Ateneo - UPO

Description of the principal chromatographic and spectroscopic techniques.

Lesson materials, Chimica Analitica Strumentale - EDISES

Provide the basics on the operation and use of the most modern instrumental analytical techniques. To enable students to follow simple analytical methods in the laboratory and provide them with the fundamental concepts for good laboratory practice.

None

In class lessons, laboratory experiences

The knowledge is evaluated by classroom exercises.

instrumental analytical chemistry. Oral exam with three open questions on the main methods of instrumental analysis covered in the course.
laboratory. Evaluation of the relationship of each experience.

Introduction to chromatographic techniques: theory of theoretical plates, velocity theory, Van Deemter equation, parameters for the evaluation of the goodness of chromatographic separation: efficiency, resolution, scodaturation, width and asymmetry of peaks. Chromatographic separation mechanisms: adsorption, distribution, ion exchange, dimensional exclusion. Liquid chromatography and gas chromatography. HPLC instrumentation: pumps, sample introduction systems, columns, detectors. GC instrumentation: sample introduction systems, columns, detectors.
Introduction to spectroscopic techniques of atomic absorption and emission. Atomic flame absorption, graphite oven, inductively coupled plasma. Instrumentation used: hollow cathode lamp and discharge lamp without electrodes, atomization system, sample introduction, monochromators, detectors. Spectral interference, chemical, matrix effect. UV-vis spectroscopy: Lambert-Beer law and its deviations, instrumental components

ANALYTICAL CHEMISTRY
Knowledge and understanding
- knowledge of the theoretical/practical bases of the main analytical techniques (chromatographic, elementary, based on mass spectrometry)
- knowledge of the performance indices of analytical methods
- knowledge of unitary laboratory operations and the use of instrumental methods of analysis
Ability to apply knowledge and understanding - to be able to compare different techniques for the same purpose also through analysis of analytical performance indices
- know how to perform analysis
Communication skills
- acquire and know how to use an appropriate chemical vocabulary in relation to the topics covered in the course
- know how to draw up a technical-scientific report
Making judgements
- know how to choose between different analytical techniques to solve a given analytical problem
- know how to deal with a volumetric analysis by making the appropriate choices from an experimental point of view

Learning skills
- ability to use the study material independently to solve analytical problems