Medical Biotechnology

Academic program

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Course Public health and regulatory aspects: Public Health
Course ID MS0474
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FAGGIANO Fabrizio
Teachers Faggiano Fabrizio
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD MED/42 - IGIENE GENERALE E APPLICATA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
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Course Public health and regulatory aspects: Innovation and Patents
Course ID MS0475
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GATTEI VALTER
Teachers Gattei Valter
CFU 1
Teaching duration (hours) 8
Individual study time 17
SSD SECS-P/07 - ECONOMIA AZIENDALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
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Course Public health and regulatory aspects: Biomedical statistics
Course ID MS0476
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FERRANTE Daniela
Teachers Ferrante Daniela
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/01 - STATISTICA MEDICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Knowledge and understanding: to go in depth of statistical methods for the analysis of clinical data. Ability to apply knowledge and understanding: translating theory into practice by concretely working at the solutions to exercises. Decision-making autonomy: acquiring autonomy in decision to conduct the analysis of data obtained from surveys. Communication skills: ability to communicate knowledge with clarity, scientific and methodological rigour. Learning skills: dealing with the information obtained from clinical studies with the objective of collecting and organizing data and correctly interpreting the information.
Contenuti The course consists in a first part with topics related to descriptive statistics and in a second part that will concern the inferential statistics.
Testi di riferimento W.W. Daniel, CL. Cross Biostatistics, Tenth edition. John Wiley & Sons Rosner B. Fundamentals of Biostatistics. Eighth edition. Brooks/Cole Pub Co
Obiettivi formativi The course aims to: - provide methodological issues related to descriptive and inferential statistics - select the appropriate statistical method to summarize study data - interpretation of the results
Prerequisiti Basic knowledge in mathematics
Metodi didattici Lectures, Classroom exercises
Altre informazioni The Course material is available in the Moodle platform.
Modalità di verifica dell'apprendimento Written exam. The exam aims to verify knowledge and skills acquired by the student through the solution of exercises and open questions concerning the course programme
Programma esteso The frequency distribution Measures of Central Tendency and dispersion Properties of Probability Probability distribution: the Poisson and normal distribution Sampling distribution Distribution of the sample mean Confidence interval Hypothesis testing: a single population mean, the difference between two population means, a single population proportion Type I and type II error and the power of a test Chi-square test Odds ratio The correlation coefficient and the regression model Survival analysis
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Course Transplantation and Biocompatibility
Course ID MS0480
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teachers Patriarca Andrea, Cantaluppi Vincenzo, Chiocchetti Annalisa, Boccafoschi Francesca, Rimondini Lia, Mantovani Diego
CFU 7
SSD MED/15 - MALATTIE DEL SANGUE, MED/14 - NEFROLOGIA, MED/04 - PATOLOGIA GENERALE, BIO/16 - ANATOMIA UMANA, MED/28 - MALATTIE ODONTOSTOMATOLOGICHE, BIO/11 - BIOLOGIA MOLECOLARE
Course type Attività formativa integrata
Course mandatoriety OBB
Year 2
Period Primo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0481Hematopoietic stem cell biology and transplantation MED/15 - MALATTIE DEL SANGUE Patriarca Andrea
MS0482Biotechnology in kidney transplantation MED/14 - NEFROLOGIA Cantaluppi Vincenzo
MS0483Transplant immunology MED/04 - PATOLOGIA GENERALE Chiocchetti Annalisa
MS0484Materials Biocompatibility BIO/16 - ANATOMIA UMANA Boccafoschi Francesca
MS0485Nanomaterials MED/28 - MALATTIE ODONTOSTOMATOLOGICHE Rimondini Lia
MS0493Molecular strategies for improving materials biocompatibility BIO/11 - BIOLOGIA MOLECOLARE Mantovani Diego
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Course Transplantation and Biocompatibility: Hematopoietic stem cell biology and transplantation
Course ID MS0481
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader PATRIARCA ANDREA
Teachers Patriarca Andrea
CFU 1
Teaching duration (hours) 8
Individual study time 19
SSD MED/15 - MALATTIE DEL SANGUE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
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Course Transplantation and Biocompatibility: Biotechnology in kidney transplantation
Course ID MS0482
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CANTALUPPI Vincenzo
Teachers Cantaluppi Vincenzo
CFU 1
Teaching duration (hours) 6
Individual study time 19
SSD MED/14 - NEFROLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
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Course Transplantation and Biocompatibility: Transplant immunology
Course ID MS0483
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CHIOCCHETTI Annalisa
Teachers Chiocchetti Annalisa
CFU 2
Teaching duration (hours) 8
Individual study time 42
SSD MED/04 - PATOLOGIA GENERALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Students should understand alloreactivity and be able to describe why the immune system aggresses the graft
Contenuti The course describes different types of graft and graft rejection and their immunological mechanisms HLA typing and treatments are also briefly treated
Testi di riferimento No reference book
Obiettivi formativi Students will understand the immunological mechanisms underlying graft rejection, GVHD and immunological strategies to prevent rejection
Prerequisiti Basic knowledge of immunology are required
Metodi didattici Frontal lessons with slides and active learning
Altre informazioni none
Modalità di verifica dell'apprendimento The exam consists of 3 statements (true or false) + the explanation
Programma esteso types of graft - alloreactivity - hyperacute rejection - acute rejection - chronic rejection treatments to prevent rejection - compatibility typing (Microtoxicity test and MLC) - Graft versus host disease - malignencies post-transplantation -innovative therapeutic approaches to prevent rejection
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Course Transplantation and Biocompatibility: Materials Biocompatibility
Course ID MS0484
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BOCCAFOSCHI FRANCESCA
Teachers Boccafoschi Francesca
CFU 1
Teaching duration (hours) 4
Individual study time 21
SSD BIO/16 - ANATOMIA UMANA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Testi di riferimento powerpoint presentations and videos furnished via DIR
Obiettivi formativi Provide the basis for understanding the biocompatibility of materials for biomedical use
Prerequisiti Cell biology, immunology, molecular biology
Metodi didattici Frontal lectures
Modalità di verifica dell'apprendimento Written exam
Programma esteso 1.Biocompatibility: basic concepts 2.Polymers: biomechanical characteristics and applications in the biomedical field
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Course Transplantation and Biocompatibility: Nanomaterials
Course ID MS0485
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader RIMONDINI Lia
Teachers Rimondini Lia
CFU 1
Teaching duration (hours) 4
Individual study time 21
SSD MED/28 - MALATTIE ODONTOSTOMATOLOGICHE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Knowledge and understanding of the main properties of matter at nanoscale and the potential application in Medicine. Knowledge of the main methods to manage and characterize matter at the nanoscale
Contenuti See Course program below
Testi di riferimento Papers, reviews, chapters provided during classes
Obiettivi formativi Knowledge and understanding of the main properties of the matter at the nanoscale and their characterization. Knowledge and understanding of the main properties of the nanomaterials used in Medicine and their applications. Development of skills addressed to plan R&D on nanomaterials for Medical applications.
