Ateneo - UPO

theoretical principles and some practical aspects of the main recombinant DNA techniques

- Jeremy Dale, et al. "Dai Geni ai Genomi" - EDISES (terza edizione) - James D. Watson et al. "DNA Ricombinante" - Zanichelli (seconda edizione) - Terry A. Brown "Biotecnologie Molecolari" - Zanichelli (seconde edizione)

Provide the theoretical and practical bases of the methods and technologies used in the study and analysis of gene expression, with particular emphasis on recombinant DNA techniques used to clone, express and analyze gene products of biomedical interest and applications. Importantly, the frequency of a dedicated laboratory is mandatory.

The student must know both cellular biology and basic genetics, and in particular the molecular and cellular mechanisms regulating replication, cell growth and gene expression programs in both prokaryotic and eukaryotic organisms.

classroom and laboratory lessons

Further teaching and support material is available on the DIR platform.

Specific services and tools will be provided to students with physical disabilities, learning disabilities or special educational needs.

written test (multiple-choice questions)

Concept of cloning. Knowledge of the main strategies for gene manipulation. Key features of cloning vectors. Expression of recombinant proteins. Knowledge of the main strategies for the creation and maintenance of genetically modified organisms. Knowledge of technologies applied to genetic diagnosis. Cloning strategies and analysis of gene libraries. PCR: principles and applications. Site-directed mutagenesis. Methods for gene transformation. Expression of transgenes in model animals. Introduction to proteomics. Genetic strategies for the phenotypic improvement of organisms of socio-economic interest. Animal & plant pharming. Cloning and DNA analysis in forensic science and archaeology.

The student will become familiar with the main methods and technologies used to study and analyze gene expression, together with the main techniques used to clone, express and analyze gene products of biomedical interest and application. Acquired familiarity with instrumentation and working methods of a research / analysis laboratory.