Ateneo - UPO

General introduction of genomics applied to the study of tumors.
Isolation of nucleic acids. DNA electrophoresis. DNA cloning, plasmid vector, restriction enzymes.
Polymerase chain reaction (PCR), and its applications. Southern blot and Northern blot.
Protein electrophoresis and western blotting.
Microarray technology and RNA sequencing.
DNA sequencing methods.
Next Generation Sequencing and its clinical applications.
Chromatin immunoprecipitation (CHIP).
Flow cytometry, general principles and its applications (apoptosis and cell cycle studies).

Biologia molecolare. Principi e tecniche di Michael M. Cox et al Zanichelli
Scientific Publications

Provide the general principles on which the main molecular techniques used in clinical diagnostics and research are based.

Basic knowledge of molecular biology mainly in eukaryotic organisms

Handouts, teaching materials, PowerPoint presentations of the lessons and scientific articles provided by the teacher

No additional information

Written test with multiple choice questions.

General introduction of genomics applied to the study of tumors.

Isolation of nucleic acids (DNA and RNA), purification, and calculation of the concentration.

DNA electrophoresis. DNA cloning, plasmid vector, restriction enzymes. Bacterial transformation.

Polymerase chain reaction (PCR), quantitative PCR.
Use of DNA intercalated dyes capable of emitting fluorescence (SYBER-Green) or hybridization probes linked to fluorescent molecules for a specific sequence approach (probes
TaqMan). Applications in research and care.

Southern blot and Northern blot: general principles and their applications
Protein electrophoresis, western blotting.

Chromatin Immunoprecipitation (CHIP)

Description of the general principles of the DNA microarray technique (use for comparison and
grouping). Applications of genomics (molecular medicine, forensic medicine, pharmacogenomics, study of multifactorial diseases). RNA sequencing (advantages over microarrays).

DNA sequencing methods (Sanger method).
Next-generation sequencing: general principles and clinical applications of next-generation sequencing techniques.

Pharmacogenomics and personalized therapy

Flow cytometry, general principles (monoclonal antibodies
related to fluorescent substances, multi-parametric analysis, sorting) and its applications (apoptosis and cell cycle studies).

The student must know the general principles of molecular biology and molecular biology techniques used in research and in the clinic