Ateneo - UPO

Microbiological diagnosis: principles and purposes
Direct diagnosis
1. Cultural isolation (colours, soils, sowing, cultivation conditions)
2. Microscopic (bright field, dark field, phase contrast and fluorescence)
Presumptive and final identification:
Direct diagnosis
a) Biochemistry,
b) Serological
c) Molecular
Antibiogram
Indirect diagnosis
1. Complement fixation reaction
2. Agglutination reaction
3. Enzyme immunoassay (ELISA)
4. Immunofluorescence (IF) assay
5. Radioimmunoassay (RIA)
The Microbiota

Material provided by the teacher (ppt, links to official websites; updated documents; scientific publications)

Knowledge of methodologies and technologies at the service of microbiological diagnostics

Knowledge of the principles of microbiology, including bacteria, viruses and their pathogenicity mechanisms.

Interactive lessons; use of interactive platforms (Kahoot) to probe previous knowledge and stimulate active participation.
Power point lessons; Group work

for further information contact barbara.azzimonti@uniupo.it

Multiple choice test and group work

Microbiological diagnosis: principles and purposes
Direct diagnosis
1. Cultural isolation (colours, soils, sowing, cultivation conditions)
2. Microscopic (bright field, dark field, phase contrast and fluorescence)
Presumptive and final identification:
Direct diagnosis
a) Biochemistry,
b) Serological
c) Molecular
Antibiogram
Indirect diagnosis
1. Complement fixation reaction
2. Agglutination reaction
3. Enzyme immunoassay (ELISA)
4. Immunofluorescence (IF) assay
5. Radioimmunoassay (RIA)
The Microbiota

Understand the objectives of microbiological diagnosis, distinguishing between direct and indirect diagnosis.
Know the importance of microbiological diagnosis to identify pathogens and guide therapy
Analyze the role of microbiological diagnosis in epidemiological surveillance and infection control.