Ateneo - UPO

The course illustrates the main DNA analysis techniques applied to human genetics. It explains the principles behind DNA analysis techniques and how they are applied to analyze human genomic DNA.
Use of the main genomic analysis software and for reading sequences

no text, only the slides provided by the teacher

to Understand the main DNA analysis techniques applied to human genetics

basic knowledge of human genetics

lectures followed by experience in the laboratory for putting into practice the techniques learned.
This is followed by a lesson on the main genomic analysis software using the computer room by applying the analysis of the results obtained in the laboratory

written exam with multiple choice quiz

Principles on which DNA analysis techniques are based.
Hybridization between nucleic acids
Concept of stringency and factors that modify it
melting temperature and factors affecting Tm
Absorbance law of lambert beer
nucleic acid extraction techniques
quantitative and qualitative analysis of the extracted DNA
agarose gel electrophoresis
Restriction enzymes and cutoff frequency
Polymerase chain reaction, principle
Design of oligonucleotides and criteria to be used
Use of the BLAst bioinformatics program for oligonucleotide specificity analysis
Internal PCR Controls: Negative Control, Positive Control
Electrophoresis on agarose gel and capillary electrophoresis for analysis of the amplified product
Electrophoretic analysis of amplifiers containing repeated sequences or after cutting with restriction enzymes.
purification of amplification products: techniques
Sanger sequencing, principle
Sanger sequencing procedure and analysis of electropherograms after capillary electrophoresis
examples of electropherogram analysis

Understanding of the main DNA analysis techniques and their field of application in human genetics.
knowledge of the limits and advantages of the techniques illustrated