Ateneo - UPO

The course aims to provide students with basic knowledge of the structure of DNA, replication, transcription, translation and the main techniques used to study DNA and RNA.

Slides and other didactic material provided by the teacher; online public database resources.
Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter. Molecular Biology of the Cell. Sixth Edition, ISBN: 978-0-393-87094-7

The aim of the course is to provide students with an overview of the structure of DNA and the mechanisms that govern fundamental phases of cellular processes: replication, repair, transcription and translation.
This basic knowledge is necessary to address future curricular topics in Regenerative Medicine.

Students are expected to have a general knowledge of biochemistry and cell biology.

Frontal lessons with PowerPoint presentations.
Running problems, quizzes and case resolution at home using the Wooclap platform.

All the teaching material and information regarding the course will be available on moodle DIR platform (https://www.dir.uniupo.it/).
Students with physical disabilities, Learning Disabilities or Special Education Needs can request
specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse

e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/services-
students-physical-or-learning-disabilities

Students with disabilities, learning disabilities or special education needs, once they have contacted
the University Staff, can refer to the tutor in charge of the course to define the examination
modalities, concerning academic aspects.

None

The structure and function of DNA:
chromosomal DNA and its packaging in the chromatin. Chromatin structure and function. DNA replication mechanisms. DNA repair. Transcription: initiation, elongation, and RNA processing. Translation. Analyzing and manipulating DNA and RNA: restriction nucleases, gel electrophoresis, cloning PCR.

Knowledge of the structure of DNA and the main basic cellular processes: replication, transcription and translation. Deepen the main molecular mechanisms in order to understand the implication of alterations of these mechanisms in the onset of diseases and how some of these can be exploited to correct any defects.