Prerequisiti Fundamentals of chimics and physics
Metodi didattici Lectures
Altre informazioni None
Modalità di verifica dell'apprendimento Written test: Statments assessment and comments
Programma esteso Definition of nanomaterials; properties of the materials at the nanoscale; methods to characterize nanomaterials; methods for nanomaterials manufacturing; esample of nanomaterials used for diagnosis and treatment of various diseases (including cancer); nanocarriers for drugs, nanomaterials for tissue regeneration, toxicology of nanomaterials.
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Course Transplantation and Biocompatibility: Molecular strategies for improving materials biocompatibility
Course ID MS0493
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader MANTOVANI DIEGO
Teachers Mantovani Diego
CFU 1
Teaching duration (hours) 8
Individual study time 17
SSD BIO/11 - BIOLOGIA MOLECOLARE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Primo Semestre
Grading type V
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Course Molecular diagnostics
Course ID MS0461
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teachers Boldorini Renzo Luciano, Capello Daniela, Rolla Roberta
CFU 11
SSD MED/08 - ANATOMIA PATOLOGICA, BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA, MED/05 - PATOLOGIA CLINICA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 2
Period Secondo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0462Pathologic basis of diseases MED/08 - ANATOMIA PATOLOGICA Boldorini Renzo Luciano
MS0463Clinical applications of Molecular Biology BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA Capello Daniela
MS0464Clinical Pathology MED/05 - PATOLOGIA CLINICA Rolla Roberta
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Course Molecular diagnostics: Pathologic basis of diseases
Course ID MS0462
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BOLDORINI Renzo Luciano
Teachers Boldorini Renzo Luciano
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/08 - ANATOMIA PATOLOGICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
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Course Molecular diagnostics: Clinical applications of Molecular Biology
Course ID MS0463
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader CAPELLO Daniela
Teachers Capello Daniela
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 2
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
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Course Molecular diagnostics: Clinical Pathology
Course ID MS0464
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader ROLLA Roberta
Teachers Rolla Roberta
CFU 1
Teaching duration (hours) 4
Individual study time 21
SSD MED/05 - PATOLOGIA CLINICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 2
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Contenuti This course provides an overview of the current knowledge in pharmacogenetics.
Testi di riferimento Teaching Material provided by the lecturer. Lecturer's Monograph.
Obiettivi formativi The aim of the present course is to provide knowledge about the influence of genetics on drug therapy and about the validity and utility of pharmacogenomic tests in the clinical setting.
Prerequisiti Thorough knowledge of Molecular Biology and Genetics (basics of genomics as well as methods to identify mutations).
Metodi didattici PowePoint presentation. Teaching Network on DIR Website. Frontal lessons.
Modalità di verifica dell'apprendimento Multiple choice test
Programma esteso One of the great challenges in drug development and therapy is maximizing therapeutic response while avoiding adverse effects. Advances in genetic knowledge, gained through sequencing, have identified heritable genetic variants that predict drug response. Pharmacogenetics is the study of inherited genetic differences in drug metabolic pathways which can affect individual responses to drugs, both in terms of therapeutic effect as well as adverse effects/ toxicity. The use of these genetic informations can play a major role in clinical practice to predict the right choice and dose of medications for individuals, thus providing a scientific basic for “personalized medicine”. This is particularly important in the case of anticancer drugs whose the therapeutic index is very narrow and a large proportion of patients do not respond. Although the knowledge on an individual’s DNA sequence as an integral determinant of drug therapy has not yet become standard clinical practice, however several genetics recommendations/guidelines for physicians have been developed and are highlighted in the present course. In particular, the course explores the pharmacogenetic clinical utility in: •anticancer drugs (tamoxifen & breast cancer) •treatment of chronic pain (codeine) •antipsychotic therapy •antiplatelet therapy (clopidogrel) •oral anticoagulant therapy (warfarin)
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Course Scientific English Course for the thesis
Course ID MS2001
Academic Year 2020/2021
Year of rule 2019/2020
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader KALININA IRINA
Teachers Kalinina Irina
SSD NN -
Course type Attività formativa monodisciplinare
Course category D
Year 2
Period Secondo Semestre
Site NOVARA
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi By the end of the course, students are expected to have gained a good understand of the scientific writing principles applied to the work on their thesis.
Contenuti The course is designed to provide students with an oppurtunity to improve their knowledge of the conventions of academic and scientific English. The syllabus aims to cover some of the areas of scientific communication that students should master in order to successfully promote their research and write their thesis. Students will also get a chance to improve their academic English vocabulary and grammar.
Testi di riferimento The required book for the course is by Wallwork, Adrian, "English for Writing Research Papers". Order a copy online or call/write to LIBRERIA SCIENTIFICA UNIVERSITARIA RESCALLI (VIA DEI MILLE 1 D, 28100 Novara) at 0321613113/ scientificarescalli@gmail.com - the bookshop might offer you a discount. Optional books (it's not necessary to buy those unless you want to) R.E.Taylor (2011) Medical Writing. A Guide for Clinicians, Educators and Researchers, 2nd edition, Springer, Ney York R.A.Day, N.Sakaduski (2011) Scientific English. A Guide for Scientists and Other Professionals. 3rd edition, Greenwood, Oxford. J.D.Lester, J.D.Jester Jr (2015) Writing Research Papers. A Complete Guide. 15th edition. Pearson. H.Glasman-Deal (2010) Science Research Writing for Non-Native Speakers of English. Imperial College Press, London. A.Wallwork (2016) English for Academic Research: Grammar. Springer U.Eco (2015) How to Write a Thesis. The MIT Press. P.M.S. O’Brien, F.B. Pipkin (2017) Introduction to Research Methodology for Specialists and Trainees. 3rd edition. Cambridge University Press, Cambridge
Obiettivi formativi Learning the tools and communication conventions in the sciences provides multiple benefits to students. The course aims to teach the fundamentals of effective scientific writing from multiple perspectives. Instruction will focus primarily on the process of writing the thesis.
Prerequisiti Students are expected to have at least a B2 level in English. The attendance requirement for the course is 70% minimum.
Metodi didattici Lectures: the lectures will provide theoretical guidelines and principles as well as practical examples from scientific texts and exercises to teach students how to apply the guidelines to their own work.
Altre informazioni Please connect to the DIR page of the course: https://www.dir.uniupo.it/course/view.php?id=12643#section-1
Modalità di verifica dell'apprendimento The exam will be computer-based and consist of various questions (multiple choise, true/false, fill-in, etc.). The questions will pertain to the course/lecture contents.
