Ateneo - UPO

Histopathological lab: team and goals, safey, working procedures, how to handle the material;
Fixation, macroscopic examination and processing of histological and cytological material; morphological and histochemical stains in routine use; frozen section exam; general overview of digial pathology and tissue microarray construction (TMA) in surgical pathology

Bancroft's Theory and Practice of Histological Techniques
Book • Eighth Edition • 2019
Basic and Advanced Laboratory Techniques in Histopathology and Cytology, Springer, II ed.
Anatomia patologica. Le basi, Volume 1. Aldo Scarpa, Luigi Ruco. Edra, 2007

Demonstrate adequate knowledge of the principles governing the various phases of the tissue handling, fixation, and processing
Knowledge of the main morphological and histochemical staining techniques with their main applications
Knowledge in handling and following the frozen section procedure.

Knowledge of microscopic and macroscopic human anatomy and basic principles of biochemistry

Power-Point presentations

Multiple choice test

How to handle histological material; fixation procedures;
Sampling of histological material. The processing, Inclusion and cutting techniques. Frozen section examination and the cryostate utilisation. Staining with hematoxylin-eosin. Applications of dyes for connective tissue, mucopolysaccharides, lipids, amyloid, pigments, microorganisms.

Knowledge of the surgical pathology lab procedure, from the material arrival to the lab to the creation of a stained slides.
Knowledge of the principals morphological and histochemical stains, and how to use them.
Knowledge of the procedures and instruments used in the frozen section tissue examination.