Ateneo - UPO

Citogenetics Hystory Cytogenetics techniques and applications Karyotyping and Chromosomal banding Normal and aberrant karyotypepostnatal and prenatal cytogeneticscytogenetics in tumorsMolecular cytogenetics: Fluorescent in situ Hybridization (FISH) and molecular karyotyping

In coda alle dispense fornite agli allievi

Students will be able to know principles and applications of cytogenetics procedures and analysis. Students must know limits and advantages of each teqnique.

Students must have basic genetics and molecular biology knowledge

Frontal lessons
Interactive activitiesLaboratory activities

Multiple choice test and essey questions

Cytogenetics History Hypotonic solution and colchicine
Mitosis and meiosis
Banding teqniques and resolution
Karyotype analysis, advantages and limits
Normal and disease Karyotype
Chromosomal aberrations: atypical number of chromosomes or a structural abnormality
Prenatal chromosomal and postnatal Molecular cytogenetics; Fluorescent in situ hybridization (FISH), advantages and disadvantages
The choice of probes. Probe labelling Chromosome staining dyes
Molecular karyotyping : principles, resolution, limits and advantages
Copy number variations (CNV) : common polymorphisms versus pathological CNVCNV databases
Guidelines for Cytogenetics analysis:
Cytogenetics laboratory equipment
and resolution
Karyotype analysis, advantages and limitsNormal and disease KaryotypeChromosomal aberrations:  atypical number of chromosomes or a structural abnormality
Prenatal chromosomal and postnatal Molecular cytogenetics; Fluorescent in situ hybridization (FISH), advantages and disadvantages
The choice of probes. Probe labelling Chromosome staining dyes
Molecular karyotyping : prinicples, resolution, limits and advantages
Copy number variations (CNV) : common polymorphisms versus pathological CNV
CNVs databases
Guidelines for Cytogenetics analysis:
Cytogenetics laboratory equipment

Students should be able to know limits and advantages of each cytogenetic procedures and their applications