Programma esteso Lesson 1 1 Introduction into scientific writing. Choosing your topic. 2 Structuring a Sentence: Word Order 3 Titles Lesson 2 4 Abstracts 5 Structuring Paragraphs 6 Being Concise and Removing Redundancy Lesson 3 7 Introduction and research proposal 8 Breaking Up Long Sentences 9 Avoiding Ambiguity, Repetition, and Vague Language Lesson 4 10 Review of the Literature 11 Clarifying Who Did What 12 Plagiarism and Paraphrasing Lesson 5 13 Methods 14 Highlighting Your Findings 15 Discussing Your Limitations Lesson 6 16 Results 17 Discussion 17 Hedging and Criticising Lesson 7 19 Conclusions 20 The Final Check
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Course Neoplastic and proliferative disorders
Course ID MS0469
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teachers Gaidano Gianluca, Isidoro Ciro
CFU 12
SSD MED/15 - MALATTIE DEL SANGUE, MED/04 - PATOLOGIA GENERALE
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Primo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0470Hematoncology MED/15 - MALATTIE DEL SANGUE Gaidano Gianluca
MS0471Experimental oncology MED/04 - PATOLOGIA GENERALE Isidoro Ciro
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Course Neoplastic and proliferative disorders: Hematoncology
Course ID MS0470
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teaching leader GAIDANO Gianluca
Teachers Gaidano Gianluca
CFU 5
Teaching duration (hours) 40
Individual study time 85
SSD MED/15 - MALATTIE DEL SANGUE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
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Course Neoplastic and proliferative disorders: Experimental oncology
Course ID MS0471
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teaching leader ISIDORO Ciro
Teachers Isidoro Ciro
CFU 7
Teaching duration (hours) 28
Individual study time 147
SSD MED/04 - PATOLOGIA GENERALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Knowing the causes and mechanisms of carcinogenesis and tumor progression. Knowing how to interpret, discuss and present the experimental laboratory results or found in literature, specifically aimed at describing and understanding the neoplastic transformation and behavior of tumor cells.
Contenuti 1. the epidemiologic relevance of Cancer; the influence of the environment and of the life style. 2. the main histopathologic characteristics of the tumours and their classification 3. the molecular alterations responsible for the malignant behaviour of tumour cells (loss of proliferative control; resistance to apoptosis; invasiveness; altered metabolism of glucose, aminoacids and fatty acids; altered proteome homeostasis); the role of autophagy and of cell death in cancer 4. the role of genetics and epigenetics in cancer development and progression; the genetic defects that predispose to cancer; genome (chromosomal translocation) alterations and gene mutations (oncogenes, oncosuppressors) associated with cancer 5. the origin and phenotypic characteristics of Cancer Stem Cells; the genetic and metabolic re-programming leading to cancer 6. the mechanisms of metastasis formation; the role of proteolytic enzymes 7. the inter-relationship between tumour cells and the tumour stroma, with particular regard to the role of cancer associated fibroblasts and of immune cells; 8. the molecular and patho-physiological mechanisms responsible for the cachexia syndrome. 9. the current techniques for Diagnosis (imaging, biomarkers) and the therapeutic treatments available (chemical chemotherapy, biomolecular therapy). 10. Novel therapeutic approach (epigenetics); novel paradigms for carcinogenesis (microbioma);
Testi di riferimento The Molecular Biology of Cancer S-Pelengaris and M. Khan (Wiley-Blackwell edition) Chapter 7 NEOPLASIA by Thomas P Stricker and V Kumar (from Robbins, The molecular Basis of Disease) A copy of the slides will be provided http://www.insidecancer.org http://www.oncolink.com/ http://www.cancer.gov
Obiettivi formativi The course will provide students with fundaments of general oncology and knowledge about cellular and molecular mechanisms involved in carcinogenesis, as well as about experimental approaches in the field of oncological research.
Prerequisiti The students must possess the basic knowledge in cell and molecular biology and in biochemistry.
Metodi didattici Students will be involved in Journal Club meetings in which scientific papers relevant for their preparation will be discussed.
Altre informazioni Students are welcome to spend a period in the laboratory of Molecular Pathology to participate in the design, execution and discussion of experiments. CFU will be assigned based on the duration of the stage.
Modalità di verifica dell'apprendimento Written test (30 minutes for 3 statements): answer TRUE/FALSE and give an explanation to support your answer in 5 (not less) to 10 (not more) lines (please, write in a legible writing). At least 2 out of 3 answers must be correct in order to be admitted to the next step (oral examination). Evaluation: 6-8/30 points for each question Oral examination (approx. 10-20 min): Discussion of the written test. Discussion of an original article dealing with a subject of your choice inherent the program of the course and published in a good journal (consult with your professor for an appropriate choice) Evaluation: 4-6/30 points (optional): 1 or 2 extra question(s). Evaluation 2-4/30, Laude The final score is agreed between the two Professors as an average of the scores obtained for the two modules. Minimal score for each module is 18/30 Maximal score is 30 + Laude/30 NOTE: To access the exam the student must have filled up the course evaluation form.
Programma esteso Tumors: generality, epidemiology, etiology. Genetic and environmental (chemical, biological and physical risk factors; viral carcinogenesis; hormones) causes of cancer. Histopathology and clinical classification of Tumors. Tumor natural history. Carcinogenesis as a multi-step process. Genetic and epigenetic events. Angiogenesis. The role of inflammation. Infiltrative growth. Epithelial mesenchimal transition. Neoplastic progression. Metastasis. Stem cell theory. Tumor-host interactions. Cancer cachexia. Metabolic systemic consequences of cancer. Molecular oncology. Oncogenes and oncosuppressor. Mechanisms of activation and inactivation. Functional consequences. Failure in neoplastic cell populations of physiological antineoplastic defense mechanisms (apoptosis, DNA repair, cell senescence; dormancy). Failure in proteome homeostasis (Ubiquitin and autophagy systems). Mechanisms of antineoplastic drug resistance. Outline of conventional and molecular therapeutic approaches to neoplastic diseases.
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Course Fertility, reproduction and endocrine system biotechnologies
Course ID MS0452
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teachers Marzullo Paolo, Surico Daniela
CFU 4
SSD MED/13 - ENDOCRINOLOGIA, MED/40 - GINECOLOGIA E OSTETRICIA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Primo Semestre
Grading type V
Modules
Course ID Course SSD Teachers Agenda web
MS0453Endocrinology and Metabolism MED/13 - ENDOCRINOLOGIA Marzullo Paolo
MS0454Biotechnology of reproduction MED/40 - GINECOLOGIA E OSTETRICIA Surico Daniela
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Course Fertility, reproduction and endocrine system biotechnologies: Endocrinology and Metabolism
Course ID MS0453
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teaching leader MARZULLO Paolo
Teachers Marzullo Paolo
CFU 2
Teaching duration (hours) 10
Individual study time 40
SSD MED/13 - ENDOCRINOLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Testi di riferimento Eric I. Felner, Guillermo E. Umpierrez. Endocrine pathophysiology. Lippincott Williams And Wilkins (12/2013)
Obiettivi formativi Aims of the Course are to provide the Student with broad knowledge on the theoretical, technical and clinically-oriented aspects of the endocrine system and main endocrinopathies, to enhance awareness of the basic sciences and research techniques underpinning endocrinology and metabolism, as well as to transfer information on latest Literature evidence and critical appraisal of novel biotechniques applicable to this discipline.
