Ateneo - UPO

The course involves the preparation of competent bacterial cells for the uptake of exogenous DNA in the laboratory. This is followed by bacterial transformation and verification of gene amplification by PCR (colony PCR). The introduction of exogenous DNA into bacterial cells is assessed by agarose gel electrophoresis and immunofluorescence techniques.

There are no reference textbooks; only teaching materials provided by the instructor.

The aim of the proposed course is to provide students with a deeper understanding of molecular biology, with particular emphasis on its biotechnological applications.

Students are expected to have developed strong study skills and a solid command of biotechnological techniques throughout their academic journey. The programme is designed to support and reward the most deserving and motivated students, further enhancing their academic education and professional development.

Lectures combined with practical laboratory activities.

Assessment of learning: a critical-experimental approach. Students with physical disabilities, Learning Disabilities or Special Education Needs can request specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/services-students-physical-or-learning-disabilities. Students with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects.
The course adopts inclusive language and integrates a gender perspective into biomedical research, encouraging students to consider biological and social differences in experimental design, thereby promoting critical awareness and scientific rigor.

At the end of the course, students will have gained a deeper understanding of molecular analysis methods and their applications in biotechnology.

The course involves the preparation of competent bacterial cells for the uptake of exogenous DNA in the laboratory. This is followed by bacterial transformation and verification of gene amplification by PCR (colony PCR). The introduction of exogenous DNA into bacterial cells is assessed by agarose gel electrophoresis and immunofluorescence techniques.

The course provides students with a solid theoretical foundation in the main molecular methodologies used in biomedical research, including DNA analysis techniques integrated with cell and molecular biology approaches.