Ateneo - UPO

Fundamental elements of analytical instrumentation (spectroscopic techniques, mass spectrometry, atomic spectroscopies, chromatography, electrophoretic methods), and their use for analytical purposes. See “Programma esteso” (Extended Program) for more detailed information.

Rubinson/Rubinson, "Chimica Analitica Strumentale", Zanichelli;
Skoog/Holler/Crouch, "Chimica analitica strumentale", EdiSES;
D.A. Skoog e J.J. Leary, “Chimica analitica strumentale”, EdiSES;
notes provided by the teacher.

The course ha the objective of providing the students with robust knowledge on the theoretical bases of the most modern instrumental analytical techniques, on the evaluation of their performances, and on the problems normally faced in the analytical laboratories. Abilities: to know how to face and solve analytical problems; to know how to compare different techniques for the same purpose also by means of the evaluation of the indexes of analytical performance.Communication skills: acquire and use an appropriate technical language acquire and know how to use an appropriate chemical vocabulary in relation to the topics covered in the course; know how to expose a case study using multimedia tools. Ability in making judgements: ability in choosing the best analytical technique to solve a given analytical problem, also by critically comparing the indexes of analytical performance.Learning skills: ability in performing a bibliographic search by online search choosing the most reliable sources and ability in using autonomously the learning material to solve case studies.

None.

Lessons, notes and powerpoint presentations, classroom discussions, check during the course by DIR platform.

The learning during the course will be evaluated by classroom discussions on the topics already faced in the course and by tests about the acquired level of knowledge at the end of each topic by DIR platform.
Students with physical disabilities, Learning Disabilities or Special Education Needs can request specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/servicesstudents-physical-or-learning-disabilities.
Students with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects.

Oral examination to evaluate the student’s skills in managing the theoretical aspects of the instrumental analytical techniques and verify his ability in choosing the most suitable technique to solve a practical problem. The exam consists in three questions, chosen among: the description of an analytical technique for what regards both the instrumental and the theoretical aspects; the description of performance parameters of an analytical method and/or calibration methods; choice of the most suitable analytical procedure for the determination of a particular class of analytes in a real sample.The oral examination also comprises the discussion of a case study, given the student during the course, by a powerpoint presentation: the student has to autonomously make a bibliographic search to solve a given case study and present the best solution by means of a powerpoint presentation of about 5-10 minutes, followed by discussion.

The validation of analytical methods, quality parameters of analytical methods (LOD, LOQ, uncertainty of measurement, dynamic and linear range, robustness, precision and trueness, repeatability and reproducibility, recovery). Theory of calibration and the most exploited calibration methods (external standards, standard additions, internal standards). General survey on chromatography: separation mechanisms, chromatogram, performance parameters of a chromatographic separation, theoretical plates theory and velocity theory, optimization of separations, classification, paper and thin layer chromatography. Gaschromatography: instrumentation, sample injection, columns, separation mechanisms, detectors. Liquid chromatography (HPLC): mechanisms, instrumentation, sample injection, columns, detectors. Spectroscopic techniques: electromagnetic spectra and types of spectroscopies. UV-vis molecular spectroscopy: instrumentation, detectors, qualitative and quantitative applications. Elemental analysis (flame and graphite furnace atomic absorbment, ICP-MS, ICP-OES): sample introduction, sample pretreatment, instrumentation, analytical performance, applications. Mass spectrometry: instrumentation, ion sources, coupling to GC, HPLC and ICP, sample introduction, mass analyzers (quadrupole, magnetic sector, ion trap, ICR, TOF, hybrid), detector, acquisition methods, types of mass spectra and interpretation, use of libraries. Other techniques of separation and identification. Electrophoresis (capillary and in gel). Hints on sample pre-treatment and sampling methods. Execution of a bibliographic research by search in online scientific databases, approach to the solution of case studies and to the effective presentation of the results by multimedia.

Knowledge and understanding
- solid knowledge of the theoretical and theoretical / practical bases of the most modern instrumental analytical techniques (spectroscopic techniques, mass spectrometry, chromatographic and electrophoretic methods)
- knowledge of the analytical performance indices and calibration methods
- basic knowledge of sample pre-treatment and sampling methods
- knowledge of the main online databases available in the University and how to solve a case study and present it

Ability to apply knowledge and understanding
- be able to face and solve analytical problems;
- be able to compare different techniques for the same purpose, also by analyzing the analytical performance indexes
- be able to perform a bibliographic search through the online databases available at the University
- be able to organize the presentation of a case study

Communication skills
- acquire and use an appropriate chemical vocabulary in relation to the topics covered in the course
- be able to expose a case study using multimedia tools.

Autonomy of judgment
- ability to choose the best analytical technique to solve a given analytical problem
- be able to critically compare indices of analytical performance

Learning ability
- ability to perform a bibliographic search through online research choosing the most reliable sources and to use the study material independently to solve case studies