Prerequisiti Fundamental knowledge in the areas of Anatomy, Physiology and General Pathology preliminary to learning Endocrinology.
Metodi didattici Power-point presentations. Scientific articles from peer-reviewed journals.
Modalità di verifica dell'apprendimento The final exam for the course of Fertility, Reproduction and Endocrine System Biotechnologies will be done in a single session according to the prescheduled yearly timetable. It will consist of a written exam, and will include two consecutive tests, one for each subject of the course with lecturers present during the tests. Each test will consist of three statements queries, each statement will require a 5-to-10 lines answer. The maximum time allowed for each test will be 30 mins (one hour total). Test results will be available the day after the exam.
Programma esteso The endocrine system. The importance of biotechnologies for classification, characterization, understanding and treatment of endocrine diseases. Pituitary axes, hypothalamo-pituitary neoplasms, genetic disorders. Pharmaceutical targeting, combined approaches. Neuroendocrine tumors. Pharmaceutical approach. Molecular therapies. Thyroid dysfunctions, sporadic and familial diseases, thyroid nodules and tumors. Adrenal physiology, glucocorticoid and mineralcorticoid-related diseases, sporadic and genetic disorders of the adrenal medulla. Pathophysiology and causes of obesity and diabetes mellitus, endocrine disrupting chemicals, neuroendocrine control of energy expenditure and appetite, metabolic syndrome, adipocytes, central and peripheral pharmaceutical targets. Gonadal dysfunctions, hypogonadisms, policystic ovary syndrome
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Course Fertility, reproduction and endocrine system biotechnologies: Biotechnology of reproduction
Course ID MS0454
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teaching leader SURICO Daniela
Teachers Surico Daniela
CFU 2
Teaching duration (hours) 12
Individual study time 38
SSD MED/40 - GINECOLOGIA E OSTETRICIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Be familiar with causes of male and female infertility, ICSI/FIVET procedure, cryopreservation and pre-implant diagnosis techniques actually disposable.
Contenuti Female and male infertility and diagnostic program for infertile couple. Assisted fertilization technologies ( IVF and ICSI procedure)Basic aspects of human implantation. Future strategies to improve implantation in patients undergoing ART Variables involved in the outcome of cryopreservation
Testi di riferimento Human fertility: methods and protocols. Editors: Rosenwaks, Zev, Wassarman, Paul M., 2014ISBN 978-1-4939-0659-8
Obiettivi formativi At the end of the course, the student should be able to describe causes of male and famale infertility, ICSI/FIVET procedure, cryopreservation and pre-implant diagnosis techniques actually disposable.
Prerequisiti Basic knowledge of pelvica male and female anatomy and ovarian cycle
Metodi didattici power point presentation
Modalità di verifica dell'apprendimento The exam is inserted in a test that includes all the activities partecipating at the integrated course. Evaluation of the student consists in a written test formed by 6 questions. The student has to answer true or false giving a brief explanation.The student will have 60 minutes to complete the esam.
Programma esteso Female and male infertility: causes and risk factors. Physiology of aging ovary and checking for ovarian reserve Diagnostic program for infertile couple Development of assisted fertilization technologies (IVF in animals, IVF in human ICSI in animals, ICSI in human) In vitro production of functional gametes Basic physiology of oogenesis Ovarian stimulation. IVF and ICSI procedureBasic aspects of human implantation. Current strategies to improve implantation in patients undergoing ART .Improving endometrial receptivity in IVF patients. Selecting chromosomally normal embryos in patients affected by recurrent miscarriages. Future strategies to improve implantation in patients undergoing ART Variables involved in the outcome of cryopreservation. Clinical results, survival, fertilization of cryopreserved oocytes, embryos and sperm
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Course Internal Medicine
Course ID MS0459
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teachers Bellan Mattia
CFU 5
Teaching duration (hours) 20
Individual study time 105
SSD MED/09 - MEDICINA INTERNA
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento English
Testi di riferimento For consultation: Harrison’s Principles of Internal Medicine - 17th edition - New York: McGraw-Hill, 2009. References on specific topics will be provided during the course.
Obiettivi formativi The aim of the course is to give notions of methodology relating to the main biotechnological applications in Internal Medicine. In particular, the course will transmit skills on the growing role of biotechnology applications both in the diagnostic and in the therapeutic approach to different acute and chronic diseases. The topics that will be object of the lessons have been selected to give examples of diseases characterized by educational relevance in the description of the diagnostic approach strategies (genetic diseases on one hand but also the development of diagnostic strategies in the fight against doping in sport) in the study of pathophysiology (diabetes mellitus, hypercalcemia) and in the therapeutic approach (evolution of the treatment of diabetes mellitus, viral hepatitis and autoimmune diseases such as rheumatoid arthritis). The course will also show some examples of the development of drugs obtained by recombinant DNA techniques and the development of innovative therapeutic strategies such as stem cell transplantation and gene therapy in different conditions.
Prerequisiti The student should possess basic knowledge about the Human Anatomy, Biochemistry, Physiology, Pharmacology and General Pathology.
Metodi didattici Lectures with supporting slides
Altre informazioni The student may request to attend the laboratory of Emergency Medicine and Internal Medicine, with targets to be agreed with the teacher.
Modalità di verifica dell'apprendimento The final test will consist of multiple choice quiz or oral exam at the discretion of the teacher based on the number of candidates.
Programma esteso - Aspects of the biotechnological treatment of viral hepatitis - The biological basis of treatment of autoimmune diseases - The biological basis of treatment of diabetes mellitus - Calcium-Phosphate metabolism: pathophysiology and biotechnological implications - The physiopathology of cardiovascular disease; novel target thrapies - Biotechnological implications in infectious diseases with a specific focus on SARS-CoV-2 infection; - The physiopathology of sepsis; - The mechanisms of platelets aggregation and coagulation.
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Course Molecular therapy
Course ID MS0465
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teaching leader COLANGELO Donato
Teachers Ellis Steven Robert, Colangelo Donato
CFU 11
SSD BIO/13 - BIOLOGIA APPLICATA, BIO/14 - FARMACOLOGIA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi The students are expected to acquire the knowledge of the main topics of Pharmacology and Biotechnological applications and to approach with criticism the scientific problems of these topics, together with the ability to set up experimental designs in vitro and in vivo.
Contenuti The course integrates Pharmacology and Biotechnological applications. It will focus mainly on the rational bases for the development of selected classes of target –designed drugs. The course will also analyze relevant molecular and diagnostic properties useful for the preclinical development of new biotech drugs.
Testi di riferimento The learning material and specific texts will be discussed together with the students. The professors will furnish to the students some didactic material as pdf and ppt format before the lessons. Pleas consult the specific D.I.R website of Prof. Colangelo and Prof. Ellis. Access keywords will be given to the students during the first lesson.
Obiettivi formativi The course is intended to provide specific knowledge of pharmacology and biotechnological applications of selected drugs developed with biotech methodologies.
