Ateneo - UPO

Gas Chromatography
Mass Spectrometry.
Gas-chromatography-mass spectrometry (GC-MS).
Liquid chromatography-mass spectrometry (LC-MS) Interfacing problems
Pollutants and related methods of analysis
Pesticides.
Dibenzodioxins and dibenzofurans.
Polychlorinated biphenyls (PCB).
POP list

D.C.Harris “Quantitative chemical analysis” ed.Zanichelli
Watson, “Pharmaceutical analysis”, 3th ed. Elsevier
C. Baird “ Enviromental chemistry” Freeman and Company New York
Watson and Sparkma “Introduction to Mass Spectrometry” ed.Wiley

Knowledge of advanced analytical techniques applicable to the determination of drugs, pollutants and bioactive substances present in complex matrices

Basic knowledge of organic chemistry and pharmaceutical analysis

Lessons

The exam is an oral interview on the topics of the program.The evaluation, expressed out of thirty, will be carried out on the basis of the following criteria:
1)knowledge and understanding of the topics studied;
2) ability to connect between the topics studied;
3)communication skills and appropriateness in the use of technical language;

Principles of gas chromatography.
Gas Chromatography: injectors, columns, detectors.
Selectivity, efficiency, resolution. Van Deemter equation.
Retention indices: Kovats indices
Headspace methods: static, dynamic, SPME
Principles of mass spectrometry.
Sources: IE, CI, ESI, APCI, Maldi.
Analyzers: magnetic, double focusing, quadrupole, ion trap, TOF. cyclotronic resonance, Orbitrap.
Resolving power and resolution.
Tandem mass spectrometry.
High resolution mass spectrometry.
Mass spectrum. Molecular ion and pseudomolecolar ion, isotopic abundance, fragmentations.
Gas-chromatography-mass spectrometry (GC-MS).
Definition of the gas chromatographic method. Definition of the spectrometric method. Full scan, SIM, MS / MS.
Qualitative analysis. Use of database. Quantitative analysis. Calibration curve. Internal standard method. Isootopic dilution
Liquid chromatography-mass spectrometry (LC-MS) Interfacing problems
Qualitative and quantitative analysis.
Definition of the chromatographic method. Definition of the spectrometric method
Pollutants and related methods of analysis Pesticides. Chlorinated, organophosphorus, carbamates insecticides. Pyrethrins and pyrethroids insecticides. Erbicides: a) interfering with the growth factors of the plant: auxinic herbicides; b) interfering with the photosynthesis process: triazine, cloroacetamidi, urea derivatives, dipiridilici derivatives; c) interfering the synthesis of aromatic amino acids: glyphosate; d) interfering the cell division: sulfonylureas. Inorganic and metal organic fungicides, organic fungicides: dithiocarbamates, benzimidazoles, and diphenyl. Repellents.
Dibenzodioxins and dibenzofurans: structures, sources of pollution, toxicity.
Polychlorinated biphenyls (PCB): structures, sources of pollution, toxicity, methods of disposal
POP list

Knowledge of advanced analytical techniques of chromatography and mass spectrometry . Knowledge of principal class of polluting substances. Ability to apply such knowledge and skills in the determination of bioactive compounds in complex matrices.