Ateneo - UPO

Citogenetics Hystory Cytogenetics techniques and applications Karyotyping and Chromosomal banding Normal and aberrant karyotypepostnatal and prenatal cytogeneticscytogenetics in tumorsMolecular cytogenetics: Fluorescent in situ Hybridization (FISH) and molecular karyotyping

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Students will be able to know principles and applications of cytogenetics procedures and analysis. Students must know limits and advantages of each tecnique.

Students must have basic genetics and molecular biology knowledge

Frontal lessons Interactive activitiesLaboratory activities

Students with physical disabilities, Learning Disabilities or Special Education Needs can request specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/ students-physical-or-learning-disabilitiesStudents with disabilities, learning disabilities or special education needs, once they have contacted the University Staff, can refer to the tutor in charge of the course to define the examination modalities, concerning academic aspects

Written exam including both multiple-choice quizzes and open-ended questions.

Cytogenetics History Hypotonic solution and colchicine Mitosis and meiosis Banding teqniques and resolution Karyotype analysis, advantages and limits Normal and disease Karyotype Chromosomal aberrations: atypical number of chromosomes or a structural abnormality Prenatal chromosomal and postnatal Molecular cytogenetics; Fluorescent in situ hybridization (FISH), advantages and disadvantages The choice of probes. Probe labelling Chromosome staining dyes Molecular karyotyping : principles, resolution, limits and advantages Copy number variations (CNV) : common polymorphisms versus pathological CNVCNV databases Guidelines for Cytogenetics analysis: Cytogenetics laboratory equipment and resolution Karyotype analysis, advantages and limitsNormal and disease KaryotypeChromosomal aberrations: atypical number of chromosomes or a structural abnormality Prenatal chromosomal and postnatal Molecular cytogenetics; Fluorescent in situ hybridization (FISH), advantages and disadvantages The choice of probes. Probe labelling Chromosome staining dyes Molecular karyotyping : prinicples, resolution, limits and advantages Copy number variations (CNV) : common polymorphisms versus pathological CNV CNVs databases Guidelines for Cytogenetics analysis: Cytogenetics laboratory equipment

Students should be able to know limits and advantages of each cytogenetic procedures and their applications