Ateneo - UPO

The course, after a review of basic immunology and the explanation of the basic concepts of flow cytometry, addresses the topics of multiparametric cytometry, scRNA-seq and mass image analysis. Focusing the attention of the course on single cell analysis techniques through the use of different algorithms.

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The course aims to apply single cell analysis techniques using different algorithms. Applying these techniques to multiparametric cytometry, image mass cytometry and scRNA-seq.

Knowledge on basic Immunological processes

Frontal lessons

Students with physical disabilities, Learning Disabilities or Special Education Needs can request

specific services and tools via the Staff Sviluppo e Coordinamento Carriere e Servizi alle Studentesse

e agli Studenti, consulting the University webpage: https://www.uniupo.it/en/services/services-

students-physical-or-learning-disabilities

Students with disabilities, learning disabilities or special education needs, once they have contacted

the University Staff, can refer to the tutor in charge of the course to define the examination

modalities, concerning academic aspects.

Written exam with questions with true/false answers and subsequent explanation

Flow cytometry: basic concepts and its applications. scRNA-seq: basic concepts and applications. Image mass cytometry: basic concepts and its applications. Single cell analysis techniques using different algorithms (dimensionality reduction, clustering algorithm, classification algorithm). SEURAT pipeline

Students will have to demonstrate knowledge of the main single cell techniques and complex data analysis techniques (multiparametric cytometry, scRNA-seq and image mass cytometry)