Ateneo - UPO

Molecular diagnostics: applications, the concept of molecular biomarkers, and molecular diagnostic techniques in clinical practice.

Diagnsotica molecolare nella medicina di laboratorio- Piccin editore

Knowledge of biomolecular tools in disease diagnosis and prevention

Knowledge of molecular biology

Lectures with PowerPoint presentations

Students with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects.

exam written with mutiple choices

Course Introduction. Review of the fundamentals of molecular biology. Eukaryotic cell: structure and functions. DNA, RNA, and proteins. Point mutations and gene rearrangements (chromosomal mutations). Examples of prenatal diagnosis (karyotype analysis).
Definition and classification of molecular biomarkers: diagnostic, prognostic, and predictive with examples. Stages of molecular diagnostics. Introduction to omics sciences. Overview of the role of artificial intelligence in diagnostics (machine learning).
Liquid biopsy as a source of biomarkers. Comparison between liquid biopsy and tissue biopsy. Applications and advantages of molecular diagnostics.
Diagnostic techniques. Practical exercises: PCR for mycoplasma identification. Flow cytometry: isolation of peripheral blood mononuclear cells and identification of T lymphocytes and B lymphocytes.

At the end of the course, the student will be able to:
- Present the course topics clearly and comprehensibly, using the correct scientific terminology.
- Deepen their knowledge of advanced diagnostic technologies.