Ateneo - UPO

Cells in their different morphologic and functional aspects. Techniques used to study cells and their components. Eucaryotic cell culture methods

Citologia e Istologia Funzionale di Calligaro A et al., edi-ermes
Files prepared by the professor.

At the end of the course the student must know the structure and function of the different cell organelles, as well as the principles of the techniques mostly used to study, isolate and culture the eucatiotic cells.

The student must have the basic knowledge of cell biology obtained from their previous secondary school.

Frontal lectures in the classroom, with the use of files in presentation format and video.

Students with physical disabilities, Learning Disabilities or Special Education Needs can request specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse e agli Studenti, consulting the University webpage: https://uniupo.it/en/services/services-students-physical-or-learning-disabilities. Students with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects.

Quiz with multiple choice questions (15 - 20 questions) on paper support.
As part of the Human Body course the test result will be taken in account for the definition of the final total exam score

-the eukariotic cell.
-the cell membrane: structure, function and specialization.
-the active transport through membranes: permeases, channels and pumps.
-cell membrane receptors.
-cell adhesion.
-the cytoskeleton.
-intracell compartments: endoplasmic reticulum, Golgi apparatus, lysosomes, perixosomes.
- the “sorting” within the cell: role of signal sequences in the establishing of cell compartments and protein destiny.
-exocytosis and endocytosis.
-the nucleus.
-mitocondria.
-apoptosis.
-techniques for cell study
-the microscopic observation. The optic microscope, the transmission electronic microscopy (TEM), the scanning electronic microscopy (SEM).
- The flow cytometry
-cell fractionation and separation of its components.
-characteristics and properties of stem cells.

Ability to recognize and describe the main constituent elements of a eukaryotic cell. Knowledge of basic methods for the study of eukaryotic cells. Knowledge of basic stem cell biology