Ateneo - UPO

Gas Chromatography Mass Spectrometry. Gas-chromatography-mass spectrometry (GC-MS). Liquid chromatography-mass spectrometry (LC-MS) Interfacing problems Pollutants and related methods of analysis Pesticides. Dibenzodioxins and dibenzofurans. Polychlorinated biphenyls (PCB). POP list

Sckoog and Leary “Chimica analitica strumentale” Edises D.C.Harris “Chimica analitica quantitativa” 2’ ed. Italiana .Zanichelli Watson, “Analisi farmaceutica”, 3th edizione, Edra C. Baird “ Chimica ambientale” ed. Zanichelli (2006) Watson and Sparkma “Introduction to Mass Spectrometry” ed.Wiley

Knowledge of advanced analytical techniques applicable to the determination of drugs, pollutants and bioactive substances present in complex matrices

Basic knowledge of organic chemistry and pharmaceutical analysis

Lessons

Students with physical disabilities, Learning Disabilities or Special Education Needs can request specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/services-students-physical-or-learning-disabilities

Students with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects.

Assessment of learning will be carried out through an oral exam. During the oral exam, the student's ability to read and interpret a scientific article concerning the analytical techniques presented during the course and their application will be assessed. The knowledge of the basic principles of the same will also be evaluated. The student will receive the scientific article to comment on at the closing of the chosen appeal; they will have to present it in a maximum of 10 minutes using any tool deemed useful by them. To obtain the final mark of the course, the student must demonstrate that they have understood and are able to use the fundamental concepts of each topic. The evaluation, expressed in thirtieths, will be based on the following criteria: 1) knowledge and ability to understand the theoretical and applied aspects of the topics studied; 2) ability to connect the topics studied; 3) communication skills and appropriateness in the use of technical language.

Principles of gas chromatography. Gas Chromatography: injectors, columns, detectors. Selectivity, efficiency, resolution. Van Deemter equation. Retention indices: Kovats indices Headspace methods: static, dynamic, SPME Principles of mass spectrometry. Sources: IE, CI, ESI, APCI, Maldi. Analyzers: magnetic, double focusing, quadrupole, ion trap, TOF. cyclotronic resonance, Orbitrap. Resolving power and resolution. Tandem mass spectrometry. High resolution mass spectrometry. Mass spectrum. Molecular ion and pseudomolecolar ion, isotopic abundance, fragmentations. Gas-chromatography-mass spectrometry (GC-MS). Definition of the gas chromatographic method. Definition of the spectrometric method. Full scan, SIM, MS / MS. Qualitative analysis. Use of database. Quantitative analysis. Calibration curve. Internal standard method. Isootopic dilution Liquid chromatography-mass spectrometry (LC-MS) Interfacing problems Qualitative and quantitative analysis. Definition of the chromatographic method. Definition of the spectrometric method Pollutants and related methods of analysis Pesticides. Chlorinated, organophosphorus, carbamates insecticides. Pyrethrins and pyrethroids insecticides. Erbicides: a) interfering with the growth factors of the plant: auxinic herbicides; b) interfering with the photosynthesis process: triazine, cloroacetamidi, urea derivatives, dipiridilici derivatives; c) interfering the synthesis of aromatic amino acids: glyphosate; d) interfering the cell division: sulfonylureas. Inorganic and metal organic fungicides, organic fungicides: dithiocarbamates, benzimidazoles, and diphenyl. Repellents. Dibenzodioxins and dibenzofurans: structures, sources of pollution, toxicity. Polychlorinated biphenyls (PCB): structures, sources of pollution, toxicity, methods of disposal POP list

Knowledge of advanced analytical techniques of chromatography and mass spectrometry . Knowledge of principal class of polluting substances. Ability to apply such knowledge and skills in the determination of bioactive compounds in complex matrices.