Ateneo - UPO

theoretical principles and practical aspects of main experimental models used in preclinical research

Material provided by the teacher

Provide the theoretical basis of the main methods used for the generation and use of experimental models in vitro, ex vivo and in vivo, used in preclinical research

The student must know cellular biology and basic genetics and, in particular, the molecular and cellular mechanisms that regulate replication, cell growth and the gene expression program in both prokaryotic and eukaryotic organisms.
The student must also know molecular biology, histology and the main techniques related to recombinant DNA technologies

Classroom lessons.
Students with physical disabilities, Learning Disabilities or Special Education Needs can request specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse
e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/services- students-physical-or-learning-disabilities.
Students with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects.

Supplementary materials will be available on DIR website

Written test

Introduction to experimental models used in preclinical studies.
Cell cultures: general concepts
Primary cells
Stem cells
Cancer cells
Manipulation, engineering, analysis,
immortalization, banking
Static and dynamic cocultures
Three-dimensional (3D) cell cultures
Spheroids
Scaffold
Organoids
Organ on chip
Platforms to model disease pathogenesis
Platforms to mimic human physiology
Animal models
Mouse models for the study of
human pathologies

The student will become familiar with the main methods and technologies used to study and analyze human pathologies using experimental models. Acquired familiarity with instrumentation and working methods of a research / analysis laboratory