Ateneo - UPO

Specific methods of treatment of biological, cyto and histological materials meaning of the intraoperative examination. Principles and techniques of nucleic acid extraction, gene amplification methods, techniques and principles of gene sequence analysis.
Principles of classification of solid neoplasms, concepts of staging and grading.
Diagnostics, prognostic and predictive factors of anatomical-pathological interest in the main human neoplastic pathologies: carcinoma of the lung, breast, colorectal, urinary tract, male and female genitals, CNS, skin melanomas, biliary tract neoplasia.
Diagnostics of chronic inflammatory diseases of the gastrointestinal tract such as Hirschsprung disease, chronic gastritis, celiac disease.

Kumar, Abbas, Fausto. Robbins and Cotran Pathologic Basis of Disease. IX ed Elsevier, (anche ed italiana)
Kumar et al Basic Pathology Elsevier
Gallo, D'Amati: anatomia Patologica UTET

The student must be able to understand the importance of the various anatomopathological methods (cytology, histoenzymatic histology and molecular biology) as tools of diagnostic, prognostic and predictive significance in the main chronic tumor and inflammatory pathologies. In particular, it will have to understand the impact of the technical laboratory activities on the histo-cytopathological diagnostic quality.

Notions of normal human anatomy and normal histology, basics of molecular biology; principles of treatment of biological materials

Power-point presentation

Oral test

Introduction to anatomical-pathological diagnostics: principles of macro and microscopic diagnostics, autopsy, histological and cytological.
Principles of treatment of cytological and histological materials; conservation of materials, fixation concepts.
Intraoperative diagnostics
Main methods of extraction of genomic material from tissues, main methods of extractive and in situ molecular diagnostics (FISH)
Lung, breast, colorectal, prostate, thyroid, urinary tract, biliary tract, central nervous system cancer: cyto-histological diagnostic approach and main molecular markers of prognostic-predictive interest.
Cutaneous melanomas: diagnostic principles and prognostic-predictive markers
Mammary sentinel node and in case of melanoma: main methods of analysis.
Chronic gastritis, celiac disease, Hirschsprung: diagnostic principles and ancillary techniques useful for diagnostic purposes.

At the end of the course the student must be able to understand the meaning of histo-cytopathological and molecular techniques and methods for predictive diagnostic and prognostic purposes