Ateneo - UPO

The cell culture laboratory: characteristics and peculiarities
- Sterility techniques: material and equipment.
- Observation of cells under an inverted microscope
- General principles on in vitro cell cultivation: media, sera, antibiotics
- Cultivation of stabilized cell lines in adhesion and in suspension
- Cell freezing for storage in liquid nitrogen and thawing
- Precautions to prevent crop pollution and possible decontamination.
- cells banks

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Risultati della traduzione
Risultato di traduzione
www.farm.unisi.it/assays and dosages-cell cultures pdf.
www.gginofarm.altervistaorg.
www.online scuola zanichelli.it

The student must acquire the main theoretical and practical notions on eukaryotic cell cultures.

Must know the basic activities for culturing, growing, expanding cell cultures starting from cells, tissues and organs
He must know all the technical maneuvers to work in sterility, recognize any pollution, and know how to eliminate it.
Must know the difference between primary cultures and cell lines

not provided

Multiple choice written test

The cell culture laboratory: characteristics and peculiarities.
- Sterility techniques: material and instruments.
- the observation of the cells with the inverted m.o.
-General principles of in vitro cell cultivation: soils, serums, antibiotics.
-Cultivation of stabilized cell lines in adhesion and in suspension.
-Cellular freezing for storage and thawing.
Precautions to prevent crop pollution and possible decontamination.
-cells banks.