Prerequisiti Basic knowledges of Immunology, Anatomy, Physiopathology and Pathology.
Metodi didattici Frontal Lessons. All teaching activities will take place in front of the classroom. The learning material will be furnished to the students before the lessons as pdf. It can be found on the specific D.I.R website of Prof. Colangelo and Prof. Ellis with a keyword that will be given to the students during the first lesson.
Altre informazioni The teachers will meet the students on specific days. Please refer to each specific course description. We suggest preventive email reservation.
Modalità di verifica dell'apprendimento Multiple choice tests. For the assessment of the student preparation, a numerical grading will be adopted. It that will range between 18 (minimum) and 30 with laude (maximum), according to the preparation of the student. In detail: From 18 to 21: the student has a limited preparation and a limited ability to allocate the information learned in the course in a scientific landscape. From 22 to 24: the student has a sufficient but partial preparation and a limited ability to allocate the information learned in the course in a scientific landscape. From 25 to 27: the student has a good preparation and sufficient ability to allocate the information learned in the course in a scientific landscape. From 28 to 30: the student has an optimal preparation and a good ability to allocate the information learned in the course in a scientific landscape. 30 laude: the student has an excellent preparation.
Programma esteso The course will focus on the analysis of the rational bases for the development of selected classes of target –designed drugs for clinical use. Furthermore, the course integrates basic pharmacology and biotech applications for preclinical development of new biotech drugs.
Modules
Course ID Course SSD Teachers Agenda web
MS0466Biotechnological applications BIO/13 - BIOLOGIA APPLICATA Ellis Steven Robert
MS0467Pharmacology BIO/14 - FARMACOLOGIA Colangelo Donato
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Course Molecular therapy: Biotechnological applications
Course ID MS0466
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teaching leader ELLIS Steven Robert
Teachers Ellis Steven Robert
CFU 6
Teaching duration (hours) 36
Individual study time 114
SSD BIO/13 - BIOLOGIA APPLICATA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
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Course Molecular therapy: Pharmacology
Course ID MS0467
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum THE MOLECULAR BASIS OF DISEASE
Teaching leader COLANGELO Donato
Teachers Colangelo Donato
CFU 5
Teaching duration (hours) 25
Individual study time 100
SSD BIO/14 - FARMACOLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento LANGUAGE OF THE COURSE: English
Risultati di apprendimento attesi EXPECTED LEARNING OUTCOMES: The students are expected to acquire the knowledge of the main topics of Pharmacology and to approach with criticism the scientific problems of this topic, together with the ability to set up an experimental design related to Pharmacology in vitro and in vivo. NOTE: To access the exam the student must have filled up the course evaluation form.
Contenuti COURSE CONTENT: The course will focus on the analysis of the rational bases for the development of selected classes of target –designed drugs, also considering their pharmacokinetics and pharmacodynamic aspects for a clinical use. Furthermore, the course integrates basic pharmacology with clinic properties. Relevant drugs will be also analyzed in order to point out relevant molecular and diagnostic properties useful for the preclinical development of new biotech drugs.
Testi di riferimento REFERENCE TEXTS: The pharmacological bases of therapy, Goodman e Gilman, XI, Mc Graw-Hill Pharmacology, Rang H.P., Dale M.M., Ritter J.M e Moore P.K., Editor Ambrosiana
Obiettivi formativi LEARNING OBJECTIVES OF THE COURSE The course is intended to provide specific knowledge of pharmacologic constants that rule the therapeutic properties of selected drugs developed with biotech methodologies. Furthermore, the course intends to provide the bases for the setting of in vivo and in vitro studies conduction and for the interpretation of the results of new molecules with therapeutic potential.
Prerequisiti STUDENT BACKGROUND REQUIRED: Basic knowledges of Anatomy, Physiopathology and Pathology.
Metodi didattici PROVISION OF THE COURSE AND TOOLS: Frontal Lessons. All teaching activities will take place in front of the classroom. The learnig material will be furnished to the students before the lessons as pdf. It can be found on the specific D.I.R website with a keyword that will be given during the first lesson.
Altre informazioni OFFICE HOURS: The teacher will meet the students on every Tuesday 2-6 p.m., best after email reservation.
Modalità di verifica dell'apprendimento FINAL ASSESSMENT OF THE COURSE: Multiple choice tests. For the assessment of the student preparation, a numerical grading will be adopted. It that will range between 18 (minimum) and 30 with laude (maximum), according to the preparation of the student. In detail: From 18 to 21: the student has a limited preparation and a limited ability to allocate the information learned in the course in a scientific landscape. From 22 to 24: the student has a sufficient but partial preparation and a limited ability to allocate the information learned in the course in a scientific landscape. From 25 to 27: the student has a good preparation and sufficient ability to allocate the information learned in the course in a scientific landscape. From 28 to 30: the student has an optimal preparation and a good ability to allocate the information learned in the course in a scientific landscape. 30 laude: the student has an excellent preparation.
Programma esteso DETAILED COURSE PROGRAM: The course will focus on the analysis of the rational bases for the development of selected classes of target –designed drugs, also considering their pharmacokinetics and pharmacodynamic aspects for a clinical use. Furthermore, the course integrate basic pharmacology with clinic properties. Relevant drugs will be also analyzed in order to point out relevant molecular and diagnostic properties useful for the preclinical development of new biotech drugs.
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Course Structural genomics: Metagenomics
Course ID MS1867
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum SYSTEM BIOMEDICINE
Teaching leader FAVERO FRANCESCO
Teachers Favero Francesco
CFU 2
Teaching duration (hours) 12
Individual study time 38
SSD BIO/18 - GENETICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Expected learning results Students must be able to understand and criticize scientific publications of metagenomics and theoretically set up correctly a pipeline for the analysis of metagenomics data.
Contenuti Contents The course is constituted of an introduction to the principal protocols of metagenomics study, applied in particular to the study of the human gut microbiota. The course is focused on the main Next Generation Sequencing (NGS) techniques for the study of microbiota and metagenomics (i.e. 16S and shotgun metagenomics sequencing), with a few hints of metatranscriptomics and metaproteomics.
Testi di riferimento Didactical Materials Slides presented during the course; Suggested book: “Metagenomics for Microbiology” by Jacques Izard and Maria Rivera.
Obiettivi formativi Learning objectives of the course The main objective of the course is providing the basis for the understanding of the principal methods used for the study of the microbiota using NGS technology and to perform a bioinformatics analysis of data coming from a NGS metagenomics study.
Prerequisiti Prerequisites Basal knowledge of molecular biology and genetics.
Metodi didattici Didactical methods Lectures in classroom.
Modalità di verifica dell'apprendimento Final assesment of the course Oral exam that consists in the presentation of a research article chosen by a list of suggested articles presented during the lessons.
