Ateneo - UPO

theoretical principles and some practical aspects of the main recombinant DNA techniques

- Jeremy Dale, et al. "Dai Geni ai Genomi" - EDISES (terza edizione)
- James D. Watson et al. "DNA Ricombinante" - Zanichelli (seconda edizione)
- Terry A. Brown "Biotecnologie Molecolari" - Zanichelli (seconde edizione)

Provide the theoretical and practical bases of the methods and technologies used in the study and analysis of gene expression, with particular emphasis on recombinant DNA techniques used to clone, express and analyze gene products of biomedical interest and applications. Importantly, the frequency of a dedicated laboratory is mandatory.

The student must know both cellular biology and basic genetics, and in particular the molecular and cellular mechanisms regulating replication, cell growth and gene expression programs in both prokaryotic and eukaryotic organisms.

classroom and laboratory lessons

available on DIR website

written test (multiple-choice questions)

Cloning. Gene manipulation strategies. Key features of cloning vectors. Expression of recombinant proteins. Strategies to generate and maintain genetically modified organisms. Technologies used in genetic diagnosis. Cloning and analysis of genotypes. PCR: Principles and Uses. Targeted mutagenicity. Expression of recombinant proteins in E. coli. Methods of gene transformation. Expression of recombinant proteins in eukaryotic cells. Expression of transgenes in animal models. DNA arrays, Gene-chips. PTT, SNP. Proteomics. Genetic strategies to manipulate the phenotype of organisms of socio-economic interest. Animal & Plant Pharming.

The student will become familiar with the main methods and technologies used to study and analyze gene expression, together with the main techniques used to clone, express and analyze gene products of biomedical interest and application. Acquired familiarity with instrumentation and working methods of a research / analysis laboratory.