Ateneo - UPO

The course will be held both in class and in the laboratory. The course includes the study and application of the most advanced proteomics and metabolomics techniques employed in biomedical and biotechnological fields. Skills on methodologies and tools used in chemical and biological laboratories, for solving biomedical and biotechnological problems will be provided. The potential and weaknesses of the various methods will be illustrated through examples on complex biological matrices. The latest technological developments will be also treated. Several proteomics and metabolomics experiment will be prepared, including mass spectrometry analysis, data elaboration and bioinformatics.

There is no required textbook. Scientific papers and slides, provided during the lessons, will represent the main teaching materials. Further suggested books:
- Mirzaei Hamid, Carrasco Martin (Eds.): “Modern Proteomics – Sample Preparation, Analysis and Practical Applications”, Springer International Publishing
- Sanjoy K. Bhattacharya: “Metabolomics “, Humana Press

The course provides to the students a deepening of the most advanced proteomics and metabolomics techniques that will allow them to solve biological problems and interpret the results. The students will acquire interdisciplinary skills that will allow them to collaborate with colleagues from biological, chemical and medical areas.

Basic biochemistry, biology and laboratory techniques.

Frontal lessons and laboratory experiences.

Learning control: Discussion of topics during class.
Students with physical disabilities, Learning Disabilities or Special Education Needs can request specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/services-students-physical-or-learning-disabilities
Students with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects.

Oral examination. The test consists of a variable number of questions regarding the whole program and the discussion of a presentation / report prepared by the student on a topic previously agreed with the teacher. The overall assessment will take account of the Presentation / Report prepared by the student and of the marks obtained in individual applications.

Introduction to the course, learning of the principles of operation of the modern scientific instrumentation and laboratory testing. Targeted and untargeted proteomics and metabolomics.
Lab experience: Sample preparation from different matrixes (biological fluids, cells, etc.) and analysis with mass spectrometry. Statistical analysis of large datasets and interpretation of results.
Bioinformatics analysis

In order to reach the knowledge and skills corresponding to the minimum level of sufficiency, the student should demonstrate:
Knowledge and understanding:
- to know the basic principles of theoretical-practical operation of the techniques and methodologies presented during the course;
- know the theoretical-practical principles in order to use the appropriate techniques for the analysis of biological samples.
Ability to apply knowledge and understanding:
- ability to choose, depending on the problem proposed, what is the best approach to the solution;
Communication skills:
Ability to communicate clear and exhaustive methods of analysis
To achieve an advanced level, the student must:
- be able to solve a biomedical problem with the most suitable technique.