Programma esteso Extended program of the course • Review of the principal platforms of Next Generation Sequencing (NGS) and formats format of the files of NGS data (Fastq, Bam). • Introduction of metagenomics and metatranscriptomics and their potential applications on human physiopathology. • 16S sequencing: pro and cons and applications. • Shotgun Sequencing: pro and cons and applications. • Post-processing of de novo assembly genomes: quality controls and further analyses (metaproteomics). • Metatranscriptomics: analogies and differences compared to metagenomics (hints). • Hints on the principal tools for the analysis of metagenomics data.
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Course Molecular virology
Course ID MS1881
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum SYSTEM BIOMEDICINE
Teaching leader GARIGLIO Marisa
Teachers Gariglio Marisa
CFU 6
Teaching duration (hours) 30
Individual study time 120
SSD MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Grading type V
Lingua insegnamento English
Contenuti The general content of the course is the interplay between viruses and their host organisms
Testi di riferimento The recommended textbooks are: Brooks “Jawetz, Melnick, & Adelberg's Medical Microbiology” Levinson “Review of Medical Microbiology and Immunology” Ryan “Sherris Medical Microbiology” ON ARRIVAL: David M Knipe,Peter Howley“Fields Virology” 6th edition Additional material can be found at: http://www.virology.ws/course/
Obiettivi formativi Understanding the interplay between viruses and their host organisms giving to students a view of innate defenses and viral pathogenesis focusing on medically important viruses.
Prerequisiti The students are expected to know basic virology and fundamentals of molecular and cellular biology.
Metodi didattici All the slides of each lesson will be available to the students.
Modalità di verifica dell'apprendimento 6 questions with true or false answer and explanation An oral exam will be following in order to discuss and complete (if necessary) the written exam.
Programma esteso The course deals with basic virology and virological terminology. The effect of virus infection at organism level is addressed. The focus of the course is on molecular virology, especially on virus replication in the infected cell and the molecular mechanisms of this. A selected set of relevant human viruses, differing in their genome structure (RNA versus DNA, circular versus linear) and in their virion architecture will be discussed, the content of the course being specialised with respect to molecular biology, molecular genetics, recombinational events, virus-host interactions and innate immunity. The focus is on medically important viruses, such as tumour viruses, hepatitis viruses, HIV and herpesviruses.
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Course Big Data Analysis
Course ID MS1864
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum SYSTEM BIOMEDICINE
Teaching leader CORA' Davide
Teachers Cora' Davide
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD BIO/11 - BIOLOGIA MOLECOLARE
Course type Attività formativa monodisciplinare
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi It is expected an increased knowledge-based and personalized judgement capability on basic applied bioinformatics, in particular concerning the human genome and biomedical applications. Being able to understand independently and critically the content of research articles concerning bioinformatics and systems biology topics and results.
Contenuti The course will provide an introduction to some examples of concepts and instruments at the foundation of Big Data Analysis for bioinformatics and genomics, in the framework of human health and cancer in particular. Students will gain an introduction to the theory and practice of genomic and protein sequence databases along with tools for retrieving and interpreting functional and annotation data for the human genome. Basic knowledge on new generation sequencing and related bioinformatics data analysis tools will be introduced, focusing on transcriptomes and cancer genomics. Basics of the statistical language R will be discussed. In the final part, an introduction to the concepts of complex systems, network theory and system biology will be considered also through the discussion of research articles.
Testi di riferimento Scientific papers and slides, provided during the lessons, will represent the main teaching materials. Further suggested books: Arthur Lesk, “Introduction to Bioinformatics”, Oxford University Press Arthur Lesk, “Introduction to Genomics”, Oxford University Press Deonier et al., “Computational Genome Analysis – An introduction”, ed Springer Andreas D. Baxevanis, Gary D. Bader, David S. Wishart, “Bioinformatics – fourth edition”, ed. John Wiley & Sons
Obiettivi formativi Provide a basic view of the most commonly used bioinformatics and systems biology tools in the framework of “Big Data” analysis for human health, with a focus for cancer genomics. Provide basic skills for reading and interpreting articles including bioinformatics content.
Prerequisiti Basic knowledge in molecular biology and genetics.
Metodi didattici Lectures in classroom and computer classroom.
Modalità di verifica dell'apprendimento In-itinere written reports for a series of assignments (computer homeworks) and a final oral exam are planned. The final oral exam will consist of questions concerning the development of the homeworks and the corresponding reports, plus the presentation of one suggested research article.
Programma esteso Review of basic concepts on the structure, organization and functioning of the human genome. The Ensembl database, description and operation. Structure of a human gene in Ensembl, sequence information retrieval, protein structure, functional and clinical annotations. The UCSC browser genome, description and operation. UCSC structure and annotation of a human gene, retrieval of functional and expression information. Introduction to NGS Sequencing. Illumina sequencing methodology. Introduction to RNA-seq methodology. Design of an RNA-seq experiment and related bioinformatics data analysis. Cancer genomics and the TCGA project. Introduction to systems biology and network theory. Examples of networks in biology and genomics. Basic elements of the R statistical programming language. Examples of research articles available on Pubmed discussing topics related to arguments related to the course.
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Course Forensomics and biotechnological applications: Biotechnological applications
Course ID MS1869
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum SYSTEM BIOMEDICINE
Teaching leader ELLIS Steven Robert
Teachers Ellis Steven Robert
CFU 6
Teaching duration (hours) 36
Individual study time 114
SSD BIO/13 - BIOLOGIA APPLICATA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Grading type V
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Course Forensomics and biotechnological applications: Forensomics
Course ID MS1870
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum SYSTEM BIOMEDICINE
Teaching leader GINO Sarah
Teachers Gino Sarah
CFU 5
Teaching duration (hours) 25
Individual study time 100
SSD MED/43 - MEDICINA LEGALE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Students must know the general principles of omics technologies applied to forensic sciences.
Contenuti The course aims to give students useful elements to understand the use of omics in forensic sciences. At the same time, the teaching also deals with medico-legal, legal and ethical issues such as informed consent, professional and industrial secrecy, the role of the ethics committee in Italian, European, and extra-European context.
Testi di riferimento No specific text recommended. Material provided by the professor
Obiettivi formativi The course provides useful elements for the application of genomics, metabolomics, lipidomics, proteomics and transcriptomics in forensic sciences. It is also proposed to provide students with legal, medico-legal and bioethical elements useful for research and professional activities.
