Ateneo - UPO

Provide the theoretical and practical bases of the methods and technologies usedin the study and analysis of gene expression, with particular emphasis onrecombinant DNA techniques used to clone, express and analyze gene productsof biomedical interest and applications. Importantly, the frequency of a dedicatedlaboratory is mandatory.

The student must know both cellular biology and basic genetics, and in particularthe molecular and cellular mechanisms regulating replication, cell growth and geneexpression programs in both prokaryotic and eukaryotic organisms.

Further teaching and support material is available on the DIRplatform.
Specific services and tools will be provided to studentswith physical disabilities, learning disabilities or special educationalneeds.

Written test (multiple-choice questions)

Concept of cloning. Knowledge of the main strategies for gene manipulation. Keyfeatures of cloning vectors. Expression of recombinant proteins. Knowledge of themain strategies for the creation and maintenance of genetically modified organisms.Knowledge of technologies applied to genetic diagnosis. Cloning strategies andanalysis of gene libraries. PCR: principles and applications. Site-directedmutagenesis. Methods for gene transformation. Expression of transgenes in modelanimals. Introduction to proteomics. Genetic strategies for the phenotypicimprovement of organisms of socio-economic interest. Animal & plant pharming.Cloning and DNA analysis in forensic science and archaeology.