Ateneo - UPO

Cell culture techniques of tumor cell lines in monolayer or as multicellular spheroids. Analysis of the cytotoxicity of a drug

Material provided by professor

Acquisition of experimental techniques and methodologies useful for understanding biological processes at a molecular and cellular level.

Bases of cellular and molecular biology

-Lecture
to explain the experimental part.
-Experimental part with entry into the biological laboratory

Mandatory frequency.
Students with physical disabilities, Learning Disabilities or Special Education Needs can request specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/services-students-physical-or-learning-disabilities
Students with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects.

ultiple-choice tests (15 questions, 1 correct answer). The final vote will also take into account the activities carried out in the laboratory and the achievement of the objectives of the course regarding practical skills.

Preparation of cell cultures of tumor cell lines in 2D or as multicellular spheroids in 3D. In vitro evaluation of the cytotoxicity of a drug.
RNA and protein extraction techniques for gene and protein expression analysis.

To demonstrate to know the main two- and three-dimensional cell culture techniques.
At the end of the course, students will be able to evaluate the cytotoxicity of a drug both by using cell counts and by analysis of gene and protein expression.