Prerequisiti Fundamentals of cellular and molecular biology, and genetic
Metodi didattici Frontal lesson
Modalità di verifica dell'apprendimento Written exam (close and / or open questions)
Programma esteso Informed consent Professional and industrial secrecy - Privacy The role of the ethics committee Forensic genomics: Crime Scene Investigation, characterization of evidence, forensic genetics (human identification, ancestry, phenotyping, DNA methylation) Microbiome and forensic sciences: human identification, estimation of PMI, causes of death, prediction of chronological age Forensic metabolomics and lipidomics: instruments and estimation of PMI Forensic proteomics: instruments and estimation of PMI, prediction of chronological age, human identification Forensic transcriptomics: RNA and body fluids identification, RNA and estimation of PMI
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Course Functional Genomics
Course ID MS0455
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GIORDANO Mara
Teachers Giordano Mara, Baldanzi Gianluca, Gagliardi Mara
CFU 11
SSD MED/03 - GENETICA MEDICA, BIO/10 - BIOCHIMICA, BIO/18 - GENETICA
Course type Attività formativa integrata
Course mandatoriety OBB
Year 1
Period Primo Semestre
Grading type V
Lingua insegnamento ENGLISH
Modules
Course ID Course SSD Teachers Agenda web
MS0456Genomic analysis MED/03 - GENETICA MEDICA Giordano Mara
MS0457Regulation of gene expression BIO/10 - BIOCHIMICA Baldanzi Gianluca
MS0458Genetics BIO/18 - GENETICA Gagliardi Mara
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Course Functional Genomics: Genomic analysis
Course ID MS0456
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GIORDANO Mara
Teachers Giordano Mara
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD MED/03 - GENETICA MEDICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Modalità di verifica dell'apprendimento The final exam for the comprehensive course will consist in a written exam including all the three subjects of the course, namely Genomic Analysis, Regulation of gene expression and Genetics. For each subjects there will be four statements requiring the answer true/false and eventually a brief comment of maximum 10 lines. The maximum time allowed will be 90 minutes. The results will be given within three-four days and if the student wishes to increase the grade he/she can do an oral exam.
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Course Functional Genomics: Regulation of gene expression
Course ID MS0457
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader BALDANZI GIANLUCA
Teachers Baldanzi Gianluca
CFU 5
Teaching duration (hours) 20
Individual study time 95
SSD BIO/10 - BIOCHIMICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento ENGLISH
Risultati di apprendimento attesi Understanding the mechanisms of regulation of gene expression and how such knowledge can be used in research or therapy.
Contenuti After a quick review of gene structure in eukaryotes and prokaryotes, the issue of gene expression regulation will be presented. The first part of the course will deal with prokaryotes gene expression and its regulation. A series of examples will illustrate the possibility of engineering promoters and genes for biotechnological purposes. The second part of the course will deal with the eukaryotes gene expression and the multiple levels of its regulation. Issues: 1) chromatin organization and structure, 2) transcription factor function, 3) pre-initiation complex, 4) transcription termination, 5) RNA stability and 6) RNA translation. The third part of the course will deal with the mechanisms by which extracellular signals and intracellular cues regulates gene expression in eukaryotes. A particular emphasis will be placed on the alterations of gene expression in cancer and on the regulation of gene expression in function of nutrients and metabolic status of the cell. Basic biochemical techiques
Testi di riferimento For the improvement of students background Harper's Illustrated Biochemistry, 29e, Robert K. Murray, David A. Bender, Kathleen M. Botham, Peter J. Kennelly, Victor W. Rodwell, P. Anthony Weil Please note: available on Access Medicine for our students Molecular Biology of the Cell - Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter Edition:5th Please note: the 4th edition is available for free on NCBI bookshelf http://www.ncbi.nlm.nih.gov/books/NBK21054/ Suggested textbook Genomes 3 By Terence A. Brown Please note: the 2nd edition is available for free on NCBI bookshelf http://www.ncbi.nlm.nih.gov/books/NBK21128/ Cell Signalling Biology Professor Sir Michael Berridge http://www.biochemj.org/csb/ An extended series of reviews evidenced during the course.
Obiettivi formativi Strengthen the knowledge and operational skills in molecular biology applied to the study of gene expression. Provide the operational bases in the biochemical laboratory.
Prerequisiti Basic Biology: Prokaryotes and Eukaryotes intracellular organization. Basic biochemistry: DNA and RNA structure and metabolism, protein synthesis, enzyme proprieties, major metabolic pathways. Basic molecular biology: concept of gene, gene expression, promoter, transcription factor, RNA synthesis and degradation. For the students lacking a strong background in life sciences a series of readings will be proposed to improve their skills on those topics.
Metodi didattici During the course, slides and webinars will be extensively used. Classwork will be complemented by an introduction in the laboratory. Students will also undertake teacher driven research/presentation/teamwork. A copy of the slides and the websites used during the course will be available on the DIR site.
Altre informazioni -
Modalità di verifica dell'apprendimento The final exam for the comprehensive course will consist in a written exam including all the three subjects of the course, namely Genomic Analysis, Regulation of gene expression and Genetics. For each subjects there will be four statements requiring the answer true/false and eventually a brief comment of maximum 10 lines. The maximum time allowed will be 90 minutes. The results will be given within three-four days and if the student wishes to increase the grade he/she can do an oral exam.
Programma esteso Topic 1: Gene expression regulation Recall of the organization of genes in eukaryotes (enhancers, promoters, transcription start site, introns/exons, transcription termination sites). Control of gene transcription at the level of transcription initiation rate, splicing and alternative splicing, mRNA stability, RNA interference and microRNA. Transcription factors organization: structure of DNA binding and trans-activating domains. Major DNA regulatory sequences. Assembly of RNApolII transcription initiation complex. Methods to study DNA regulatory elements, Promoter structure and transcription factor binding sites. Transcription factors and chromatin structure, chromatin remodeling and gene expression. Post transcriptional modification of histones and the histone code. DNA mutilation. Other ways to regulate gene expression: alternative promoters, alternative splicing, control of mRNA stability, control of mRNA translation, miRNA and RNA interference. Topic 2: Control of gene expression by extracellular and intracellular cues. How signal transduction pathways regulates gene expression: The nuclear receptor family The STAT family ERK signaling to the serum response elements. Myc in growth factors signaling and tumor formation Hif in the response to hypoxia and tumorigenesis P53 mutations, cancer and metabolism Topic 3: Tumor cell metabolism and the way to study it. Peculiarities of tumor metabolism, the Warburg effect and beyond. Methods to study metabolism, integrating gene expression, metabolomics and in vivo data. Use of mass spectrometry, PET/NMR probes, and electrodes (Oxygen Consumption Rate – ExtraCellular Acidification Rate) to study metabolism in living cells/tissues/patients. Experimental manipulation of metabolism and possible routes for cancer therapy. Topic 4: How oncogenes and altered signaling pathways regulates tumor cell metabolism. Tumor metabolomics, onco-metabolites and the peculiarities of tumor metabolism. How alterations of signal transduction machinery and gene expression integrates with a peculiar micro-environment to dictate tumor metabolism. How alterations of the metabolism contributes to tumor development (proliferation, migration, resistance to cell death).
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Course Functional Genomics: Genetics
Course ID MS0458
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader GAGLIARDI Mara
Teachers Gagliardi Mara
CFU 1
Teaching duration (hours) 8
Individual study time 17
SSD BIO/18 - GENETICA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category C
Year 1
Period Primo Semestre
Frequenza obbligatoria 1
Grading type V
Lingua insegnamento ENGLISH
Testi di riferimento No textbook. Students will study on the scientific papers discussed during the classes and made available from the professor.
