Ateneo - UPO

Review of general topics of Molecular Biology relevant to the understanding of the course.
Chromatin Remodeling: Histone Modifications, DNA methylation
Structural classes of transcription factors (Myc, Fos)
Epigenetic definition: epigenetic diseases and epigenetic therapies.
Oncogenes and tumor suppressor genes.
P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function.
Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions.
Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells.
Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep).
Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency.
Parallelisms between epigenetic reprogramming and cancer.
Therapeutic use of iPS Cells and embryonic stem cells.

Molecular Biology of the Cell Alberts
Scientific Papers

Provide basic knowledge of stem cell biology, reprogramming and pluripotency with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.

A basic understanding of cell biology and molecular biology.

Lessons in the classroom supported by the projection of films aimed at
helping the learning process. Development of classroom problems
involving the use of concepts and tools illustrated during lectures.
Practise.
To help students in their study and preparation for the final exam,
deliveries will be assigned during the course which will contribute to a
minimal part of the final grade.

Test of ongoing learning, through interactive tests in the classroom using the wooclap platform.

Written exam and evaluation of assignments (preparation of scientific posters and presentations) concerning scientific articles assigned to students during the course.

Review of general topics of Molecular Biology relevant to the understanding of the course.
Chromatin Remodeling: Histone Modifications, DNA methylation
Structural classes of transcription factors (Myc, Fos)
Epigenetic definition: epigenetic diseases and epigenetic therapies.
Oncogenes and tumor suppressor genes.
P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function.
Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions.
Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells.
Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep).
Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency.
Parallelisms between epigenetic reprogramming and cancer.
Therapeutic use of iPS Cells and embryonic stem cells.

Provide basic knowledge of stem cell biology, reprogramming and pluripotency with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.