Ateneo - UPO

cell culture laboratory
concept of sterility
use of cell cultures
microscopic observation of cultured cells
expansion of a cell culture

During the lessons, students are provided with the slides along with the accompanynig explorations to copy onto USB drive.

Ricognize a confluent cell culture.Prepare a cell suspension, and count the cells in a Burker chember.

Students must learn the basic techniques required to work in a cell culture laboratory.

Oral explanation with the aid of a Power Point presentation.

There is no other information.

The course is passed via a multiple-choice test, each question is worth 2 points.

The cell culture laboratory: equipment and cell handling. The concept of sterility. Primary cultures and cell lines, growth phass and enzymatic dissociation.
Contamination. Freezing and thawing cell storage.

Knowing everithing a cell need to grow and multiply, as well as how cell cultures are astablished, and how they are stored