paradigms for carcinogenesis (microbioma);
Testi di riferimento The Molecular Biology of Cancer S-Pelengaris and M. Khan (Wiley-Blackwell edition) Chapter 7 NEOPLASIA by Thomas P Stricker and V Kumar (from Robbins, The molecular Basis of Disease) A copy of the slides will be provided http://www.insidecancer.org http://www.oncolink.com/ http://www.cancer.gov
Obiettivi formativi The course will provide students with fundaments of general oncology and knowledge about cellular and molecular mechanisms involved in carcinogenesis, as well as about experimental approaches in the field of oncological research.
Prerequisiti The students must possess the basic knowledge in cell and molecular biology and in biochemistry.
Metodi didattici Students will be involved in Journal Club meetings in which scientific papers relevant for their preparation will be discussed.
Altre informazioni Students are welcome to spend a period in the laboratory of Molecular Pathology to participate in the design, execution and discussion of experiments. CFU will be assigned based on the duration of the stage.
Modalità di verifica dell'apprendimento Written test (30 minutes for 3 statements): answer TRUE/FALSE and give an explanation to support your answer in 5 (not less) to 10 (not more) lines (please, write in a legible writing). At least 2 out of 3 answers must be correct in order to be admitted to the next step (oral examination). Evaluation: 6-8/30 points for each question Oral examination (approx. 10-20 min): Discussion of the written test. Discussion of an original article dealing with a subject of your choice inherent the program of the course and published in a good journal (consult with your professor for an appropriate choice) Evaluation: 4-6/30 points (optional): 1 or 2 extra question(s). Evaluation 2-4/30, Laude The final score is agreed between the two Professors as an average of the scores obtained for the two modules. Minimal score for each module is 18/30 Maximal score is 30 + Laude/30 NOTE: To access the exam the student must have filled up the course evaluation form.
Programma esteso Tumors: generality, epidemiology, etiology. Genetic and environmental (chemical, biological and physical risk factors; viral carcinogenesis; hormones) causes of cancer. Histopathology and clinical classification of Tumors. Tumor natural history. Carcinogenesis as a multi-step process. Genetic and epigenetic events. Angiogenesis. The role of inflammation. Infiltrative growth. Epithelial mesenchimal transition. Neoplastic progression. Metastasis. Stem cell theory. Tumor-host interactions. Cancer cachexia. Metabolic systemic consequences of cancer. Molecular oncology. Oncogenes and oncosuppressor. Mechanisms of activation and inactivation. Functional consequences. Failure in neoplastic cell populations of physiological antineoplastic defense mechanisms (apoptosis, DNA repair, cell senescence; dormancy). Failure in proteome homeostasis (Ubiquitin and autophagy systems). Mechanisms of antineoplastic drug resistance. Outline of conventional and molecular therapeutic approaches to neoplastic diseases.
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Year Course ID Course Teachers SSD Curriculum Site CFU Agenda web
1 MS0452 Fertility, reproduction and endocrine system biotechnologies Marzullo Paolo, Surico Daniela MED/13, MED/40 All 4 Select module
1 MS0455 Functional Genomics Giordano Mara, Baldanzi Gianluca, Gagliardi Mara, Zucchelli Silvia MED/03, BIO/10, BIO/18 All 11 Select module
1 MS0459 Internal Medicine Castello Luigi Mario, Bellan Mattia MED/09 All 5 Lessons schedule
1 MS0495 Internship 1 NN All 4 Lessons schedule
1 MS0465 Molecular therapy Ellis Steven Robert, Colangelo Donato BIO/13, BIO/14 All 11 Select module
1 MS0468 Molecular virology Gariglio Marisa MED/07 All 6 Lessons schedule
1 MS0469 Neoplastic and proliferative disorders Gaidano Gianluca, Isidoro Ciro MED/15, MED/04 All 12 Select module
1 MS0497 Optional Activities 1 NN All 4 Lessons schedule
1 MS0491 Regenerative Medicine Pelicci Giuliana, Follenzi Antonia BIO/11, BIO/17 All 10 Select module
2 MS0451 Experimental thesis PROFIN_S All 14 Not available
2 MS0496 Internship 2 NN All 6 Lessons schedule
2 MS0461 Molecular diagnostics Boldorini Renzo Luciano, Capello Daniela, Rolla Roberta MED/08, BIO/12, MED/05 All 11 Select module
2 MS0498 Optional Activities 2 NN All 4 Lessons schedule
2 MS0473 Public health and regulatory aspects Faggiano Fabrizio, Gattei Valter, Ferrante Daniela MED/42, SECS-P/07, MED/01 All 11 Select module
2 MS0480 Transplantation and Biocompatibility Gaidano Gianluca, Cantaluppi Vincenzo, Chiocchetti Annalisa, Boccafoschi Francesca, Rimondini Lia, Mantovani Diego MED/15, MED/14, MED/04, BIO/16, MED/28, BIO/11 All 7 Select module
Data synched: 04/07/2020, 01:10