Ateneo - UPO

The course of STRUCTURAL BIOLOGY is delivered to students of the 3th and 4th of both PHARMACY that of CTF as credit free. The course aims to give the student the knowledge of the fundamental principles of bio-crystallography with emphasis on the potential and limitations.
• Structural classification and analysis of protein structures.
• Knowledge of the structure-function relationships in the series of protein treated in the course.
• Critical analysis of a protein structure determined by X-ray crystallography
• Analysis structures capabilities and extraction of structural information

• Gregory A. Petsko & Dagmar Ringe. “PROTEIN STRUCTURE AND FUNCTION” OUP Oxford, 2008 ISBN 9780199556847
• Andreas Liljas, lars Liljas, jure Piskur, Goran lindblom, Poul Nissen & Morten Kjeldgaard. “STRUCTURAL BIOLOGY” World Scientific. ISBN: 9788808182173

The course aims to provide knowledge on bio-crystallography basic principles, with emphasis on the potentials and limitations. It will also give at the student fundamental knowledge on the classification at the structural level of same enzymes and the analysis of protein structures. Knowledge of the structure-function relationships in the series of protein treated in the course. Capacity for critical analysis of a specific X-ray 3D structure to extract information about protein activity. Also it will be encouraged the research of most recent data published in scientific journals of international importance to deepen all the studied arguments using the scientific web-search "PubMed".

None.
However, it will be required a good preparation in biochemistry.

ex-cathedra traditional lectures

It is possible to view lecture slides and supplementary materials on the University's Networked Learning website: https://www.dir.uniupo.it/
Students with disabilities or Specific Learning Disorders (SLD) or Special Educational Needs (BES) can request services and tools
specifically dedicated to them by contacting the Staff Development and Coordination Careers and Student Services and consulting the dedicated page of the University website:https://uniupo.it/it/servizi/servizi-studenti-disabili-e-dsa Students with disabilities, DSA, BES, once they have made contact with the University Staff, they can contact the teacher in charge of the teaching in relation to the declination of the examination modalities, regarding the didactic aspects.

Oral exams.
The oral exam will be assessed the student's ability to read and interpret a scientific article about the crystallographic structure of a protein. It will also assess knowledge of the basic principles of protein crystallography.

Crystallization Techniques of Biological Macromolecules.
• X-ray diffraction (The Synchrotron).
• How do you solve a three-dimensional structure of a biological macromolecule.
• Databases: the PDB format file.
• Visualization and analysis of three-dimensional structures through computer graphics
• FOLDING-From the sequence to the three-dimensional structure.
Small monographic courses (3 hours by topic):
• Biological context and structure-function relationships:
• ßMiosina / Actin
• ATP-synthase
• RNA-polymerase
• Folding related diseases
• Virus -Interaction structure-function anti-HIV drugs

At the end of the course, the students will be able to read in a critical way, scientific literature regarding the relationsheep between structure-function of a protein, in particularly of enzymes. In addition, the students will be able to analyze specific enzymes interactions with their ligands, by displaying them on specific programs of graphics.