Ateneo - UPO

Review of general topics of Molecular Biology relevant to the understanding of the course. Chromatin Remodeling: Histone Modifications, DNA methylation Structural classes of transcription factors (Myc, Fos) Epigenetic definition: epigenetic diseases and epigenetic therapies. Oncogenes and tumor suppressor genes. P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function. Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions. Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells. Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep). Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency. Parallelisms between epigenetic reprogramming and cancer. Therapeutic use of iPS Cells and embryonic stem cells.

Molecular Biology of the Cell Alberts Scientific Papers

Provide basic knowledge of stem cell biology, reprogramming and pluripotency with particular reference to bio-medical applications, their research perspectives and the understanding of the major cellular processes.

A basic understanding of cell biology and molecular biology.

Lessons in the classroom supported by the projection of films aimed at helping the learning process. Development of classroom problems involving the use of concepts and tools illustrated during lectures. Practise. To help students in their study and preparation for the final exam, deliveries will be assigned during the course which will contribute to a minimal part of the final grade.

Altre informazioni/Further information ALTRO 4000 Sì Controllo dell’apprendimento in itinere mediante test interattivi in aula utilizzando la piattaforma Wooclap. Le studentesse e gli studenti con disabilità o con Disturbi Specifici dell’Apprendimento (DSA) o con Bisogni Educativi Speciali (BES) possono richiedere servizi e strumenti specifici a loro dedicati rivolgendosi allo Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse e agli Studenti e consultando la pagina dedicata del sito di Ateneo: https://uniupo.it/it/servizi/servizi-studenti- disabili-e-dsa Le studentesse e gli studenti con disabilità, DSA, BES, una volta preso contatto con lo Staff di Ateneo, possono contattare la/il docente titolare dell'insegnamento in relazione alla declinazione delle modalità di esame, in merito agli aspetti didattici. Test of ongoing learning, through interactive tests in the classroom using the wooclap platform. Students with physical disabilities, Learning Disabilities or Special Education Needs can request specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/services- students-physical-or-learning-disabilities Students with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects.

Written exam and evaluation of assignments (preparation of scientific posters and presentations) concerning scientific articles assigned to students during the course.

Programma esteso/Content PROGR_EST 8050 Sì Ripasso di argomenti generali di Biologia Molecolare rilevanti per la comprensione del corso. Rimodellamento della cromatina: modificazioni degli istoni, metilazione del DNA Fattori di trascrizione Definizione epigenetica: malattie epigenetiche e terapie epigenetiche. Oncogeni e geni oncosoppressori. Funzione di P53 nelle cellule normali e tumorali. MDM2 come oncogene. Funzione di pRb. Eterogeneità dei tumori, teorie stocastiche e gerarchiche, proprietà delle cellule staminali tumorali, loro isolamento, divisioni asimmetriche e simmetriche. Vie di segnalazione attivate nelle cellule staminali tumorali, implicazioni terapeutiche delle cellule staminali tumorali. Riprogrammazione di cellule somatiche a pluripotenza mediante trasferimento nucleare di cellule somatiche (SCNT) in ovociti. Fusione tra cellule somatiche e pluripotenti ed espressione ectopica di fattori di trascrizione definiti. Pecore clonate mediante trasferimento nucleare (pecora Dolly). Cellule staminali pluripotenti indotte (cellule iPS); papers di Yamanaka; riprogrammazione da fattori di trascrizione; cause di bassa efficienza di riprogrammazione. Parallelismi tra riprogrammazione epigenetica e cancro. Uso terapeutico delle cellule iPS e delle cellule staminali embrionali. Review of general topics of Molecular Biology relevant to the understanding of the course. Chromatin Remodeling: Histone Modifications, DNA methylation Structural classes of transcription factors (Myc, Fos) Epigenetic definition: epigenetic diseases and epigenetic therapies. Oncogenes and tumor suppressor genes. P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function. Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions. Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells. Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep). Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency. Parallelisms between epigenetic reprogramming and cancer. Therapeutic use of iPS Cells and embryonic stem cells. Programma esteso/Content PROGR_EST 8050 Sì Ripasso di argomenti generali di Biologia Molecolare rilevanti per la comprensione del corso. Rimodellamento della cromatina: modificazioni degli istoni, metilazione del DNA Fattori di trascrizione Definizione epigenetica: malattie epigenetiche e terapie epigenetiche. Oncogeni e geni oncosoppressori. Funzione di P53 nelle cellule normali e tumorali. MDM2 come oncogene. Funzione di pRb. Eterogeneità dei tumori, teorie stocastiche e gerarchiche, proprietà delle cellule staminali tumorali, loro isolamento, divisioni asimmetriche e simmetriche. Vie di segnalazione attivate nelle cellule staminali tumorali, implicazioni terapeutiche delle cellule staminali tumorali. Riprogrammazione di cellule somatiche a pluripotenza mediante trasferimento nucleare di cellule somatiche (SCNT) in ovociti. Fusione tra cellule somatiche e pluripotenti ed espressione ectopica di fattori di trascrizione definiti. Pecore clonate mediante trasferimento nucleare (pecora Dolly). Cellule staminali pluripotenti indotte (cellule iPS); papers di Yamanaka; riprogrammazione da fattori di trascrizione; cause di bassa efficienza di riprogrammazione. Parallelismi tra riprogrammazione epigenetica e cancro. Uso terapeutico delle cellule iPS e delle cellule staminali embrionali. Review of general topics of Molecular Biology relevant to the understanding of the course. Chromatin Remodeling: Histone Modifications, DNA methylation Structural classes of transcription factors (Myc, Fos) Epigenetic definition: epigenetic diseases and epigenetic therapies. Oncogenes and tumor suppressor genes. P53 function in normal and tumoral cells. MDM2 as an oncogene. pRb function. Heterogeneity of tumors, stochastic and hierarchical theories, properties of cancer stem cells, their isolation, asymmetric and symmetric divisions. Signaling Pathways activated in cancer stem cells, therapeutic implications of cancer stem cells. Reprogramming of somatic cells to pluripotency by somatic cell nuclear transfer (SCNT) into oocytes. Fusion between somatic and pluripotent cells and ectopic expression of defined transcription factors. Sheep cloned by nuclear transfer (Dolly sheep). Induced pluripotent stem cells (iPS cells); Yamanaka papers; reprogramming by transcription factors; causes of low reprogramming efficiency. Parallelisms between epigenetic reprogramming and cancer. Therapeutic use of iPS Cells and embryonic stem cells.

Provide basic knowledge of stem cell biology, reprogramming and pluripotency with particular reference to bio-medical