Obiettivi formativi The aim of this course is to learn most recent data on non-coding elements in the genome and in mammalian transcriptome. Specifically, the course will focus on long non-coding RNAs (lncRNAs) their structure and functions. Moreover, the students will know about antisense transcription, its main features and its function in the cell. Examples will be provided on possible therapeutic applications of lncRNAs. An additional aim of the course is the discussion of a recent published paper focusing on recently discovered lncRNA and its function. Experimental details will be discussed.
Prerequisiti The students must have a solid background in Molecular Biology, Genetics and Cell Biology
Metodi didattici The students receive copy of PDF files of scientific papers discussed in the course.
Altre informazioni nputs from the professor on bibliographic search in the field of lncRNAs.
Modalità di verifica dell'apprendimento The final exam for the comprehensive course will consist in a written exam including all the three subjects of the course, namely Genomic Analysis, Regulation of gene expression and Genetics. For each subjects there will be four statements requiring the answer true/false and eventually a brief comment of maximum 10 lines. The maximum time allowed will be 90 minutes. The results will be given within three-four days and if the student wishes to increase the grade he/she can do an oral exam.
Programma esteso Complexity of the human genome and role of non-coding elements in contributing to genome complexity Definition of mammalian transcriptome and history of the discovery of lncRNAs Features of lncRNAs in comparison with those of protein-coding mRNAs Description of the experimental procedures used to discover lncRNA features Antisense transcription Description of lncRNA functions Therapeutic applications of lncRNAs: present and future prospects Seminar-type discussion on a newly discovered lncRNA: features and function (experimental details will also be discussed)
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Course Regenerative Medicine: Molecular Biology
Course ID MS0492
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader PELICCI Giuliana
Teachers Pelicci Giuliana
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD BIO/11 - BIOLOGIA MOLECOLARE
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi Provide basic knowledge of stem cell biology, reprogramming and pluripotency with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.
Contenuti Review of general topics of Molecular Biology relevant to the understanding of the course. Chromatin Remodeling: Histone Modifications, DNA methylation Structural classes of transcription factors (Myc, Fos) Epigenetic definition: epigenetic diseases and epigenetic therapies. Oncogenes and tumor suppressor genes. P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function. Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions. Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells. Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep). Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency. Parallelisms between epigenetic reprogramming and cancer. Therapeutic use of iPS Cells and embryonic stem cells.
Testi di riferimento Molecular Biology of the Cell Alberts Scientific Papers
Obiettivi formativi Provide basic knowledge of stem cell biology, reprogramming and pluripotency with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.
Prerequisiti A basic understanding of cell biology and molecular biology.
Metodi didattici PowerPoint presentations of lessons and articles provided by the lecturer
Modalità di verifica dell'apprendimento Written Examination and oral presentation of a scientific journal article.
Programma esteso Review of general topics of Molecular Biology relevant to the understanding of the course. Chromatin Remodeling: Histone Modifications, DNA methylation Structural classes of transcription factors (Myc, Fos) Epigenetic definition: epigenetic diseases and epigenetic therapies. Oncogenes and tumor suppressor genes. P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function. Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions. Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells. Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep). Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency. Parallelisms between epigenetic reprogramming and cancer. Therapeutic use of iPS Cells and embryonic stem cells.
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Course Regenerative Medicine: Histology, Gene and Cell Therapy
Course ID MS0479
Academic Year 2020/2021
Year of rule 2020/2021
Degree MEDICAL BIOTECHNOLOGY
Curriculum CORSO GENERICO
Teaching leader FOLLENZI Antonia
Teachers Follenzi Antonia
CFU 5
Teaching duration (hours) 30
Individual study time 95
SSD BIO/17 - ISTOLOGIA
Course type Modulo di sola Frequenza
Course mandatoriety OBB
Course category B
Year 1
Period Secondo Semestre
Grading type V
Lingua insegnamento English
Risultati di apprendimento attesi At the end of the course the student should be able to read an article of gene and cell therapy and discuss on it with scientists of the field
Testi di riferimento For italians: Lollini, Terapia genica, ZANICHELLI Animated gene therapy course, Utah University, USA Journals: Human Gene Therapy Gene Therapy Cancer Gene Therapy Journal of Gene Medicine Molecular Therapy Nature Science Databases: NCBI - MEDLINE, GenBank, Genomi, ecc. Genetic Modification Clinical Research Information System (GeMCRIS) Clinicaltrials USA Wiley clinical trial database
Obiettivi formativi The students should be come able to discuss cell and gene therapy published articles and fundamental vectorology used in gene therapy in preclinical studies and clinical trials.
Prerequisiti basic information of Molecular and cellular biology
Metodi didattici Slides and PDF articles
Altre informazioni Group seminar prepared by the students on a chosen topic.
Modalità di verifica dell'apprendimento Commentary on a cell and gene therapy paper and an oral discussion on topics of the course program.
Programma esteso Stem cells, cellular reprogramming and use of these cells in cell therapy protocols in preclinical and potential applications in the clinic. Isolation, in vitro expansion and preparation of cells for experimental cell therapy. Vectors used for gene transfer: viral vectors and non-viral. Origin, construction and modification. Modulation of Expression cassettes: Constitutive and regulated expression. transcriptional or post-transcriptional expression regulation Immune response to vectors and transgenes used in gene transfer. Concepts of ex vivo and in vivo gene therapy. Clinical trials using cell and gene therapy strategies of target diseases. use of anilmals to model genetic diseases
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Year Course ID Course Teachers SSD Curriculum Site CFU
1 MS1864 Big Data Analysis Cora' Davide BIO/11 System Biomedicine 5
1 MS0452 Fertility, reproduction and endocrine system biotechnologies Marzullo Paolo, Surico Daniela MED/13, MED/40 The Molecular Basis Of Disease 4
1 MS0455 Functional Genomics Giordano Mara, Baldanzi Gianluca, Gagliardi Mara MED/03, BIO/10, BIO/18 All 11
1 MS0459 Internal Medicine Bellan Mattia MED/09 The Molecular Basis Of Disease 5
1 MS0465 Molecular therapy Ellis Steven Robert, Colangelo Donato BIO/13, BIO/14 The Molecular Basis Of Disease 11
1 MS1881 Molecular virology Gariglio Marisa MED/07 System Biomedicine 6
1 MS0469 Neoplastic and proliferative disorders Gaidano Gianluca, Isidoro Ciro MED/15, MED/04 The Molecular Basis Of Disease 12
2 MS0461 Molecular diagnostics Boldorini Renzo Luciano, Capello Daniela, Rolla Roberta MED/08, BIO/12, MED/05 All 11
2 MS2001 Scientific English Course for the thesis Kalinina Irina NN All NOVARA 0
2 MS0480 Transplantation and Biocompatibility Patriarca Andrea, Cantaluppi Vincenzo, Chiocchetti Annalisa, Boccafoschi Francesca, Rimondini Lia, Mantovani Diego MED/15, MED/14, MED/04, BIO/16, MED/28, BIO/11 All 7
Data synched: 16/05/2021, 01:10